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The map label for this gene is eptA [H]

Identifier: 156976516

GI number: 156976516

Start: 527896

End: 529548

Strand: Reverse

Name: eptA [H]

Synonym: VIBHAR_05289

Alternate gene names: 156976516

Gene position: 529548-527896 (Counterclockwise)

Preceding gene: 156976517

Following gene: 156976515

Centisome position: 24.03

GC content: 43.98

Gene sequence:

>1653_bases
ATGAAGACAATTGAACTCCCAACTAAAGGTATCTCCTACATTACCTTTACGCTTTTATTAGCCTTGTATTTCGCTCTTGT
AGTCAATATCCCCATCTACAAAGAGTTGGTGCATATCTTTGCGGATCTCGACCAGGTTAAGATCGGTTTCATTATTACGA
TTCCAATCTTCTTTTTTGCGGCGTTGAATTTCCTATTTAACCTGTTCAGTTGGCCTTGGATCAGCAAGCCTTTCTTCATC
TTATTGCTGATCACATCGGGCATGGTGAGTTACGCAAGCTACAACTACGGCACCTTGTTCGATACGGACATGATCACCAA
CATCGTTGAGACAGATACCAGTGAAGCCAGCTCATACTTGAGTGCCTACTCACTTATCTGGACACTGATTATGGGAGTTG
TTCCCGCTATCTTGGTCTTCAAGGTGAAACTCAAGCCCATAGAAGGTAAATGGCTTCGATTTACATTAACTAAAGCCGTA
TCTATGCTGGCTTCATTGGCGATAATCGCCGTGATTGCAGGTTTGTACTACCAAGACTATGCGTCTGTCGGCCGAAACAA
TAGTTACCTAAAGAAAATCATCATCCCTACTCAGTATGTGTATGCCATTTCTAGCTACGTAAAAGAAAATTACTTAACGA
CGCCAGAGCCTTACCGCGAAATTGGTACGGATGCAAAACAGTCCCAACTAGCACTGGAACAAGCAAAAGAAAAACCGACG
CTGCTTGTCTTTGTGCTAGGTGAAACGGCACGTACTCAAAACTACCAAATCAACGGTTATGATCGTGAAACCAACCCGTA
CACCAGCAAGTTAGATGTGATTTCATTCCAAGACGTCTCTTCTTGCGGGACGGCGACCGCGGTTTCTGTGCCTTGTATGT
TCTCTCAATTACCTCGCAGTGATTTCGACCGTAGCAAAGCAGATAACCAAGACAATGCCTTAGATATCATGCAACGTGCT
GGCATCGATCTCATGTGGAAAGAAAATGATGGTGGTGACAAAGGCGTTGCCCATAAGATCAACAAAATTGAAGTCGATCG
CAGACAAAAAAATGAATTTTGCAACGGCAGCACCTGCTATGACATTGCATTGCTTGAAAACATCGAAGAACAAATTGGTG
GCATGAAAGGTAACCGCCTTGTTGCATTTCACCTCATTGGCAGTCATGGGCCGACGTATTTCCAACGTTACCCGAAAGAC
AAAGCTTTCTTCCAACCAGACTGTCCTCGTGCAGACATTGAAAACTGCAGCGTTGAGCAAATCGTTAACTCCTACGACAA
CACGATTCGCTACACAGACTTCGTCTTGGATCAAACCATTGCGAAATTGAAAACGTTAGAAGACAAATACAATACCGCGA
TGATCTACGTCTCTGACCACGGCGAGTCTTTGGGCGAAAATGGCTTGTTCTTACATGGCATGCCATATGGGTTAGCACCA
GATTTTCAAAAACGCGTACCCATGATGGTTTGGATGTCTCCTGGCTTTAAACAAGCCAAACACATCAATGCCGATTGCTT
GGCACAAGAAGCCCAGCAAACAGCAACGCATTCACACGATAATGTATTCCACTCGTTGTTGGGGATCATGGATGTTGAAA
CCAATGCCTATGACGGTAAGTTGGATATCTTTAAAACGTGCAGAACCTCTTAA

Upstream 100 bases:

>100_bases
AGCCAAAGACATTGGCTCCGCAGCAGTATTTGTTGCACTTTTTCTCGCCCTATTTGTTTGGGCGTCGATTTTACTTTAGT
CACATCGAAGCACGTCAATT

Downstream 100 bases:

>100_bases
CGAGCTCTTGTAGGAGTTCGTAACAAGACAATATGAAAGTAAAGCCTGACCACGACGTGAGTCAGGCTTTTTTATGACAC
CTCGCTCCAAGAAATGCCAG

Product: lipid A phosphoethanolamine transferase

Products: NA

Alternate protein names: Polymyxin resistance protein pmrC [H]

Number of amino acids: Translated: 550; Mature: 550

Protein sequence:

>550_residues
MKTIELPTKGISYITFTLLLALYFALVVNIPIYKELVHIFADLDQVKIGFIITIPIFFFAALNFLFNLFSWPWISKPFFI
LLLITSGMVSYASYNYGTLFDTDMITNIVETDTSEASSYLSAYSLIWTLIMGVVPAILVFKVKLKPIEGKWLRFTLTKAV
SMLASLAIIAVIAGLYYQDYASVGRNNSYLKKIIIPTQYVYAISSYVKENYLTTPEPYREIGTDAKQSQLALEQAKEKPT
LLVFVLGETARTQNYQINGYDRETNPYTSKLDVISFQDVSSCGTATAVSVPCMFSQLPRSDFDRSKADNQDNALDIMQRA
GIDLMWKENDGGDKGVAHKINKIEVDRRQKNEFCNGSTCYDIALLENIEEQIGGMKGNRLVAFHLIGSHGPTYFQRYPKD
KAFFQPDCPRADIENCSVEQIVNSYDNTIRYTDFVLDQTIAKLKTLEDKYNTAMIYVSDHGESLGENGLFLHGMPYGLAP
DFQKRVPMMVWMSPGFKQAKHINADCLAQEAQQTATHSHDNVFHSLLGIMDVETNAYDGKLDIFKTCRTS

Sequences:

>Translated_550_residues
MKTIELPTKGISYITFTLLLALYFALVVNIPIYKELVHIFADLDQVKIGFIITIPIFFFAALNFLFNLFSWPWISKPFFI
LLLITSGMVSYASYNYGTLFDTDMITNIVETDTSEASSYLSAYSLIWTLIMGVVPAILVFKVKLKPIEGKWLRFTLTKAV
SMLASLAIIAVIAGLYYQDYASVGRNNSYLKKIIIPTQYVYAISSYVKENYLTTPEPYREIGTDAKQSQLALEQAKEKPT
LLVFVLGETARTQNYQINGYDRETNPYTSKLDVISFQDVSSCGTATAVSVPCMFSQLPRSDFDRSKADNQDNALDIMQRA
GIDLMWKENDGGDKGVAHKINKIEVDRRQKNEFCNGSTCYDIALLENIEEQIGGMKGNRLVAFHLIGSHGPTYFQRYPKD
KAFFQPDCPRADIENCSVEQIVNSYDNTIRYTDFVLDQTIAKLKTLEDKYNTAMIYVSDHGESLGENGLFLHGMPYGLAP
DFQKRVPMMVWMSPGFKQAKHINADCLAQEAQQTATHSHDNVFHSLLGIMDVETNAYDGKLDIFKTCRTS
>Mature_550_residues
MKTIELPTKGISYITFTLLLALYFALVVNIPIYKELVHIFADLDQVKIGFIITIPIFFFAALNFLFNLFSWPWISKPFFI
LLLITSGMVSYASYNYGTLFDTDMITNIVETDTSEASSYLSAYSLIWTLIMGVVPAILVFKVKLKPIEGKWLRFTLTKAV
SMLASLAIIAVIAGLYYQDYASVGRNNSYLKKIIIPTQYVYAISSYVKENYLTTPEPYREIGTDAKQSQLALEQAKEKPT
LLVFVLGETARTQNYQINGYDRETNPYTSKLDVISFQDVSSCGTATAVSVPCMFSQLPRSDFDRSKADNQDNALDIMQRA
GIDLMWKENDGGDKGVAHKINKIEVDRRQKNEFCNGSTCYDIALLENIEEQIGGMKGNRLVAFHLIGSHGPTYFQRYPKD
KAFFQPDCPRADIENCSVEQIVNSYDNTIRYTDFVLDQTIAKLKTLEDKYNTAMIYVSDHGESLGENGLFLHGMPYGLAP
DFQKRVPMMVWMSPGFKQAKHINADCLAQEAQQTATHSHDNVFHSLLGIMDVETNAYDGKLDIFKTCRTS

Specific function: Catalyzes the addition of a phosphoethanolamine moiety to the lipid A. The phosphoethanolamine modification is required for resistance to polymyxin [H]

COG id: COG2194

COG function: function code R; Predicted membrane-associated, metal-dependent hydrolase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoethanolamine transferase family. EptA subfamily [H]

Homologues:

Organism=Escherichia coli, GI87082372, Length=527, Percent_Identity=38.5199240986717, Blast_Score=410, Evalue=1e-115,
Organism=Escherichia coli, GI87082286, Length=477, Percent_Identity=26.2054507337526, Blast_Score=146, Evalue=3e-36,
Organism=Escherichia coli, GI87082223, Length=448, Percent_Identity=22.7678571428571, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1790392, Length=390, Percent_Identity=25.3846153846154, Blast_Score=76, Evalue=6e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017849
- InterPro:   IPR017850
- InterPro:   IPR012549
- InterPro:   IPR000917 [H]

Pfam domain/function: PF08019 DUF1705; PF00884 Sulfatase [H]

EC number: NA

Molecular weight: Translated: 62118; Mature: 62118

Theoretical pI: Translated: 5.84; Mature: 5.84

Prosite motif: PS00599 AA_TRANSFER_CLASS_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTIELPTKGISYITFTLLLALYFALVVNIPIYKELVHIFADLDQVKIGFIITIPIFFFA
CCEECCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCEEEHHHHHHHHHHHH
ALNFLFNLFSWPWISKPFFILLLITSGMVSYASYNYGTLFDTDMITNIVETDTSEASSYL
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEECHHHHHHHHHCCCHHHHHHH
SAYSLIWTLIMGVVPAILVFKVKLKPIEGKWLRFTLTKAVSMLASLAIIAVIAGLYYQDY
HHHHHHHHHHHHHHHHHHHEEEEEECCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHH
ASVGRNNSYLKKIIIPTQYVYAISSYVKENYLTTPEPYREIGTDAKQSQLALEQAKEKPT
HHHCCCCHHHHHEECCHHHHHHHHHHHHHCCCCCCCHHHHHCCCCHHHHHHHHHHCCCCC
LLVFVLGETARTQNYQINGYDRETNPYTSKLDVISFQDVSSCGTATAVSVPCMFSQLPRS
EEEEEECCCCCCCCEEECCCCCCCCCCCCCEEEEEECCHHCCCCCHHEEHHHHHHHCCCC
DFDRSKADNQDNALDIMQRAGIDLMWKENDGGDKGVAHKINKIEVDRRQKNEFCNGSTCY
CCCCCCCCCCCHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHEECCHHHHCCCCCCCCHH
DIALLENIEEQIGGMKGNRLVAFHLIGSHGPTYFQRYPKDKAFFQPDCPRADIENCSVEQ
HHHHHHHHHHHHCCCCCCEEEEEEEECCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCHHH
IVNSYDNTIRYTDFVLDQTIAKLKTLEDKYNTAMIYVSDHGESLGENGLFLHGMPYGLAP
HHHHHCCCEEHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCEEEECCCCCCCC
DFQKRVPMMVWMSPGFKQAKHINADCLAQEAQQTATHSHDNVFHSLLGIMDVETNAYDGK
CHHHCCCEEEEECCCCHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCC
LDIFKTCRTS
HHHHHHHCCC
>Mature Secondary Structure
MKTIELPTKGISYITFTLLLALYFALVVNIPIYKELVHIFADLDQVKIGFIITIPIFFFA
CCEECCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCEEEHHHHHHHHHHHH
ALNFLFNLFSWPWISKPFFILLLITSGMVSYASYNYGTLFDTDMITNIVETDTSEASSYL
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEECHHHHHHHHHCCCHHHHHHH
SAYSLIWTLIMGVVPAILVFKVKLKPIEGKWLRFTLTKAVSMLASLAIIAVIAGLYYQDY
HHHHHHHHHHHHHHHHHHHEEEEEECCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHH
ASVGRNNSYLKKIIIPTQYVYAISSYVKENYLTTPEPYREIGTDAKQSQLALEQAKEKPT
HHHCCCCHHHHHEECCHHHHHHHHHHHHHCCCCCCCHHHHHCCCCHHHHHHHHHHCCCCC
LLVFVLGETARTQNYQINGYDRETNPYTSKLDVISFQDVSSCGTATAVSVPCMFSQLPRS
EEEEEECCCCCCCCEEECCCCCCCCCCCCCEEEEEECCHHCCCCCHHEEHHHHHHHCCCC
DFDRSKADNQDNALDIMQRAGIDLMWKENDGGDKGVAHKINKIEVDRRQKNEFCNGSTCY
CCCCCCCCCCCHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHEECCHHHHCCCCCCCCHH
DIALLENIEEQIGGMKGNRLVAFHLIGSHGPTYFQRYPKDKAFFQPDCPRADIENCSVEQ
HHHHHHHHHHHHCCCCCCEEEEEEEECCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCHHH
IVNSYDNTIRYTDFVLDQTIAKLKTLEDKYNTAMIYVSDHGESLGENGLFLHGMPYGLAP
HHHHHCCCEEHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCEEEECCCCCCCC
DFQKRVPMMVWMSPGFKQAKHINADCLAQEAQQTATHSHDNVFHSLLGIMDVETNAYDGK
CHHHCCCEEEEECCCCHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCC
LDIFKTCRTS
HHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8391535; 11677609 [H]