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The map label for this gene is bkdB [H]

Identifier: 156976778

GI number: 156976778

Start: 757309

End: 758451

Strand: Reverse

Name: bkdB [H]

Synonym: VIBHAR_05553

Alternate gene names: 156976778

Gene position: 758451-757309 (Counterclockwise)

Preceding gene: 156976779

Following gene: 156976775

Centisome position: 34.41

GC content: 50.31

Gene sequence:

>1143_bases
ATGAAGACGTTTAACTTACCCGATCTCGGCGAAGGTCTCGCTGAGTCAGAAATTGTGAAATGGCATATTAGTGTTGGCGA
TATGGTCCAACTCGATCAAATAGTACTTACCGTGGAAACCGCAAAAGCGACAGTGGATGTCCCTGCTCCTTATGGTGGTC
GCATCGTCAGTCGTCACGGTGAAGAAGGTGATGTGATTAACATCGGTGCTCTACTTCTGGAAATCGATGAAACTGGGGCA
GAACAAGCAGCTACAGAACAAAAGCAAACGGCTGATGCTGCAACCGTGGTGGGTAATGTCTCCCAGCACGCACATAGCGT
GAATGTCGATGACTTCTGGATTGGCGGTGAACATAACCCCTCTCCCGATAATCTCATCAGCGCTCTCCCTTCGGCGCGTT
TGTTAGCGAATAAACTAGGAGTCGATTTAGCGTCGGTGAAAGGTAGCGGCCCCGACGGTTTAATCCTTGATTCAGATATC
TATGACGAAGCTGGCAAACAGCGCCCAGGAACAGAGGTGCTGAAAGGTGCACGTCGAACCATGGTGTCGACAATGGCGGA
ATCTCACCATAATGTCGCAGCAGTAACCATTACTGAGGAAGCGGTCTTAGATGGTTGGAAGCAAGGCGAAGACATCAGTA
TTCGTCTCGTCCAAGCCATTGTGCATGCTTGTCAGGAAGAACCCGCCATGAATGCATGGTTTGATGCGGAAACCATGACG
CGTTGTGTCCACAGTACAGTCAACATCGGTATTGCGGTCGACAGCCGACACGGTTTGTACGTGCCTGTTCTTCGACACGC
TGATGAGTATGAGCCGCAAGAGGTGCGCCGTTGGTTAGATCAAACCGTACAAGGTATACGCGAACGTAAGATTGGTCGCG
ATCAGTTACAGCACGCCACCATTACGCTTTCAAATTTTGGCGCAATTGCAGGTATCTACGCAACGCCTGTTGTAACTCCT
CCGCAAGTTGCGATTGTCGGTGCGGGTCGAATCATTGAGAAGGTCGTAATTCGAGACGGGCAAGCTGTAGCAGTGAAAGC
CATGCCGCTGTCGATTACGTTTGACCACCGAGCATGTACTGGTGGTGAAGCAGCAAGGTTCACAAAAGTACTGGCAGAGC
ACTTGAGAAAACCGAGTGTTTAG

Upstream 100 bases:

>100_bases
GTATGGATACCATTATGCCGTACTACCGCAACGAAGATTATTACATGGTTCAGGAAGAAGACATTGTACTCGCCGCGCGC
GAACTCGTGGAGGATTGGAA

Downstream 100 bases:

>100_bases
AGAACGCTTGTCACAAATAATAAAGGGCTTCCGATTTGAAGCCCTTTTGTTTTGAAGATGAATTTAAATGAGAGATTCCC
TACTCGTTCATTCTTCACTC

Product: branched-chain alpha-keto acid dehydrogenase subunit E2

Products: NA

Alternate protein names: Branched-chain alpha-keto acid dehydrogenase complex component E2; BCKAD-E2; BCKADE2; Dihydrolipoamide acetyltransferase component of branched-chain alpha-keto acid dehydrogenase complex; Dihydrolipoamide branched chain transacylase; Dihydrolipoyllysine-residue (2-methylpropanoyl)transferase [H]

Number of amino acids: Translated: 380; Mature: 380

Protein sequence:

>380_residues
MKTFNLPDLGEGLAESEIVKWHISVGDMVQLDQIVLTVETAKATVDVPAPYGGRIVSRHGEEGDVINIGALLLEIDETGA
EQAATEQKQTADAATVVGNVSQHAHSVNVDDFWIGGEHNPSPDNLISALPSARLLANKLGVDLASVKGSGPDGLILDSDI
YDEAGKQRPGTEVLKGARRTMVSTMAESHHNVAAVTITEEAVLDGWKQGEDISIRLVQAIVHACQEEPAMNAWFDAETMT
RCVHSTVNIGIAVDSRHGLYVPVLRHADEYEPQEVRRWLDQTVQGIRERKIGRDQLQHATITLSNFGAIAGIYATPVVTP
PQVAIVGAGRIIEKVVIRDGQAVAVKAMPLSITFDHRACTGGEAARFTKVLAEHLRKPSV

Sequences:

>Translated_380_residues
MKTFNLPDLGEGLAESEIVKWHISVGDMVQLDQIVLTVETAKATVDVPAPYGGRIVSRHGEEGDVINIGALLLEIDETGA
EQAATEQKQTADAATVVGNVSQHAHSVNVDDFWIGGEHNPSPDNLISALPSARLLANKLGVDLASVKGSGPDGLILDSDI
YDEAGKQRPGTEVLKGARRTMVSTMAESHHNVAAVTITEEAVLDGWKQGEDISIRLVQAIVHACQEEPAMNAWFDAETMT
RCVHSTVNIGIAVDSRHGLYVPVLRHADEYEPQEVRRWLDQTVQGIRERKIGRDQLQHATITLSNFGAIAGIYATPVVTP
PQVAIVGAGRIIEKVVIRDGQAVAVKAMPLSITFDHRACTGGEAARFTKVLAEHLRKPSV
>Mature_380_residues
MKTFNLPDLGEGLAESEIVKWHISVGDMVQLDQIVLTVETAKATVDVPAPYGGRIVSRHGEEGDVINIGALLLEIDETGA
EQAATEQKQTADAATVVGNVSQHAHSVNVDDFWIGGEHNPSPDNLISALPSARLLANKLGVDLASVKGSGPDGLILDSDI
YDEAGKQRPGTEVLKGARRTMVSTMAESHHNVAAVTITEEAVLDGWKQGEDISIRLVQAIVHACQEEPAMNAWFDAETMT
RCVHSTVNIGIAVDSRHGLYVPVLRHADEYEPQEVRRWLDQTVQGIRERKIGRDQLQHATITLSNFGAIAGIYATPVVTP
PQVAIVGAGRIIEKVVIRDGQAVAVKAMPLSITFDHRACTGGEAARFTKVLAEHLRKPSV

Specific function: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of three enzymatic components:branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltran

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI110671329, Length=430, Percent_Identity=25.8139534883721, Blast_Score=116, Evalue=3e-26,
Organism=Homo sapiens, GI19923748, Length=165, Percent_Identity=29.0909090909091, Blast_Score=89, Evalue=5e-18,
Organism=Escherichia coli, GI1786305, Length=421, Percent_Identity=26.3657957244656, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI1786946, Length=410, Percent_Identity=24.1463414634146, Blast_Score=113, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17537937, Length=428, Percent_Identity=26.6355140186916, Blast_Score=131, Evalue=5e-31,
Organism=Caenorhabditis elegans, GI25146366, Length=165, Percent_Identity=30.3030303030303, Blast_Score=89, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17560088, Length=427, Percent_Identity=26.2295081967213, Blast_Score=88, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI17538894, Length=177, Percent_Identity=27.683615819209, Blast_Score=66, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6320352, Length=405, Percent_Identity=22.962962962963, Blast_Score=102, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6324258, Length=169, Percent_Identity=27.2189349112426, Blast_Score=65, Evalue=1e-11,
Organism=Drosophila melanogaster, GI18859875, Length=426, Percent_Identity=25.5868544600939, Blast_Score=122, Evalue=4e-28,
Organism=Drosophila melanogaster, GI24645909, Length=165, Percent_Identity=27.8787878787879, Blast_Score=79, Evalue=5e-15,
Organism=Drosophila melanogaster, GI24582497, Length=179, Percent_Identity=26.2569832402235, Blast_Score=65, Evalue=6e-11,
Organism=Drosophila melanogaster, GI20129315, Length=180, Percent_Identity=26.6666666666667, Blast_Score=65, Evalue=1e-10,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 912 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.168 [H]

Molecular weight: Translated: 40918; Mature: 40918

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTFNLPDLGEGLAESEIVKWHISVGDMVQLDQIVLTVETAKATVDVPAPYGGRIVSRHG
CCCCCCCCCCCCCCCCCEEEEEECCCCCEEECEEEEEEEECCEEEECCCCCCCEEEECCC
EEGDVINIGALLLEIDETGAEQAATEQKQTADAATVVGNVSQHAHSVNVDDFWIGGEHNP
CCCCEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCHHHHHCCCCCCEEEECCCCCC
SPDNLISALPSARLLANKLGVDLASVKGSGPDGLILDSDIYDEAGKQRPGTEVLKGARRT
CHHHHHHHCCHHHHHHHHHCCEEEECCCCCCCCEEECCHHHHHHCCCCCCHHHHHHHHHH
MVSTMAESHHNVAAVTITEEAVLDGWKQGEDISIRLVQAIVHACQEEPAMNAWFDAETMT
HHHHHHHCCCCEEEEEEEHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEECHHHHH
RCVHSTVNIGIAVDSRHGLYVPVLRHADEYEPQEVRRWLDQTVQGIRERKIGRDQLQHAT
HHHHHHEEEEEEEECCCCEEEHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHEE
ITLSNFGAIAGIYATPVVTPPQVAIVGAGRIIEKVVIRDGQAVAVKAMPLSITFDHRACT
EEECCCCCHHHHHCCCCCCCCCEEEEECHHHHHHHHHCCCCEEEEEEEEEEEEECCCCCC
GGEAARFTKVLAEHLRKPSV
CCHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKTFNLPDLGEGLAESEIVKWHISVGDMVQLDQIVLTVETAKATVDVPAPYGGRIVSRHG
CCCCCCCCCCCCCCCCCEEEEEECCCCCEEECEEEEEEEECCEEEECCCCCCCEEEECCC
EEGDVINIGALLLEIDETGAEQAATEQKQTADAATVVGNVSQHAHSVNVDDFWIGGEHNP
CCCCEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCHHHHHCCCCCCEEEECCCCCC
SPDNLISALPSARLLANKLGVDLASVKGSGPDGLILDSDIYDEAGKQRPGTEVLKGARRT
CHHHHHHHCCHHHHHHHHHCCEEEECCCCCCCCEEECCHHHHHHCCCCCCHHHHHHHHHH
MVSTMAESHHNVAAVTITEEAVLDGWKQGEDISIRLVQAIVHACQEEPAMNAWFDAETMT
HHHHHHHCCCCEEEEEEEHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEECHHHHH
RCVHSTVNIGIAVDSRHGLYVPVLRHADEYEPQEVRRWLDQTVQGIRERKIGRDQLQHAT
HHHHHHEEEEEEEECCCCEEEHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHEE
ITLSNFGAIAGIYATPVVTPPQVAIVGAGRIIEKVVIRDGQAVAVKAMPLSITFDHRACT
EEECCCCCHHHHHCCCCCCCCCEEEEECHHHHHHHHHCCCCEEEEEEEEEEEEECCCCCC
GGEAARFTKVLAEHLRKPSV
CCHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3046941 [H]