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The map label for this gene is insF1 [H]

Identifier: 156977460

GI number: 156977460

Start: 1427964

End: 1428815

Strand: Direct

Name: insF1 [H]

Synonym: VIBHAR_06247

Alternate gene names: 156977460

Gene position: 1427964-1428815 (Clockwise)

Preceding gene: 156977459

Following gene: 156977461

Centisome position: 64.79

GC content: 47.3

Gene sequence:

>852_bases
ATGCTCGAACACCAGCTGTGCTTCAGTGTTGTCCGCATAGCTAAGGTGTTCTGCGTTTCACGGAGTGGGTTTTATTACTG
GATTAAGCATCGCCACAAGGCCCTCCAGCGCGAGGTAACTCGCCAAGAGCTTGATACGAGGGTCAAAGAGGCTTTTGACA
ATAGCAAAGGCCGCGATGGTTCAAGGCGAATCCAGAAAGAGCTTGCTGAGAACGGTGATAGCCGTAATGTTAAAACCATT
GCCGCCAGTATGAAGCGGCAGGATTTAACACCGAAAGCGGCACGTAAGTTTAAGTGTACTACGGATAGCAAACATAAAAT
GCCAGTTGCTCCGAACCTGTTGGCACAGGATTTTAACGCGGAGGCTCCGAATCAAAAGTGGGCGGGAGACATCACCTACG
TTGCCACAAGCGAAGGCTGGCTGTACTTGGCGGTAATTATTGATCTTTACTCAAGACAAGTAGTCGGCTGGTCTATGGAT
ACCAGAATGACTGCTACGCTGGTCTGTGATGCGTTATCAATGGCCTTGTTCCGTCGCGGATTCCCTGAGCAAGTAATCGT
TCACAGTGATCGAGGTAGCCAGTATTGCTCAAAAGACTATCGAGACCTCATAACCGCTTATAATCTAAAGCAAAGTATGA
GTAGAAAAGGAAACTGCTGGGACAACGCTTGTGTGGAGAGCTTCTTCCATTCAATGAAAGTTGAAGCGCTCCAATATGAG
CCGATCATGATGCGAGACGAGATGCGCCAAGCGATTTTCGAATACATAGAGGTTGATTATAATCGGACGAGAAGGCACAG
TGCTCTTGGATATCTAAGCCCAGTTAACTTTGAACAACAAAATGTCGCTTAA

Upstream 100 bases:

>100_bases
AGCTCAAAAGACAACTAGCTGAGCAAGCTGAAGAGCTAGATATAGTAAAAAAGGCCGCCACCTACTTCGCGAAAAACCTA
AAGTAAATTGCTACGAATTT

Downstream 100 bases:

>100_bases
TGAAGTTTCCAGTCTGGCTGGAGCAGATCACTACCCTATCCTTCAATGGTGTGGAACAAGCATCAAACGGTTTGGAAAGT
ATTTCTAACGAAGCGATTTT

Product: integrase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 283; Mature: 283

Protein sequence:

>283_residues
MLEHQLCFSVVRIAKVFCVSRSGFYYWIKHRHKALQREVTRQELDTRVKEAFDNSKGRDGSRRIQKELAENGDSRNVKTI
AASMKRQDLTPKAARKFKCTTDSKHKMPVAPNLLAQDFNAEAPNQKWAGDITYVATSEGWLYLAVIIDLYSRQVVGWSMD
TRMTATLVCDALSMALFRRGFPEQVIVHSDRGSQYCSKDYRDLITAYNLKQSMSRKGNCWDNACVESFFHSMKVEALQYE
PIMMRDEMRQAIFEYIEVDYNRTRRHSALGYLSPVNFEQQNVA

Sequences:

>Translated_283_residues
MLEHQLCFSVVRIAKVFCVSRSGFYYWIKHRHKALQREVTRQELDTRVKEAFDNSKGRDGSRRIQKELAENGDSRNVKTI
AASMKRQDLTPKAARKFKCTTDSKHKMPVAPNLLAQDFNAEAPNQKWAGDITYVATSEGWLYLAVIIDLYSRQVVGWSMD
TRMTATLVCDALSMALFRRGFPEQVIVHSDRGSQYCSKDYRDLITAYNLKQSMSRKGNCWDNACVESFFHSMKVEALQYE
PIMMRDEMRQAIFEYIEVDYNRTRRHSALGYLSPVNFEQQNVA
>Mature_283_residues
MLEHQLCFSVVRIAKVFCVSRSGFYYWIKHRHKALQREVTRQELDTRVKEAFDNSKGRDGSRRIQKELAENGDSRNVKTI
AASMKRQDLTPKAARKFKCTTDSKHKMPVAPNLLAQDFNAEAPNQKWAGDITYVATSEGWLYLAVIIDLYSRQVVGWSMD
TRMTATLVCDALSMALFRRGFPEQVIVHSDRGSQYCSKDYRDLITAYNLKQSMSRKGNCWDNACVESFFHSMKVEALQYE
PIMMRDEMRQAIFEYIEVDYNRTRRHSALGYLSPVNFEQQNVA

Specific function: Involved in the transposition of the insertion sequence IS3 [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

Organism=Homo sapiens, GI310129419, Length=251, Percent_Identity=35.0597609561753, Blast_Score=149, Evalue=2e-36,
Organism=Escherichia coli, GI1788405, Length=281, Percent_Identity=55.5160142348754, Blast_Score=316, Evalue=9e-88,
Organism=Escherichia coli, GI1787263, Length=281, Percent_Identity=55.5160142348754, Blast_Score=316, Evalue=9e-88,
Organism=Escherichia coli, GI1786753, Length=281, Percent_Identity=55.5160142348754, Blast_Score=316, Evalue=9e-88,
Organism=Escherichia coli, GI1786570, Length=281, Percent_Identity=55.5160142348754, Blast_Score=316, Evalue=9e-88,
Organism=Escherichia coli, GI1786490, Length=281, Percent_Identity=55.5160142348754, Blast_Score=316, Evalue=9e-88,
Organism=Escherichia coli, GI1789981, Length=263, Percent_Identity=34.9809885931559, Blast_Score=154, Evalue=7e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001584
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00665 rve [H]

EC number: NA

Molecular weight: Translated: 32815; Mature: 32815

Theoretical pI: Translated: 9.29; Mature: 9.29

Prosite motif: PS50994 INTEGRASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLEHQLCFSVVRIAKVFCVSRSGFYYWIKHRHKALQREVTRQELDTRVKEAFDNSKGRDG
CCCHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCH
SRRIQKELAENGDSRNVKTIAASMKRQDLTPKAARKFKCTTDSKHKMPVAPNLLAQDFNA
HHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHCEECCCCCCCCCCCCCHHHHHCCCC
EAPNQKWAGDITYVATSEGWLYLAVIIDLYSRQVVGWSMDTRMTATLVCDALSMALFRRG
CCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCC
FPEQVIVHSDRGSQYCSKDYRDLITAYNLKQSMSRKGNCWDNACVESFFHSMKVEALQYE
CCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCC
PIMMRDEMRQAIFEYIEVDYNRTRRHSALGYLSPVNFEQQNVA
CHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MLEHQLCFSVVRIAKVFCVSRSGFYYWIKHRHKALQREVTRQELDTRVKEAFDNSKGRDG
CCCHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCH
SRRIQKELAENGDSRNVKTIAASMKRQDLTPKAARKFKCTTDSKHKMPVAPNLLAQDFNA
HHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHCEECCCCCCCCCCCCCHHHHHCCCC
EAPNQKWAGDITYVATSEGWLYLAVIIDLYSRQVVGWSMDTRMTATLVCDALSMALFRRG
CCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCC
FPEQVIVHSDRGSQYCSKDYRDLITAYNLKQSMSRKGNCWDNACVESFFHSMKVEALQYE
CCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCC
PIMMRDEMRQAIFEYIEVDYNRTRRHSALGYLSPVNFEQQNVA
CHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2987844; 9278503 [H]