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The map label for this gene is apbE [H]

Identifier: 156976765

GI number: 156976765

Start: 746819

End: 747790

Strand: Direct

Name: apbE [H]

Synonym: VIBHAR_05540

Alternate gene names: 156976765

Gene position: 746819-747790 (Clockwise)

Preceding gene: 156976764

Following gene: 156976766

Centisome position: 33.88

GC content: 45.47

Gene sequence:

>972_bases
ATGAGCACGAATAAATACAGCGCCAGATTTCAGATGATGGGCACCTTTATCGATGTGGTGGTGTATCACTCGAAGGGTGA
GCAACTTATAAGAGAAGCTTACGCGCAACTCGCCGATTACGCAGAGCGATTTACGGTCAATCAACCCGACTCTGAATTGA
TGCGTGTCAATCAAAATGCGGGCATAGCACCTTTGGTGGTAGCAAATGACCTATTTAACCTGATTAAGCTTGCTAAACAG
CACAGCGAAGACACAAGAAATCCTTTTAACGTTGCAGTTGGTCCGCTTGTAAAAGCTTGGAGAATTGGTTTTAAGGATGC
CAAAGTGCCGTCTCATGATGTGATTACCGACAAGTTATCGTTGGTCGATCCTACCAAGATCATTCTGAGCGAAGATGACC
ACTCGGTGTTCTTAAGTCAAAAGGGGATGGAGATAGACTTGGGAGCGATAGCTAAAGGTTACTTCGCTGATCAGGTAAAG
AACAAACTGGTTGAGGCTGGCGTGCAAAGTGGTTTTATCAGTTTAGGTGGAAACGTACTGACCATCGGTCATTCGCCAGA
TAACAGCAACAAAGCATGGAATGTGGGCATTCAGAACCCATTATCACAGCGCGGTGATGTCGTTCGCATCGTACCTTTAC
AGGGCATGTCGATGGTGACATCGGGTATCAATGAGCGATTTTTTGAATCAAATGGGCAGCGTTATCATCACTTGTTGGAT
GCCAAAACGGGGATACCAATTTCAACCGATATCGCTAGTTTGACGATTATCTCTCGGCTGTCTGTCGATGGTGAAATTTG
GAGTACCGCAGGCTTTTTACCAAGCGTAGAAGAAGCGATGTCGTACCTAAATCAGCAACATTGCATCGAGGCTGTGGTTG
TTTCAAAACAAGGAGAAGTGCACGTCACACGGGGGCTGATGGATTATAATGGCTGTATTTTATTGCGAGAAGAGCCTGCA
CTTACAGAGTAA

Upstream 100 bases:

>100_bases
TTTAGGTACTCATTCAAGAGCAAAACAGAGCAGGATAAGTAATAATTCAGAGGGGGGCTATGCTTCCCTCTTTTTCCGTA
TTTAGCGTTTGGAGAAATCG

Downstream 100 bases:

>100_bases
GATGAGGCAGAAATTCAATCTTGCCTCATATCATCAAGTTATCGCGCTAGCGTTAATCATCCTCACCGCATTTAGCGCAC
TCTCCGTACGTGCGTTCGCC

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 323; Mature: 322

Protein sequence:

>323_residues
MSTNKYSARFQMMGTFIDVVVYHSKGEQLIREAYAQLADYAERFTVNQPDSELMRVNQNAGIAPLVVANDLFNLIKLAKQ
HSEDTRNPFNVAVGPLVKAWRIGFKDAKVPSHDVITDKLSLVDPTKIILSEDDHSVFLSQKGMEIDLGAIAKGYFADQVK
NKLVEAGVQSGFISLGGNVLTIGHSPDNSNKAWNVGIQNPLSQRGDVVRIVPLQGMSMVTSGINERFFESNGQRYHHLLD
AKTGIPISTDIASLTIISRLSVDGEIWSTAGFLPSVEEAMSYLNQQHCIEAVVVSKQGEVHVTRGLMDYNGCILLREEPA
LTE

Sequences:

>Translated_323_residues
MSTNKYSARFQMMGTFIDVVVYHSKGEQLIREAYAQLADYAERFTVNQPDSELMRVNQNAGIAPLVVANDLFNLIKLAKQ
HSEDTRNPFNVAVGPLVKAWRIGFKDAKVPSHDVITDKLSLVDPTKIILSEDDHSVFLSQKGMEIDLGAIAKGYFADQVK
NKLVEAGVQSGFISLGGNVLTIGHSPDNSNKAWNVGIQNPLSQRGDVVRIVPLQGMSMVTSGINERFFESNGQRYHHLLD
AKTGIPISTDIASLTIISRLSVDGEIWSTAGFLPSVEEAMSYLNQQHCIEAVVVSKQGEVHVTRGLMDYNGCILLREEPA
LTE
>Mature_322_residues
STNKYSARFQMMGTFIDVVVYHSKGEQLIREAYAQLADYAERFTVNQPDSELMRVNQNAGIAPLVVANDLFNLIKLAKQH
SEDTRNPFNVAVGPLVKAWRIGFKDAKVPSHDVITDKLSLVDPTKIILSEDDHSVFLSQKGMEIDLGAIAKGYFADQVKN
KLVEAGVQSGFISLGGNVLTIGHSPDNSNKAWNVGIQNPLSQRGDVVRIVPLQGMSMVTSGINERFFESNGQRYHHLLDA
KTGIPISTDIASLTIISRLSVDGEIWSTAGFLPSVEEAMSYLNQQHCIEAVVVSKQGEVHVTRGLMDYNGCILLREEPAL
TE

Specific function: Involved in the conversion of aminoimidazole ribotide (AIR), a purine intermediate, to the 4-amino-5-hydroxymethyl-2- methyl pyrimidine (HMP) moiety of thiamine [H]

COG id: COG1477

COG function: function code H; Membrane-associated lipoprotein involved in thiamine biosynthesis

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ApbE family [H]

Homologues:

Organism=Escherichia coli, GI1788543, Length=296, Percent_Identity=22.972972972973, Blast_Score=104, Evalue=9e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003374 [H]

Pfam domain/function: PF02424 ApbE [H]

EC number: NA

Molecular weight: Translated: 35560; Mature: 35429

Theoretical pI: Translated: 5.82; Mature: 5.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTNKYSARFQMMGTFIDVVVYHSKGEQLIREAYAQLADYAERFTVNQPDSELMRVNQNA
CCCCCHHHHHHHHHHHHEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHEECCCC
GIAPLVVANDLFNLIKLAKQHSEDTRNPFNVAVGPLVKAWRIGFKDAKVPSHDVITDKLS
CCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHC
LVDPTKIILSEDDHSVFLSQKGMEIDLGAIAKGYFADQVKNKLVEAGVQSGFISLGGNVL
CCCCEEEEEECCCCEEEEECCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCEE
TIGHSPDNSNKAWNVGIQNPLSQRGDVVRIVPLQGMSMVTSGINERFFESNGQRYHHLLD
EEECCCCCCCCEEECCCCCCHHHCCCEEEEEECCCHHHHHHHHHHHHHHCCCCHHHHHHH
AKTGIPISTDIASLTIISRLSVDGEIWSTAGFLPSVEEAMSYLNQQHCIEAVVVSKQGEV
CCCCCCCCCCHHHHHHHHHHCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHEECCCCCE
HVTRGLMDYNGCILLREEPALTE
EEEECEECCCCEEEEECCCCCCC
>Mature Secondary Structure 
STNKYSARFQMMGTFIDVVVYHSKGEQLIREAYAQLADYAERFTVNQPDSELMRVNQNA
CCCCHHHHHHHHHHHHEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHEECCCC
GIAPLVVANDLFNLIKLAKQHSEDTRNPFNVAVGPLVKAWRIGFKDAKVPSHDVITDKLS
CCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHC
LVDPTKIILSEDDHSVFLSQKGMEIDLGAIAKGYFADQVKNKLVEAGVQSGFISLGGNVL
CCCCEEEEEECCCCEEEEECCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCEE
TIGHSPDNSNKAWNVGIQNPLSQRGDVVRIVPLQGMSMVTSGINERFFESNGQRYHHLLD
EEECCCCCCCCEEECCCCCCHHHCCCEEEEEECCCHHHHHHHHHHHHHHCCCCHHHHHHH
AKTGIPISTDIASLTIISRLSVDGEIWSTAGFLPSVEEAMSYLNQQHCIEAVVVSKQGEV
CCCCCCCCCCHHHHHHHHHHCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHEECCCCCE
HVTRGLMDYNGCILLREEPALTE
EEEECEECCCCEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9665876 [H]