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The map label for this gene is trpI [H]

Identifier: 156977816

GI number: 156977816

Start: 1754332

End: 1755198

Strand: Reverse

Name: trpI [H]

Synonym: VIBHAR_06604

Alternate gene names: 156977816

Gene position: 1755198-1754332 (Counterclockwise)

Preceding gene: 156977821

Following gene: 156977815

Centisome position: 79.64

GC content: 48.44

Gene sequence:

>867_bases
ATGAGGCATCTTAAATCCTTTCATGTTTTCCATGTCGCAGCATCGTCTGCGAGTTTTAGTGAGGCAGCAAATAAGCTCAA
TATTACGCATGGCGCAGTGAGTAAGCAGATCAAAGTGTTGGAGACCTATCTGGCTCAGCCTCTGTTCTACAAACAGGGGC
GTAATATGCTGCTCACTAAAGAGGGTGAGATGCTGAAAAGCTATACCGAGCAAGCATTCCAAGCGTTGGAGACTGGCGTG
AACAAGCTGGTGCAGGAAAAGCATCAATACCTTGAAGTCTCTTGTGAGCCAACGTTAACCATGCGTTGGTTGATGCCGAG
GCTTTCCACGTTTAATGATGATTGTGACGCAGACGTTCGGCTTTCGACTGCGGGAGGGCCTGTAACGCTCGGCTCGACAG
GGCTTGCTATGGCAATTCGTCGAGATGACTTTGAGCCTTTGTATCACTACCCCAAAACCAAGCTGGTGGAAGAGTGGGTA
GGTCCAGTGTTTTCACCTGATTACTGGCAGCAAGTGAAAAGTGATTTGAACTCCGTGAAGCTATTACACAGCCAAACCAG
ACCTGAAGCTTGGCACGAATGGATGCAGCGATCTGGTGAAGCGTCGATAAACGGAAAAGCAGAACAGTCTTTCGCACACT
TTTATTTCTGCATTCAAGCTGCGGTGGATGGCTTAGGCGCGGCGATGGGTTCTTATCCTTTGGTGATGGACGATCTCAAA
CGTGGCAACCTTGTCGCGCCATTTGGTTTTGTGCCGTCAGGGCATGACTATGTCTTATTGACTCAAGACAAAGCACGTTC
TGAACTGGAACAAAAATTTGTCCATTGGCTCCAAAACCAAATGGCGGAGTGTGTGCCTTGCACCTAA

Upstream 100 bases:

>100_bases
GTTTCCTGTTTCTTCGTTGTTATGAATAGGGCAATTGTAGGTCGAGAGTTCGGAGACTATAATCGAAACAAATTCCACTA
AGTGTGAGAAAAAGTCACAG

Downstream 100 bases:

>100_bases
GAAACAGAGAGAGACCCTAGTCAGGGCGCGCTGAGTTACCTAGAGTGGTTGAATACGTGCTACATAATGGAAATTTAGGA
AATGGAAAACCTCAATATTG

Product: transcriptional regulator

Products: NA

Alternate protein names: TrpBA operon transcriptional activator [H]

Number of amino acids: Translated: 288; Mature: 288

Protein sequence:

>288_residues
MRHLKSFHVFHVAASSASFSEAANKLNITHGAVSKQIKVLETYLAQPLFYKQGRNMLLTKEGEMLKSYTEQAFQALETGV
NKLVQEKHQYLEVSCEPTLTMRWLMPRLSTFNDDCDADVRLSTAGGPVTLGSTGLAMAIRRDDFEPLYHYPKTKLVEEWV
GPVFSPDYWQQVKSDLNSVKLLHSQTRPEAWHEWMQRSGEASINGKAEQSFAHFYFCIQAAVDGLGAAMGSYPLVMDDLK
RGNLVAPFGFVPSGHDYVLLTQDKARSELEQKFVHWLQNQMAECVPCT

Sequences:

>Translated_288_residues
MRHLKSFHVFHVAASSASFSEAANKLNITHGAVSKQIKVLETYLAQPLFYKQGRNMLLTKEGEMLKSYTEQAFQALETGV
NKLVQEKHQYLEVSCEPTLTMRWLMPRLSTFNDDCDADVRLSTAGGPVTLGSTGLAMAIRRDDFEPLYHYPKTKLVEEWV
GPVFSPDYWQQVKSDLNSVKLLHSQTRPEAWHEWMQRSGEASINGKAEQSFAHFYFCIQAAVDGLGAAMGSYPLVMDDLK
RGNLVAPFGFVPSGHDYVLLTQDKARSELEQKFVHWLQNQMAECVPCT
>Mature_288_residues
MRHLKSFHVFHVAASSASFSEAANKLNITHGAVSKQIKVLETYLAQPLFYKQGRNMLLTKEGEMLKSYTEQAFQALETGV
NKLVQEKHQYLEVSCEPTLTMRWLMPRLSTFNDDCDADVRLSTAGGPVTLGSTGLAMAIRRDDFEPLYHYPKTKLVEEWV
GPVFSPDYWQQVKSDLNSVKLLHSQTRPEAWHEWMQRSGEASINGKAEQSFAHFYFCIQAAVDGLGAAMGSYPLVMDDLK
RGNLVAPFGFVPSGHDYVLLTQDKARSELEQKFVHWLQNQMAECVPCT

Specific function: Activates the expression of the trpBA genes encoding the two tryptophan synthase subunits. In the absence of the inducer (indoleglycerol phosphate), trpI binds upstream of the trpAB operon, overlapping its own promoter region [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=296, Percent_Identity=30.7432432432432, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI1786448, Length=286, Percent_Identity=27.2727272727273, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1788706, Length=300, Percent_Identity=29.3333333333333, Blast_Score=95, Evalue=5e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32541; Mature: 32541

Theoretical pI: Translated: 6.74; Mature: 6.74

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRHLKSFHVFHVAASSASFSEAANKLNITHGAVSKQIKVLETYLAQPLFYKQGRNMLLTK
CCCCHHEEEEEEECCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHCHHHHHCCCCEEEEC
EGEMLKSYTEQAFQALETGVNKLVQEKHQYLEVSCEPTLTMRWLMPRLSTFNDDCDADVR
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHCCCCCCCCCEE
LSTAGGPVTLGSTGLAMAIRRDDFEPLYHYPKTKLVEEWVGPVFSPDYWQQVKSDLNSVK
EECCCCCEEECCCCCEEEEECCCCCHHHHCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
LLHSQTRPEAWHEWMQRSGEASINGKAEQSFAHFYFCIQAAVDGLGAAMGSYPLVMDDLK
HHHHCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCC
RGNLVAPFGFVPSGHDYVLLTQDKARSELEQKFVHWLQNQMAECVPCT
CCCEECCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MRHLKSFHVFHVAASSASFSEAANKLNITHGAVSKQIKVLETYLAQPLFYKQGRNMLLTK
CCCCHHEEEEEEECCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHCHHHHHCCCCEEEEC
EGEMLKSYTEQAFQALETGVNKLVQEKHQYLEVSCEPTLTMRWLMPRLSTFNDDCDADVR
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHCCCCCCCCCEE
LSTAGGPVTLGSTGLAMAIRRDDFEPLYHYPKTKLVEEWVGPVFSPDYWQQVKSDLNSVK
EECCCCCEEECCCCCEEEEECCCCCHHHHCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
LLHSQTRPEAWHEWMQRSGEASINGKAEQSFAHFYFCIQAAVDGLGAAMGSYPLVMDDLK
HHHHCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCC
RGNLVAPFGFVPSGHDYVLLTQDKARSELEQKFVHWLQNQMAECVPCT
CCCEECCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8423001 [H]