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The map label for this gene is pcbC [H]

Identifier: 156977022

GI number: 156977022

Start: 996591

End: 997430

Strand: Reverse

Name: pcbC [H]

Synonym: VIBHAR_05807

Alternate gene names: 156977022

Gene position: 997430-996591 (Counterclockwise)

Preceding gene: 156977026

Following gene: 156977021

Centisome position: 45.26

GC content: 47.02

Gene sequence:

>840_bases
ATGAAACTGGAAACTGTCGATTATCTTGCTGACGACGCTGCTCAACAGTTCGTCACCTCACTGCGCGAAACAGGCTTTGG
TGTCCTTAAAAACCACCCAATCCCTCAAGAGCTGGTGGAGTCTATTTACAAAAACTGGTACGAGTTTTTCTGCTCTGAGC
AAAAGAACGACTTCCACTTCAATGTTGAAACACAAGACGGTTATTTTCCACCATCAGTTTCTGAAGTCGCGAAAGGCCAC
TCAGTAAAAGATATTAAAGAGTACTTTCATGTATACCCTTGGGGACAAATGCCTGAGCAGCTAAAAGCGGAAATCATGGA
ATACTATGACCGTGCTAACGCGTTTGCTCAAGAACTGCTTGGCTGGGTAGAAGAATACGCACCAAAAGAGGTACAAGAGA
AGTTCTCTATCGCGTTGTCTGAAATGATCAACAACAGCGATAAAACACTTCTACGCGTTCTGCATTACCCACCGATGACT
GGCGAAGAAGAGCCAGGCGCAATTCGTGCGGCTGCCCATGAAGATATCAACCTTCTGACGGTACTGCCAGCAGCGAACGA
GCCGGGTCTTCAGGTTAAGAGCAAAGAAGGTGATTGGCTAGATGTGCCATGTGATTTCGGTAACCTAATCATCAACATTG
GTGACATGCTGCAAGAAGCATCGGGTGGTTACTTCCCATCGACGACTCACCGCGTTATCAACCCAACAGGCGGTCGCCAA
GAAAAATCTCGTATTTCTTTGCCACTGTTCCTGCACCCAAAACCGGATACTGTGCTTTCTGAGCGTTACACCGCACACAG
TTACTTGATGGAGCGTCTGAGAGAGCTGGGCGTTATTTAA

Upstream 100 bases:

>100_bases
ACAATAAAACGTTTGCGCACAAACATTTCATTAAAATTTGATTTACAACATGGCCATCCATAGAATTCAACGCTCAGTCT
AATATAAAAGAAGTAATGTC

Downstream 100 bases:

>100_bases
GTTAACGAGATCCTCCTTCAAAAGGCTGACGGTTTATGACTGTCAGCCTTTTTGTGTTTATGGTACAAATTGACTAGCTT
TAATAACAGGTACTTAAAAC

Product: isopenicillin N synthase

Products: NA

Alternate protein names: IPNS [H]

Number of amino acids: Translated: 279; Mature: 279

Protein sequence:

>279_residues
MKLETVDYLADDAAQQFVTSLRETGFGVLKNHPIPQELVESIYKNWYEFFCSEQKNDFHFNVETQDGYFPPSVSEVAKGH
SVKDIKEYFHVYPWGQMPEQLKAEIMEYYDRANAFAQELLGWVEEYAPKEVQEKFSIALSEMINNSDKTLLRVLHYPPMT
GEEEPGAIRAAAHEDINLLTVLPAANEPGLQVKSKEGDWLDVPCDFGNLIINIGDMLQEASGGYFPSTTHRVINPTGGRQ
EKSRISLPLFLHPKPDTVLSERYTAHSYLMERLRELGVI

Sequences:

>Translated_279_residues
MKLETVDYLADDAAQQFVTSLRETGFGVLKNHPIPQELVESIYKNWYEFFCSEQKNDFHFNVETQDGYFPPSVSEVAKGH
SVKDIKEYFHVYPWGQMPEQLKAEIMEYYDRANAFAQELLGWVEEYAPKEVQEKFSIALSEMINNSDKTLLRVLHYPPMT
GEEEPGAIRAAAHEDINLLTVLPAANEPGLQVKSKEGDWLDVPCDFGNLIINIGDMLQEASGGYFPSTTHRVINPTGGRQ
EKSRISLPLFLHPKPDTVLSERYTAHSYLMERLRELGVI
>Mature_279_residues
MKLETVDYLADDAAQQFVTSLRETGFGVLKNHPIPQELVESIYKNWYEFFCSEQKNDFHFNVETQDGYFPPSVSEVAKGH
SVKDIKEYFHVYPWGQMPEQLKAEIMEYYDRANAFAQELLGWVEEYAPKEVQEKFSIALSEMINNSDKTLLRVLHYPPMT
GEEEPGAIRAAAHEDINLLTVLPAANEPGLQVKSKEGDWLDVPCDFGNLIINIGDMLQEASGGYFPSTTHRVINPTGGRQ
EKSRISLPLFLHPKPDTVLSERYTAHSYLMERLRELGVI

Specific function: Removes, in the presence of oxygen, 4 hydrogen atoms from delta-L-(alpha-aminoadipyl)-L-cysteinyl-D-valine (ACV) to form the azetidinone and thiazolidine rings of isopenicillin [H]

COG id: COG3491

COG function: function code R; Isopenicillin N synthase and related dioxygenases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Fe2OG dioxygenase domain [H]

Homologues:

Organism=Drosophila melanogaster, GI85816264, Length=269, Percent_Identity=23.7918215613383, Blast_Score=67, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002057
- InterPro:   IPR002283
- InterPro:   IPR005123 [H]

Pfam domain/function: PF03171 2OG-FeII_Oxy [H]

EC number: =1.21.3.1 [H]

Molecular weight: Translated: 31818; Mature: 31818

Theoretical pI: Translated: 4.62; Mature: 4.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLETVDYLADDAAQQFVTSLRETGFGVLKNHPIPQELVESIYKNWYEFFCSEQKNDFHF
CCCCHHHHHHHHHHHHHHHHHHHCCCCHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEE
NVETQDGYFPPSVSEVAKGHSVKDIKEYFHVYPWGQMPEQLKAEIMEYYDRANAFAQELL
EEECCCCCCCCCHHHHHCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
GWVEEYAPKEVQEKFSIALSEMINNSDKTLLRVLHYPPMTGEEEPGAIRAAAHEDINLLT
HHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCEEHHCCCCCEEEE
VLPAANEPGLQVKSKEGDWLDVPCDFGNLIINIGDMLQEASGGYFPSTTHRVINPTGGRQ
EEECCCCCCCEEECCCCCEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCC
EKSRISLPLFLHPKPDTVLSERYTAHSYLMERLRELGVI
CHHHCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKLETVDYLADDAAQQFVTSLRETGFGVLKNHPIPQELVESIYKNWYEFFCSEQKNDFHF
CCCCHHHHHHHHHHHHHHHHHHHCCCCHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEE
NVETQDGYFPPSVSEVAKGHSVKDIKEYFHVYPWGQMPEQLKAEIMEYYDRANAFAQELL
EEECCCCCCCCCHHHHHCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
GWVEEYAPKEVQEKFSIALSEMINNSDKTLLRVLHYPPMTGEEEPGAIRAAAHEDINLLT
HHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCEEHHCCCCCEEEE
VLPAANEPGLQVKSKEGDWLDVPCDFGNLIINIGDMLQEASGGYFPSTTHRVINPTGGRQ
EEECCCCCCCEEECCCCCEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCC
EKSRISLPLFLHPKPDTVLSERYTAHSYLMERLRELGVI
CHHHCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1901702 [H]