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The map label for this gene is lhgO [H]

Identifier: 156977920

GI number: 156977920

Start: 1847759

End: 1848991

Strand: Reverse

Name: lhgO [H]

Synonym: VIBHAR_06715

Alternate gene names: 156977920

Gene position: 1848991-1847759 (Counterclockwise)

Preceding gene: 156977921

Following gene: 156977919

Centisome position: 83.89

GC content: 48.58

Gene sequence:

>1233_bases
ATGAAATCAGCCTATGACTACATCATTGTTGGTGGCGGTATCGTTGGTGTGTCGACAGCATGGCAACTGCAGCAACGTCA
TCCAGACAAATCCATTTTATTGGTTGAGAAAGAGTCCGGTTTTGCCAAGCACCAAACCGGACATAATAGTGGCGTGATTC
ATGCCGGGGTGTACTACGCACCGGGCAGCTTAAAGGCAGATTTTTGTAAGCGAGGGGTAGAGCGAACCATTTCGTTCTGC
GCCGAGCACGATATTCCGGTGGAGAACTGTGGCAAGTTACTGGTTGCTACCAATGAGCAAGAAGTCGAACGTATGAATGC
CCTCTATGAGCGCTGCCATGTCAACGGCATCGACGTAAAGCTACTCGATGAAGTTCAACTCAAGCTGGCTGAGCCAAACA
TCACAGGCTTGGGCGCGATTTATGTCAAAACCACCAGTATTGTCGATTATCGTTTGGTCACTGAGCAGATGGCGGAAGAG
TTCCAAAAGCTCGGTGGTCAAATCAGCCTGAGAACCAAAGTAGTGGCTGCCGAAGAGAAGGATGAAGAAGTCCAACTGAC
CTGCACTTCAGACGGTCAAACCATGCAGCTCAATGCCAAATTCTTGGTCACTTGCAGTGGCTTGATGGCGGATCGCATGA
CTAAGATGATGGGTATTCTAACCGATTTCCAAATCATCCCTTATCGCGGCGAATACTACCGTTTGGATAGTAAGCACGAC
CAAGTCGTGAATCATCTTATTTACCCAATCCCAGATCCTGATTTGCCTTTTTTGGGTGTCCACCTCACTCGTATGATTGA
TGGTAGTGTGACCGTTGGTCCAAATGCGGTGCAAGGCTGGAAGCGAGAAGGGTACGATAAGGTCAACTTTAGCCTGCAAG
ATACCATGCAAATGCTCAGCTTTTCAGGTTTCTGGAAGGTGACACAAAAGCATCTAAAAACCGGATTGGATGAGTTTAAG
AACTCGTGGTGGAAGCCGGGCTATCTCAAGCTGGTCAATAAATACTGCCCAAGCATCAAGGTGGAAGATCTCAAACCATA
TCCTGCGGGTATTCGTGCGCAAGCGGTGTTGTCCGATGGCACTTTGGTTCATGACTTCCTGTTTGCCGAGAGCGCAAGAA
GCCTACACGTTTGCAACGCACCCTCTCCCGCAGCGACATCTGCAATGCCGATTGGGGAGTACATTTGCGATAAAGTGAAT
GAGAAGATAGAGGCAAAAACCGAGGCGGTTTAA

Upstream 100 bases:

>100_bases
TTCGTGTAGCAAGCTGCGCAGTATTTGTCGCGATTTTTATTTACAGTGGTCGCAAGTTCAAATTGCAACAATCTCAACTA
TCGGTTTTATAAGAGGTCGC

Downstream 100 bases:

>100_bases
CAGTAAAAAGCCAAACTAGAAGTACCCAAGAAAAATAATGACTTTGTTTGTTAGGTTATTAGTGTCGGGTGTGCTGGAAG
TTTGGCTTTTACTTTGGTGA

Product: hydroxyglutarate oxidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 410; Mature: 410

Protein sequence:

>410_residues
MKSAYDYIIVGGGIVGVSTAWQLQQRHPDKSILLVEKESGFAKHQTGHNSGVIHAGVYYAPGSLKADFCKRGVERTISFC
AEHDIPVENCGKLLVATNEQEVERMNALYERCHVNGIDVKLLDEVQLKLAEPNITGLGAIYVKTTSIVDYRLVTEQMAEE
FQKLGGQISLRTKVVAAEEKDEEVQLTCTSDGQTMQLNAKFLVTCSGLMADRMTKMMGILTDFQIIPYRGEYYRLDSKHD
QVVNHLIYPIPDPDLPFLGVHLTRMIDGSVTVGPNAVQGWKREGYDKVNFSLQDTMQMLSFSGFWKVTQKHLKTGLDEFK
NSWWKPGYLKLVNKYCPSIKVEDLKPYPAGIRAQAVLSDGTLVHDFLFAESARSLHVCNAPSPAATSAMPIGEYICDKVN
EKIEAKTEAV

Sequences:

>Translated_410_residues
MKSAYDYIIVGGGIVGVSTAWQLQQRHPDKSILLVEKESGFAKHQTGHNSGVIHAGVYYAPGSLKADFCKRGVERTISFC
AEHDIPVENCGKLLVATNEQEVERMNALYERCHVNGIDVKLLDEVQLKLAEPNITGLGAIYVKTTSIVDYRLVTEQMAEE
FQKLGGQISLRTKVVAAEEKDEEVQLTCTSDGQTMQLNAKFLVTCSGLMADRMTKMMGILTDFQIIPYRGEYYRLDSKHD
QVVNHLIYPIPDPDLPFLGVHLTRMIDGSVTVGPNAVQGWKREGYDKVNFSLQDTMQMLSFSGFWKVTQKHLKTGLDEFK
NSWWKPGYLKLVNKYCPSIKVEDLKPYPAGIRAQAVLSDGTLVHDFLFAESARSLHVCNAPSPAATSAMPIGEYICDKVN
EKIEAKTEAV
>Mature_410_residues
MKSAYDYIIVGGGIVGVSTAWQLQQRHPDKSILLVEKESGFAKHQTGHNSGVIHAGVYYAPGSLKADFCKRGVERTISFC
AEHDIPVENCGKLLVATNEQEVERMNALYERCHVNGIDVKLLDEVQLKLAEPNITGLGAIYVKTTSIVDYRLVTEQMAEE
FQKLGGQISLRTKVVAAEEKDEEVQLTCTSDGQTMQLNAKFLVTCSGLMADRMTKMMGILTDFQIIPYRGEYYRLDSKHD
QVVNHLIYPIPDPDLPFLGVHLTRMIDGSVTVGPNAVQGWKREGYDKVNFSLQDTMQMLSFSGFWKVTQKHLKTGLDEFK
NSWWKPGYLKLVNKYCPSIKVEDLKPYPAGIRAQAVLSDGTLVHDFLFAESARSLHVCNAPSPAATSAMPIGEYICDKVN
EKIEAKTEAV

Specific function: Oxidizes L-2-hydroxyglutarate, probably to recover alpha-ketoglutarate mistakenly reduced by other enzymes or formed during growth on propionate [H]

COG id: COG0579

COG function: function code R; Predicted dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the L2HGDH family [H]

Homologues:

Organism=Homo sapiens, GI13376331, Length=416, Percent_Identity=40.3846153846154, Blast_Score=298, Evalue=8e-81,
Organism=Escherichia coli, GI87082148, Length=404, Percent_Identity=55.9405940594059, Blast_Score=454, Evalue=1e-129,
Organism=Caenorhabditis elegans, GI17565764, Length=407, Percent_Identity=40.5405405405405, Blast_Score=324, Evalue=5e-89,
Organism=Drosophila melanogaster, GI24585081, Length=399, Percent_Identity=39.8496240601504, Blast_Score=293, Evalue=1e-79,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015657
- InterPro:   IPR006076 [H]

Pfam domain/function: PF01266 DAO [H]

EC number: =1.1.3.15 [H]

Molecular weight: Translated: 45703; Mature: 45703

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSAYDYIIVGGGIVGVSTAWQLQQRHPDKSILLVEKESGFAKHQTGHNSGVIHAGVYYA
CCCCCCEEEECCCEEECCHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCEEEEEEEEC
PGSLKADFCKRGVERTISFCAEHDIPVENCGKLLVATNEQEVERMNALYERCHVNGIDVK
CCCCCHHHHHHHHHHHHHHHHCCCCCHHHCCCEEEECCHHHHHHHHHHHHHHCCCCCCEE
LLDEVQLKLAEPNITGLGAIYVKTTSIVDYRLVTEQMAEEFQKLGGQISLRTKVVAAEEK
EEECEEEEEECCCCCCEEEEEEEEECHHHHHHHHHHHHHHHHHHCCEEEEEEEEEEECCC
DEEVQLTCTSDGQTMQLNAKFLVTCSGLMADRMTKMMGILTDFQIIPYRGEYYRLDSKHD
CCEEEEEECCCCCEEEECCEEEEEECHHHHHHHHHHHHHHHCCEEEECCCCEEEECCHHH
QVVNHLIYPIPDPDLPFLGVHLTRMIDGSVTVGPNAVQGWKREGYDKVNFSLQDTMQMLS
HHHHHEECCCCCCCCCHHHHHHHHHCCCCEEECCHHHHHHHHCCCCEECCCHHHHHHHHH
FSGFWKVTQKHLKTGLDEFKNSWWKPGYLKLVNKYCPSIKVEDLKPYPAGIRAQAVLSDG
CCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCEEECCCCCCCCCEEEEEECCC
TLVHDFLFAESARSLHVCNAPSPAATSAMPIGEYICDKVNEKIEAKTEAV
CCHHHHHHHHCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKSAYDYIIVGGGIVGVSTAWQLQQRHPDKSILLVEKESGFAKHQTGHNSGVIHAGVYYA
CCCCCCEEEECCCEEECCHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCEEEEEEEEC
PGSLKADFCKRGVERTISFCAEHDIPVENCGKLLVATNEQEVERMNALYERCHVNGIDVK
CCCCCHHHHHHHHHHHHHHHHCCCCCHHHCCCEEEECCHHHHHHHHHHHHHHCCCCCCEE
LLDEVQLKLAEPNITGLGAIYVKTTSIVDYRLVTEQMAEEFQKLGGQISLRTKVVAAEEK
EEECEEEEEECCCCCCEEEEEEEEECHHHHHHHHHHHHHHHHHHCCEEEEEEEEEEECCC
DEEVQLTCTSDGQTMQLNAKFLVTCSGLMADRMTKMMGILTDFQIIPYRGEYYRLDSKHD
CCEEEEEECCCCCEEEECCEEEEEECHHHHHHHHHHHHHHHCCEEEECCCCEEEECCHHH
QVVNHLIYPIPDPDLPFLGVHLTRMIDGSVTVGPNAVQGWKREGYDKVNFSLQDTMQMLS
HHHHHEECCCCCCCCCHHHHHHHHHCCCCEEECCHHHHHHHHCCCCEECCCHHHHHHHHH
FSGFWKVTQKHLKTGLDEFKNSWWKPGYLKLVNKYCPSIKVEDLKPYPAGIRAQAVLSDG
CCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCEEECCCCCCCCCEEEEEECCC
TLVHDFLFAESARSLHVCNAPSPAATSAMPIGEYICDKVNEKIEAKTEAV
CCHHHHHHHHCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 8297211; 7984428 [H]