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The map label for this gene is helD [H]

Identifier: 156977336

GI number: 156977336

Start: 1286312

End: 1288381

Strand: Reverse

Name: helD [H]

Synonym: VIBHAR_06122

Alternate gene names: 156977336

Gene position: 1288381-1286312 (Counterclockwise)

Preceding gene: 156977342

Following gene: 156977333

Centisome position: 58.46

GC content: 46.14

Gene sequence:

>2070_bases
ATGCAGCTTACCGCCTCTAATAAAGCACAGTTTTTTATTCAAAATGAGTATTACCAAGTCGAAATAAAAGAGAACAGTGT
TTTATTAAGTTCAGTGGGTAGTGAAGAGCACATCCCATTTACTATTTGGAATGGCAAAGTGAGCGTTAAACGCGGTCTAC
TTTGGAGTTCTCTTCAGTTCTTTGCTCATGAGCAAGATGGCAAGCAACAAAGTTGGCTCGTTCAAGGTTTGTCTTGGCCA
CATTGTCGTAAGTTTGCGGTTGAAGCGGTACGTCAATATCAAGATTGGCACAATACCCAGTGCCGCAAACTCGCGGAATA
CCTTCCTAAGTGGGAAGATGAGTTGTATCAACTTAAACACCTTCCGGCTTATCTATCGCATTCAAAAGTTCAAACCTGGG
TCGACCAACTCAACAGTGAATTAGTTGAAATCAAAACCAGCTTGGTGGAAGCGAAAATGCGTATGCCCAAGCGAATGGCT
GAGATTGAACCGTGGCTTTCAGAGACTTCTCAAACGCTGAGCGAGCGAAATCACGAGTGGCTTGAGAATGAACGCCCGAA
TTGGGAAGTGCTATTTAGCCGGATCGAATCTTCACCATTGAATCTCTCGCAGCAGCATGCTGTGCTTCTCAATGATGATC
ACAACCTTGTTTTAGCGGGGGCAGGTTCCGGTAAGACGAGTGTACTGACGGCGCGTGTGGCGTATTTACTGCAAAGTCAC
CAAACACAAGCGGAAGAGCTTTTGATGCTAGCCTTTGGTCGTGACGCTGCGAAAGAGATGAAAGAGCGCTTGGTCGATAA
AGTGGGCTTGGCGGCAGAAGGCGTTCGCGTGAATACTTTTCACCAACTTGGTCTGCGCATTTTAAACCAAGTTGAACCTC
AGCCGGTAGAAATCAGCCCACTTGCTTTAGATGACAATCAACGTACAGCATGGTGCATCGATTGGCTCAAGAAGCACTGG
ATGACACCGACTAATTTTAAGCGCTGGCAAAAGCACTTAGATAAGTGGCCGATTGCGTACTTGAAAGGTGATGATGAGCT
AGGTAGTCACTCCGAAAACCCGAAATTGATCGCTTGGCTAGACAATCAACTGTCACAGCTTGCTGCGGTTGGCCTGACTA
AAAAGCAAGTGCAAGAAAAGCTGGTGAATCACCAAGACTATACGCGCTTGAACAGTGAGCTTGCACTGTGTTGGCCGTGC
TTTAGTGCGTGGCAAAAAATGCTAAAAGAGACCAATCAGGTGGATTTCCCAACGATGATCAGTCGAGCGACGGATTACGT
TAAAAAAGGGAAATACATTTCGCCTTGGCGCTTTATTATGGTGGATGAATACCAAGATATCTCTCCTGACCGTTTAGCGC
TTATAGAAGCGCTATGTGAGTCAACAGAGAAGCAAGCGGGAGCAACCTTATTTGCTGTCGGCGATGATTGGCAGGCAATT
TATCAGTTTGCAGGGGCCGATGTGGATCTCACAACGGGCTTCCAAGATCGATTCACACACTCAACCGTACATCATTTAGA
CACCACGTACCGCTTCAACAACCAAATCGGTGATGTGGCAAATACCTTTGTACAAGAGAACCCATCTCAGTTGCCAAAGA
CCCTGAACAGTCATAAGCAACGTAAGCAGAAGAGCGTGCATACTGCACCAAGCAATCAAGTTGAGAAGATTCTTGAACAG
CTTAACCAACAGGCGAAGAAAACCAAATCAGTATTGCTACTGGGTCGTAACCATTACCACAAGCCTGATTTATATGATGA
CTGGTTGAAGCGATTCCCTAAACTTGAGATTCGCTTTATGACCTGCCACGCGAGTAAGGGCAGGGAAGCAGACTTTGTAA
TCATCCTATCTGTGGATGAAGGCCAGTTCCCAGCGAAGAAGAAGCAAGCGCATATCGATGGGGCGTTGAGTGAATCGAAA
GACAAATATCCGTATGCCGAAGAGCGTCGTTTGTTCTATGTCGCTTTAACGCGTGCTAAAGAGAAAGTTTGGATTACCCA
TACCGGTGCGGGTTCGGCGTTTGTGCAAGAGTTGGTTAACGGCGATTATCCAATCGTCACTTCAAGATAA

Upstream 100 bases:

>100_bases
GAATACCGACAGAAGGCCATTTGTGCCTCATCCATCGCTACAATTTAACCGTCTAAAATGATATAAAACTGGAACTTCTA
TATCAAAAGGCGCACCTTCC

Downstream 100 bases:

>100_bases
AATCTATCCGTGATTTAGGTACAACAAAGGCGATACTGAGTATCGCCTTTGCTTTATTTGCTACTGACAGAGCTGCTAAT
CGAAATTAAGTGCGTTCTGC

Product: DNA helicase IV

Products: NA

Alternate protein names: 75 kDa helicase [H]

Number of amino acids: Translated: 689; Mature: 689

Protein sequence:

>689_residues
MQLTASNKAQFFIQNEYYQVEIKENSVLLSSVGSEEHIPFTIWNGKVSVKRGLLWSSLQFFAHEQDGKQQSWLVQGLSWP
HCRKFAVEAVRQYQDWHNTQCRKLAEYLPKWEDELYQLKHLPAYLSHSKVQTWVDQLNSELVEIKTSLVEAKMRMPKRMA
EIEPWLSETSQTLSERNHEWLENERPNWEVLFSRIESSPLNLSQQHAVLLNDDHNLVLAGAGSGKTSVLTARVAYLLQSH
QTQAEELLMLAFGRDAAKEMKERLVDKVGLAAEGVRVNTFHQLGLRILNQVEPQPVEISPLALDDNQRTAWCIDWLKKHW
MTPTNFKRWQKHLDKWPIAYLKGDDELGSHSENPKLIAWLDNQLSQLAAVGLTKKQVQEKLVNHQDYTRLNSELALCWPC
FSAWQKMLKETNQVDFPTMISRATDYVKKGKYISPWRFIMVDEYQDISPDRLALIEALCESTEKQAGATLFAVGDDWQAI
YQFAGADVDLTTGFQDRFTHSTVHHLDTTYRFNNQIGDVANTFVQENPSQLPKTLNSHKQRKQKSVHTAPSNQVEKILEQ
LNQQAKKTKSVLLLGRNHYHKPDLYDDWLKRFPKLEIRFMTCHASKGREADFVIILSVDEGQFPAKKKQAHIDGALSESK
DKYPYAEERRLFYVALTRAKEKVWITHTGAGSAFVQELVNGDYPIVTSR

Sequences:

>Translated_689_residues
MQLTASNKAQFFIQNEYYQVEIKENSVLLSSVGSEEHIPFTIWNGKVSVKRGLLWSSLQFFAHEQDGKQQSWLVQGLSWP
HCRKFAVEAVRQYQDWHNTQCRKLAEYLPKWEDELYQLKHLPAYLSHSKVQTWVDQLNSELVEIKTSLVEAKMRMPKRMA
EIEPWLSETSQTLSERNHEWLENERPNWEVLFSRIESSPLNLSQQHAVLLNDDHNLVLAGAGSGKTSVLTARVAYLLQSH
QTQAEELLMLAFGRDAAKEMKERLVDKVGLAAEGVRVNTFHQLGLRILNQVEPQPVEISPLALDDNQRTAWCIDWLKKHW
MTPTNFKRWQKHLDKWPIAYLKGDDELGSHSENPKLIAWLDNQLSQLAAVGLTKKQVQEKLVNHQDYTRLNSELALCWPC
FSAWQKMLKETNQVDFPTMISRATDYVKKGKYISPWRFIMVDEYQDISPDRLALIEALCESTEKQAGATLFAVGDDWQAI
YQFAGADVDLTTGFQDRFTHSTVHHLDTTYRFNNQIGDVANTFVQENPSQLPKTLNSHKQRKQKSVHTAPSNQVEKILEQ
LNQQAKKTKSVLLLGRNHYHKPDLYDDWLKRFPKLEIRFMTCHASKGREADFVIILSVDEGQFPAKKKQAHIDGALSESK
DKYPYAEERRLFYVALTRAKEKVWITHTGAGSAFVQELVNGDYPIVTSR
>Mature_689_residues
MQLTASNKAQFFIQNEYYQVEIKENSVLLSSVGSEEHIPFTIWNGKVSVKRGLLWSSLQFFAHEQDGKQQSWLVQGLSWP
HCRKFAVEAVRQYQDWHNTQCRKLAEYLPKWEDELYQLKHLPAYLSHSKVQTWVDQLNSELVEIKTSLVEAKMRMPKRMA
EIEPWLSETSQTLSERNHEWLENERPNWEVLFSRIESSPLNLSQQHAVLLNDDHNLVLAGAGSGKTSVLTARVAYLLQSH
QTQAEELLMLAFGRDAAKEMKERLVDKVGLAAEGVRVNTFHQLGLRILNQVEPQPVEISPLALDDNQRTAWCIDWLKKHW
MTPTNFKRWQKHLDKWPIAYLKGDDELGSHSENPKLIAWLDNQLSQLAAVGLTKKQVQEKLVNHQDYTRLNSELALCWPC
FSAWQKMLKETNQVDFPTMISRATDYVKKGKYISPWRFIMVDEYQDISPDRLALIEALCESTEKQAGATLFAVGDDWQAI
YQFAGADVDLTTGFQDRFTHSTVHHLDTTYRFNNQIGDVANTFVQENPSQLPKTLNSHKQRKQKSVHTAPSNQVEKILEQ
LNQQAKKTKSVLLLGRNHYHKPDLYDDWLKRFPKLEIRFMTCHASKGREADFVIILSVDEGQFPAKKKQAHIDGALSESK
DKYPYAEERRLFYVALTRAKEKVWITHTGAGSAFVQELVNGDYPIVTSR

Specific function: Helicase IV catalyzes the unwinding of duplex DNA in the 3' to 5' direction with respect to the bound single strand in a reaction that is dependent upon the hydrolysis of ATP [H]

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase ATP-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787196, Length=696, Percent_Identity=34.1954022988506, Blast_Score=355, Evalue=4e-99,
Organism=Escherichia coli, GI48994965, Length=344, Percent_Identity=28.4883720930233, Blast_Score=100, Evalue=4e-22,
Organism=Escherichia coli, GI2367296, Length=346, Percent_Identity=28.3236994219653, Blast_Score=95, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6322369, Length=310, Percent_Identity=26.7741935483871, Blast_Score=84, Evalue=6e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000212
- InterPro:   IPR014016
- InterPro:   IPR022161
- InterPro:   IPR003661 [H]

Pfam domain/function: PF12462 Nucleolin_N; PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 79571; Mature: 79571

Theoretical pI: Translated: 7.43; Mature: 7.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQLTASNKAQFFIQNEYYQVEIKENSVLLSSVGSEEHIPFTIWNGKVSVKRGLLWSSLQF
CCCCCCCCCEEEEECCEEEEEEECCCEEEECCCCCCCCCEEEECCEEEHHHHHHHHHHHH
FAHEQDGKQQSWLVQGLSWPHCRKFAVEAVRQYQDWHNTQCRKLAEYLPKWEDELYQLKH
HHHCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHH
LPAYLSHSKVQTWVDQLNSELVEIKTSLVEAKMRMPKRMAEIEPWLSETSQTLSERNHEW
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
LENERPNWEVLFSRIESSPLNLSQQHAVLLNDDHNLVLAGAGSGKTSVLTARVAYLLQSH
HHCCCCCHHHHHHHHCCCCCCCCCCCEEEEECCCCEEEEECCCCCHHHHHHHHHHHHHHH
QTQAEELLMLAFGRDAAKEMKERLVDKVGLAAEGVRVNTFHQLGLRILNQVEPQPVEISP
CHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHCCCCCCEEEE
LALDDNQRTAWCIDWLKKHWMTPTNFKRWQKHLDKWPIAYLKGDDELGSHSENPKLIAWL
EEECCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHCCCEEEEECCCCCCCCCCCCCEEEEH
DNQLSQLAAVGLTKKQVQEKLVNHQDYTRLNSELALCWPCFSAWQKMLKETNQVDFPTMI
HHHHHHHHHHCCCHHHHHHHHHCCHHHHHHCCCCEEEHHHHHHHHHHHHHCCCCCCHHHH
SRATDYVKKGKYISPWRFIMVDEYQDISPDRLALIEALCESTEKQAGATLFAVGDDWQAI
HHHHHHHHCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCEEEEECCCHHHH
YQFAGADVDLTTGFQDRFTHSTVHHLDTTYRFNNQIGDVANTFVQENPSQLPKTLNSHKQ
HHHHCCCEEEECCCHHHHHHHHHHHHHHHEEECCHHHHHHHHHHHCCHHHHHHHHHHHHH
RKQKSVHTAPSNQVEKILEQLNQQAKKTKSVLLLGRNHYHKPDLYDDWLKRFPKLEIRFM
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCHHHHHHHHCCCEEEEEE
TCHASKGREADFVIILSVDEGQFPAKKKQAHIDGALSESKDKYPYAEERRLFYVALTRAK
EEECCCCCCCCEEEEEEECCCCCCCHHHHHHHCCHHHCCCCCCCCHHHCEEEEEEEECCC
EKVWITHTGAGSAFVQELVNGDYPIVTSR
CEEEEEECCCCHHHHHHHHCCCCCEEECC
>Mature Secondary Structure
MQLTASNKAQFFIQNEYYQVEIKENSVLLSSVGSEEHIPFTIWNGKVSVKRGLLWSSLQF
CCCCCCCCCEEEEECCEEEEEEECCCEEEECCCCCCCCCEEEECCEEEHHHHHHHHHHHH
FAHEQDGKQQSWLVQGLSWPHCRKFAVEAVRQYQDWHNTQCRKLAEYLPKWEDELYQLKH
HHHCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHH
LPAYLSHSKVQTWVDQLNSELVEIKTSLVEAKMRMPKRMAEIEPWLSETSQTLSERNHEW
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
LENERPNWEVLFSRIESSPLNLSQQHAVLLNDDHNLVLAGAGSGKTSVLTARVAYLLQSH
HHCCCCCHHHHHHHHCCCCCCCCCCCEEEEECCCCEEEEECCCCCHHHHHHHHHHHHHHH
QTQAEELLMLAFGRDAAKEMKERLVDKVGLAAEGVRVNTFHQLGLRILNQVEPQPVEISP
CHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHCCCCCCEEEE
LALDDNQRTAWCIDWLKKHWMTPTNFKRWQKHLDKWPIAYLKGDDELGSHSENPKLIAWL
EEECCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHCCCEEEEECCCCCCCCCCCCCEEEEH
DNQLSQLAAVGLTKKQVQEKLVNHQDYTRLNSELALCWPCFSAWQKMLKETNQVDFPTMI
HHHHHHHHHHCCCHHHHHHHHHCCHHHHHHCCCCEEEHHHHHHHHHHHHHCCCCCCHHHH
SRATDYVKKGKYISPWRFIMVDEYQDISPDRLALIEALCESTEKQAGATLFAVGDDWQAI
HHHHHHHHCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCEEEEECCCHHHH
YQFAGADVDLTTGFQDRFTHSTVHHLDTTYRFNNQIGDVANTFVQENPSQLPKTLNSHKQ
HHHHCCCEEEECCCHHHHHHHHHHHHHHHEEECCHHHHHHHHHHHCCHHHHHHHHHHHHH
RKQKSVHTAPSNQVEKILEQLNQQAKKTKSVLLLGRNHYHKPDLYDDWLKRFPKLEIRFM
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCHHHHHHHHCCCEEEEEE
TCHASKGREADFVIILSVDEGQFPAKKKQAHIDGALSESKDKYPYAEERRLFYVALTRAK
EEECCCCCCCCEEEEEEECCCCCCCHHHHHHHCCHHHCCCCCCCCHHHCEEEEEEEECCC
EKVWITHTGAGSAFVQELVNGDYPIVTSR
CEEEEEECCCCHHHHHHHHCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2542273; 8905232; 9278503 [H]