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The map label for this gene is proW [H]

Identifier: 156977775

GI number: 156977775

Start: 1712998

End: 1713897

Strand: Direct

Name: proW [H]

Synonym: VIBHAR_06563

Alternate gene names: 156977775

Gene position: 1712998-1713897 (Clockwise)

Preceding gene: 156977774

Following gene: 156977776

Centisome position: 77.72

GC content: 48.56

Gene sequence:

>900_bases
ATGTCTGACTCGAATTGGCTTAGCGAATTCCCAGAAATGGATCGCTCAGATCTCAGAGCCATCCGCAAAACCTTAGATGG
AGCCTACCGCGACTTCTCTCGCGAGTACGGCGACCTTATTGAATCATTCTTCGACCCTCTTCTCTCATTTCTCGTTTGGT
TCGAAAAACTCCTCATTTCTACACCATGGATGATCATTCTTGGTGTGTGTACTGCGTTGGTTTATGCCGCTAGTCGCTCA
TGGAAACTGGCTGTCGCGTGTTTCGTGTCATTGATCCTGATAGGGTACTTTGGCATGTGGGAAGACACGATGCGGACACT
CAGCATCATCACGGTGTGTACCATGCTCGCCATTGCGCTCGGCATTCCAATTGGTATTGCCATGGCGCGATCAAATCGTG
TGCAATCCGTCGTGACACCACTGCTCGACGTGATGCAAACCATGCCTGCGTTCGTCTATCTGATTCCTGTCGTGATGCTC
CTTGGTATCGGTAAGATCCCTGGCTTGATTGCGGTTGTTATCTACGCAATCCCACCAGTAATCCGTTTGACCAACTTAGG
CATCCGACTCGTAGACAAAGAAGTACTTGAAGCCGCCACTGCCTTTGGTGCAAGTAACAAGCAACGCTTAGTCGGCGTAC
AATTACCTTTAGCAATGCCAACCATTATGGCTGGTATCAACCAAACCATCATGATGGCACTTTCTATGGTTGTTATCGCC
TCTATGATTGGCGTTAAAGGCTTAGGTCAGCCAGTATTAAAATCAATCACCAACCAGTACTTCACTCTTGGTCTGCTTAA
TGGCCTAGCGATTGTGGCACTGGCGATTCTGTTTGACCGTGCGTCGCAAGCTTACGCGAAGCGAACCCAAGCACACTTAG
GAGATCTGAAACATGACTAA

Upstream 100 bases:

>100_bases
GGTGCACTGTAAGACTTAGACGTTTCGTAAATGGTATAGGAATACCAAAATTGTTGTATGCCGAGGCTCGCTTGAGCCTC
GGCAAGAAAAGGAAGTAAAC

Downstream 100 bases:

>100_bases
GCCACTTATTGAAATCAGCGGGCTGTACAAGATCTTCGGACCAAAGCCAAAAACAGTCATCGAGCGAGTAAAACAAGGTC
AGAGCAAAGACCAAATTCTG

Product: glycine betaine/L-proline ABC transporter permease protein

Products: ADP; phosphate; L-proline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 299; Mature: 298

Protein sequence:

>299_residues
MSDSNWLSEFPEMDRSDLRAIRKTLDGAYRDFSREYGDLIESFFDPLLSFLVWFEKLLISTPWMIILGVCTALVYAASRS
WKLAVACFVSLILIGYFGMWEDTMRTLSIITVCTMLAIALGIPIGIAMARSNRVQSVVTPLLDVMQTMPAFVYLIPVVML
LGIGKIPGLIAVVIYAIPPVIRLTNLGIRLVDKEVLEAATAFGASNKQRLVGVQLPLAMPTIMAGINQTIMMALSMVVIA
SMIGVKGLGQPVLKSITNQYFTLGLLNGLAIVALAILFDRASQAYAKRTQAHLGDLKHD

Sequences:

>Translated_299_residues
MSDSNWLSEFPEMDRSDLRAIRKTLDGAYRDFSREYGDLIESFFDPLLSFLVWFEKLLISTPWMIILGVCTALVYAASRS
WKLAVACFVSLILIGYFGMWEDTMRTLSIITVCTMLAIALGIPIGIAMARSNRVQSVVTPLLDVMQTMPAFVYLIPVVML
LGIGKIPGLIAVVIYAIPPVIRLTNLGIRLVDKEVLEAATAFGASNKQRLVGVQLPLAMPTIMAGINQTIMMALSMVVIA
SMIGVKGLGQPVLKSITNQYFTLGLLNGLAIVALAILFDRASQAYAKRTQAHLGDLKHD
>Mature_298_residues
SDSNWLSEFPEMDRSDLRAIRKTLDGAYRDFSREYGDLIESFFDPLLSFLVWFEKLLISTPWMIILGVCTALVYAASRSW
KLAVACFVSLILIGYFGMWEDTMRTLSIITVCTMLAIALGIPIGIAMARSNRVQSVVTPLLDVMQTMPAFVYLIPVVMLL
GIGKIPGLIAVVIYAIPPVIRLTNLGIRLVDKEVLEAATAFGASNKQRLVGVQLPLAMPTIMAGINQTIMMALSMVVIAS
MIGVKGLGQPVLKSITNQYFTLGLLNGLAIVALAILFDRASQAYAKRTQAHLGDLKHD

Specific function: Involved in a multicomponent binding-protein-dependent transport system for glycine betaine/L-proline [H]

COG id: COG4176

COG function: function code E; ABC-type proline/glycine betaine transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1789033, Length=305, Percent_Identity=42.6229508196721, Blast_Score=213, Evalue=9e-57,
Organism=Escherichia coli, GI1788451, Length=191, Percent_Identity=32.9842931937173, Blast_Score=95, Evalue=6e-21,
Organism=Escherichia coli, GI1788449, Length=189, Percent_Identity=30.6878306878307, Blast_Score=88, Evalue=7e-19,
Organism=Escherichia coli, GI1786564, Length=152, Percent_Identity=30.2631578947368, Blast_Score=71, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 32790; Mature: 32659

Theoretical pI: Translated: 8.77; Mature: 8.77

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
5.4 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
5.0 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDSNWLSEFPEMDRSDLRAIRKTLDGAYRDFSREYGDLIESFFDPLLSFLVWFEKLLIS
CCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TPWMIILGVCTALVYAASRSWKLAVACFVSLILIGYFGMWEDTMRTLSIITVCTMLAIAL
CHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GIPIGIAMARSNRVQSVVTPLLDVMQTMPAFVYLIPVVMLLGIGKIPGLIAVVIYAIPPV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
IRLTNLGIRLVDKEVLEAATAFGASNKQRLVGVQLPLAMPTIMAGINQTIMMALSMVVIA
HHHHHCCHHHHHHHHHHHHHHHCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHH
SMIGVKGLGQPVLKSITNQYFTLGLLNGLAIVALAILFDRASQAYAKRTQAHLGDLKHD
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
SDSNWLSEFPEMDRSDLRAIRKTLDGAYRDFSREYGDLIESFFDPLLSFLVWFEKLLIS
CCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TPWMIILGVCTALVYAASRSWKLAVACFVSLILIGYFGMWEDTMRTLSIITVCTMLAIAL
CHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GIPIGIAMARSNRVQSVVTPLLDVMQTMPAFVYLIPVVMLLGIGKIPGLIAVVIYAIPPV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
IRLTNLGIRLVDKEVLEAATAFGASNKQRLVGVQLPLAMPTIMAGINQTIMMALSMVVIA
HHHHHCCHHHHHHHHHHHHHHHCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHH
SMIGVKGLGQPVLKSITNQYFTLGLLNGLAIVALAILFDRASQAYAKRTQAHLGDLKHD
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-proline [Periplasm]; H2O [C]

Specific reaction: ATP + L-proline [Periplasm] + H2O = ADP + phosphate + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2649479; 9205837; 9278503 [H]