Click here to switch to the map view.

The map label for this gene is cqsS

Identifier: 156977303

GI number: 156977303

Start: 1252155

End: 1254200

Strand: Reverse

Name: cqsS

Synonym: VIBHAR_06089

Alternate gene names: 156977303

Gene position: 1254200-1252155 (Counterclockwise)

Preceding gene: 156977307

Following gene: 156977300

Centisome position: 56.91

GC content: 43.94

Gene sequence:

>2046_bases
ATGGACGCGATTCGCAAAGTATATCAGTACGCAGAACCCAACCTTAGCCTCGTAGGATGGATGGGCTTCATAGGCTTTCC
TATTTACTACATCGTTTGGGAGTTCATGTTCCCGCAGCCATACGAAAACCTACCGCTTCGTATTCTTTGCTCAGTCTTAT
TCTTCGGCATCATTTATCGTAATCGTACGCCATTTGAGTGGCGTGGATTTTTACCCGCCTATTATCAAGTGGTCACAACG
CTCTGCTTACCTTGCTTCTTCTTTTACATGTTGCTGATGAACAATTGGTCGAATGTTTGGGTGATGTCGTTTATGTCGGC
GATTTTTTTGCACATACTGCTCGTGCATATCACATCGGTGATGTTTGTTCAGACATTTGTGGGAATTGGTCTTGCGACGT
TTTTTGCTTGGGTAGCACAGGGGTTTCACCTCGAACTCACCATGGATTGGACGCACGTACCGATCTTCTTGTTTATTTAT
CTCTTTGGTAACTTGTTCTACTTCCGAAACCAGGTAGAACATGAAGCAAAAGTCTCAATCGCAAAGTCTTTTGGGGCGGG
TATCGCCCATGAAATGCGAAACCCACTCAGCGGTTTATTAACTTCTATCGATGTCATTCAATCCGTGTTGCCAAATCCGA
AAGAGGGCAAGAAAGAGCAGTACACGTTAAGTGATGAAGATGTGACACTGTTACGAGAAGTCAGCAGTGACGCAATGAAG
ATCATTCATTCCGGAAACGAAACCATTGATTTGCTTCTTACTTCAATTGATGAGAACCGTGTTTCTCGCTCGACATTTAA
AAAACACTCTGCACAATCTGTCGTTGAGAGTGCGATTGAAAGTTTCAGTTACAAACGATCGACAGACCGTTTTGCTATCT
CCTTGGATGTAAGAAGTGAGTTCGATTTCTTGGGCAGCGATACATTGCTTAAGTATGTGATGTACAACTTGTTCAAGAAC
GCCTTCCACCACCGTAGTTCAGAAGACTTCCACATACACGTCACCATGTACAGTGATGAATTTGCCAACCAAATTGTGGT
GACTGATAACGGTTCGGGCATTGCGCCAGAAGTTCTTCAAAGTATTTTCCAAGATTTCTACACTACGGGTAAGTCAGGAA
ACTACGGCCTTGGACTGCCTTTCTGTAAGAAAGTGATGCGTTCATTTGGCGGTGATATTCGTTGCCAATCAGAAGTCGGC
GAGTGGTCTCAGTTCACGATGACTTTCCCTACGATTGGCTCAAGCGCAGTCAAAGAGATTAAGAGTGAGCTAACCAAGCT
AAAAACGATTTTGTTTGTGAGTGAGCAAAACATCTTAGTTTCGAAAGTGACGGACATTGCTCGTTTTATGAGATTCGAAC
TCACGGTTCTCGATGTTCCTGCGGTTCTTAAAAATAAAGAATACGAGTTCGAGTTTGATTTGATCCTGATCGACATGGAG
AGTTTAGACGCCAGTGGCAGCCATATAGATAAGGTTGAGTCGTTACTGTCGTTCACTGAAGCTCGCATTGTTTACATGTT
TGAGCACCATCCAATTCAAAGAGCGAGAAGCGTTTCTTTTGAACCTATTTGGGTAGAGACACAAGCCTGGCTTCTGAATA
CTCGAGCGACCATCGACCGTTTACTGTATGACGCTAATTATGTTGTTCCATCGATGCCAGCGAAACCGCTCGATTCAACA
AACAAGCGAACCATTATGGTGGTTGACGATAATGAGTCTTTACGCAAGTTTACGGCCATGCTGCTTGAGAAGCAGGGTTT
TGAAGTGATCCAAACTGAGGATGGGCTGCAGGCAATCAACGCATTGAATGAGAACAATGTTGACTTAATCCTAATGGACA
TCGAAATGCCGGTGATGGATGGTGTAGAAGCATCTCGTCAAATCAGAGGTTCAAATAAGGCCTACGCTTCGGTACCTATT
ATTGCGCATACTGGTGATAGCTCACCGATAACACTGGACAAGATTGGTTCATCTGGCATGTCGGATTTCATCGTTAAACC
TGCGGATAAGAACAGGTTGTTCGACAAGATTGCGAACTGGATTTAG

Upstream 100 bases:

>100_bases
GCTTATGTGCGATAACTAATATATAATTGAAAGACAAATACAAAGAAAACAATGCTAAACATAAAATTAGTAAGTGCTAG
GCTCAGTAGATAAAGTAAAT

Downstream 100 bases:

>100_bases
TTCTTGCTCAATAGGGTGACGAAAACGAATAGAGCTCAGCACTATGCTGAGCTCTTTGTATTCCGGCATTCACAAGCTTC
CATTCAAATGTGCCGCTTTC

Product: transmembrane sensor histidine kinase

Products: NA

Alternate protein names: Cholerae quorum-sensing sensor

Number of amino acids: Translated: 681; Mature: 681

Protein sequence:

>681_residues
MDAIRKVYQYAEPNLSLVGWMGFIGFPIYYIVWEFMFPQPYENLPLRILCSVLFFGIIYRNRTPFEWRGFLPAYYQVVTT
LCLPCFFFYMLLMNNWSNVWVMSFMSAIFLHILLVHITSVMFVQTFVGIGLATFFAWVAQGFHLELTMDWTHVPIFLFIY
LFGNLFYFRNQVEHEAKVSIAKSFGAGIAHEMRNPLSGLLTSIDVIQSVLPNPKEGKKEQYTLSDEDVTLLREVSSDAMK
IIHSGNETIDLLLTSIDENRVSRSTFKKHSAQSVVESAIESFSYKRSTDRFAISLDVRSEFDFLGSDTLLKYVMYNLFKN
AFHHRSSEDFHIHVTMYSDEFANQIVVTDNGSGIAPEVLQSIFQDFYTTGKSGNYGLGLPFCKKVMRSFGGDIRCQSEVG
EWSQFTMTFPTIGSSAVKEIKSELTKLKTILFVSEQNILVSKVTDIARFMRFELTVLDVPAVLKNKEYEFEFDLILIDME
SLDASGSHIDKVESLLSFTEARIVYMFEHHPIQRARSVSFEPIWVETQAWLLNTRATIDRLLYDANYVVPSMPAKPLDST
NKRTIMVVDDNESLRKFTAMLLEKQGFEVIQTEDGLQAINALNENNVDLILMDIEMPVMDGVEASRQIRGSNKAYASVPI
IAHTGDSSPITLDKIGSSGMSDFIVKPADKNRLFDKIANWI

Sequences:

>Translated_681_residues
MDAIRKVYQYAEPNLSLVGWMGFIGFPIYYIVWEFMFPQPYENLPLRILCSVLFFGIIYRNRTPFEWRGFLPAYYQVVTT
LCLPCFFFYMLLMNNWSNVWVMSFMSAIFLHILLVHITSVMFVQTFVGIGLATFFAWVAQGFHLELTMDWTHVPIFLFIY
LFGNLFYFRNQVEHEAKVSIAKSFGAGIAHEMRNPLSGLLTSIDVIQSVLPNPKEGKKEQYTLSDEDVTLLREVSSDAMK
IIHSGNETIDLLLTSIDENRVSRSTFKKHSAQSVVESAIESFSYKRSTDRFAISLDVRSEFDFLGSDTLLKYVMYNLFKN
AFHHRSSEDFHIHVTMYSDEFANQIVVTDNGSGIAPEVLQSIFQDFYTTGKSGNYGLGLPFCKKVMRSFGGDIRCQSEVG
EWSQFTMTFPTIGSSAVKEIKSELTKLKTILFVSEQNILVSKVTDIARFMRFELTVLDVPAVLKNKEYEFEFDLILIDME
SLDASGSHIDKVESLLSFTEARIVYMFEHHPIQRARSVSFEPIWVETQAWLLNTRATIDRLLYDANYVVPSMPAKPLDST
NKRTIMVVDDNESLRKFTAMLLEKQGFEVIQTEDGLQAINALNENNVDLILMDIEMPVMDGVEASRQIRGSNKAYASVPI
IAHTGDSSPITLDKIGSSGMSDFIVKPADKNRLFDKIANWI
>Mature_681_residues
MDAIRKVYQYAEPNLSLVGWMGFIGFPIYYIVWEFMFPQPYENLPLRILCSVLFFGIIYRNRTPFEWRGFLPAYYQVVTT
LCLPCFFFYMLLMNNWSNVWVMSFMSAIFLHILLVHITSVMFVQTFVGIGLATFFAWVAQGFHLELTMDWTHVPIFLFIY
LFGNLFYFRNQVEHEAKVSIAKSFGAGIAHEMRNPLSGLLTSIDVIQSVLPNPKEGKKEQYTLSDEDVTLLREVSSDAMK
IIHSGNETIDLLLTSIDENRVSRSTFKKHSAQSVVESAIESFSYKRSTDRFAISLDVRSEFDFLGSDTLLKYVMYNLFKN
AFHHRSSEDFHIHVTMYSDEFANQIVVTDNGSGIAPEVLQSIFQDFYTTGKSGNYGLGLPFCKKVMRSFGGDIRCQSEVG
EWSQFTMTFPTIGSSAVKEIKSELTKLKTILFVSEQNILVSKVTDIARFMRFELTVLDVPAVLKNKEYEFEFDLILIDME
SLDASGSHIDKVESLLSFTEARIVYMFEHHPIQRARSVSFEPIWVETQAWLLNTRATIDRLLYDANYVVPSMPAKPLDST
NKRTIMVVDDNESLRKFTAMLLEKQGFEVIQTEDGLQAINALNENNVDLILMDIEMPVMDGVEASRQIRGSNKAYASVPI
IAHTGDSSPITLDKIGSSGMSDFIVKPADKNRLFDKIANWI

Specific function: Senses the quorum-sensing autoinducer CAI-1 ((S)-3- hydroxytridecan-4-one) which probably functions as an intragenus signal. The sensory signal is then relayed to luxU and luxO

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain

Homologues:

Organism=Escherichia coli, GI145693157, Length=510, Percent_Identity=25.0980392156863, Blast_Score=110, Evalue=4e-25,
Organism=Escherichia coli, GI1789149, Length=526, Percent_Identity=23.0038022813688, Blast_Score=90, Evalue=5e-19,
Organism=Escherichia coli, GI1788549, Length=265, Percent_Identity=26.7924528301887, Blast_Score=80, Evalue=4e-16,
Organism=Escherichia coli, GI1790436, Length=254, Percent_Identity=27.5590551181102, Blast_Score=75, Evalue=9e-15,
Organism=Escherichia coli, GI1786600, Length=285, Percent_Identity=24.9122807017544, Blast_Score=69, Evalue=8e-13,
Organism=Escherichia coli, GI87081816, Length=283, Percent_Identity=26.8551236749117, Blast_Score=68, Evalue=2e-12,
Organism=Escherichia coli, GI1788713, Length=120, Percent_Identity=30, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1788191, Length=121, Percent_Identity=29.7520661157025, Blast_Score=64, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CQSS_VIBHB (A7N6S2)

Other databases:

- EMBL:   AY625893
- EMBL:   CP000790
- RefSeq:   YP_001448209.1
- ProteinModelPortal:   A7N6S2
- STRING:   A7N6S2
- GeneID:   5556966
- GenomeReviews:   CP000790_GR
- KEGG:   vha:VIBHAR_06089
- NMPDR:   fig|338187.4.peg.3584
- eggNOG:   COG0642
- HOGENOM:   HBG625184
- OMA:   AIFLHIL
- ProtClustDB:   CLSK869633
- InterPro:   IPR003594
- InterPro:   IPR011006
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082
- InterPro:   IPR001789
- Gene3D:   G3DSA:3.30.565.10
- PRINTS:   PR00344
- SMART:   SM00387
- SMART:   SM00388
- SMART:   SM00448

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00072 Response_reg; SSF55874 ATP_bd_ATPase; SSF52172 CheY_like; SSF47384 His_kin_homodim

EC number: =2.7.13.3

Molecular weight: Translated: 77799; Mature: 77799

Theoretical pI: Translated: 5.30; Mature: 5.30

Prosite motif: PS50109 HIS_KIN; PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x1952672c)-; HASH(0x19ec9f3c)-; HASH(0x1a4d401c)-; HASH(0x17703d54)-;

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCEECCCCHHHHHHHHHCCH
>Mature Secondary Structure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CCCEECCCCHHHHHHHHHCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA