Click here to switch to the map view.

The map label for this gene is tyrS1 [H]

Identifier: 156977732

GI number: 156977732

Start: 1676850

End: 1678106

Strand: Direct

Name: tyrS1 [H]

Synonym: VIBHAR_06520

Alternate gene names: 156977732

Gene position: 1676850-1678106 (Clockwise)

Preceding gene: 156977731

Following gene: 156977733

Centisome position: 76.08

GC content: 48.45

Gene sequence:

>1257_bases
ATGACACTGATTGAAGATTTACAAGCTCGCGGTTTAGTTGCCCAAGCGAGCGATTTAGACCAAGTTCAACAACTGCTATC
TGAGCCACAAACCTTGTACTGCGGTTTCGATCCAACCGCAGGTAGCCTGCACATTGGCCACCTTGTGCCACTGATTATGC
TCAAACGTTTTCAAGATGCAGGACACCAAGCCATCGCCCTAATTGGCGGTGCGACCGGTATGATTGGTGACCCGAGCTTT
AAAGCCACAGAGCGAAGCTTAAACTCAGAAGAAACCGTAACGGGCTGGGTACAAGATCTGTCGAATCAAATTCAACAGTT
GATGAATAACCAAGTCAGCAAACCAATGATCATGGTAAACAACGCGGATTGGGTGAAGCAGATTAACGTGATCGACTTCT
TCCGCGACGTAGGTAAGCATTTCTCCGTGAATACCATGATCAGCCGAGAGTCGGTGAAGCAACGCTTACAACGTCCAGAT
CAAGGCATCTCATTTACAGAGTTTAGTTACGCGCTACTGCAATCTTACGACTTCGCAGAATTAAACCGTCAGTACGGCTG
CCGCCTACAAATCGGTGGCAATGACCAATGGGGCAACAGTGTTAGCGGCATTGACCTTACTCGCCGTCAGAATGGTGAGC
AGGTCTTCGGTCTAACCCTACCTTTGATCACCAAATCTGACGGCACTAAGTTTGGTAAAACCGAAGGTGGCGCTGTGTGG
TTAGATCCAAGTAAGACCTCGCCTTACGCCTTCTACCAATTCTGGTTATCCGCGGAAGATGCCGATGTCTATCACTTCCT
GCGCTACTACACCTTCTTGAGTTGCGATGAGATTGCCGCGATTGAAGCACAAGACAAAGAAAGCAAAGGCAAACCGCAAG
CACAGCGTATCTTAGCAGAACAAATGACGCGTTTTGTCCACGGTGAAGAGGGCTTAGCAAGTGCAGAGCGAATCACTCAA
GCGCTATTTAGCGGCAATGTGCAGCAACTCAGTTTGAGCGAGCTAAAACAGTTAGAACTCGATGGCTTGCCAAGCATGCA
AAGTGCTGAGCAAGACTTGGTAGAACTACTGGTTGAATCGGGCTTAGCCAACTCAAAGCGCATTGCTCGTGAGCTGATTG
GCAACAACGCCATCAGTGTGAGCGGAGAGAAAGTGGATGCACAAACCCCAAGTTTGAACTTCCCGTTGTTCGATCAATAC
TGGTTGCTTCAACGCGGTAAAAAGCATTTCTACTTGGTGAAACGCATCGCGGGTTAA

Upstream 100 bases:

>100_bases
GATCACTCAATCAAGATAACAAACTGTCCCTTTAGGGATATATCTCGATTCCGCATCGCCTTTTAATCTAAATACAACCA
AACCAGATAAAGATAAGACG

Downstream 100 bases:

>100_bases
CGGTTCCCATCTAACGACCTACGACCTAGCTAGACCAACTAAAACGCGAGCCAAAGTGCTCGCGTTTTTGTTTCCCTATT
TTGTGAAGCCAAAAGCTAAC

Product: tyrosyl-tRNA synthetase

Products: NA

Alternate protein names: Tyrosine--tRNA ligase 1; TyrRS 1 [H]

Number of amino acids: Translated: 418; Mature: 417

Protein sequence:

>418_residues
MTLIEDLQARGLVAQASDLDQVQQLLSEPQTLYCGFDPTAGSLHIGHLVPLIMLKRFQDAGHQAIALIGGATGMIGDPSF
KATERSLNSEETVTGWVQDLSNQIQQLMNNQVSKPMIMVNNADWVKQINVIDFFRDVGKHFSVNTMISRESVKQRLQRPD
QGISFTEFSYALLQSYDFAELNRQYGCRLQIGGNDQWGNSVSGIDLTRRQNGEQVFGLTLPLITKSDGTKFGKTEGGAVW
LDPSKTSPYAFYQFWLSAEDADVYHFLRYYTFLSCDEIAAIEAQDKESKGKPQAQRILAEQMTRFVHGEEGLASAERITQ
ALFSGNVQQLSLSELKQLELDGLPSMQSAEQDLVELLVESGLANSKRIARELIGNNAISVSGEKVDAQTPSLNFPLFDQY
WLLQRGKKHFYLVKRIAG

Sequences:

>Translated_418_residues
MTLIEDLQARGLVAQASDLDQVQQLLSEPQTLYCGFDPTAGSLHIGHLVPLIMLKRFQDAGHQAIALIGGATGMIGDPSF
KATERSLNSEETVTGWVQDLSNQIQQLMNNQVSKPMIMVNNADWVKQINVIDFFRDVGKHFSVNTMISRESVKQRLQRPD
QGISFTEFSYALLQSYDFAELNRQYGCRLQIGGNDQWGNSVSGIDLTRRQNGEQVFGLTLPLITKSDGTKFGKTEGGAVW
LDPSKTSPYAFYQFWLSAEDADVYHFLRYYTFLSCDEIAAIEAQDKESKGKPQAQRILAEQMTRFVHGEEGLASAERITQ
ALFSGNVQQLSLSELKQLELDGLPSMQSAEQDLVELLVESGLANSKRIARELIGNNAISVSGEKVDAQTPSLNFPLFDQY
WLLQRGKKHFYLVKRIAG
>Mature_417_residues
TLIEDLQARGLVAQASDLDQVQQLLSEPQTLYCGFDPTAGSLHIGHLVPLIMLKRFQDAGHQAIALIGGATGMIGDPSFK
ATERSLNSEETVTGWVQDLSNQIQQLMNNQVSKPMIMVNNADWVKQINVIDFFRDVGKHFSVNTMISRESVKQRLQRPDQ
GISFTEFSYALLQSYDFAELNRQYGCRLQIGGNDQWGNSVSGIDLTRRQNGEQVFGLTLPLITKSDGTKFGKTEGGAVWL
DPSKTSPYAFYQFWLSAEDADVYHFLRYYTFLSCDEIAAIEAQDKESKGKPQAQRILAEQMTRFVHGEEGLASAERITQA
LFSGNVQQLSLSELKQLELDGLPSMQSAEQDLVELLVESGLANSKRIARELIGNNAISVSGEKVDAQTPSLNFPLFDQYW
LLQRGKKHFYLVKRIAG

Specific function: Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction:tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr) [H]

COG id: COG0162

COG function: function code J; Tyrosyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI94681057, Length=416, Percent_Identity=37.7403846153846, Blast_Score=256, Evalue=3e-68,
Organism=Escherichia coli, GI1787925, Length=419, Percent_Identity=58.472553699284, Blast_Score=496, Evalue=1e-141,
Organism=Caenorhabditis elegans, GI25145802, Length=400, Percent_Identity=36.5, Blast_Score=224, Evalue=5e-59,
Organism=Caenorhabditis elegans, GI25145805, Length=302, Percent_Identity=34.7682119205298, Blast_Score=165, Evalue=3e-41,
Organism=Saccharomyces cerevisiae, GI6325160, Length=441, Percent_Identity=34.6938775510204, Blast_Score=250, Evalue=3e-67,
Organism=Drosophila melanogaster, GI20130383, Length=415, Percent_Identity=39.2771084337349, Blast_Score=293, Evalue=2e-79,

Paralogues:

None

Copy number: 892 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002942
- InterPro:   IPR002307 [H]

Pfam domain/function: PF00579 tRNA-synt_1b [H]

EC number: =6.1.1.1 [H]

Molecular weight: Translated: 46671; Mature: 46540

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: PS50889 S4 ; PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLIEDLQARGLVAQASDLDQVQQLLSEPQTLYCGFDPTAGSLHIGHLVPLIMLKRFQDA
CCCHHHHHHCCCHHHHCCHHHHHHHHCCCCEEEECCCCCCCCEEHHHHHHHHHHHHHHHC
GHQAIALIGGATGMIGDPSFKATERSLNSEETVTGWVQDLSNQIQQLMNNQVSKPMIMVN
CCEEEEEEECCCCCCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEE
NADWVKQINVIDFFRDVGKHFSVNTMISRESVKQRLQRPDQGISFTEFSYALLQSYDFAE
CCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCHHH
LNRQYGCRLQIGGNDQWGNSVSGIDLTRRQNGEQVFGLTLPLITKSDGTKFGKTEGGAVW
HHHCCCCEEEECCCCCCCCCCCCCEEHCCCCCCEEEEEEEEEEECCCCCCCCCCCCCEEE
LDPSKTSPYAFYQFWLSAEDADVYHFLRYYTFLSCDEIAAIEAQDKESKGKPQAQRILAE
ECCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHCCCHHHEECCCCCCCCCCHHHHHHHHH
QMTRFVHGEEGLASAERITQALFSGNVQQLSLSELKQLELDGLPSMQSAEQDLVELLVES
HHHHHHCCCCCHHHHHHHHHHHHCCCCCEECHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
GLANSKRIARELIGNNAISVSGEKVDAQTPSLNFPLFDQYWLLQRGKKHFYLVKRIAG
CCCCHHHHHHHHHCCCCEEECCCEECCCCCCCCCCCHHHHHHHHCCCCCEEHHHHHCC
>Mature Secondary Structure 
TLIEDLQARGLVAQASDLDQVQQLLSEPQTLYCGFDPTAGSLHIGHLVPLIMLKRFQDA
CCHHHHHHCCCHHHHCCHHHHHHHHCCCCEEEECCCCCCCCEEHHHHHHHHHHHHHHHC
GHQAIALIGGATGMIGDPSFKATERSLNSEETVTGWVQDLSNQIQQLMNNQVSKPMIMVN
CCEEEEEEECCCCCCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEE
NADWVKQINVIDFFRDVGKHFSVNTMISRESVKQRLQRPDQGISFTEFSYALLQSYDFAE
CCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCHHH
LNRQYGCRLQIGGNDQWGNSVSGIDLTRRQNGEQVFGLTLPLITKSDGTKFGKTEGGAVW
HHHCCCCEEEECCCCCCCCCCCCCEEHCCCCCCEEEEEEEEEEECCCCCCCCCCCCCEEE
LDPSKTSPYAFYQFWLSAEDADVYHFLRYYTFLSCDEIAAIEAQDKESKGKPQAQRILAE
ECCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHCCCHHHEECCCCCCCCCCHHHHHHHHH
QMTRFVHGEEGLASAERITQALFSGNVQQLSLSELKQLELDGLPSMQSAEQDLVELLVES
HHHHHHCCCCCHHHHHHHHHHHHCCCCCEECHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
GLANSKRIARELIGNNAISVSGEKVDAQTPSLNFPLFDQYWLLQRGKKHFYLVKRIAG
CCCCHHHHHHHHHCCCCEEECCCEECCCCCCCCCCCHHHHHHHHCCCCCEEHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12620739 [H]