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The map label for this gene is opuD [H]

Identifier: 156976728

GI number: 156976728

Start: 713120

End: 714145

Strand: Direct

Name: opuD [H]

Synonym: VIBHAR_05503

Alternate gene names: 156976728

Gene position: 713120-714145 (Clockwise)

Preceding gene: 156976727

Following gene: 156976731

Centisome position: 32.36

GC content: 47.95

Gene sequence:

>1026_bases
ATGTCTGATCTAACCAATAGCATGAAAGCTTCTACGATGAGCGTGTCATCAAAGAAGCCACAAGCAAAACACAACGACAT
TTCAGCAACAGAAAACTCACCGGATAAGCTGGGGCTCAGTAACCCAGCACTTTGGTACAGTGGCGGCTTTATCGCACTTT
TCGTGGCCATTGCGCTTTATGATGGCGAACTGTTATCTACATTGGTAAACACAGGATTTGTATGGGCGGTGAAAGTCTTC
GGTCCATATTGGCAACTACTACTTCTTCTCACTTTTCTTATTGGTCTTGGCTTGGCTGTAGGGCGAACAGGTAAGGTGAT
TTTAGGTGGTACAACCAAGCCTGAAATGGACGGTTTTCGCTGGATGGCGATCATCTTCTGTACCTTACTCGCCGGTGGTG
GCGTGTTTTGGGCGGCAGCAGAACCAATCGCACATTATGTCAGCCCACCACCTTTGTATGGTGCACAAGATAATCCACAG
CAGGGCGCAGTAAATGCATTATCGCAGTCTTTCATGCACTGGGGTTTTCTTGCTTGGGCCATCGTTGGTAGCTTAACGTC
TATTGTGGTAATGCATCTACATTACGACAAAGGCTTACCACTCAAGCCGCGAATTCTGCTCTACCCAGTATTGGGTGAGC
GTGCACTGAAGGGCCAAATCGGCGCCTTGATTGATGCGTGCTGCATCATCGTGGTAGCGGCAGGCACCATTGGTCCAATC
GGCTTTTTAGGTCTACAGGTTAGCTACGCGTTAAACGCTTTGTTTGATATTCCTGATGGGTTCACGACACAGCTGATCAT
TATTCTCTTTGCCATCGTACTTTACACCGTCTCTGCGCTAAGTGGCCTGAACCGTGGCATGCAAATGTTGAGTCGTTACA
ACGTCATCTTGGCGATTGCCCTAATGGCTTACATCCTGCTATTCGGTCCAACAGACTTTATTTTCAATGGTTACATTCAA
GGCGTGGGCACCATGTTGATAACTTCATCCCTATGGCGAGCTACCGCGGTGATGAAGGTTGGTTGA

Upstream 100 bases:

>100_bases
GAACAAGGAATAGCCCGCCACCAATACAATAATAAGGCGGGCGTTAAAGTCACTTTTTGTAGCCGTTAAGGCAGCTGCAA
TAAACCTCTGGAGTCAGAGC

Downstream 100 bases:

>100_bases
GCTGGTGGACTGTATTCTTCTGGGGTTGGTTCCTAGGCTATGGGCCAATGATGGCCATCTTTATTGCTCGTATTTCACGT
GGCCGTAGCATTCGCCAAAT

Product: choline/carnitine/betaine transporter

Products: Proton [Cytoplasm]; choline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 341; Mature: 340

Protein sequence:

>341_residues
MSDLTNSMKASTMSVSSKKPQAKHNDISATENSPDKLGLSNPALWYSGGFIALFVAIALYDGELLSTLVNTGFVWAVKVF
GPYWQLLLLLTFLIGLGLAVGRTGKVILGGTTKPEMDGFRWMAIIFCTLLAGGGVFWAAAEPIAHYVSPPPLYGAQDNPQ
QGAVNALSQSFMHWGFLAWAIVGSLTSIVVMHLHYDKGLPLKPRILLYPVLGERALKGQIGALIDACCIIVVAAGTIGPI
GFLGLQVSYALNALFDIPDGFTTQLIIILFAIVLYTVSALSGLNRGMQMLSRYNVILAIALMAYILLFGPTDFIFNGYIQ
GVGTMLITSSLWRATAVMKVG

Sequences:

>Translated_341_residues
MSDLTNSMKASTMSVSSKKPQAKHNDISATENSPDKLGLSNPALWYSGGFIALFVAIALYDGELLSTLVNTGFVWAVKVF
GPYWQLLLLLTFLIGLGLAVGRTGKVILGGTTKPEMDGFRWMAIIFCTLLAGGGVFWAAAEPIAHYVSPPPLYGAQDNPQ
QGAVNALSQSFMHWGFLAWAIVGSLTSIVVMHLHYDKGLPLKPRILLYPVLGERALKGQIGALIDACCIIVVAAGTIGPI
GFLGLQVSYALNALFDIPDGFTTQLIIILFAIVLYTVSALSGLNRGMQMLSRYNVILAIALMAYILLFGPTDFIFNGYIQ
GVGTMLITSSLWRATAVMKVG
>Mature_340_residues
SDLTNSMKASTMSVSSKKPQAKHNDISATENSPDKLGLSNPALWYSGGFIALFVAIALYDGELLSTLVNTGFVWAVKVFG
PYWQLLLLLTFLIGLGLAVGRTGKVILGGTTKPEMDGFRWMAIIFCTLLAGGGVFWAAAEPIAHYVSPPPLYGAQDNPQQ
GAVNALSQSFMHWGFLAWAIVGSLTSIVVMHLHYDKGLPLKPRILLYPVLGERALKGQIGALIDACCIIVVAAGTIGPIG
FLGLQVSYALNALFDIPDGFTTQLIIILFAIVLYTVSALSGLNRGMQMLSRYNVILAIALMAYILLFGPTDFIFNGYIQG
VGTMLITSSLWRATAVMKVG

Specific function: High-affinity uptake of glycine betaine [H]

COG id: COG1292

COG function: function code M; Choline-glycine betaine transporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the BCCT transporter (TC 2.A.15) family [H]

Homologues:

Organism=Escherichia coli, GI1786506, Length=301, Percent_Identity=30.2325581395349, Blast_Score=136, Evalue=2e-33,
Organism=Escherichia coli, GI1788102, Length=226, Percent_Identity=30.5309734513274, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI1786224, Length=297, Percent_Identity=22.8956228956229, Blast_Score=91, Evalue=9e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000060
- InterPro:   IPR018093 [H]

Pfam domain/function: PF02028 BCCT [H]

EC number: NA

Molecular weight: Translated: 36586; Mature: 36455

Theoretical pI: Translated: 8.72; Mature: 8.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDLTNSMKASTMSVSSKKPQAKHNDISATENSPDKLGLSNPALWYSGGFIALFVAIALY
CCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCHHHHHHHHHHH
DGELLSTLVNTGFVWAVKVFGPYWQLLLLLTFLIGLGLAVGRTGKVILGGTTKPEMDGFR
CHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCHH
WMAIIFCTLLAGGGVFWAAAEPIAHYVSPPPLYGAQDNPQQGAVNALSQSFMHWGFLAWA
HHHHHHHHHHHCCCEEEHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
IVGSLTSIVVMHLHYDKGLPLKPRILLYPVLGERALKGQIGALIDACCIIVVAAGTIGPI
HHHHHHHHHHHHEECCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCH
GFLGLQVSYALNALFDIPDGFTTQLIIILFAIVLYTVSALSGLNRGMQMLSRYNVILAIA
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LMAYILLFGPTDFIFNGYIQGVGTMLITSSLWRATAVMKVG
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEECC
>Mature Secondary Structure 
SDLTNSMKASTMSVSSKKPQAKHNDISATENSPDKLGLSNPALWYSGGFIALFVAIALY
CCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCHHHHHHHHHHH
DGELLSTLVNTGFVWAVKVFGPYWQLLLLLTFLIGLGLAVGRTGKVILGGTTKPEMDGFR
CHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCHH
WMAIIFCTLLAGGGVFWAAAEPIAHYVSPPPLYGAQDNPQQGAVNALSQSFMHWGFLAWA
HHHHHHHHHHHCCCEEEHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
IVGSLTSIVVMHLHYDKGLPLKPRILLYPVLGERALKGQIGALIDACCIIVVAAGTIGPI
HHHHHHHHHHHHEECCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCH
GFLGLQVSYALNALFDIPDGFTTQLIIILFAIVLYTVSALSGLNRGMQMLSRYNVILAIA
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LMAYILLFGPTDFIFNGYIQGVGTMLITSSLWRATAVMKVG
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; choline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + choline [Periplasm] = Proton [Cytoplasm] + choline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8752321; 9387221; 9384377 [H]