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The map label for this gene is amsI [H]

Identifier: 156977874

GI number: 156977874

Start: 1802914

End: 1803417

Strand: Direct

Name: amsI [H]

Synonym: VIBHAR_06666

Alternate gene names: 156977874

Gene position: 1802914-1803417 (Clockwise)

Preceding gene: 156977873

Following gene: 156977875

Centisome position: 81.8

GC content: 43.85

Gene sequence:

>504_bases
GTGATCTGGAAAGGGCTTTATAAACAGAAGCTCTTTCCAATAGTGATAGATAAGGAACAATCTATGTTTTCAAACATTCT
CGTCGTTTGCGTTGGTAACATTTGCCGCTCCCCAACAGCAGAACGAATGCTTCAACAACTGCTTCCTAATAAAACCATTG
CGTCAGCTGGGATTGCAGTGGAAAAAAGTCGCTTGATAGGACGATCTGCAGATGAAACTGCAACGCTGATTGCATCAGAG
AAAGGTTTATCTTTAGAAGACCACAAGGCTCAGCAGCTGACTGCACAACTATGCTCGAAGCAAGATCTCATCCTTGTGAT
GGAACAAGGGCATATTGATGCGCTGACTGAAATCGTTCCCGAAGCAAGAGGCAAAACCATGCTATTTGGACATTGGATGA
ACTCTGTCGACATTCCAGACCCATACAGACAGAGCAGAGAAGCATTCGACCATGCGTTCTCACTGATTGAAAAGTCCGCT
CATGCATGGGCAAAAAAAATATAA

Upstream 100 bases:

>100_bases
GTCGTTGGAACCGTGTCATCGCACAGCTAGTACCAACAATTTCAGGATTCAATGAGCTTACAGAAGGTGTTCTACGCGTT
AGAAACTGGCCGTAATTCGA

Downstream 100 bases:

>100_bases
CTTCAGACTACTTGGCGAAACATAAAAATAATGGCTAACCACACTATAAGTTCATCTGCACCCACCGATGATGAAATCGA
TCTCGGTAAACTGTTTGGCA

Product: phosphatase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 167; Mature: 167

Protein sequence:

>167_residues
MIWKGLYKQKLFPIVIDKEQSMFSNILVVCVGNICRSPTAERMLQQLLPNKTIASAGIAVEKSRLIGRSADETATLIASE
KGLSLEDHKAQQLTAQLCSKQDLILVMEQGHIDALTEIVPEARGKTMLFGHWMNSVDIPDPYRQSREAFDHAFSLIEKSA
HAWAKKI

Sequences:

>Translated_167_residues
MIWKGLYKQKLFPIVIDKEQSMFSNILVVCVGNICRSPTAERMLQQLLPNKTIASAGIAVEKSRLIGRSADETATLIASE
KGLSLEDHKAQQLTAQLCSKQDLILVMEQGHIDALTEIVPEARGKTMLFGHWMNSVDIPDPYRQSREAFDHAFSLIEKSA
HAWAKKI
>Mature_167_residues
MIWKGLYKQKLFPIVIDKEQSMFSNILVVCVGNICRSPTAERMLQQLLPNKTIASAGIAVEKSRLIGRSADETATLIASE
KGLSLEDHKAQQLTAQLCSKQDLILVMEQGHIDALTEIVPEARGKTMLFGHWMNSVDIPDPYRQSREAFDHAFSLIEKSA
HAWAKKI

Specific function: May function as a phosphatase required for amylovoran (an exopolysaccharide that functions as a virulence factor) production [H]

COG id: COG0394

COG function: function code T; Protein-tyrosine-phosphatase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the low molecular weight phosphotyrosine protein phosphatase family [H]

Homologues:

Organism=Escherichia coli, GI1788375, Length=146, Percent_Identity=55.4794520547945, Blast_Score=175, Evalue=1e-45,
Organism=Escherichia coli, GI87081812, Length=148, Percent_Identity=47.972972972973, Blast_Score=146, Evalue=8e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000106
- InterPro:   IPR017867 [H]

Pfam domain/function: PF01451 LMWPc [H]

EC number: =3.1.3.48 [H]

Molecular weight: Translated: 18690; Mature: 18690

Theoretical pI: Translated: 8.09; Mature: 8.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIWKGLYKQKLFPIVIDKEQSMFSNILVVCVGNICRSPTAERMLQQLLPNKTIASAGIAV
CCCCHHHHHCCCCEEECCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHCCHH
EKSRLIGRSADETATLIASEKGLSLEDHKAQQLTAQLCSKQDLILVMEQGHIDALTEIVP
HHHHHHCCCCCHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHH
EARGKTMLFGHWMNSVDIPDPYRQSREAFDHAFSLIEKSAHAWAKKI
HCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MIWKGLYKQKLFPIVIDKEQSMFSNILVVCVGNICRSPTAERMLQQLLPNKTIASAGIAV
CCCCHHHHHCCCCEEECCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHCCHH
EKSRLIGRSADETATLIASEKGLSLEDHKAQQLTAQLCSKQDLILVMEQGHIDALTEIVP
HHHHHHCCCCCHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHH
EARGKTMLFGHWMNSVDIPDPYRQSREAFDHAFSLIEKSAHAWAKKI
HCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7596293 [H]