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The map label for this gene is phbC [H]

Identifier: 156976594

GI number: 156976594

Start: 597370

End: 599145

Strand: Direct

Name: phbC [H]

Synonym: VIBHAR_05368

Alternate gene names: 156976594

Gene position: 597370-599145 (Clockwise)

Preceding gene: 156976593

Following gene: 156976596

Centisome position: 27.1

GC content: 45.66

Gene sequence:

>1776_bases
ATGTTTCAACACTTCTTTTCGGACTATCTTGTAAAGCTTCAGGAAACCAATCATCAGTGGTGGCATGATTTCGAAGTGAA
CAAGGCAGTCGTGAATTCTCCTTTGAATAAGGCTATGCAAGAAGTGAACTTCGAGGATACTGCTAAACTGTTCGAACAAG
CGGCGAACCAACCTGCTGCCATCTTGAAATTGCAAGCAGAGTGGTGGGAACAGCAGCTTCAAATTTGGCAGAATGTCGCT
CTCGCAGGCAATCAAACACAGATTATTGAAGCAGAGAAGGGTGATAAGCGTTTCTCAAACGATGCTTGGCAGCAAGAAGC
CATGTACAGCTTTATCAAACAATCTTATTTGTTATTTAGCAAAACCTACCTAGACACACTCGACTCAATCGAAGGGTTGG
ATGAGAAAACCAAAGAGCGTATTACCTTCTTCTCTCGTCAAGCCATCAACGCACTTTCCCCATCAAACTTCATTGCGACT
AACCCTGAGCTGCTAAAACTCACTCTTGAGCAAAATGGTGAGAACCTCCTAACGGGTTTAGAGAAGTTAAAAGAAGATGT
CGAGTCGAGTGCGGATATTCTCAAAGTGCGCATGACCAACAACAATGCGTTTCGTGTCGGTGATGACGTTGCAACGACAG
CGGGCGATGTGGTTTTCCAAAACGAACTATTTGAGTTGATTCAGTATCGTCCGTTGACAGAGAAAGTGAATGCGACACCG
TTGCTGATCGTTCCACCTTTTATTAATAAATACTACATCCTTGACCTAACGGCGAAGAACTCCATGGTTCGTTGGCTATT
AGAGCAAGGCCATAGCGTATTTATGATGTCGTGGCAAAACCCAGGTAAAGAGCAGGCTCACGTTGAGTTTGGTGACTACG
TGACGGAAGGCGTCGCTAAAGCTGTGACTGCAATTGAAGACATCACAGGTCAAGAGCAAATCAACGCAGCGGGTTATTGC
ATTGGTGGTACGGTTCTGGCGAGCACGGTGGCGTACTATGCTGCGAAGCGTATGAAGAAGCGCATTAAGTCTGCAAGCTT
CTTTACCACGCTACTGGACTTCTCTCAGCCGGGCGAAGTAGGGGCGTACATCAACGACACGATTATTAGTGCCATTGAAA
CGCAGAATAATGCGAAAGGTTACATGGACGGTCGTTCGTTAAGTGTGACATTTAGCTTACTCCGCGAAAACAGTCTGTAC
TGGAACTACTATGTCGATAATTACTTGAAAGGTAATAGCCCGGTTGATTTTGATCTGCTTTACTGGAACAGCGACAGTAC
GAACGTGAGTGCCGCTTCTCATAACTTCTTATTGCGTGAACTCTATCTAGAGAACAAGCTAGTCCAAGACAAGGGCGTGA
AGATCGGTGGCGTGTGGATCGACCTGAATAAGATTAAGATCCCAAGCTATTTTGTGTCGACCAAAGAAGACCATATCGCG
TTGTGGCAGGGTACATATCGCGGCGCACTAAACACAGGTGGCAACAAGACCTTTGTTTTGGGTGAATCAGGGCACATTGC
TGGTATCGTCAATCACCCTGCAAAAAACAAATACGGCTATTGGCTAAACGACAACCTTGATGACTCGGCAGATGAGTGGT
TTAACAACGCGAATCATCAAGAAGGCTCTTGGTGGACGCATTGGCATCAATGGCTGTTGCAATTCAACCCAGAAGAGCAA
GTTGAACCATTCCCAGTGGGTTCTGAGCAGAACCCTGTGATTGATGAAGCGCCTGGTCAATACGTAAAGCAAGTTTTGCC
TATTAAAGAGTCCTAA

Upstream 100 bases:

>100_bases
TTCGGAAAACCTAAAGACTGTTACTCCTGCGTAATATGTCCACTTATCGCCGCCCGCATTCTTGGGCGGCGTTGTTTTTT
CCGAATATAGGAGTAAGACT

Downstream 100 bases:

>100_bases
ACTGCTCCTCACAGCAGTCTCATTTGGCGGCCTACTATAATGGTCGCCTTTTTTGTTTCTAACGTTTCAGGTTAAAGCGG
GGAAGGATACAGTAATTGTC

Product: hypothetical protein

Products: NA

Alternate protein names: PHA synthase; PHB synthase; Poly(3-hydroxyalkanoate) polymerase; PHA polymerase; Poly(3-hydroxybutyrate) polymerase; PHB polymerase; Polyhydroxyalkanoic acid synthase [H]

Number of amino acids: Translated: 591; Mature: 591

Protein sequence:

>591_residues
MFQHFFSDYLVKLQETNHQWWHDFEVNKAVVNSPLNKAMQEVNFEDTAKLFEQAANQPAAILKLQAEWWEQQLQIWQNVA
LAGNQTQIIEAEKGDKRFSNDAWQQEAMYSFIKQSYLLFSKTYLDTLDSIEGLDEKTKERITFFSRQAINALSPSNFIAT
NPELLKLTLEQNGENLLTGLEKLKEDVESSADILKVRMTNNNAFRVGDDVATTAGDVVFQNELFELIQYRPLTEKVNATP
LLIVPPFINKYYILDLTAKNSMVRWLLEQGHSVFMMSWQNPGKEQAHVEFGDYVTEGVAKAVTAIEDITGQEQINAAGYC
IGGTVLASTVAYYAAKRMKKRIKSASFFTTLLDFSQPGEVGAYINDTIISAIETQNNAKGYMDGRSLSVTFSLLRENSLY
WNYYVDNYLKGNSPVDFDLLYWNSDSTNVSAASHNFLLRELYLENKLVQDKGVKIGGVWIDLNKIKIPSYFVSTKEDHIA
LWQGTYRGALNTGGNKTFVLGESGHIAGIVNHPAKNKYGYWLNDNLDDSADEWFNNANHQEGSWWTHWHQWLLQFNPEEQ
VEPFPVGSEQNPVIDEAPGQYVKQVLPIKES

Sequences:

>Translated_591_residues
MFQHFFSDYLVKLQETNHQWWHDFEVNKAVVNSPLNKAMQEVNFEDTAKLFEQAANQPAAILKLQAEWWEQQLQIWQNVA
LAGNQTQIIEAEKGDKRFSNDAWQQEAMYSFIKQSYLLFSKTYLDTLDSIEGLDEKTKERITFFSRQAINALSPSNFIAT
NPELLKLTLEQNGENLLTGLEKLKEDVESSADILKVRMTNNNAFRVGDDVATTAGDVVFQNELFELIQYRPLTEKVNATP
LLIVPPFINKYYILDLTAKNSMVRWLLEQGHSVFMMSWQNPGKEQAHVEFGDYVTEGVAKAVTAIEDITGQEQINAAGYC
IGGTVLASTVAYYAAKRMKKRIKSASFFTTLLDFSQPGEVGAYINDTIISAIETQNNAKGYMDGRSLSVTFSLLRENSLY
WNYYVDNYLKGNSPVDFDLLYWNSDSTNVSAASHNFLLRELYLENKLVQDKGVKIGGVWIDLNKIKIPSYFVSTKEDHIA
LWQGTYRGALNTGGNKTFVLGESGHIAGIVNHPAKNKYGYWLNDNLDDSADEWFNNANHQEGSWWTHWHQWLLQFNPEEQ
VEPFPVGSEQNPVIDEAPGQYVKQVLPIKES
>Mature_591_residues
MFQHFFSDYLVKLQETNHQWWHDFEVNKAVVNSPLNKAMQEVNFEDTAKLFEQAANQPAAILKLQAEWWEQQLQIWQNVA
LAGNQTQIIEAEKGDKRFSNDAWQQEAMYSFIKQSYLLFSKTYLDTLDSIEGLDEKTKERITFFSRQAINALSPSNFIAT
NPELLKLTLEQNGENLLTGLEKLKEDVESSADILKVRMTNNNAFRVGDDVATTAGDVVFQNELFELIQYRPLTEKVNATP
LLIVPPFINKYYILDLTAKNSMVRWLLEQGHSVFMMSWQNPGKEQAHVEFGDYVTEGVAKAVTAIEDITGQEQINAAGYC
IGGTVLASTVAYYAAKRMKKRIKSASFFTTLLDFSQPGEVGAYINDTIISAIETQNNAKGYMDGRSLSVTFSLLRENSLY
WNYYVDNYLKGNSPVDFDLLYWNSDSTNVSAASHNFLLRELYLENKLVQDKGVKIGGVWIDLNKIKIPSYFVSTKEDHIA
LWQGTYRGALNTGGNKTFVLGESGHIAGIVNHPAKNKYGYWLNDNLDDSADEWFNNANHQEGSWWTHWHQWLLQFNPEEQ
VEPFPVGSEQNPVIDEAPGQYVKQVLPIKES

Specific function: Polymerizes d(-)-3-hydroxybutyryl-CoA to create PHB which consists of thousands of hydroxybutyrate molecules linked end to end. PHB serves as an intracellular energy reserve material when cells grow under conditions of nutrient limitation [H]

COG id: COG3243

COG function: function code I; Poly(3-hydroxyalkanoate) synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PHA/PHB synthase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR010963
- InterPro:   IPR010941 [H]

Pfam domain/function: PF00561 Abhydrolase_1; PF07167 PhaC_N [H]

EC number: NA

Molecular weight: Translated: 67317; Mature: 67317

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFQHFFSDYLVKLQETNHQWWHDFEVNKAVVNSPLNKAMQEVNFEDTAKLFEQAANQPAA
CCHHHHHHHHHHHHCCCCCCCEECCCCHHHHCCHHHHHHHHCCHHHHHHHHHHHCCCCCE
ILKLQAEWWEQQLQIWQNVALAGNQTQIIEAEKGDKRFSNDAWQQEAMYSFIKQSYLLFS
EEEEEHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
KTYLDTLDSIEGLDEKTKERITFFSRQAINALSPSNFIATNPELLKLTLEQNGENLLTGL
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCEEECCHHEEEEEECCCCCHHHHHH
EKLKEDVESSADILKVRMTNNNAFRVGDDVATTAGDVVFQNELFELIQYRPLTEKVNATP
HHHHHHHHCCCEEEEEEEECCCCEEECCCHHHCCCCCEEHHHHHHHHHCCCCHHHCCCCE
LLIVPPFINKYYILDLTAKNSMVRWLLEQGHSVFMMSWQNPGKEQAHVEFGDYVTEGVAK
EEEECCCCCCEEEEEEECCHHHHHHHHHCCCEEEEEECCCCCCCCCEEEHHHHHHHHHHH
AVTAIEDITGQEQINAAGYCIGGTVLASTVAYYAAKRMKKRIKSASFFTTLLDFSQPGEV
HHHHHHHCCCCHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
GAYINDTIISAIETQNNAKGYMDGRSLSVTFSLLRENSLYWNYYVDNYLKGNSPVDFDLL
CCCHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEECCCCEEEEEEECCEECCCCCCEEEEE
YWNSDSTNVSAASHNFLLRELYLENKLVQDKGVKIGGVWIDLNKIKIPSYFVSTKEDHIA
EECCCCCCEEHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECEEECCEEEECCCCCCEE
LWQGTYRGALNTGGNKTFVLGESGHIAGIVNHPAKNKYGYWLNDNLDDSADEWFNNANHQ
EEECCCCCCCCCCCCEEEEEECCCCEEEEECCCCCCCCCEEECCCCCCCHHHHHCCCCCC
EGSWWTHWHQWLLQFNPEEQVEPFPVGSEQNPVIDEAPGQYVKQVLPIKES
CCCHHHHHHHHHHCCCCHHCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCC
>Mature Secondary Structure
MFQHFFSDYLVKLQETNHQWWHDFEVNKAVVNSPLNKAMQEVNFEDTAKLFEQAANQPAA
CCHHHHHHHHHHHHCCCCCCCEECCCCHHHHCCHHHHHHHHCCHHHHHHHHHHHCCCCCE
ILKLQAEWWEQQLQIWQNVALAGNQTQIIEAEKGDKRFSNDAWQQEAMYSFIKQSYLLFS
EEEEEHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
KTYLDTLDSIEGLDEKTKERITFFSRQAINALSPSNFIATNPELLKLTLEQNGENLLTGL
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCEEECCHHEEEEEECCCCCHHHHHH
EKLKEDVESSADILKVRMTNNNAFRVGDDVATTAGDVVFQNELFELIQYRPLTEKVNATP
HHHHHHHHCCCEEEEEEEECCCCEEECCCHHHCCCCCEEHHHHHHHHHCCCCHHHCCCCE
LLIVPPFINKYYILDLTAKNSMVRWLLEQGHSVFMMSWQNPGKEQAHVEFGDYVTEGVAK
EEEECCCCCCEEEEEEECCHHHHHHHHHCCCEEEEEECCCCCCCCCEEEHHHHHHHHHHH
AVTAIEDITGQEQINAAGYCIGGTVLASTVAYYAAKRMKKRIKSASFFTTLLDFSQPGEV
HHHHHHHCCCCHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
GAYINDTIISAIETQNNAKGYMDGRSLSVTFSLLRENSLYWNYYVDNYLKGNSPVDFDLL
CCCHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEECCCCEEEEEEECCEECCCCCCEEEEE
YWNSDSTNVSAASHNFLLRELYLENKLVQDKGVKIGGVWIDLNKIKIPSYFVSTKEDHIA
EECCCCCCEEHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECEEECCEEEECCCCCCEE
LWQGTYRGALNTGGNKTFVLGESGHIAGIVNHPAKNKYGYWLNDNLDDSADEWFNNANHQ
EEECCCCCCCCCCCCEEEEEECCCCEEEEECCCCCCCCCEEECCCCCCCHHHHHCCCCCC
EGSWWTHWHQWLLQFNPEEQVEPFPVGSEQNPVIDEAPGQYVKQVLPIKES
CCCHHHHHHHHHHCCCCHHCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9748438 [H]