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The map label for this gene is dacA [H]

Identifier: 156977638

GI number: 156977638

Start: 1586098

End: 1587267

Strand: Reverse

Name: dacA [H]

Synonym: VIBHAR_06426

Alternate gene names: 156977638

Gene position: 1587267-1586098 (Counterclockwise)

Preceding gene: 156977640

Following gene: 156977637

Centisome position: 72.02

GC content: 49.23

Gene sequence:

>1170_bases
ATGAGAAAAAACACTTTATTCAATGCTACGTTAGCGACCACCTTGACTGCTGTTTCTGCGTTTAGTTTTGCTGCTCCTAC
CGTTGTTCCTAATGCCCCAGAGCTGAGCTCTCGTGGCTATGTTTTAATGGATTACCACACTGGCACTGTGCTAGTTGAAC
GTGATGCCGACAAGCGCCTCAACCCGGCAAGTCTGACTAAGCTAATGACGGCTTATGTTGCGGGCCAAGAGGTTAAGTCA
GGTAACATCAGCCTAGACGATGAAGTGGTGATAAGCCGCAACGCCTGGGCGAAAAACTTCCCAGATTCGTCAAAGATGTT
TATCGAGGTAGGGACGTCGGTTTCACTTTCTGATCTTTACCGAGGTCTGATTGTTCAATCGGGTAACGATGCCAGTGTCG
CCATCGCAGAACATGTTGCGGGTAGTGAAGCTGGCTTTGTTGGTTTGATGAACAGTTGGGCGAACCAACTTGGTTTGTCT
AACTCTGCCTTTACCAACCCACATGGTTTGGACAGTGATGGTCTGTATTCAACCCCGCGCGACATCGCAACGCTAGGCCG
TGCGATCATTCGTGACCTGCCAGATATCTACCCGTTATACAGCGAAACGTCGTTTACTTATAACGGTATTACCCAATACA
ACCGTAACGGCTTGCTACGTGATCGCAGCATGAGTGTTGATGGCATGAAGACGGGTTACACCAGCGGCGCAGGTTACAGC
TTGGCGACTTCTGCAACCAACGGAGACATGCGTTTAGTCGCGGTTGTGATGGGCGCGAAAAGCCCGAGCGTACGTGAATC
AGAGAGTAAACAGCTTCTGAGCTACGGTTTCCGTTTCTTCGATACGGTTGTTCCGACACAAGCGGGTAAAGAAGTGGCAA
CGGCACGTGTTTGGATGGGTAACCAAGACGAATTAAAAGTGGGTGTTGACCGCGATATCTACCTAACACTACCAAAAGGT
GACGTGAACAAGCTGGAAGCGGAAGTGGAATACAACGGCGACCTGATGGCACCGATTCAACAAAATCAAGTAGTTGGTAA
GGTGCTGTACAAAGTTGACGGTGACGTGGTAAAAGAAGCGGAACTGGTTGCGCTAGAGCCTGTTGATGAAGGCGGTATCT
TTAAGCGTTTGATGGACTGGTTCAAACGTCTTGTGGCAAGCTGGTTCTAA

Upstream 100 bases:

>100_bases
TATCGAGTAAACGTAACCAATCGTAACAGAGGTGCTGTAACTGAACAGCGTTTCCATATACTCGACTTAGTTTGTAAGTT
GTAAGCCACAGAATGCTGCT

Downstream 100 bases:

>100_bases
CCAATACTCCCAATATTAAATTTACTAAGGCGGCCGAAATGGTCGCCTTTTTGTTTTGAGCACTCATCAAGAGAACCATT
CAGAACAAAAAAACGGAGTT

Product: D-alanyl-D-alanine carboxypeptidase

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein 5; PBP-5 [H]

Number of amino acids: Translated: 389; Mature: 389

Protein sequence:

>389_residues
MRKNTLFNATLATTLTAVSAFSFAAPTVVPNAPELSSRGYVLMDYHTGTVLVERDADKRLNPASLTKLMTAYVAGQEVKS
GNISLDDEVVISRNAWAKNFPDSSKMFIEVGTSVSLSDLYRGLIVQSGNDASVAIAEHVAGSEAGFVGLMNSWANQLGLS
NSAFTNPHGLDSDGLYSTPRDIATLGRAIIRDLPDIYPLYSETSFTYNGITQYNRNGLLRDRSMSVDGMKTGYTSGAGYS
LATSATNGDMRLVAVVMGAKSPSVRESESKQLLSYGFRFFDTVVPTQAGKEVATARVWMGNQDELKVGVDRDIYLTLPKG
DVNKLEAEVEYNGDLMAPIQQNQVVGKVLYKVDGDVVKEAELVALEPVDEGGIFKRLMDWFKRLVASWF

Sequences:

>Translated_389_residues
MRKNTLFNATLATTLTAVSAFSFAAPTVVPNAPELSSRGYVLMDYHTGTVLVERDADKRLNPASLTKLMTAYVAGQEVKS
GNISLDDEVVISRNAWAKNFPDSSKMFIEVGTSVSLSDLYRGLIVQSGNDASVAIAEHVAGSEAGFVGLMNSWANQLGLS
NSAFTNPHGLDSDGLYSTPRDIATLGRAIIRDLPDIYPLYSETSFTYNGITQYNRNGLLRDRSMSVDGMKTGYTSGAGYS
LATSATNGDMRLVAVVMGAKSPSVRESESKQLLSYGFRFFDTVVPTQAGKEVATARVWMGNQDELKVGVDRDIYLTLPKG
DVNKLEAEVEYNGDLMAPIQQNQVVGKVLYKVDGDVVKEAELVALEPVDEGGIFKRLMDWFKRLVASWF
>Mature_389_residues
MRKNTLFNATLATTLTAVSAFSFAAPTVVPNAPELSSRGYVLMDYHTGTVLVERDADKRLNPASLTKLMTAYVAGQEVKS
GNISLDDEVVISRNAWAKNFPDSSKMFIEVGTSVSLSDLYRGLIVQSGNDASVAIAEHVAGSEAGFVGLMNSWANQLGLS
NSAFTNPHGLDSDGLYSTPRDIATLGRAIIRDLPDIYPLYSETSFTYNGITQYNRNGLLRDRSMSVDGMKTGYTSGAGYS
LATSATNGDMRLVAVVMGAKSPSVRESESKQLLSYGFRFFDTVVPTQAGKEVATARVWMGNQDELKVGVDRDIYLTLPKG
DVNKLEAEVEYNGDLMAPIQQNQVVGKVLYKVDGDVVKEAELVALEPVDEGGIFKRLMDWFKRLVASWF

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein. Note=N-terminal lies in the periplasmic space (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI1786851, Length=401, Percent_Identity=47.8802992518703, Blast_Score=387, Evalue=1e-109,
Organism=Escherichia coli, GI1787062, Length=395, Percent_Identity=45.3164556962025, Blast_Score=355, Evalue=3e-99,
Organism=Escherichia coli, GI87082022, Length=388, Percent_Identity=44.5876288659794, Blast_Score=325, Evalue=2e-90,
Organism=Escherichia coli, GI87082054, Length=269, Percent_Identity=26.3940520446097, Blast_Score=74, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 42313; Mature: 42313

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKNTLFNATLATTLTAVSAFSFAAPTVVPNAPELSSRGYVLMDYHTGTVLVERDADKRL
CCCCEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCEEEEECCCCCCC
NPASLTKLMTAYVAGQEVKSGNISLDDEVVISRNAWAKNFPDSSKMFIEVGTSVSLSDLY
CHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCCCCCCCCEEEEEECCCCCHHHHH
RGLIVQSGNDASVAIAEHVAGSEAGFVGLMNSWANQLGLSNSAFTNPHGLDSDGLYSTPR
HHHEEECCCCCEEEEEHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCH
DIATLGRAIIRDLPDIYPLYSETSFTYNGITQYNRNGLLRDRSMSVDGMKTGYTSGAGYS
HHHHHHHHHHHHCCCCCCCCCCCCEEECCCEEECCCCCEECCCCCCCCCCCCCCCCCCCE
LATSATNGDMRLVAVVMGAKSPSVRESESKQLLSYGFRFFDTVVPTQAGKEVATARVWMG
EEECCCCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHEEEEEEEC
NQDELKVGVDRDIYLTLPKGDVNKLEAEVEYNGDLMAPIQQNQVVGKVLYKVDGDVVKEA
CCCCEEEECCCEEEEEECCCCCCCEEEEEEECCCEECCCHHHHHHHHHHHHCCCCHHCCC
ELVALEPVDEGGIFKRLMDWFKRLVASWF
EEEEECCCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRKNTLFNATLATTLTAVSAFSFAAPTVVPNAPELSSRGYVLMDYHTGTVLVERDADKRL
CCCCEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCEEEEECCCCCCC
NPASLTKLMTAYVAGQEVKSGNISLDDEVVISRNAWAKNFPDSSKMFIEVGTSVSLSDLY
CHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCCCCCCCCEEEEEECCCCCHHHHH
RGLIVQSGNDASVAIAEHVAGSEAGFVGLMNSWANQLGLSNSAFTNPHGLDSDGLYSTPR
HHHEEECCCCCEEEEEHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCH
DIATLGRAIIRDLPDIYPLYSETSFTYNGITQYNRNGLLRDRSMSVDGMKTGYTSGAGYS
HHHHHHHHHHHHCCCCCCCCCCCCEEECCCEEECCCCCEECCCCCCCCCCCCCCCCCCCE
LATSATNGDMRLVAVVMGAKSPSVRESESKQLLSYGFRFFDTVVPTQAGKEVATARVWMG
EEECCCCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHEEEEEEEC
NQDELKVGVDRDIYLTLPKGDVNKLEAEVEYNGDLMAPIQQNQVVGKVLYKVDGDVVKEA
CCCCEEEECCCEEEEEECCCCCCCEEEEEEECCCEECCCHHHHHHHHHHHHCCCCHHCCC
ELVALEPVDEGGIFKRLMDWFKRLVASWF
EEEEECCCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]