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The map label for this gene is gcvA [H]

Identifier: 156977780

GI number: 156977780

Start: 1717555

End: 1718439

Strand: Reverse

Name: gcvA [H]

Synonym: VIBHAR_06568

Alternate gene names: 156977780

Gene position: 1718439-1717555 (Counterclockwise)

Preceding gene: 156977783

Following gene: 156977778

Centisome position: 77.97

GC content: 46.89

Gene sequence:

>885_bases
ATGAAACTACCACCTCTTAGAGCGGTCCACTGTTTCGAATCCGTTGCGCGCAATTTGAGTTTTTCCCTTGCAGCAGAAGA
GCTCAACGTGACCCAAAGTGCGGTGAGCCATCAGATCCGTATATTAGAAGACTACCTTGGTGAAGCCTTGTTTGTCCGTC
AGGGGCGTAAGTTATCACTCTTGGATACTGGCTTTCGTTATCTTGAAGATATCAGCCCTGCCATTAGTGCGATTGCGATG
GCGAGTCAAAAGGTGCGTGAAGGAGAAAAGGGCAGTCTACGGCTAGCGATTTACAGCTCGCTGGCGGTGAAATGGCTGAT
CCCAAGATTGGCGGACTTTAAGCGTCAGTACCCAGAAATCGATCTCTCGCTCAATATGGTCGCCGGAGATCCCGAACAAA
CCGATAGTGTTGGTGATTGCTTTATTACGGTGCAAAAGCCGAAGCGTAATTATATGGCAGTACGCTTATATGATGAGATT
TTGTATCCAGTATGCAGCCTCAATATCTGGAAAGAAATCCAAGACCAACCTATGCCGAATGCGTTCTGGCACTATCCGAT
CCTTTCTACTGATTCTATCTATCGCGAACGAGGTAAAGATTGGGCAGAGTGGTGCAAAACCGGTGGGTTTGAACTGCCAA
AAGACATTGATATGCAGCACTTCAGTCACATGCTGCTTGCTATCGAAGCCGCTCGTTACGACCAAGGCATTACGTTCGCT
AATGACTTCATGTTGAATGAACGAGATAAAGCTCAGGATTTGGTCTATATCCCTTCGCATGGACTGGAAACTGGAGACAG
CTTCTACTTCGTACACAAGAAGAATCGTGCTCGTCAGCCTGAAATCATCAAGTTGACTAACTGGCTTAAGCAACAGTGCT
TGTAG

Upstream 100 bases:

>100_bases
TTTCACTTTATTCCTCTCCTCATTCTTTGCCAAAGAGTAGGTGATGTCATAGGATGAAGTGAAATGATATTTATTGGTTT
AAGTATGAGATTTTTAGATA

Downstream 100 bases:

>100_bases
TGTAATTGGAGTGATTGCTTCGCTGTTCTCTCACGAAAATGAAAAAATCCGAGCAGGGGAGGCTCGGATTGGGTCTTTAT
CAGGATGAGATGAAATCGCT

Product: transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 294; Mature: 294

Protein sequence:

>294_residues
MKLPPLRAVHCFESVARNLSFSLAAEELNVTQSAVSHQIRILEDYLGEALFVRQGRKLSLLDTGFRYLEDISPAISAIAM
ASQKVREGEKGSLRLAIYSSLAVKWLIPRLADFKRQYPEIDLSLNMVAGDPEQTDSVGDCFITVQKPKRNYMAVRLYDEI
LYPVCSLNIWKEIQDQPMPNAFWHYPILSTDSIYRERGKDWAEWCKTGGFELPKDIDMQHFSHMLLAIEAARYDQGITFA
NDFMLNERDKAQDLVYIPSHGLETGDSFYFVHKKNRARQPEIIKLTNWLKQQCL

Sequences:

>Translated_294_residues
MKLPPLRAVHCFESVARNLSFSLAAEELNVTQSAVSHQIRILEDYLGEALFVRQGRKLSLLDTGFRYLEDISPAISAIAM
ASQKVREGEKGSLRLAIYSSLAVKWLIPRLADFKRQYPEIDLSLNMVAGDPEQTDSVGDCFITVQKPKRNYMAVRLYDEI
LYPVCSLNIWKEIQDQPMPNAFWHYPILSTDSIYRERGKDWAEWCKTGGFELPKDIDMQHFSHMLLAIEAARYDQGITFA
NDFMLNERDKAQDLVYIPSHGLETGDSFYFVHKKNRARQPEIIKLTNWLKQQCL
>Mature_294_residues
MKLPPLRAVHCFESVARNLSFSLAAEELNVTQSAVSHQIRILEDYLGEALFVRQGRKLSLLDTGFRYLEDISPAISAIAM
ASQKVREGEKGSLRLAIYSSLAVKWLIPRLADFKRQYPEIDLSLNMVAGDPEQTDSVGDCFITVQKPKRNYMAVRLYDEI
LYPVCSLNIWKEIQDQPMPNAFWHYPILSTDSIYRERGKDWAEWCKTGGFELPKDIDMQHFSHMLLAIEAARYDQGITFA
NDFMLNERDKAQDLVYIPSHGLETGDSFYFVHKKNRARQPEIIKLTNWLKQQCL

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=295, Percent_Identity=33.5593220338983, Blast_Score=152, Evalue=4e-38,
Organism=Escherichia coli, GI1788706, Length=295, Percent_Identity=29.4915254237288, Blast_Score=99, Evalue=5e-22,
Organism=Escherichia coli, GI1786448, Length=256, Percent_Identity=29.296875, Blast_Score=94, Evalue=9e-21,
Organism=Escherichia coli, GI157672245, Length=152, Percent_Identity=34.2105263157895, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1786401, Length=116, Percent_Identity=36.2068965517241, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1790262, Length=121, Percent_Identity=33.0578512396694, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1787128, Length=117, Percent_Identity=35.042735042735, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1787879, Length=121, Percent_Identity=33.0578512396694, Blast_Score=65, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33885; Mature: 33885

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLPPLRAVHCFESVARNLSFSLAAEELNVTQSAVSHQIRILEDYLGEALFVRQGRKLSL
CCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHCCCEEEH
LDTGFRYLEDISPAISAIAMASQKVREGEKGSLRLAIYSSLAVKWLIPRLADFKRQYPEI
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHCCCC
DLSLNMVAGDPEQTDSVGDCFITVQKPKRNYMAVRLYDEILYPVCSLNIWKEIQDQPMPN
CEEEEEEECCCCCCCCCCCEEEEEECCCCCEEEEEHHHHHHHHHHCCHHHHHHHCCCCCC
AFWHYPILSTDSIYRERGKDWAEWCKTGGFELPKDIDMQHFSHMLLAIEAARYDQGITFA
CEEECCCCCCHHHHHHHCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEH
NDFMLNERDKAQDLVYIPSHGLETGDSFYFVHKKNRARQPEIIKLTNWLKQQCL
HHHEECCCCCCCCEEEECCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MKLPPLRAVHCFESVARNLSFSLAAEELNVTQSAVSHQIRILEDYLGEALFVRQGRKLSL
CCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHCCCEEEH
LDTGFRYLEDISPAISAIAMASQKVREGEKGSLRLAIYSSLAVKWLIPRLADFKRQYPEI
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHCCCC
DLSLNMVAGDPEQTDSVGDCFITVQKPKRNYMAVRLYDEILYPVCSLNIWKEIQDQPMPN
CEEEEEEECCCCCCCCCCCEEEEEECCCCCEEEEEHHHHHHHHHHCCHHHHHHHCCCCCC
AFWHYPILSTDSIYRERGKDWAEWCKTGGFELPKDIDMQHFSHMLLAIEAARYDQGITFA
CEEECCCCCCHHHHHHHCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEH
NDFMLNERDKAQDLVYIPSHGLETGDSFYFVHKKNRARQPEIIKLTNWLKQQCL
HHHEECCCCCCCCEEEECCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]