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The map label for this gene is ycjG [H]

Identifier: 156977770

GI number: 156977770

Start: 1709321

End: 1710307

Strand: Direct

Name: ycjG [H]

Synonym: VIBHAR_06558

Alternate gene names: 156977770

Gene position: 1709321-1710307 (Clockwise)

Preceding gene: 156977769

Following gene: 156977771

Centisome position: 77.55

GC content: 49.54

Gene sequence:

>987_bases
ATGAAAATTCATGTAGAGCCTGTCACCATTGCGATGGCGACGCCTTTCCGTATATCACGTGGCAGCCGAACCGAATGTCA
CGTGGTGCGAGTAACCATAGAGCAGAACGGCATCAGCGCCCAAGGTGAATGTACGCCTTATCCCCGTTATGGCGAGTCGG
TGGAATCGGTGATCAAAGCCATTTCTCAAGCCGCTCAGGAACTACAAGGTTTGTTTGCAAAAGGCGATGCGAACATAGTC
TCTCTAAGAGAAAAGTTGCAAACCCTTCTCCCTGGCGGTGCGGCACGTAATGCACTCGACTGTGCGATGTGGAACTTACA
AGCTAAGCTAGCGGGCAAGCAACACACTCAAGATTTATTTGAACTGCCAACATCGATTGTCACTGCGATGACCGTGTCGA
TTAACACGCCAGAGGCAATGGCAAACCAGACCCGTAGTTACATCGAACAAGGCGCAAAACTACTTAAAGTGAAGCTCGAT
GGCGAACAAGTGATTGAACGTGTTCGCGCAGTTCGTGAAGCGGCAGGTGAGGCAATGATCGTGCTGGATGCAAACGAAGC
ATGGCAAGAACTGGATTTAGACGCCACCTTTGCCGCGCTAGTACCACTGAATATTGCGATGATTGAGCAGCCTTTGCCAT
CGAATGACGATGACAAGCTAAAAGACATTTCGCACCCAATTCCACTTTGTGCCGATGAAAGTTGCCATACACGCCAGCAA
TTGCATGAATTAGTGGGCAAATACGATATGGTCAACATCAAGCTCGACAAGACGGGTGGTCTTACCGAAGCCCTACTGCT
TGCAGAAGAAGCGAAACGCCTTGGCTTTACCTTGATGTCTGGTTGCATGTTAGGCACTTCACTGGCGATGCGTGCAGCGC
TACCTATTGCTGTCCAATCGAAAGTGGTCGACTTAGATGGTCCGGTTTTACTGGGTCAGGATGTTGAGCCTGCTTTGGTA
TATCGAGAAGGTGAAATCGTCCTGTAA

Upstream 100 bases:

>100_bases
ACTGGACTGAGAAATACCAAGTACCTGTCACCGATCCTGTGCGTTTTGGCGTAGAGCAAATTGCTCAAAAGATTATTGCG
ATTTAAGGATTGTAGACGCG

Downstream 100 bases:

>100_bases
AGGATCAGTGATCACCCCATCACCCATTTCATGACCTAGATTAAAAAACAATCACCGCCAGCATGAAATAAGTCACATAA
ATTACGGGGTTATTACGGCA

Product: muconate cycloisomerase I

Products: NA

Alternate protein names: AE epimerase; AEE [H]

Number of amino acids: Translated: 328; Mature: 328

Protein sequence:

>328_residues
MKIHVEPVTIAMATPFRISRGSRTECHVVRVTIEQNGISAQGECTPYPRYGESVESVIKAISQAAQELQGLFAKGDANIV
SLREKLQTLLPGGAARNALDCAMWNLQAKLAGKQHTQDLFELPTSIVTAMTVSINTPEAMANQTRSYIEQGAKLLKVKLD
GEQVIERVRAVREAAGEAMIVLDANEAWQELDLDATFAALVPLNIAMIEQPLPSNDDDKLKDISHPIPLCADESCHTRQQ
LHELVGKYDMVNIKLDKTGGLTEALLLAEEAKRLGFTLMSGCMLGTSLAMRAALPIAVQSKVVDLDGPVLLGQDVEPALV
YREGEIVL

Sequences:

>Translated_328_residues
MKIHVEPVTIAMATPFRISRGSRTECHVVRVTIEQNGISAQGECTPYPRYGESVESVIKAISQAAQELQGLFAKGDANIV
SLREKLQTLLPGGAARNALDCAMWNLQAKLAGKQHTQDLFELPTSIVTAMTVSINTPEAMANQTRSYIEQGAKLLKVKLD
GEQVIERVRAVREAAGEAMIVLDANEAWQELDLDATFAALVPLNIAMIEQPLPSNDDDKLKDISHPIPLCADESCHTRQQ
LHELVGKYDMVNIKLDKTGGLTEALLLAEEAKRLGFTLMSGCMLGTSLAMRAALPIAVQSKVVDLDGPVLLGQDVEPALV
YREGEIVL
>Mature_328_residues
MKIHVEPVTIAMATPFRISRGSRTECHVVRVTIEQNGISAQGECTPYPRYGESVESVIKAISQAAQELQGLFAKGDANIV
SLREKLQTLLPGGAARNALDCAMWNLQAKLAGKQHTQDLFELPTSIVTAMTVSINTPEAMANQTRSYIEQGAKLLKVKLD
GEQVIERVRAVREAAGEAMIVLDANEAWQELDLDATFAALVPLNIAMIEQPLPSNDDDKLKDISHPIPLCADESCHTRQQ
LHELVGKYDMVNIKLDKTGGLTEALLLAEEAKRLGFTLMSGCMLGTSLAMRAALPIAVQSKVVDLDGPVLLGQDVEPALV
YREGEIVL

Specific function: Catalyzes the epimerization of L-Ala-D-Glu to L-Ala-L- Glu and has a role in the recycling of the murein peptide, of which L-Ala-D-Glu is a component. Is also able to catalyze the reverse reaction and the epimerization of all the L-Ala-X dipeptides, excep

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI87081876, Length=317, Percent_Identity=53.3123028391167, Blast_Score=310, Evalue=7e-86,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018110
- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: NA

Molecular weight: Translated: 35635; Mature: 35635

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: PS00909 MR_MLE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIHVEPVTIAMATPFRISRGSRTECHVVRVTIEQNGISAQGECTPYPRYGESVESVIKA
CEEEEEEEEEEEECCEEECCCCCCEEEEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHH
ISQAAQELQGLFAKGDANIVSLREKLQTLLPGGAARNALDCAMWNLQAKLAGKQHTQDLF
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHH
ELPTSIVTAMTVSINTPEAMANQTRSYIEQGAKLLKVKLDGEQVIERVRAVREAAGEAMI
HHHHHHHHEEEEECCCCHHHHHHHHHHHHCCCEEEEEEECHHHHHHHHHHHHHHCCCEEE
VLDANEAWQELDLDATFAALVPLNIAMIEQPLPSNDDDKLKDISHPIPLCADESCHTRQQ
EEECCHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCCCCCCCHHHHHH
LHELVGKYDMVNIKLDKTGGLTEALLLAEEAKRLGFTLMSGCMLGTSLAMRAALPIAVQS
HHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHC
KVVDLDGPVLLGQDVEPALVYREGEIVL
EEEECCCCEEECCCCCCEEEEECCCEEC
>Mature Secondary Structure
MKIHVEPVTIAMATPFRISRGSRTECHVVRVTIEQNGISAQGECTPYPRYGESVESVIKA
CEEEEEEEEEEEECCEEECCCCCCEEEEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHH
ISQAAQELQGLFAKGDANIVSLREKLQTLLPGGAARNALDCAMWNLQAKLAGKQHTQDLF
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHH
ELPTSIVTAMTVSINTPEAMANQTRSYIEQGAKLLKVKLDGEQVIERVRAVREAAGEAMI
HHHHHHHHEEEEECCCCHHHHHHHHHHHHCCCEEEEEEECHHHHHHHHHHHHHHCCCEEE
VLDANEAWQELDLDATFAALVPLNIAMIEQPLPSNDDDKLKDISHPIPLCADESCHTRQQ
EEECCHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCCCCCCCHHHHHH
LHELVGKYDMVNIKLDKTGGLTEALLLAEEAKRLGFTLMSGCMLGTSLAMRAALPIAVQS
HHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHC
KVVDLDGPVLLGQDVEPALVYREGEIVL
EEEECCCCEEECCCCCCEEEEECCCEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7499381; 9097039; 9278503 [H]