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The map label for this gene is hemN [H]

Identifier: 156976472

GI number: 156976472

Start: 495849

End: 497219

Strand: Direct

Name: hemN [H]

Synonym: VIBHAR_05245

Alternate gene names: 156976472

Gene position: 495849-497219 (Clockwise)

Preceding gene: 156976465

Following gene: 156976473

Centisome position: 22.5

GC content: 47.34

Gene sequence:

>1371_bases
ATGACCGTCGATATTTATAAATTTGACGAATCAATCCTTGGGAGTGATAGCCCCGATCCGTTGCGCTTTGCTTTTGTGAA
AAAACACTCTGCGCATGCGGGTGGCAGTAGCATGCCAGTGCCGCCGCCACAACAAGGTGACATCCTATCGAGAATCACCT
CGGAAACGACGACTAAGAACAATAAGCGCTGCTTATACATTCATGTTCCATTCTGTCGAGTGCGCTGTACTTTCTGTAAC
TTCTTCCAAAATGCTGCCAGCCGTAAGTTAGTCGATGAATACTTTGATGCTCTGATGAAAGAGCTAAAAGAGAAAGCGGC
GATGCCGTGGACGCAAAGTGGCATCTTCCATGCGGTTTACATCGGCGGTGGCACGCCAACGGATCTGGCACCAGAACAAG
TTCGCATTCTTGGTCAGGCCATTCACGATTATTTCCCTCTAGCAGCTGACTGCGAAATTACACTTGAAGGTCGTATCAAC
CGATTCTCTGACGAGTTATATCAAGGTGCGCTAGACGGCGGATTCAACCGTTTCTCATTTGGTGTGCAGAGCTTTAATAC
CAAAGTTCGTCGCAGCGCTAAGCGTTTGGATGATCGCGAAGACGTATTGAATCGCGTTAGTGAGTTGGCAAAAGAAGACA
AGATCCCTGTCATCATTGATTTACTTTACGGCCTGCCTTACCAAACAAAAGACGTTTTAGAGCAAGATCTTAAGGACTTC
ATGTCTACAGGCGCTCATGGTTTGGACCTTTATCAGTTAGTGGTTGGCGGTACAGCGCCAATGCTCAATTTGGTTGAAAA
GGGCAAAATGTCGCCACCTGCAACGACACCGGAAAAAGCAGGCATGTACGAGCTTGGTGCTAAGTTTATGGCAGAGCATC
ACATGAAGCAGCTTAGCGTGAACCACTGGGCGATCGATAACCGTGAACGCAGTTTATACAACAGCTTAGCGAAAACCTAT
GCAGAAGTTCTGCCTGTCGGTTGTGGCGCAGGCGGCAACATCGGTGGTTACGGCATGATGCAGCACCGCACGCTAGACAC
TTACGTTAAAGCTGTAAATGAAGGGCAGAGCACCATCGCAATGATGATGAAGCAAAGCTCACTAGAGCCACTATTCGCAG
CGCTGAAATCCGGCTTTGATCGAGGCATTGTTCAGGCGAGCAAGCTGCCAGCATTTATGGGCGAAGACACCTTTAATTTC
CTGATGCCACTATTCAAAGTTTGGCATGACAAAGGTTTGGTCGAGTTACAAGAGCGCAGTTTAGTTCTGACGTTAGCAGG
TAGCTTCTGGGCTGTGTCTTTGGCTCAAGCATGTATTCAAGTACTGACTTCTGAAATGAACGAAAACGTACCAAAAGTAT
CTAACAATTAA

Upstream 100 bases:

>100_bases
CAATTATCAATTGCATTATCATTACGGCCAAATTATGATCTTTGCAGTTTTTTTGAAATGAGCCTCAATCCTTTGTGTGG
TGGGCTTTAAGTGAGTAAAT

Downstream 100 bases:

>100_bases
TCTGAAAGAAATGGAAAAAACAATGGAATCAATTAAACAACAGGTCGAAGCCCTACTAGAACAAGAGCCGCAACTACTTC
CTGCACCAATGGCTGAGCGC

Product: coproporphyrinogen III oxidase

Products: NA

Alternate protein names: Coprogen oxidase; Coproporphyrinogenase [H]

Number of amino acids: Translated: 456; Mature: 455

Protein sequence:

>456_residues
MTVDIYKFDESILGSDSPDPLRFAFVKKHSAHAGGSSMPVPPPQQGDILSRITSETTTKNNKRCLYIHVPFCRVRCTFCN
FFQNAASRKLVDEYFDALMKELKEKAAMPWTQSGIFHAVYIGGGTPTDLAPEQVRILGQAIHDYFPLAADCEITLEGRIN
RFSDELYQGALDGGFNRFSFGVQSFNTKVRRSAKRLDDREDVLNRVSELAKEDKIPVIIDLLYGLPYQTKDVLEQDLKDF
MSTGAHGLDLYQLVVGGTAPMLNLVEKGKMSPPATTPEKAGMYELGAKFMAEHHMKQLSVNHWAIDNRERSLYNSLAKTY
AEVLPVGCGAGGNIGGYGMMQHRTLDTYVKAVNEGQSTIAMMMKQSSLEPLFAALKSGFDRGIVQASKLPAFMGEDTFNF
LMPLFKVWHDKGLVELQERSLVLTLAGSFWAVSLAQACIQVLTSEMNENVPKVSNN

Sequences:

>Translated_456_residues
MTVDIYKFDESILGSDSPDPLRFAFVKKHSAHAGGSSMPVPPPQQGDILSRITSETTTKNNKRCLYIHVPFCRVRCTFCN
FFQNAASRKLVDEYFDALMKELKEKAAMPWTQSGIFHAVYIGGGTPTDLAPEQVRILGQAIHDYFPLAADCEITLEGRIN
RFSDELYQGALDGGFNRFSFGVQSFNTKVRRSAKRLDDREDVLNRVSELAKEDKIPVIIDLLYGLPYQTKDVLEQDLKDF
MSTGAHGLDLYQLVVGGTAPMLNLVEKGKMSPPATTPEKAGMYELGAKFMAEHHMKQLSVNHWAIDNRERSLYNSLAKTY
AEVLPVGCGAGGNIGGYGMMQHRTLDTYVKAVNEGQSTIAMMMKQSSLEPLFAALKSGFDRGIVQASKLPAFMGEDTFNF
LMPLFKVWHDKGLVELQERSLVLTLAGSFWAVSLAQACIQVLTSEMNENVPKVSNN
>Mature_455_residues
TVDIYKFDESILGSDSPDPLRFAFVKKHSAHAGGSSMPVPPPQQGDILSRITSETTTKNNKRCLYIHVPFCRVRCTFCNF
FQNAASRKLVDEYFDALMKELKEKAAMPWTQSGIFHAVYIGGGTPTDLAPEQVRILGQAIHDYFPLAADCEITLEGRINR
FSDELYQGALDGGFNRFSFGVQSFNTKVRRSAKRLDDREDVLNRVSELAKEDKIPVIIDLLYGLPYQTKDVLEQDLKDFM
STGAHGLDLYQLVVGGTAPMLNLVEKGKMSPPATTPEKAGMYELGAKFMAEHHMKQLSVNHWAIDNRERSLYNSLAKTYA
EVLPVGCGAGGNIGGYGMMQHRTLDTYVKAVNEGQSTIAMMMKQSSLEPLFAALKSGFDRGIVQASKLPAFMGEDTFNFL
MPLFKVWHDKGLVELQERSLVLTLAGSFWAVSLAQACIQVLTSEMNENVPKVSNN

Specific function: Anaerobic transformation of coproporphyrinogen-III into protoporphyrinogen-IX [H]

COG id: COG0635

COG function: function code H; Coproporphyrinogen III oxidase and related Fe-S oxidoreductases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the anaerobic coproporphyrinogen-III oxidase family [H]

Homologues:

Organism=Homo sapiens, GI8922911, Length=314, Percent_Identity=28.6624203821656, Blast_Score=98, Evalue=2e-20,
Organism=Escherichia coli, GI87082341, Length=380, Percent_Identity=23.9473684210526, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI1789325, Length=290, Percent_Identity=27.9310344827586, Blast_Score=96, Evalue=7e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR004559
- InterPro:   IPR010723
- InterPro:   IPR007197 [H]

Pfam domain/function: PF06969 HemN_C; PF04055 Radical_SAM [H]

EC number: =1.3.99.22 [H]

Molecular weight: Translated: 50683; Mature: 50551

Theoretical pI: Translated: 6.74; Mature: 6.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVDIYKFDESILGSDSPDPLRFAFVKKHSAHAGGSSMPVPPPQQGDILSRITSETTTKN
CEEEEEECCHHHCCCCCCCCCEEHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCC
NKRCLYIHVPFCRVRCTFCNFFQNAASRKLVDEYFDALMKELKEKAAMPWTQSGIFHAVY
CCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEE
IGGGTPTDLAPEQVRILGQAIHDYFPLAADCEITLEGRINRFSDELYQGALDGGFNRFSF
ECCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEECCCHHHHHHHHHHHHHCCCCHHHHH
GVQSFNTKVRRSAKRLDDREDVLNRVSELAKEDKIPVIIDLLYGLPYQTKDVLEQDLKDF
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCHHHHHHHHHHHH
MSTGAHGLDLYQLVVGGTAPMLNLVEKGKMSPPATTPEKAGMYELGAKFMAEHHMKQLSV
HHCCCCCHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCC
NHWAIDNRERSLYNSLAKTYAEVLPVGCGAGGNIGGYGMMQHRTLDTYVKAVNEGQSTIA
CHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHH
MMMKQSSLEPLFAALKSGFDRGIVQASKLPAFMGEDTFNFLMPLFKVWHDKGLVELQERS
HHHHHHCCHHHHHHHHCCHHHHHHHHHHCCHHHCCCHHHHHHHHHHHHHCCCCCEECCCC
LVLTLAGSFWAVSLAQACIQVLTSEMNENVPKVSNN
EEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
TVDIYKFDESILGSDSPDPLRFAFVKKHSAHAGGSSMPVPPPQQGDILSRITSETTTKN
EEEEEECCHHHCCCCCCCCCEEHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCC
NKRCLYIHVPFCRVRCTFCNFFQNAASRKLVDEYFDALMKELKEKAAMPWTQSGIFHAVY
CCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEE
IGGGTPTDLAPEQVRILGQAIHDYFPLAADCEITLEGRINRFSDELYQGALDGGFNRFSF
ECCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEECCCHHHHHHHHHHHHHCCCCHHHHH
GVQSFNTKVRRSAKRLDDREDVLNRVSELAKEDKIPVIIDLLYGLPYQTKDVLEQDLKDF
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCHHHHHHHHHHHH
MSTGAHGLDLYQLVVGGTAPMLNLVEKGKMSPPATTPEKAGMYELGAKFMAEHHMKQLSV
HHCCCCCHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCC
NHWAIDNRERSLYNSLAKTYAEVLPVGCGAGGNIGGYGMMQHRTLDTYVKAVNEGQSTIA
CHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHH
MMMKQSSLEPLFAALKSGFDRGIVQASKLPAFMGEDTFNFLMPLFKVWHDKGLVELQERS
HHHHHHCCHHHHHHHHCCHHHHHHHHHHCCHHHCCCHHHHHHHHHHHHHCCCCCEECCCC
LVLTLAGSFWAVSLAQACIQVLTSEMNENVPKVSNN
EEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8757728; 8969508; 9384377; 1339421 [H]