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The map label for this gene is mbaA [H]

Identifier: 156977361

GI number: 156977361

Start: 1325795

End: 1328131

Strand: Direct

Name: mbaA [H]

Synonym: VIBHAR_06147

Alternate gene names: 156977361

Gene position: 1325795-1328131 (Clockwise)

Preceding gene: 156977350

Following gene: 156977363

Centisome position: 60.15

GC content: 44.07

Gene sequence:

>2337_bases
ATGAAATTAAGCAACAGAGTCGTTTTGCTTGTCGCCCCTGTCATTCTAATTAGCGCGCTGGTGTCGAGTTACATCATCTA
TAGCATTCAAAAAGTCACGCTAATCAAAAGGGAAGACAGCTATATCCAGCTACAAATGGAAAAGCTAGCTGGTCAGTTTC
GCCAAGCCAATATTTTTCTTAACAGCTATGCCTACACCTTGAGTAAAAGCGAGGTGCTGCAAGATTATTTTGTGAATCAT
GATAACCACTATCGTGAGAGGCAACTATTCAAACGTTTAGATGAAACCATCGAGGTACTCAGTCACGGGTATCGAGGGGA
TGCAAATATGGCGATCCTCGACGGTAGGCAAGACCTCCTCTACTACACCGAAAATCAATATTACGAATCAAACGGTCGAG
TCGACCCCAAGATCCTTGAGTATGTCGACAAAAGCTTTAAAACCAATGGAGACGTAAGCCATACCGGTTTTATTTACAAC
AGCAGCGGACAGAGCATTCTATTGCAATACGAGCTGTTTGATAAACGTACCGGAGAGCACCCTTTAAGCTTCGACCCACA
AAATGTTTTCTTTGTTATCGTGTCTGTCTCACTCGAAGCGTTTAATGACATCAAGCATGAGATTGAGTTTGATACGCACA
GCGTGATTACGTTTTCAGATAAGCCGATTATCTTAGATATTCCCCTTGCTCAAACCATCCAGTTTCTGCCTCACTTCTAT
GCCATTTTATCGCCAGCAGAATATCTGATGTGGAATAAGGTCGATAAGGTCTGGTTAGAGCTAGCATTGTCTTTTGGTAT
CGCATCTTTTTGTACCATTACTTTAATTGTCTTAGTCCTTTACCGCTATGTTCTGAGTCCAGTCTCTAGGCTAGACAAAC
AGTTGAGAGAAATCGAAGCCAACAAACGCGCAAATATTGAAAAGCTCGATACTGATGATGAGATTGGACGCCTTTCTTCC
CGCTTCTTTGATATGTACGAAGAGCTGAACAACGTCTACCACAAAACTAAGCGTTTAGCGGAAACTGATCACTTAACTCA
ACTGGCTAATCGCCACCGCTTCCATGAGCTTGCAAGCCAAGAGCTGGCAGAACCCTCAGAGCAGATTTGGGTGATTTATA
TCGACCTAGATAACTTTAAATACGTCAACGACAAATACGGCCACGAACTGGGTGACAAGTTATTGCAGGTCTTCTCCAAA
CACATCCGAAATGCGTGCCAGAAGTTCACTCAGCAGTACAATAGCCCATGTTTCGGGGCACGTTTATCCGGTGATGAATT
TGCAATCCTACTCAGCTCAAATCAAGAAAACAGCGAATTACCCGATCTGTTTGCCGCGGAGTTGCTCAAGCCAATTAATA
ACTTAACTCAGTCAGCAAAAAACTCGTTTCCGGTGACAGCCAGCGTGGGTATCGCTCAATATCCAAGAGATGGTGGTGAT
ATCGCTAAGCTCTTGTCTAGTGCAGACGCGGCAATGTACCAAGCTAAGCGCGCGGGTAAAAACCAATACGCATACTACTC
TGCAGCGCTCAATATCGAGGCTCAACGTCGTAGCCAAATTGAACGCGCATTGCGCAAGGCCAACGTTGAAGAAGAGTTTC
GTCTTGTTTTCCAACCATACATGAACAGTAATGCCAACGAGATAGAAGGCTTGGAAGTCCTATTACGTTGGGAAGCAGAA
GGGATCGGCTCAGTACCACCAAACGAGTTCATCCCTATTGCTGAACAAACTGGTTTGTTCGAAAAGATTGACCGCTGGGT
ATTCGCGAATGCCATGGCAGAATACTATCAGCTGAAAAGCATCTTTGGGAAAGACATTATTTTGTCTATCAACCTATCGT
CTGCGGAACTGAACTCGCTGTCGATGGCAGAATTCATCCATGAAAAAGCCACACAGAATGGTGTGCCTACGCGCTGTATT
GAGCTAGAAATTACCGAAACTTTCGCGTCTGACAAGCAAGGTACTGCCCTACTCGATGAGTTATCGAAGCTGGGTTACAA
ACTGGCAATAGACGACTTTGGATCTGGTTATACTTCTTTGACACAACTAGTGCAGTACCCAGTACAGAAAATCAAATTTG
ACCGAGAGTTCTTGTTGACCTTAATGAACACCGATAATGGTCATGTCATTAAACCAATTATAGAGCTGTGCCACGCACAG
AATAAGAAAGTGACGGCAGAAGGCATTGAGCATAACGTCATGCACGAATGGCTTTCATCTTACCGCTGTGACTTTATGCA
GGGTTATTTATTTGGTAAGCCGATGAACAAAGAACAGCTCGGCCATTGGTGGAAGTCCGCTAATGATCAGCCATTTAAAA
GCACACATATCGCTTAG

Upstream 100 bases:

>100_bases
AAAGTATCATCATCCGTTCGATACCTTAGGTGAGAACCAACGGGTAAAAACCATTAGGTTAGAAGCGTTTGTAAGGACAC
AAACGGAAAGTAAGAAATAC

Downstream 100 bases:

>100_bases
TAGGTAAAACATACCGCAAACGAAAAGCAGCACGCTAAGTGCTGCTTTTTCCTCTTGTTTCGGCTAGGAAAAGCTTACTG
AAAGAAAAGTTTATTCTGGT

Product: diguanylate cyclase/phosphodiesterase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 778; Mature: 778

Protein sequence:

>778_residues
MKLSNRVVLLVAPVILISALVSSYIIYSIQKVTLIKREDSYIQLQMEKLAGQFRQANIFLNSYAYTLSKSEVLQDYFVNH
DNHYRERQLFKRLDETIEVLSHGYRGDANMAILDGRQDLLYYTENQYYESNGRVDPKILEYVDKSFKTNGDVSHTGFIYN
SSGQSILLQYELFDKRTGEHPLSFDPQNVFFVIVSVSLEAFNDIKHEIEFDTHSVITFSDKPIILDIPLAQTIQFLPHFY
AILSPAEYLMWNKVDKVWLELALSFGIASFCTITLIVLVLYRYVLSPVSRLDKQLREIEANKRANIEKLDTDDEIGRLSS
RFFDMYEELNNVYHKTKRLAETDHLTQLANRHRFHELASQELAEPSEQIWVIYIDLDNFKYVNDKYGHELGDKLLQVFSK
HIRNACQKFTQQYNSPCFGARLSGDEFAILLSSNQENSELPDLFAAELLKPINNLTQSAKNSFPVTASVGIAQYPRDGGD
IAKLLSSADAAMYQAKRAGKNQYAYYSAALNIEAQRRSQIERALRKANVEEEFRLVFQPYMNSNANEIEGLEVLLRWEAE
GIGSVPPNEFIPIAEQTGLFEKIDRWVFANAMAEYYQLKSIFGKDIILSINLSSAELNSLSMAEFIHEKATQNGVPTRCI
ELEITETFASDKQGTALLDELSKLGYKLAIDDFGSGYTSLTQLVQYPVQKIKFDREFLLTLMNTDNGHVIKPIIELCHAQ
NKKVTAEGIEHNVMHEWLSSYRCDFMQGYLFGKPMNKEQLGHWWKSANDQPFKSTHIA

Sequences:

>Translated_778_residues
MKLSNRVVLLVAPVILISALVSSYIIYSIQKVTLIKREDSYIQLQMEKLAGQFRQANIFLNSYAYTLSKSEVLQDYFVNH
DNHYRERQLFKRLDETIEVLSHGYRGDANMAILDGRQDLLYYTENQYYESNGRVDPKILEYVDKSFKTNGDVSHTGFIYN
SSGQSILLQYELFDKRTGEHPLSFDPQNVFFVIVSVSLEAFNDIKHEIEFDTHSVITFSDKPIILDIPLAQTIQFLPHFY
AILSPAEYLMWNKVDKVWLELALSFGIASFCTITLIVLVLYRYVLSPVSRLDKQLREIEANKRANIEKLDTDDEIGRLSS
RFFDMYEELNNVYHKTKRLAETDHLTQLANRHRFHELASQELAEPSEQIWVIYIDLDNFKYVNDKYGHELGDKLLQVFSK
HIRNACQKFTQQYNSPCFGARLSGDEFAILLSSNQENSELPDLFAAELLKPINNLTQSAKNSFPVTASVGIAQYPRDGGD
IAKLLSSADAAMYQAKRAGKNQYAYYSAALNIEAQRRSQIERALRKANVEEEFRLVFQPYMNSNANEIEGLEVLLRWEAE
GIGSVPPNEFIPIAEQTGLFEKIDRWVFANAMAEYYQLKSIFGKDIILSINLSSAELNSLSMAEFIHEKATQNGVPTRCI
ELEITETFASDKQGTALLDELSKLGYKLAIDDFGSGYTSLTQLVQYPVQKIKFDREFLLTLMNTDNGHVIKPIIELCHAQ
NKKVTAEGIEHNVMHEWLSSYRCDFMQGYLFGKPMNKEQLGHWWKSANDQPFKSTHIA
>Mature_778_residues
MKLSNRVVLLVAPVILISALVSSYIIYSIQKVTLIKREDSYIQLQMEKLAGQFRQANIFLNSYAYTLSKSEVLQDYFVNH
DNHYRERQLFKRLDETIEVLSHGYRGDANMAILDGRQDLLYYTENQYYESNGRVDPKILEYVDKSFKTNGDVSHTGFIYN
SSGQSILLQYELFDKRTGEHPLSFDPQNVFFVIVSVSLEAFNDIKHEIEFDTHSVITFSDKPIILDIPLAQTIQFLPHFY
AILSPAEYLMWNKVDKVWLELALSFGIASFCTITLIVLVLYRYVLSPVSRLDKQLREIEANKRANIEKLDTDDEIGRLSS
RFFDMYEELNNVYHKTKRLAETDHLTQLANRHRFHELASQELAEPSEQIWVIYIDLDNFKYVNDKYGHELGDKLLQVFSK
HIRNACQKFTQQYNSPCFGARLSGDEFAILLSSNQENSELPDLFAAELLKPINNLTQSAKNSFPVTASVGIAQYPRDGGD
IAKLLSSADAAMYQAKRAGKNQYAYYSAALNIEAQRRSQIERALRKANVEEEFRLVFQPYMNSNANEIEGLEVLLRWEAE
GIGSVPPNEFIPIAEQTGLFEKIDRWVFANAMAEYYQLKSIFGKDIILSINLSSAELNSLSMAEFIHEKATQNGVPTRCI
ELEITETFASDKQGTALLDELSKLGYKLAIDDFGSGYTSLTQLVQYPVQKIKFDREFLLTLMNTDNGHVIKPIIELCHAQ
NKKVTAEGIEHNVMHEWLSSYRCDFMQGYLFGKPMNKEQLGHWWKSANDQPFKSTHIA

Specific function: Plays an essential role in the maintenance and the formation of the three-dimensional structure of the biofilms at the later stages of their development. Absence of mbaA promotes the accumulation of larger amount of biomass on the surfaces at later stage

COG id: COG2200

COG function: function code T; FOG: EAL domain

Gene ontology:

Cell location: Cell inner membrane; Single-pass type I membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HAMP domain [H]

Homologues:

Organism=Escherichia coli, GI1787541, Length=446, Percent_Identity=30.0448430493274, Blast_Score=205, Evalue=9e-54,
Organism=Escherichia coli, GI87081921, Length=438, Percent_Identity=28.9954337899543, Blast_Score=177, Evalue=3e-45,
Organism=Escherichia coli, GI1790496, Length=248, Percent_Identity=32.258064516129, Blast_Score=128, Evalue=1e-30,
Organism=Escherichia coli, GI226510982, Length=536, Percent_Identity=24.0671641791045, Blast_Score=122, Evalue=1e-28,
Organism=Escherichia coli, GI1788502, Length=251, Percent_Identity=29.4820717131474, Blast_Score=117, Evalue=3e-27,
Organism=Escherichia coli, GI1787055, Length=373, Percent_Identity=25.201072386059, Blast_Score=114, Evalue=2e-26,
Organism=Escherichia coli, GI87081743, Length=232, Percent_Identity=33.1896551724138, Blast_Score=108, Evalue=1e-24,
Organism=Escherichia coli, GI1786507, Length=236, Percent_Identity=27.1186440677966, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI87081980, Length=263, Percent_Identity=28.8973384030418, Blast_Score=101, Evalue=2e-22,
Organism=Escherichia coli, GI87081845, Length=242, Percent_Identity=27.2727272727273, Blast_Score=92, Evalue=9e-20,
Organism=Escherichia coli, GI1788849, Length=245, Percent_Identity=26.9387755102041, Blast_Score=89, Evalue=9e-19,
Organism=Escherichia coli, GI87082096, Length=443, Percent_Identity=24.8306997742664, Blast_Score=88, Evalue=3e-18,
Organism=Escherichia coli, GI1787802, Length=180, Percent_Identity=31.1111111111111, Blast_Score=82, Evalue=9e-17,
Organism=Escherichia coli, GI1788381, Length=321, Percent_Identity=27.1028037383178, Blast_Score=82, Evalue=1e-16,
Organism=Escherichia coli, GI145693134, Length=172, Percent_Identity=31.3953488372093, Blast_Score=77, Evalue=4e-15,
Organism=Escherichia coli, GI1787262, Length=171, Percent_Identity=29.8245614035088, Blast_Score=69, Evalue=1e-12,
Organism=Escherichia coli, GI1786584, Length=167, Percent_Identity=25.748502994012, Blast_Score=67, Evalue=5e-12,
Organism=Escherichia coli, GI1787816, Length=167, Percent_Identity=26.9461077844311, Blast_Score=66, Evalue=8e-12,
Organism=Escherichia coli, GI1788956, Length=256, Percent_Identity=27.34375, Blast_Score=66, Evalue=1e-11,
Organism=Escherichia coli, GI87081977, Length=167, Percent_Identity=32.3353293413174, Blast_Score=65, Evalue=2e-11,
Organism=Escherichia coli, GI87082007, Length=166, Percent_Identity=28.9156626506024, Blast_Score=65, Evalue=2e-11,
Organism=Escherichia coli, GI1788085, Length=192, Percent_Identity=27.6041666666667, Blast_Score=64, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR001633
- InterPro:   IPR003660 [H]

Pfam domain/function: PF00563 EAL; PF00990 GGDEF; PF00672 HAMP [H]

EC number: NA

Molecular weight: Translated: 89322; Mature: 89322

Theoretical pI: Translated: 5.53; Mature: 5.53

Prosite motif: PS50885 HAMP ; PS50883 EAL ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLSNRVVLLVAPVILISALVSSYIIYSIQKVTLIKREDSYIQLQMEKLAGQFRQANIFL
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCEEEHHHHHHHHHHHEEEE
NSYAYTLSKSEVLQDYFVNHDNHYRERQLFKRLDETIEVLSHGYRGDANMAILDGRQDLL
EHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCEE
YYTENQYYESNGRVDPKILEYVDKSFKTNGDVSHTGFIYNSSGQSILLQYELFDKRTGEH
EEECCCEECCCCCCCHHHHHHHHHHHCCCCCCCCCEEEECCCCCEEEEEEEEHHCCCCCC
PLSFDPQNVFFVIVSVSLEAFNDIKHEIEFDTHSVITFSDKPIILDIPLAQTIQFLPHFY
CCCCCCCCEEEEEEEHHHHHHHHHHHHCCCCCCEEEEECCCCEEEECCHHHHHHHHHHHH
AILSPAEYLMWNKVDKVWLELALSFGIASFCTITLIVLVLYRYVLSPVSRLDKQLREIEA
HHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NKRANIEKLDTDDEIGRLSSRFFDMYEELNNVYHKTKRLAETDHLTQLANRHRFHELASQ
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ELAEPSEQIWVIYIDLDNFKYVNDKYGHELGDKLLQVFSKHIRNACQKFTQQYNSPCFGA
HHCCCCCCEEEEEEECCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
RLSGDEFAILLSSNQENSELPDLFAAELLKPINNLTQSAKNSFPVTASVGIAQYPRDGGD
EECCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEECCHHHCCCCCHH
IAKLLSSADAAMYQAKRAGKNQYAYYSAALNIEAQRRSQIERALRKANVEEEFRLVFQPY
HHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCHHHHHHHHHHHHHCCCHHHHHHHHHHH
MNSNANEIEGLEVLLRWEAEGIGSVPPNEFIPIAEQTGLFEKIDRWVFANAMAEYYQLKS
HCCCCCHHHHHHHEEEECCCCCCCCCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHH
IFGKDIILSINLSSAELNSLSMAEFIHEKATQNGVPTRCIELEITETFASDKQGTALLDE
HCCCCEEEEEECCHHHCCHHHHHHHHHHHHHCCCCCCEEEEEEEHHHHCCCCCCHHHHHH
LSKLGYKLAIDDFGSGYTSLTQLVQYPVQKIKFDREFLLTLMNTDNGHVIKPIIELCHAQ
HHHCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCHHHHHHHHCCC
NKKVTAEGIEHNVMHEWLSSYRCDFMQGYLFGKPMNKEQLGHWWKSANDQPFKSTHIA
CCCEEHHHHHHHHHHHHHHHHCHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MKLSNRVVLLVAPVILISALVSSYIIYSIQKVTLIKREDSYIQLQMEKLAGQFRQANIFL
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCEEEHHHHHHHHHHHEEEE
NSYAYTLSKSEVLQDYFVNHDNHYRERQLFKRLDETIEVLSHGYRGDANMAILDGRQDLL
EHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCEE
YYTENQYYESNGRVDPKILEYVDKSFKTNGDVSHTGFIYNSSGQSILLQYELFDKRTGEH
EEECCCEECCCCCCCHHHHHHHHHHHCCCCCCCCCEEEECCCCCEEEEEEEEHHCCCCCC
PLSFDPQNVFFVIVSVSLEAFNDIKHEIEFDTHSVITFSDKPIILDIPLAQTIQFLPHFY
CCCCCCCCEEEEEEEHHHHHHHHHHHHCCCCCCEEEEECCCCEEEECCHHHHHHHHHHHH
AILSPAEYLMWNKVDKVWLELALSFGIASFCTITLIVLVLYRYVLSPVSRLDKQLREIEA
HHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NKRANIEKLDTDDEIGRLSSRFFDMYEELNNVYHKTKRLAETDHLTQLANRHRFHELASQ
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ELAEPSEQIWVIYIDLDNFKYVNDKYGHELGDKLLQVFSKHIRNACQKFTQQYNSPCFGA
HHCCCCCCEEEEEEECCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
RLSGDEFAILLSSNQENSELPDLFAAELLKPINNLTQSAKNSFPVTASVGIAQYPRDGGD
EECCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEECCHHHCCCCCHH
IAKLLSSADAAMYQAKRAGKNQYAYYSAALNIEAQRRSQIERALRKANVEEEFRLVFQPY
HHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCHHHHHHHHHHHHHCCCHHHHHHHHHHH
MNSNANEIEGLEVLLRWEAEGIGSVPPNEFIPIAEQTGLFEKIDRWVFANAMAEYYQLKS
HCCCCCHHHHHHHEEEECCCCCCCCCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHH
IFGKDIILSINLSSAELNSLSMAEFIHEKATQNGVPTRCIELEITETFASDKQGTALLDE
HCCCCEEEEEECCHHHCCHHHHHHHHHHHHHCCCCCCEEEEEEEHHHHCCCCCCHHHHHH
LSKLGYKLAIDDFGSGYTSLTQLVQYPVQKIKFDREFLLTLMNTDNGHVIKPIIELCHAQ
HHHCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCHHHHHHHHCCC
NKKVTAEGIEHNVMHEWLSSYRCDFMQGYLFGKPMNKEQLGHWWKSANDQPFKSTHIA
CCCEEHHHHHHHHHHHHHHHHCHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]