Click here to switch to the map view.

The map label for this gene is folE [H]

Identifier: 156977448

GI number: 156977448

Start: 1416067

End: 1416720

Strand: Reverse

Name: folE [H]

Synonym: VIBHAR_06235

Alternate gene names: 156977448

Gene position: 1416720-1416067 (Counterclockwise)

Preceding gene: 156977451

Following gene: 156977445

Centisome position: 64.28

GC content: 46.94

Gene sequence:

>654_bases
ATGTCAGGTCTTAGCGAGTCAGCGAAGTTAGTAAAAGAAGCGCTAGAGCAACGCGGTTTGGAAACGCCAATGCGTCCAAA
CAACGTAAGTCGTGAAGAGAAGAAAGAGAAGATCGAACACCACATGCGTGAGATCTTAGGTCTACTTCAACTTGACCTTA
CTGACGATAGTTTAGAGGAAACACCTCACCGCATTGCTAAGATGTACGTTGATGAAATCTTCTCTGGTTTGGATTACTCC
AACTTCCCGAAAATCACGGTGATTGAAAACAAGATGAACGTGAGCGAAATGGTGCGCGTGAAGGACATCACAGTGACCAG
TACTTGTGAACACCACCTTGTGACCATCGATGGCAAAGCTGCTGTGGCTTACATTCCACGTGGCAAGATCATTGGTCTAT
CAAAGATCAACCGTATCGTTCGCTTCTTTGCCCAGCGTCCACAAGTTCAAGAGCGTATGACTCAGCAGATTCTTGTTGCA
CTTCAAACGTTGCTTGAGTCTGATGATGTGGCAGTGACGATTGACGCAACACACTACTGCGTGAAATCTCGTGGTGTGAT
GGATGCAACTAGCGAAACGACAACAACAGCATTGGGTGGCATCTTCAAAAGCAACCCAGCAACGCGTGCCGAGTTTCTGC
ACGGTTTACGCTAA

Upstream 100 bases:

>100_bases
CTTGGTGTGAAGACACGAACTGAGCAGCTCAAACCGAAAAGTATGAATGCAATTTTGATTGGGTTCGTATCAAAGCGGAA
GTAACAAAAGAGGAAGTGGA

Downstream 100 bases:

>100_bases
GTAACAAAGCAATATTCTAAGAGGATGGCAAACGCCATCCTTTTTTACATTTGCTTGGAGGGTGTGCTGATGAGTTCTGG
CCAGTACATTTTTACATAGC

Product: GTP cyclohydrolase I

Products: NA

Alternate protein names: GTP cyclohydrolase I; GTP-CH-I [H]

Number of amino acids: Translated: 217; Mature: 216

Protein sequence:

>217_residues
MSGLSESAKLVKEALEQRGLETPMRPNNVSREEKKEKIEHHMREILGLLQLDLTDDSLEETPHRIAKMYVDEIFSGLDYS
NFPKITVIENKMNVSEMVRVKDITVTSTCEHHLVTIDGKAAVAYIPRGKIIGLSKINRIVRFFAQRPQVQERMTQQILVA
LQTLLESDDVAVTIDATHYCVKSRGVMDATSETTTTALGGIFKSNPATRAEFLHGLR

Sequences:

>Translated_217_residues
MSGLSESAKLVKEALEQRGLETPMRPNNVSREEKKEKIEHHMREILGLLQLDLTDDSLEETPHRIAKMYVDEIFSGLDYS
NFPKITVIENKMNVSEMVRVKDITVTSTCEHHLVTIDGKAAVAYIPRGKIIGLSKINRIVRFFAQRPQVQERMTQQILVA
LQTLLESDDVAVTIDATHYCVKSRGVMDATSETTTTALGGIFKSNPATRAEFLHGLR
>Mature_216_residues
SGLSESAKLVKEALEQRGLETPMRPNNVSREEKKEKIEHHMREILGLLQLDLTDDSLEETPHRIAKMYVDEIFSGLDYSN
FPKITVIENKMNVSEMVRVKDITVTSTCEHHLVTIDGKAAVAYIPRGKIIGLSKINRIVRFFAQRPQVQERMTQQILVAL
QTLLESDDVAVTIDATHYCVKSRGVMDATSETTTTALGGIFKSNPATRAEFLHGLR

Specific function: Tetrahydrofolate biosynthesis; first step. [C]

COG id: COG0302

COG function: function code H; GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family [H]

Homologues:

Organism=Homo sapiens, GI66932968, Length=122, Percent_Identity=47.5409836065574, Blast_Score=120, Evalue=6e-28,
Organism=Homo sapiens, GI4503949, Length=122, Percent_Identity=47.5409836065574, Blast_Score=120, Evalue=6e-28,
Organism=Homo sapiens, GI66932970, Length=91, Percent_Identity=46.1538461538462, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI66932972, Length=82, Percent_Identity=50, Blast_Score=86, Evalue=3e-17,
Organism=Escherichia coli, GI1788476, Length=219, Percent_Identity=71.2328767123288, Blast_Score=307, Evalue=4e-85,
Organism=Caenorhabditis elegans, GI17560486, Length=206, Percent_Identity=36.4077669902913, Blast_Score=128, Evalue=2e-30,
Organism=Saccharomyces cerevisiae, GI6321706, Length=180, Percent_Identity=40, Blast_Score=122, Evalue=3e-29,
Organism=Drosophila melanogaster, GI24656782, Length=192, Percent_Identity=39.0625, Blast_Score=116, Evalue=1e-26,
Organism=Drosophila melanogaster, GI24656791, Length=192, Percent_Identity=39.0625, Blast_Score=116, Evalue=1e-26,
Organism=Drosophila melanogaster, GI24656796, Length=192, Percent_Identity=39.0625, Blast_Score=116, Evalue=1e-26,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001474
- InterPro:   IPR020602
- InterPro:   IPR018234 [H]

Pfam domain/function: PF01227 GTP_cyclohydroI [H]

EC number: =3.5.4.16 [H]

Molecular weight: Translated: 24394; Mature: 24263

Theoretical pI: Translated: 6.89; Mature: 6.89

Prosite motif: PS00859 GTP_CYCLOHYDROL_1_1 ; PS00860 GTP_CYCLOHYDROL_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGLSESAKLVKEALEQRGLETPMRPNNVSREEKKEKIEHHMREILGLLQLDLTDDSLEE
CCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
TPHRIAKMYVDEIFSGLDYSNFPKITVIENKMNVSEMVRVKDITVTSTCEHHLVTIDGKA
HHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHCEEEECCCCCEEEEECCCE
AVAYIPRGKIIGLSKINRIVRFFAQRPQVQERMTQQILVALQTLLESDDVAVTIDATHYC
EEEEECCCCEECHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCEEEEECHHHHH
VKSRGVMDATSETTTTALGGIFKSNPATRAEFLHGLR
HHCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHCCC
>Mature Secondary Structure 
SGLSESAKLVKEALEQRGLETPMRPNNVSREEKKEKIEHHMREILGLLQLDLTDDSLEE
CCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
TPHRIAKMYVDEIFSGLDYSNFPKITVIENKMNVSEMVRVKDITVTSTCEHHLVTIDGKA
HHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHCEEEECCCCCEEEEECCCE
AVAYIPRGKIIGLSKINRIVRFFAQRPQVQERMTQQILVALQTLLESDDVAVTIDATHYC
EEEEECCCCEECHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCEEEEECHHHHH
VKSRGVMDATSETTTTALGGIFKSNPATRAEFLHGLR
HHCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]