| Definition | Vibrio harveyi ATCC BAA-1116 chromosome II, complete sequence. |
|---|---|
| Accession | NC_009784 |
| Length | 2,204,018 |
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The map label for this gene is hsdM [H]
Identifier: 156978013
GI number: 156978013
Start: 1927441
End: 1929021
Strand: Reverse
Name: hsdM [H]
Synonym: VIBHAR_06810
Alternate gene names: 156978013
Gene position: 1929021-1927441 (Counterclockwise)
Preceding gene: 156978014
Following gene: 156978012
Centisome position: 87.52
GC content: 46.68
Gene sequence:
>1581_bases TTGAAAAAATTTAACGCTATTCGAGATAACTTAATGCCCCAGTCTATTAATGAGATTGCTCCAATCAACCAAGACCCTCT TTACCAAGATCAAGTCAACAAAGCCGTCTGGTCAGCGTGCGATACGTTCCGTGGCACCGTAGATCCATCTATCTACAAAG ACTTTATTTTGACCATGCTGTTTTTAAAATACATCTCCGATGTACGCCAAGACAAAGTCGAAGAACTCACTACACAGTTC GGTGACAACAAAGAGATGGTTGAAGCCATGCTGGCGAGCCAATCTTTCAAAATTCCAGCAGGCTCAACTTTCTGGGATTT GTACGAAGCCCGCTTTGAAGCAGGTAATGGCTCTCGTATTGACCAAGCATTGCATGCGATTGAAGAAGCCAACGGCACCA AACTGAAAGGCGTGTTCCAAGACATCAGCTTTAACACCGATAAACTGGGTGATGAAAAGCAAAAGAACGACATCCTACGC CACCTATTGGAAGACTTTGGTAAGCCAACCCTAAACCTACGCCCAAGCCGTGTTGGCTCGCTGGATGTGATTGGTAACGC CTACGAATACCTGATCAAACACTTTGCGGCTGGCAGCGGTAAATCGGCAGGTGAATTCTATACTCCGCCAGAGGTGTCGG ATCTGCTTTCTATCATCCTAGAGCCACAACAAGGTGACAGCATTTGTGACCCTGCATGTGGTTCAGGCTCGCTATTGATG AAGTGTGGTAAACAGGTTCAAAAGAACTTTGGTGGCTCTAAGCAGTATGCCCTGTTTGGTCAAGAAGCGATTGGTTCAAC GTGGTCATTGGCGAAAATGAACATGTTTCTGCATGGCGAAGACAACCACCGCATTGAATGGGGCGACACCATTCGCAACC CTAAGCTGCAAGACAGCAACGGTGGCTTACTGCACTTTGATGTTGTAACCGCAAACCCACCGTTCTCGTTAGATAAATGG GGGCATGAAGATGCAGAAAACGATCACTTTGGTCGTTTCCGCCGTGGCGTGCCACCAAAAACCAAAGGCGACTACGCATT CATCTCACACATGATTGAAACCCTGAAACCAGAGACAGGTCGTATGGGCGTGGTTGTGCCTCACGGTGTGCTATTCCGTG CATCTTCTGAGGGTAAAATCCGTAAACAGTTGATCGAAGAAAACTTGCTAGATGCGGTGATCGGCTTACCTGAAAAACTA TTCTTTGGTACGGGCATTCCTGCGGCAATCTTGATCTTCAAAAAGAAGAAAGACACCAACGATGTCATGTTCATTGATGC CTCACGAGAGTTCAAGTCAGGCAAAAACCAGAACGTTCTTACGGCAGAAAACATCGACAAGATTGTTAAGACCTACCGCA GTGGCGACAACGTGGATAAGTACGCGTATGTAGCAACGCTCGATGAGATCCGTGAGAACGACTACAACCTGAATATTCCT CGTTACGTGGATACGTTTGAAGAAGAAGCAGAAATCGATTTGATGGCTGTGCGTAGTGAGCGATTGGCATTACAAACAGA GTTAGCCGATCTAGAAGCAGAAATGAAGGGCTACCTAGAGGAGCTGGGTTATGGTGCCTAA
Upstream 100 bases:
>100_bases CAACGATTTCAGGACTTCGTCACGCCAATGGTGTTTAACACCGAACCCCATGATTTTGATACCTGCTTTGCCTCATTTAA ACGCATTGCAGCATCGCTAA
Downstream 100 bases:
>100_bases TCAATATTTGCCATCTCATCTTGGTGAGATCCCAAATCATTGGAAAAGAGTGCGCTTTCTTGATGTCATGAAAGTTCGAC AAGGATTACAAATTGCACTT
Product: type I restriction enzyme M protein
Products: NA
Alternate protein names: M.EcoR124II [H]
Number of amino acids: Translated: 526; Mature: 526
Protein sequence:
>526_residues MKKFNAIRDNLMPQSINEIAPINQDPLYQDQVNKAVWSACDTFRGTVDPSIYKDFILTMLFLKYISDVRQDKVEELTTQF GDNKEMVEAMLASQSFKIPAGSTFWDLYEARFEAGNGSRIDQALHAIEEANGTKLKGVFQDISFNTDKLGDEKQKNDILR HLLEDFGKPTLNLRPSRVGSLDVIGNAYEYLIKHFAAGSGKSAGEFYTPPEVSDLLSIILEPQQGDSICDPACGSGSLLM KCGKQVQKNFGGSKQYALFGQEAIGSTWSLAKMNMFLHGEDNHRIEWGDTIRNPKLQDSNGGLLHFDVVTANPPFSLDKW GHEDAENDHFGRFRRGVPPKTKGDYAFISHMIETLKPETGRMGVVVPHGVLFRASSEGKIRKQLIEENLLDAVIGLPEKL FFGTGIPAAILIFKKKKDTNDVMFIDASREFKSGKNQNVLTAENIDKIVKTYRSGDNVDKYAYVATLDEIRENDYNLNIP RYVDTFEEEAEIDLMAVRSERLALQTELADLEAEMKGYLEELGYGA
Sequences:
>Translated_526_residues MKKFNAIRDNLMPQSINEIAPINQDPLYQDQVNKAVWSACDTFRGTVDPSIYKDFILTMLFLKYISDVRQDKVEELTTQF GDNKEMVEAMLASQSFKIPAGSTFWDLYEARFEAGNGSRIDQALHAIEEANGTKLKGVFQDISFNTDKLGDEKQKNDILR HLLEDFGKPTLNLRPSRVGSLDVIGNAYEYLIKHFAAGSGKSAGEFYTPPEVSDLLSIILEPQQGDSICDPACGSGSLLM KCGKQVQKNFGGSKQYALFGQEAIGSTWSLAKMNMFLHGEDNHRIEWGDTIRNPKLQDSNGGLLHFDVVTANPPFSLDKW GHEDAENDHFGRFRRGVPPKTKGDYAFISHMIETLKPETGRMGVVVPHGVLFRASSEGKIRKQLIEENLLDAVIGLPEKL FFGTGIPAAILIFKKKKDTNDVMFIDASREFKSGKNQNVLTAENIDKIVKTYRSGDNVDKYAYVATLDEIRENDYNLNIP RYVDTFEEEAEIDLMAVRSERLALQTELADLEAEMKGYLEELGYGA >Mature_526_residues MKKFNAIRDNLMPQSINEIAPINQDPLYQDQVNKAVWSACDTFRGTVDPSIYKDFILTMLFLKYISDVRQDKVEELTTQF GDNKEMVEAMLASQSFKIPAGSTFWDLYEARFEAGNGSRIDQALHAIEEANGTKLKGVFQDISFNTDKLGDEKQKNDILR HLLEDFGKPTLNLRPSRVGSLDVIGNAYEYLIKHFAAGSGKSAGEFYTPPEVSDLLSIILEPQQGDSICDPACGSGSLLM KCGKQVQKNFGGSKQYALFGQEAIGSTWSLAKMNMFLHGEDNHRIEWGDTIRNPKLQDSNGGLLHFDVVTANPPFSLDKW GHEDAENDHFGRFRRGVPPKTKGDYAFISHMIETLKPETGRMGVVVPHGVLFRASSEGKIRKQLIEENLLDAVIGLPEKL FFGTGIPAAILIFKKKKDTNDVMFIDASREFKSGKNQNVLTAENIDKIVKTYRSGDNVDKYAYVATLDEIRENDYNLNIP RYVDTFEEEAEIDLMAVRSERLALQTELADLEAEMKGYLEELGYGA
Specific function: Methylation of specific adenine residues; required for both restriction and modification activities. The EcoR124/3 I enzyme recognizes 5'-GAAN(7)RTCG-3' [H]
COG id: COG0286
COG function: function code V; Type I restriction-modification system methyltransferase subunit
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]
Homologues:
Organism=Escherichia coli, GI1790808, Length=479, Percent_Identity=24.2171189979123, Blast_Score=105, Evalue=5e-24,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR022749 - InterPro: IPR003356 - InterPro: IPR002052 - InterPro: IPR002296 - InterPro: IPR004546 [H]
Pfam domain/function: PF12161 HsdM_N; PF02384 N6_Mtase [H]
EC number: =2.1.1.72 [H]
Molecular weight: Translated: 58991; Mature: 58991
Theoretical pI: Translated: 4.90; Mature: 4.90
Prosite motif: PS00636 DNAJ_1
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.8 %Cys (Translated Protein) 2.5 %Met (Translated Protein) 3.2 %Cys+Met (Translated Protein) 0.8 %Cys (Mature Protein) 2.5 %Met (Mature Protein) 3.2 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MKKFNAIRDNLMPQSINEIAPINQDPLYQDQVNKAVWSACDTFRGTVDPSIYKDFILTML CCCHHHHHHHCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH FLKYISDVRQDKVEELTTQFGDNKEMVEAMLASQSFKIPAGSTFWDLYEARFEAGNGSRI HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCHHH DQALHAIEEANGTKLKGVFQDISFNTDKLGDEKQKNDILRHLLEDFGKPTLNLRPSRVGS HHHHHHHHHCCCCEEHHHHHHCCCCCHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCC LDVIGNAYEYLIKHFAAGSGKSAGEFYTPPEVSDLLSIILEPQQGDSICDPACGSGSLLM HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCHHHH KCGKQVQKNFGGSKQYALFGQEAIGSTWSLAKMNMFLHGEDNHRIEWGDTIRNPKLQDSN HHHHHHHHHCCCCCEEEEECHHHCCCCCCCEEEEEEEECCCCCEEECCCCCCCCCCCCCC GGLLHFDVVTANPPFSLDKWGHEDAENDHFGRFRRGVPPKTKGDYAFISHMIETLKPETG CCEEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCC RMGVVVPHGVLFRASSEGKIRKQLIEENLLDAVIGLPEKLFFGTGIPAAILIFKKKKDTN CEEEEECCCEEEEECCCCHHHHHHHHHHHHHHHHCCCHHHHHCCCCCEEEEEEECCCCCC DVMFIDASREFKSGKNQNVLTAENIDKIVKTYRSGDNVDKYAYVATLDEIRENDYNLNIP CEEEEECCHHHCCCCCCCCEEHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCC RYVDTFEEEAEIDLMAVRSERLALQTELADLEAEMKGYLEELGYGA HHHHHHHHHCCEEHEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC >Mature Secondary Structure MKKFNAIRDNLMPQSINEIAPINQDPLYQDQVNKAVWSACDTFRGTVDPSIYKDFILTML CCCHHHHHHHCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH FLKYISDVRQDKVEELTTQFGDNKEMVEAMLASQSFKIPAGSTFWDLYEARFEAGNGSRI HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCHHH DQALHAIEEANGTKLKGVFQDISFNTDKLGDEKQKNDILRHLLEDFGKPTLNLRPSRVGS HHHHHHHHHCCCCEEHHHHHHCCCCCHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCC LDVIGNAYEYLIKHFAAGSGKSAGEFYTPPEVSDLLSIILEPQQGDSICDPACGSGSLLM HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCHHHH KCGKQVQKNFGGSKQYALFGQEAIGSTWSLAKMNMFLHGEDNHRIEWGDTIRNPKLQDSN HHHHHHHHHCCCCCEEEEECHHHCCCCCCCEEEEEEEECCCCCEEECCCCCCCCCCCCCC GGLLHFDVVTANPPFSLDKWGHEDAENDHFGRFRRGVPPKTKGDYAFISHMIETLKPETG CCEEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCC RMGVVVPHGVLFRASSEGKIRKQLIEENLLDAVIGLPEKLFFGTGIPAAILIFKKKKDTN CEEEEECCCEEEEECCCCHHHHHHHHHHHHHHHHCCCHHHHHCCCCCEEEEEEECCCCCC DVMFIDASREFKSGKNQNVLTAENIDKIVKTYRSGDNVDKYAYVATLDEIRENDYNLNIP CEEEEECCHHHCCCCCCCCEEHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCC RYVDTFEEEAEIDLMAVRSERLALQTELADLEAEMKGYLEELGYGA HHHHHHHHHCCEEHEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 2784505 [H]