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The map label for this gene is rbsR [H]

Identifier: 156977355

GI number: 156977355

Start: 1319210

End: 1320220

Strand: Reverse

Name: rbsR [H]

Synonym: VIBHAR_06141

Alternate gene names: 156977355

Gene position: 1320220-1319210 (Counterclockwise)

Preceding gene: 156977356

Following gene: 156977354

Centisome position: 59.9

GC content: 45.7

Gene sequence:

>1011_bases
ATGGCAACGATGAAAGACATCGCAAAACTGGCAGGGGTTTCCACTTCAACTGTGAGCCATGTTATTAACAAAACTCGCTT
TGTCAGCGAAGAGATCTCGGAACGAGTCAATAACGCTGCCAAAGAGCTTAATTACTACGCGCCGTCTGCTTTGGCGCGTA
GCCTCAAAGTAAACCGCACCAAAACCATTGGTATGCTGGTTACTACCTCAACCAACCCATTCTTTGGTGAAGTCGTAAAA
GGTGTTGAGCGCAGTTGCTACCAAAAAGGTTACAGCCTTATCCTTTGTAATACCGAAGGAGATAACGAGCGCATGCGTGA
ATCTATCAATACGTTGTTGCAAAAGCGCGTTGATGGTCTGATTTTAATGTGTTCATCGTTAGAAGGGGAGCGTATTGATG
TATTCGAACGTTACCCTGATATCCCGGTTGTAGTGATGGACTGGGGCCCTATGTTGTTCACCAGCGATAAGATTCAAGAC
AACTCACTTCGTGGTGGTTATCTGGCAGCGAAATACCTTATCGACTGCGGCCACAAAGAGGTAGGCTGTATCACTGGTCC
ATTAATTAAGCACCAAGCGCAAATGCGTTATAAAGGCTACAAACGCGCGATGCTCGAAGCAGAACTTGAGTTCAATGCAA
ACTGGATTGTTGAATCTGATTTCGAATGTGAGGGTGGTTATCAAGCCTTCAAGAAAATGTTGGAACGCGGCCCACTACCT
AGCTCGATCTTTGTCTCCAATGACATGATGGCGATGGGCGTTATCAATGCAGCTAATGAACTCGGTATTCAAATTCCAGA
ACAGCTTTCTATCATAGGTTACGATGATATTCATATTGCGAAGTTTATGTCGCCTTCGCTTACCACGATTCACCAGCCAA
AATACCGTCTAGGACAAGCCGCAGTAGAGACACTGTTACGCAAACTGGACGAGAAGTCTGTAGAAGCTCAGGTTGTGCAG
CTTGAACCAACATTGGTTGAGCGCAACAGTGTGAAGTGCCTCTCCGAATAA

Upstream 100 bases:

>100_bases
CTGCGATCTCAGTAACTCGCTTTGGCGCGCAAACCTCCATTCCAACCCGTAAAGAGGTTGAGGAGTTCCTTGAACAACAA
TAATCAAAAGGAGAAATAGC

Downstream 100 bases:

>100_bases
AGAAGTTATTCGCTCTCAATTAGTTATTTATCTAAACTTGTAATAATCAGCTTTCTGAATGGATTTAGAAAGTAAATGCC
TTCTTTTTACATAATAAGCC

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 336; Mature: 335

Protein sequence:

>336_residues
MATMKDIAKLAGVSTSTVSHVINKTRFVSEEISERVNNAAKELNYYAPSALARSLKVNRTKTIGMLVTTSTNPFFGEVVK
GVERSCYQKGYSLILCNTEGDNERMRESINTLLQKRVDGLILMCSSLEGERIDVFERYPDIPVVVMDWGPMLFTSDKIQD
NSLRGGYLAAKYLIDCGHKEVGCITGPLIKHQAQMRYKGYKRAMLEAELEFNANWIVESDFECEGGYQAFKKMLERGPLP
SSIFVSNDMMAMGVINAANELGIQIPEQLSIIGYDDIHIAKFMSPSLTTIHQPKYRLGQAAVETLLRKLDEKSVEAQVVQ
LEPTLVERNSVKCLSE

Sequences:

>Translated_336_residues
MATMKDIAKLAGVSTSTVSHVINKTRFVSEEISERVNNAAKELNYYAPSALARSLKVNRTKTIGMLVTTSTNPFFGEVVK
GVERSCYQKGYSLILCNTEGDNERMRESINTLLQKRVDGLILMCSSLEGERIDVFERYPDIPVVVMDWGPMLFTSDKIQD
NSLRGGYLAAKYLIDCGHKEVGCITGPLIKHQAQMRYKGYKRAMLEAELEFNANWIVESDFECEGGYQAFKKMLERGPLP
SSIFVSNDMMAMGVINAANELGIQIPEQLSIIGYDDIHIAKFMSPSLTTIHQPKYRLGQAAVETLLRKLDEKSVEAQVVQ
LEPTLVERNSVKCLSE
>Mature_335_residues
ATMKDIAKLAGVSTSTVSHVINKTRFVSEEISERVNNAAKELNYYAPSALARSLKVNRTKTIGMLVTTSTNPFFGEVVKG
VERSCYQKGYSLILCNTEGDNERMRESINTLLQKRVDGLILMCSSLEGERIDVFERYPDIPVVVMDWGPMLFTSDKIQDN
SLRGGYLAAKYLIDCGHKEVGCITGPLIKHQAQMRYKGYKRAMLEAELEFNANWIVESDFECEGGYQAFKKMLERGPLPS
SIFVSNDMMAMGVINAANELGIQIPEQLSIIGYDDIHIAKFMSPSLTTIHQPKYRLGQAAVETLLRKLDEKSVEAQVVQL
EPTLVERNSVKCLSE

Specific function: Transcriptional repressor for the ribose rbsDACBK operon. RbsR binds to a region of perfect dyad symmetry spanning the rbs operon transcriptional start site. The affinity for the rbs operator is reduced by addition of ribose, consistent with ribose being

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790194, Length=330, Percent_Identity=54.8484848484848, Blast_Score=390, Evalue=1e-110,
Organism=Escherichia coli, GI1787948, Length=333, Percent_Identity=48.3483483483483, Blast_Score=333, Evalue=1e-92,
Organism=Escherichia coli, GI1790369, Length=335, Percent_Identity=32.8358208955224, Blast_Score=198, Evalue=4e-52,
Organism=Escherichia coli, GI1789202, Length=336, Percent_Identity=31.8452380952381, Blast_Score=170, Evalue=1e-43,
Organism=Escherichia coli, GI1787580, Length=316, Percent_Identity=31.6455696202532, Blast_Score=166, Evalue=1e-42,
Organism=Escherichia coli, GI1789068, Length=312, Percent_Identity=31.7307692307692, Blast_Score=162, Evalue=3e-41,
Organism=Escherichia coli, GI1788474, Length=336, Percent_Identity=31.25, Blast_Score=162, Evalue=3e-41,
Organism=Escherichia coli, GI1786540, Length=335, Percent_Identity=29.8507462686567, Blast_Score=147, Evalue=7e-37,
Organism=Escherichia coli, GI1790715, Length=336, Percent_Identity=27.0833333333333, Blast_Score=115, Evalue=4e-27,
Organism=Escherichia coli, GI48994940, Length=330, Percent_Identity=26.3636363636364, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI1787906, Length=324, Percent_Identity=23.4567901234568, Blast_Score=109, Evalue=3e-25,
Organism=Escherichia coli, GI1786268, Length=318, Percent_Identity=27.0440251572327, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1789456, Length=326, Percent_Identity=28.2208588957055, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1790689, Length=325, Percent_Identity=22.1538461538462, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1790192, Length=268, Percent_Identity=23.5074626865672, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 37599; Mature: 37468

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATMKDIAKLAGVSTSTVSHVINKTRFVSEEISERVNNAAKELNYYAPSALARSLKVNRT
CCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCE
KTIGMLVTTSTNPFFGEVVKGVERSCYQKGYSLILCNTEGDNERMRESINTLLQKRVDGL
EEEEEEEEECCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCE
ILMCSSLEGERIDVFERYPDIPVVVMDWGPMLFTSDKIQDNSLRGGYLAAKYLIDCGHKE
EEEECCCCCCEEHHHHHCCCCCEEEEECCCEEEECCCCCCCCCCCHHHHHHHHHHCCCCC
VGCITGPLIKHQAQMRYKGYKRAMLEAELEFNANWIVESDFECEGGYQAFKKMLERGPLP
CCCCCCHHHHHHHHHHHHHHHHHHHHEEEEECCEEEEECCCCCCCCHHHHHHHHHHCCCC
SSIFVSNDMMAMGVINAANELGIQIPEQLSIIGYDDIHIAKFMSPSLTTIHQPKYRLGQA
CEEEECCCCHHHHHHHHHHHCCCCCCCCEEEECCCCHHHHHHCCCCCCEECCCHHHHHHH
AVETLLRKLDEKSVEAQVVQLEPTLVERNSVKCLSE
HHHHHHHHHCCCCCCEEEEEECCHHCCCCCCCCCCC
>Mature Secondary Structure 
ATMKDIAKLAGVSTSTVSHVINKTRFVSEEISERVNNAAKELNYYAPSALARSLKVNRT
CCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCE
KTIGMLVTTSTNPFFGEVVKGVERSCYQKGYSLILCNTEGDNERMRESINTLLQKRVDGL
EEEEEEEEECCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCE
ILMCSSLEGERIDVFERYPDIPVVVMDWGPMLFTSDKIQDNSLRGGYLAAKYLIDCGHKE
EEEECCCCCCEEHHHHHCCCCCEEEEECCCEEEECCCCCCCCCCCHHHHHHHHHHCCCCC
VGCITGPLIKHQAQMRYKGYKRAMLEAELEFNANWIVESDFECEGGYQAFKKMLERGPLP
CCCCCCHHHHHHHHHHHHHHHHHHHHEEEEECCEEEEECCCCCCCCHHHHHHHHHHCCCC
SSIFVSNDMMAMGVINAANELGIQIPEQLSIIGYDDIHIAKFMSPSLTTIHQPKYRLGQA
CEEEECCCCHHHHHHHHHHHCCCCCCCCEEEECCCCHHHHHHCCCCCCEECCCHHHHHHH
AVETLLRKLDEKSVEAQVVQLEPTLVERNSVKCLSE
HHHHHHHHHCCCCCCEEEEEECCHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]