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The map label for this gene is ldhA [H]

Identifier: 156977971

GI number: 156977971

Start: 1897393

End: 1898502

Strand: Direct

Name: ldhA [H]

Synonym: VIBHAR_06767

Alternate gene names: 156977971

Gene position: 1897393-1898502 (Clockwise)

Preceding gene: 156977969

Following gene: 156977972

Centisome position: 86.09

GC content: 43.78

Gene sequence:

>1110_bases
ATGGTGATTCTAAAGCAAACTGAATATGTGCAAATTTCGCTACTCTTTGTAACGAACTTGGATTACTTTATAAGGATATT
ACTTTATAAATCCAGCACAAATAGAGAGGGTATTATGCTCAACATCGCTTTTTTCAGTGCAAAATCCTATGACGAAGCGT
CTTTTAACAAAATCAAAAATCATCGTGATTTCGAATTCCATTTCCATGATTTTCGATTGACCTCTAAGACTGCCAAAATG
GCACAAGGCTGCGAAGTGGTGTGCGCATTTGTAAATGACGATTTGTCAGCTCCAGTACTTAAACAGCTTTCTCTCGGTGG
CACTAAGTTGATCGCCATGCGTTGTGCAGGCTTTGATAAGGTAGATTTAAAAGCAGCAAAAGAGTTCGGGCTGCAAGTGG
TGCGCGTCCCTGCTTATTCGCCAGAGGCGGTCGCAGAGCACACCGTCGGTATGATGATGTGTTTAAACAGACGCCTACAT
AAAGCATACCAACGTACTCGTGATGCTAACTTCAACTTGGAAGGCTTAGTGGGTTTTAACTTCCATGGCAAAACCGTAGG
TGTCGTCGGTACGGGCAAAATTGGTATCGCAACCATGCGCATCTTTAAAGGTTTGGGCATGGAAATACTATGCTACGATC
CATATGAAAACCCACTTGCGTTGGAAATGGGCGCACGCTACTGCTCTCTTGAAGAAATCTATGCGCAGGCCGATGTTATT
TCTTTGCACTGCCCAATGAGCAAAGAAAACTATCACCTCCTCAACGAGAATTCCTTCTCGCAAATGAAAGATGGCGTAAT
GATCATCAATACCAGCCGTGGCGAGTTACTAGACTCCGTGGCGGCCATCGAAGCATTGAAACAAGGCAAGATTGGTGCAC
TTGGTTTGGACGTCTATGACAATGAGAAAGACTTATTCTTCCAAGACAAATCAAATGACGTAATTGTTGATGACGTATTC
CGCCGTTTATCTGCATGCCACAACGTATTGTTCACTGGGCACCAAGCATTCTTGACTCATGAAGCCCTGAATAACATCGC
GTCCGTAACTCTAAGCAACGTGGATGCTTTCTTTACCGGCAACCCGTCAGGTAATGAACTGATCAACTAA

Upstream 100 bases:

>100_bases
TACAACATAAGATTAAAGACAACCATTTTACTGACATCGCCAGAACGCCTAACTAACCCACTCATAGGGAAAACAAAAAA
AGAGAACCCATGCTAGTCAG

Downstream 100 bases:

>100_bases
ATCAATAGATCGAGCAGTATAGTAGAGCGTTGAATTTCAGCTATGCTATACTCCTCGACCATTAAAAATTTGAGTCCAAT
TACCATGAGCATAAAAACCG

Product: D-lactate dehydrogenase

Products: NA

Alternate protein names: D-LDH; Fermentative lactate dehydrogenase [H]

Number of amino acids: Translated: 369; Mature: 369

Protein sequence:

>369_residues
MVILKQTEYVQISLLFVTNLDYFIRILLYKSSTNREGIMLNIAFFSAKSYDEASFNKIKNHRDFEFHFHDFRLTSKTAKM
AQGCEVVCAFVNDDLSAPVLKQLSLGGTKLIAMRCAGFDKVDLKAAKEFGLQVVRVPAYSPEAVAEHTVGMMMCLNRRLH
KAYQRTRDANFNLEGLVGFNFHGKTVGVVGTGKIGIATMRIFKGLGMEILCYDPYENPLALEMGARYCSLEEIYAQADVI
SLHCPMSKENYHLLNENSFSQMKDGVMIINTSRGELLDSVAAIEALKQGKIGALGLDVYDNEKDLFFQDKSNDVIVDDVF
RRLSACHNVLFTGHQAFLTHEALNNIASVTLSNVDAFFTGNPSGNELIN

Sequences:

>Translated_369_residues
MVILKQTEYVQISLLFVTNLDYFIRILLYKSSTNREGIMLNIAFFSAKSYDEASFNKIKNHRDFEFHFHDFRLTSKTAKM
AQGCEVVCAFVNDDLSAPVLKQLSLGGTKLIAMRCAGFDKVDLKAAKEFGLQVVRVPAYSPEAVAEHTVGMMMCLNRRLH
KAYQRTRDANFNLEGLVGFNFHGKTVGVVGTGKIGIATMRIFKGLGMEILCYDPYENPLALEMGARYCSLEEIYAQADVI
SLHCPMSKENYHLLNENSFSQMKDGVMIINTSRGELLDSVAAIEALKQGKIGALGLDVYDNEKDLFFQDKSNDVIVDDVF
RRLSACHNVLFTGHQAFLTHEALNNIASVTLSNVDAFFTGNPSGNELIN
>Mature_369_residues
MVILKQTEYVQISLLFVTNLDYFIRILLYKSSTNREGIMLNIAFFSAKSYDEASFNKIKNHRDFEFHFHDFRLTSKTAKM
AQGCEVVCAFVNDDLSAPVLKQLSLGGTKLIAMRCAGFDKVDLKAAKEFGLQVVRVPAYSPEAVAEHTVGMMMCLNRRLH
KAYQRTRDANFNLEGLVGFNFHGKTVGVVGTGKIGIATMRIFKGLGMEILCYDPYENPLALEMGARYCSLEEIYAQADVI
SLHCPMSKENYHLLNENSFSQMKDGVMIINTSRGELLDSVAAIEALKQGKIGALGLDVYDNEKDLFFQDKSNDVIVDDVF
RRLSACHNVLFTGHQAFLTHEALNNIASVTLSNVDAFFTGNPSGNELIN

Specific function: Fermentative lactate dehydrogenase [H]

COG id: COG1052

COG function: function code CHR; Lactate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI23308577, Length=199, Percent_Identity=34.6733668341709, Blast_Score=133, Evalue=2e-31,
Organism=Homo sapiens, GI4557497, Length=246, Percent_Identity=32.1138211382114, Blast_Score=126, Evalue=3e-29,
Organism=Homo sapiens, GI61743967, Length=246, Percent_Identity=32.1138211382114, Blast_Score=126, Evalue=3e-29,
Organism=Homo sapiens, GI145580578, Length=261, Percent_Identity=29.1187739463602, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI4557499, Length=261, Percent_Identity=29.1187739463602, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI145580575, Length=261, Percent_Identity=29.1187739463602, Blast_Score=122, Evalue=6e-28,
Organism=Homo sapiens, GI6912396, Length=267, Percent_Identity=28.0898876404494, Blast_Score=101, Evalue=9e-22,
Organism=Escherichia coli, GI1787645, Length=329, Percent_Identity=64.1337386018237, Blast_Score=453, Evalue=1e-129,
Organism=Escherichia coli, GI87082289, Length=273, Percent_Identity=28.5714285714286, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI1789279, Length=241, Percent_Identity=30.7053941908714, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI1788660, Length=201, Percent_Identity=27.8606965174129, Blast_Score=64, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17532191, Length=204, Percent_Identity=28.921568627451, Blast_Score=110, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI25147481, Length=242, Percent_Identity=30.1652892561983, Blast_Score=109, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6324055, Length=196, Percent_Identity=32.6530612244898, Blast_Score=119, Evalue=9e-28,
Organism=Saccharomyces cerevisiae, GI6322116, Length=233, Percent_Identity=30.4721030042918, Blast_Score=116, Evalue=4e-27,
Organism=Saccharomyces cerevisiae, GI6320925, Length=233, Percent_Identity=29.6137339055794, Blast_Score=112, Evalue=9e-26,
Organism=Saccharomyces cerevisiae, GI6324964, Length=212, Percent_Identity=29.2452830188679, Blast_Score=99, Evalue=9e-22,
Organism=Saccharomyces cerevisiae, GI6324980, Length=140, Percent_Identity=30, Blast_Score=74, Evalue=5e-14,
Organism=Drosophila melanogaster, GI24646446, Length=262, Percent_Identity=32.0610687022901, Blast_Score=131, Evalue=6e-31,
Organism=Drosophila melanogaster, GI24646448, Length=262, Percent_Identity=32.0610687022901, Blast_Score=131, Evalue=6e-31,
Organism=Drosophila melanogaster, GI24646452, Length=262, Percent_Identity=32.0610687022901, Blast_Score=131, Evalue=6e-31,
Organism=Drosophila melanogaster, GI24646450, Length=262, Percent_Identity=32.0610687022901, Blast_Score=131, Evalue=6e-31,
Organism=Drosophila melanogaster, GI62472511, Length=262, Percent_Identity=32.0610687022901, Blast_Score=131, Evalue=8e-31,
Organism=Drosophila melanogaster, GI19921140, Length=262, Percent_Identity=29.3893129770992, Blast_Score=106, Evalue=3e-23,
Organism=Drosophila melanogaster, GI28574286, Length=261, Percent_Identity=26.8199233716475, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI45552429, Length=260, Percent_Identity=26.1538461538462, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24585514, Length=260, Percent_Identity=26.1538461538462, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI28574282, Length=260, Percent_Identity=26.1538461538462, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI28574284, Length=260, Percent_Identity=26.1538461538462, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI45551003, Length=260, Percent_Identity=26.1538461538462, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI28571528, Length=265, Percent_Identity=26.4150943396226, Blast_Score=85, Evalue=8e-17,
Organism=Drosophila melanogaster, GI24585516, Length=209, Percent_Identity=23.9234449760766, Blast_Score=68, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006139
- InterPro:   IPR006140
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00389 2-Hacid_dh; PF02826 2-Hacid_dh_C [H]

EC number: =1.1.1.28 [H]

Molecular weight: Translated: 41225; Mature: 41225

Theoretical pI: Translated: 6.58; Mature: 6.58

Prosite motif: PS00065 D_2_HYDROXYACID_DH_1 ; PS00670 D_2_HYDROXYACID_DH_2 ; PS00671 D_2_HYDROXYACID_DH_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVILKQTEYVQISLLFVTNLDYFIRILLYKSSTNREGIMLNIAFFSAKSYDEASFNKIKN
CEEEECCCEEEEEEEEECCHHHHEEEEEEECCCCCCCEEEEEEEEECCCCCCHHHHHHCC
HRDFEFHFHDFRLTSKTAKMAQGCEVVCAFVNDDLSAPVLKQLSLGGTKLIAMRCAGFDK
CCCEEEEEEEEEEEHHHHHHHCCCEEEEEEECCCCCCHHHHHHCCCCCEEEEEEECCCCC
VDLKAAKEFGLQVVRVPAYSPEAVAEHTVGMMMCLNRRLHKAYQRTRDANFNLEGLVGFN
HHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEE
FHGKTVGVVGTGKIGIATMRIFKGLGMEILCYDPYENPLALEMGARYCSLEEIYAQADVI
ECCCEEEEEECCCHHHHHHHHHHCCCCEEEEECCCCCCEEEECCCCCCCHHHHHHCCCEE
SLHCPMSKENYHLLNENSFSQMKDGVMIINTSRGELLDSVAAIEALKQGKIGALGLDVYD
EEECCCCCCCEEEECCCCHHHHHCCEEEEECCCHHHHHHHHHHHHHHCCCCEEEEEEEEC
NEKDLFFQDKSNDVIVDDVFRRLSACHNVLFTGHQAFLTHEALNNIASVTLSNVDAFFTG
CCCCEEEECCCCCEEHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHEEECCCEEEEEC
NPSGNELIN
CCCCCCCCC
>Mature Secondary Structure
MVILKQTEYVQISLLFVTNLDYFIRILLYKSSTNREGIMLNIAFFSAKSYDEASFNKIKN
CEEEECCCEEEEEEEEECCHHHHEEEEEEECCCCCCCEEEEEEEEECCCCCCHHHHHHCC
HRDFEFHFHDFRLTSKTAKMAQGCEVVCAFVNDDLSAPVLKQLSLGGTKLIAMRCAGFDK
CCCEEEEEEEEEEEHHHHHHHCCCEEEEEEECCCCCCHHHHHHCCCCCEEEEEEECCCCC
VDLKAAKEFGLQVVRVPAYSPEAVAEHTVGMMMCLNRRLHKAYQRTRDANFNLEGLVGFN
HHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEE
FHGKTVGVVGTGKIGIATMRIFKGLGMEILCYDPYENPLALEMGARYCSLEEIYAQADVI
ECCCEEEEEECCCHHHHHHHHHHCCCCEEEEECCCCCCEEEECCCCCCCHHHHHHCCCEE
SLHCPMSKENYHLLNENSFSQMKDGVMIINTSRGELLDSVAAIEALKQGKIGALGLDVYD
EEECCCCCCCEEEECCCCHHHHHCCEEEEECCCHHHHHHHHHHHHHHCCCCEEEEEEEEC
NEKDLFFQDKSNDVIVDDVFRRLSACHNVLFTGHQAFLTHEALNNIASVTLSNVDAFFTG
CCCCEEEECCCCCEEHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHEEECCCEEEEEC
NPSGNELIN
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9025293; 9097039; 9278503; 8349564 [H]