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The map label for this gene is cry [H]

Identifier: 156977901

GI number: 156977901

Start: 1828297

End: 1829679

Strand: Reverse

Name: cry [H]

Synonym: VIBHAR_06693

Alternate gene names: 156977901

Gene position: 1829679-1828297 (Counterclockwise)

Preceding gene: 156977903

Following gene: 156977900

Centisome position: 83.02

GC content: 46.93

Gene sequence:

>1383_bases
ATGGAAAAACTCACACATGGAACGCTTTCTTTGAATAAAACAATCGGACTGTACTGGTTTACCAACGATTTGAGGATGCA
TGATAACCCGCTGCTCGCCCGAGCCAGTGAGGAAGTCGACGCATTAATATTTGTCTATTGTTATCCGAAACTGTCTCCAT
TTTTAGCGCACTTTGCGCAGGAATCGATGTTTGGGTCGGCTAAGCAGCAATTTTTAGATGAGTCATTACATTGCGTCAAT
CTGACCTTGAATGCGTTCGACCAGCGCTTACAGGTTGTTGACTTACATCCTTATCAAGCCATCAAACATGCTGTAGAGAA
GCTTTGCGTGACACATCTGTATTGTGATACGTTCCCTGGCTCAGAAGAGCGAGACGTGGTTGATCATATCCGCCAAGAAT
TAGAGCATTTAACGATTTGCCAGCAGGAAGTGCGCAGCCTACTTACCGTTGAAGACCTGCCATTTGCCCTTGAAGACTTG
CCTGACACTTTTACTAAGTTTCGTAAAAAGGCGGAGAACGTATCGCTGGAGGAACCATTCGCCACAGTAACGGCCTTACC
ACCAACCCCCTCAGGACTTGCCTTGCCAACGTTACGACTGATTCGCGACGTTAAGCCTTGCCTGTTTACTGGTGGAGAGC
AAGCAGGGCTTGAACACGCTCGCAGGTATTTTTCCTCGACATTGGCCAGTGAATATAAGCAGACGCGCAATGGCTTGGAT
GGTATGGATTACTCGACTAAGTTTTCTCCTTGGCTAGCTCATGGCTGCCTTTCGCCAAAAACTATTTATGCCATGCTCAA
GCGTTATGAACGTTTGAAGGGAGCAAACGAGTCGACCTACTGGATTTACTTTGAGCTATTATGGCGGGAGTACTTTTATT
GGTATGCCCGTCGACACAAACGACAGCTTTTCCGATTCGGTGGCATCCAAAATCAAGCACCACTCACCAGCTTTTATGCT
CATCGTTTTCAGCAATGGAAGAAAGGTGAAACGCCATTTCCTATCGTGAACGCGTGTATGCACCAACTTAATGAAACCGG
TTATATGTCGAATCGTGGCAGACAGTTGGTCGCCAGTTGTTTAGTGCATGAACTTGGTCTCGATTGGCGTTATGGCGCAG
CGTACTTTGAATCACAACTGGTTGATTATGATGTAGCATCGAACTGGGGAAATTGGCAATACTTAGCAGGCGTTGGTGCT
GATCCGCGTGGTTCACGACAATTCAACCTCGAAAAGCAAACCGAAATATACGATCCGCACATGGAGTTCATCGATAGGTG
GCAAGGTCGTTGTGCTGGCGCGAATTTGGATAGTGTAGATATGGTTGATTGGCCTATCGTAATGGGCAGCAGTGACCAAG
CTTCGTCACCGGAGAAACCGTAA

Upstream 100 bases:

>100_bases
TTAATTTTAACAAATTGTTTACCAAGGACTTTTGCTGAATAATTTGTAGACGTAACAATCCACAGTCTGATCGTAAAGCA
GCATTGTGCGTAAGAACTAC

Downstream 100 bases:

>100_bases
TGCAATTTGAAACCGTACGATTGATCCTGGGCGATCAACTTAACAGTGAGCACTCTTGGTTTCAGCAACCGGATAAGCAA
GTGCTTTACCTGATCGCGGA

Product: deoxyribodipyrimidine photolyase

Products: 2 pyrimidine residues (in DNA) [C]

Alternate protein names: NA

Number of amino acids: Translated: 460; Mature: 460

Protein sequence:

>460_residues
MEKLTHGTLSLNKTIGLYWFTNDLRMHDNPLLARASEEVDALIFVYCYPKLSPFLAHFAQESMFGSAKQQFLDESLHCVN
LTLNAFDQRLQVVDLHPYQAIKHAVEKLCVTHLYCDTFPGSEERDVVDHIRQELEHLTICQQEVRSLLTVEDLPFALEDL
PDTFTKFRKKAENVSLEEPFATVTALPPTPSGLALPTLRLIRDVKPCLFTGGEQAGLEHARRYFSSTLASEYKQTRNGLD
GMDYSTKFSPWLAHGCLSPKTIYAMLKRYERLKGANESTYWIYFELLWREYFYWYARRHKRQLFRFGGIQNQAPLTSFYA
HRFQQWKKGETPFPIVNACMHQLNETGYMSNRGRQLVASCLVHELGLDWRYGAAYFESQLVDYDVASNWGNWQYLAGVGA
DPRGSRQFNLEKQTEIYDPHMEFIDRWQGRCAGANLDSVDMVDWPIVMGSSDQASSPEKP

Sequences:

>Translated_460_residues
MEKLTHGTLSLNKTIGLYWFTNDLRMHDNPLLARASEEVDALIFVYCYPKLSPFLAHFAQESMFGSAKQQFLDESLHCVN
LTLNAFDQRLQVVDLHPYQAIKHAVEKLCVTHLYCDTFPGSEERDVVDHIRQELEHLTICQQEVRSLLTVEDLPFALEDL
PDTFTKFRKKAENVSLEEPFATVTALPPTPSGLALPTLRLIRDVKPCLFTGGEQAGLEHARRYFSSTLASEYKQTRNGLD
GMDYSTKFSPWLAHGCLSPKTIYAMLKRYERLKGANESTYWIYFELLWREYFYWYARRHKRQLFRFGGIQNQAPLTSFYA
HRFQQWKKGETPFPIVNACMHQLNETGYMSNRGRQLVASCLVHELGLDWRYGAAYFESQLVDYDVASNWGNWQYLAGVGA
DPRGSRQFNLEKQTEIYDPHMEFIDRWQGRCAGANLDSVDMVDWPIVMGSSDQASSPEKP
>Mature_460_residues
MEKLTHGTLSLNKTIGLYWFTNDLRMHDNPLLARASEEVDALIFVYCYPKLSPFLAHFAQESMFGSAKQQFLDESLHCVN
LTLNAFDQRLQVVDLHPYQAIKHAVEKLCVTHLYCDTFPGSEERDVVDHIRQELEHLTICQQEVRSLLTVEDLPFALEDL
PDTFTKFRKKAENVSLEEPFATVTALPPTPSGLALPTLRLIRDVKPCLFTGGEQAGLEHARRYFSSTLASEYKQTRNGLD
GMDYSTKFSPWLAHGCLSPKTIYAMLKRYERLKGANESTYWIYFELLWREYFYWYARRHKRQLFRFGGIQNQAPLTSFYA
HRFQQWKKGETPFPIVNACMHQLNETGYMSNRGRQLVASCLVHELGLDWRYGAAYFESQLVDYDVASNWGNWQYLAGVGA
DPRGSRQFNLEKQTEIYDPHMEFIDRWQGRCAGANLDSVDMVDWPIVMGSSDQASSPEKP

Specific function: May have a photoreceptor function. Binds DNA; probably functions as a transcriptional repressor [H]

COG id: COG0415

COG function: function code L; Deoxyribodipyrimidine photolyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DNA photolyase domain [H]

Homologues:

Organism=Homo sapiens, GI188536100, Length=408, Percent_Identity=26.4705882352941, Blast_Score=112, Evalue=6e-25,
Organism=Homo sapiens, GI4758072, Length=407, Percent_Identity=27.027027027027, Blast_Score=111, Evalue=1e-24,
Organism=Homo sapiens, GI188536103, Length=360, Percent_Identity=25, Blast_Score=89, Evalue=7e-18,
Organism=Escherichia coli, GI1786926, Length=428, Percent_Identity=28.5046728971963, Blast_Score=136, Evalue=3e-33,
Organism=Saccharomyces cerevisiae, GI6324962, Length=267, Percent_Identity=28.4644194756554, Blast_Score=96, Evalue=1e-20,
Organism=Drosophila melanogaster, GI17137248, Length=412, Percent_Identity=25, Blast_Score=101, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24585455, Length=412, Percent_Identity=25, Blast_Score=101, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002081
- InterPro:   IPR014133
- InterPro:   IPR006050
- InterPro:   IPR005101
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00875 DNA_photolyase; PF03441 FAD_binding_7 [H]

EC number: 4.1.99.3 [C]

Molecular weight: Translated: 53099; Mature: 53099

Theoretical pI: Translated: 6.41; Mature: 6.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKLTHGTLSLNKTIGLYWFTNDLRMHDNPLLARASEEVDALIFVYCYPKLSPFLAHFAQ
CCCCCCCCEEECCEEEEEEEECCCEECCCCHHHHCCCCCCEEEEEEECCCHHHHHHHHHH
ESMFGSAKQQFLDESLHCVNLTLNAFDQRLQVVDLHPYQAIKHAVEKLCVTHLYCDTFPG
HHHHHHHHHHHHHCCHHEEEEEHHHHHCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCC
SEERDVVDHIRQELEHLTICQQEVRSLLTVEDLPFALEDLPDTFTKFRKKAENVSLEEPF
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCHHHHHHHHHHHHCCCCCCCC
ATVTALPPTPSGLALPTLRLIRDVKPCLFTGGEQAGLEHARRYFSSTLASEYKQTRNGLD
CEEEECCCCCCCCHHHHHHHHHHCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
GMDYSTKFSPWLAHGCLSPKTIYAMLKRYERLKGANESTYWIYFELLWREYFYWYARRHK
CCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHHHH
RQLFRFGGIQNQAPLTSFYAHRFQQWKKGETPFPIVNACMHQLNETGYMSNRGRQLVASC
HHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHH
LVHELGLDWRYGAAYFESQLVDYDVASNWGNWQYLAGVGADPRGSRQFNLEKQTEIYDPH
HHHHHCCCCCHHHHHHHHHHHCCHHHCCCCCEEEEEECCCCCCCCCCCCCCHHHHHCCHH
MEFIDRWQGRCAGANLDSVDMVDWPIVMGSSDQASSPEKP
HHHHHHHCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCC
>Mature Secondary Structure
MEKLTHGTLSLNKTIGLYWFTNDLRMHDNPLLARASEEVDALIFVYCYPKLSPFLAHFAQ
CCCCCCCCEEECCEEEEEEEECCCEECCCCHHHHCCCCCCEEEEEEECCCHHHHHHHHHH
ESMFGSAKQQFLDESLHCVNLTLNAFDQRLQVVDLHPYQAIKHAVEKLCVTHLYCDTFPG
HHHHHHHHHHHHHCCHHEEEEEHHHHHCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCC
SEERDVVDHIRQELEHLTICQQEVRSLLTVEDLPFALEDLPDTFTKFRKKAENVSLEEPF
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCHHHHHHHHHHHHCCCCCCCC
ATVTALPPTPSGLALPTLRLIRDVKPCLFTGGEQAGLEHARRYFSSTLASEYKQTRNGLD
CEEEECCCCCCCCHHHHHHHHHHCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
GMDYSTKFSPWLAHGCLSPKTIYAMLKRYERLKGANESTYWIYFELLWREYFYWYARRHK
CCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHHHH
RQLFRFGGIQNQAPLTSFYAHRFQQWKKGETPFPIVNACMHQLNETGYMSNRGRQLVASC
HHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHH
LVHELGLDWRYGAAYFESQLVDYDVASNWGNWQYLAGVGADPRGSRQFNLEKQTEIYDPH
HHHHHCCCCCHHHHHHHHHHHCCHHHCCCCCEEEEEECCCCCCCCCCCCCCHHHHHCCHH
MEFIDRWQGRCAGANLDSVDMVDWPIVMGSSDQASSPEKP
HHHHHHHCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: ATP; FADH2; Flavin; pterin [C]

Metal ions: NA

Kcat value (1/min): 2.4 [C]

Specific activity: NA

Km value (mM): NA

Substrates: cyclobutadipyrimidine (in DNA) [C]

Specific reaction: cyclobutadipyrimidine (in DNA) = 2 pyrimidine residues (in DNA) [C]

General reaction: C-C-bond cleavage [C]

Inhibitor: yeast DNA [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12620739 [H]