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The map label for this gene is pncA [C]

Identifier: 156977789

GI number: 156977789

Start: 1725136

End: 1725828

Strand: Direct

Name: pncA [C]

Synonym: VIBHAR_06577

Alternate gene names: 156977789

Gene position: 1725136-1725828 (Clockwise)

Preceding gene: 156977788

Following gene: 156977793

Centisome position: 78.27

GC content: 47.62

Gene sequence:

>693_bases
GTGGCTAACGCAAGCATAGAGACAGACAAGGAGTCTGCCATGACCGTTCTAGTTAATTATCAAACCACTGCTGTGATCGA
TGTCGATCCAGAGAAAGGTTTCACCGAACTTTGCCCTGACGAACTGCCAGTTACTGGCGCATTAGAAATCGTTCCAGAGC
TGCTTAAGAACCACACGAAAGGTCGTTTGAAATTAGTCAGCCGTGACTTGCACCCACCAAAAGCGGCTTGGGATGCAGAA
ACGCCAGCAAATATGCTTGAGCCAGTTGGCCTGCCCAATGTGGATATTAAGTGGAACCGCCACTGTGTGCTTGGTACTAC
TGGGGTAGAACTACTAGACGGTCTACCACCTGTTCTGGAATACGATTTCCAAGTGAACAAAGGTATGGATCCAGACGCCC
ACCCCTACGGCATCTTTTTCCATGACGTGGCGGATACCAAAACCACCGGTACGAATGAGTTCTTAAAATTCAACCACATC
GACACCGTGGTTGTTGGTGGCTTGGCACTCGATTTTTGTGTGAAAAAGTCCGTCACTCAAGCTCTAGAGTTAGGTTTCAA
AGTGATTGTGAACCTAGCATCTAGCCGCGCGGTATTGCCAGATACTATCGATGCGGTCATTGCTGAAATGGAAGAGAAAG
GCGCGGTGTTTGTTAAAAATGCAGATGACATCATCGTAGAAAACTTCGCTTAG

Upstream 100 bases:

>100_bases
ACCAACAAAGTCGCTTCTACCAACAAAGGCAAAGGCGGTAAGCCAGCTATGGTGTACACCCTAGCAAGCGACGAAGTCAC
CTTTTTCCAAAACTGCTTGC

Downstream 100 bases:

>100_bases
TCCCCCTCTGAATAACAAAAAACGGTCCAGCAAAACTGGACCGTTTTTTTGTTTTTGCGTGTACTAAGCTTGGGGGATTA
CTTAATTACGCCACAAACCA

Product: pyrazinamidase/nicotinamidase

Products: nicotinate; NH3

Alternate protein names: Nicotinamidase; Isochorismatase Hydrolase; Isochorismatase Family Protein; Amidase; Pyrazinamidase; Pyrazinamidase Nicotinamidase; Pyrazinamidase/Nicotinamidase PncA; Pyrazinamidase / Nicotinamidase; Nicotinamidase/Pyrazinamidase; Pyrazinamidase/Nicotinamidase Protein; Bifunctional Pyrazinamidase/Nicotinamidase

Number of amino acids: Translated: 230; Mature: 229

Protein sequence:

>230_residues
MANASIETDKESAMTVLVNYQTTAVIDVDPEKGFTELCPDELPVTGALEIVPELLKNHTKGRLKLVSRDLHPPKAAWDAE
TPANMLEPVGLPNVDIKWNRHCVLGTTGVELLDGLPPVLEYDFQVNKGMDPDAHPYGIFFHDVADTKTTGTNEFLKFNHI
DTVVVGGLALDFCVKKSVTQALELGFKVIVNLASSRAVLPDTIDAVIAEMEEKGAVFVKNADDIIVENFA

Sequences:

>Translated_230_residues
MANASIETDKESAMTVLVNYQTTAVIDVDPEKGFTELCPDELPVTGALEIVPELLKNHTKGRLKLVSRDLHPPKAAWDAE
TPANMLEPVGLPNVDIKWNRHCVLGTTGVELLDGLPPVLEYDFQVNKGMDPDAHPYGIFFHDVADTKTTGTNEFLKFNHI
DTVVVGGLALDFCVKKSVTQALELGFKVIVNLASSRAVLPDTIDAVIAEMEEKGAVFVKNADDIIVENFA
>Mature_229_residues
ANASIETDKESAMTVLVNYQTTAVIDVDPEKGFTELCPDELPVTGALEIVPELLKNHTKGRLKLVSRDLHPPKAAWDAET
PANMLEPVGLPNVDIKWNRHCVLGTTGVELLDGLPPVLEYDFQVNKGMDPDAHPYGIFFHDVADTKTTGTNEFLKFNHID
TVVVGGLALDFCVKKSVTQALELGFKVIVNLASSRAVLPDTIDAVIAEMEEKGAVFVKNADDIIVENFA

Specific function: NAD biosynthesis; nicotinamide to NaMN; first step. [C]

COG id: COG1335

COG function: function code Q; Amidases related to nicotinamidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI87081970, Length=174, Percent_Identity=31.0344827586207, Blast_Score=65, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6321401, Length=162, Percent_Identity=32.0987654320988, Blast_Score=68, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 3.5.1.19

Molecular weight: Translated: 25069; Mature: 24938

Theoretical pI: Translated: 4.36; Mature: 4.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANASIETDKESAMTVLVNYQTTAVIDVDPEKGFTELCPDELPVTGALEIVPELLKNHTK
CCCCCCCCCCCCEEEEEEEECEEEEEEECCCCCHHHHCCCCCCCCHHHHHHHHHHHCCCC
GRLKLVSRDLHPPKAAWDAETPANMLEPVGLPNVDIKWNRHCVLGTTGVELLDGLPPVLE
CEEEEEECCCCCCCCCCCCCCCHHHHCCCCCCCCCEEECCEEEEECCHHHHHCCCCCHHE
YDFQVNKGMDPDAHPYGIFFHDVADTKTTGTNEFLKFNHIDTVVVGGLALDFCVKKSVTQ
ECEEECCCCCCCCCEEEEEEEECCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHH
ALELGFKVIVNLASSRAVLPDTIDAVIAEMEEKGAVFVKNADDIIVENFA
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCEEEEECCCCEEEECCC
>Mature Secondary Structure 
ANASIETDKESAMTVLVNYQTTAVIDVDPEKGFTELCPDELPVTGALEIVPELLKNHTK
CCCCCCCCCCCEEEEEEEECEEEEEEECCCCCHHHHCCCCCCCCHHHHHHHHHHHCCCC
GRLKLVSRDLHPPKAAWDAETPANMLEPVGLPNVDIKWNRHCVLGTTGVELLDGLPPVLE
CEEEEEECCCCCCCCCCCCCCCHHHHCCCCCCCCCEEECCEEEEECCHHHHHCCCCCHHE
YDFQVNKGMDPDAHPYGIFFHDVADTKTTGTNEFLKFNHIDTVVVGGLALDFCVKKSVTQ
ECEEECCCCCCCCCEEEEEEEECCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHH
ALELGFKVIVNLASSRAVLPDTIDAVIAEMEEKGAVFVKNADDIIVENFA
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCEEEEECCCCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: nicotinamide; H2O

Specific reaction: nicotinamide + H2O = nicotinate + NH3

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA