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The map label for this gene is pyrC

Identifier: 156976454

GI number: 156976454

Start: 480853

End: 481881

Strand: Reverse

Name: pyrC

Synonym: VIBHAR_05227

Alternate gene names: 156976454

Gene position: 481881-480853 (Counterclockwise)

Preceding gene: 156976455

Following gene: 156976452

Centisome position: 21.86

GC content: 47.52

Gene sequence:

>1029_bases
ATGACAACACTGACTATTACTCGTCCTGACGATTGGCACGTTCACTTACGCGATGGTGAAGTTCTAAAAGATACCGTTCG
CGATATTAGCCGTTACAACGGTCGAGCACTGATCATGCCGAACACCGTCCCACCTGTTACCAATACTGAAATGGCGCTAG
CCTACCGTGATCGAATCCTTGCAGAGCAGCACGGTGAGCAATTCGAGCCTCTTATGGCGCTGTACCTAACAGACAACACT
ACACCAGACGAAATCCGCAAAGCGAAAGCAACAGGAAAAATTGTTGCAGCAAAGCTTTATCCTGCGGGCGCAACCACCAA
CTCTGATTCTGGCGTAACCGACGCAAAGAACATCTACCACGTTTTTGAAGCGATGGAAGAAGTTGGCATGCTGCTTTTGG
TTCATGGCGAAGTGACCCACAACCACGTTGATATTTTTGATCGTGAGAAGGAATTCCTAGACACGGTTCTGGCTCCTATC
GTGAACGACTTCCCCAACCTAAAAATCGTTCTTGAGCACATCACGACTTCCGATGCGGCTAATTTCGTAAACAACGCTTC
TGACAATGTTGCAGCAACGATTACCGCTCACCACCTGCTGTTCAACCGTAATCACATGCTAGTCGGTGGTATTAAGCCAC
ACTTCTACTGCTTGCCAATTCTTAAGCGTAATACCCACCAACAAGCGCTTATCGAAGCAGCAACGAGCGGTAGCAAGAAA
TTCTTCCTAGGTACAGATTCAGCACCACACGCAAAAGGCGCAAAAGAGTCGGCTTGTGGTTGTGCAGGCTCTTACACAGC
ACACGCAGCGCTAGAACTCTATGCAGAAGTTTTTGAGAATGAAGGCAAGCTAGAAAACCTAGAAGCATTCGCTAGTTTGA
ATGGCCCTGATTTTTACGGTATCGCTCGTAACACAGATACTGTGACGCTAGAAAAAGCATCTTGGGATGTACCAGAAACG
ATGCCGTTTGGTAGCGACATCGTTGTGCCTATCCGCGCAAATGAAAAGATTGAGTGGGAAGTGAAGTAA

Upstream 100 bases:

>100_bases
AATTCCACTATCATCCTGTCGCCTAAGCGCAAGCGTTTGCTCTTGAGGTAAATGCAGCGTTTAGGCTCCAAACATAACTT
TTAAATGGTGGGAGCAATTA

Downstream 100 bases:

>100_bases
GCACGCTCACAAAATAAAAAAGGGTGATGGCACCATATTCGATATGGAAAGGCATGACCCTTTTCTTTTGGCTCCGATAA
AACCTTATGTCTGCTGTCTT

Product: dihydroorotase

Products: NA

Alternate protein names: DHOase

Number of amino acids: Translated: 342; Mature: 341

Protein sequence:

>342_residues
MTTLTITRPDDWHVHLRDGEVLKDTVRDISRYNGRALIMPNTVPPVTNTEMALAYRDRILAEQHGEQFEPLMALYLTDNT
TPDEIRKAKATGKIVAAKLYPAGATTNSDSGVTDAKNIYHVFEAMEEVGMLLLVHGEVTHNHVDIFDREKEFLDTVLAPI
VNDFPNLKIVLEHITTSDAANFVNNASDNVAATITAHHLLFNRNHMLVGGIKPHFYCLPILKRNTHQQALIEAATSGSKK
FFLGTDSAPHAKGAKESACGCAGSYTAHAALELYAEVFENEGKLENLEAFASLNGPDFYGIARNTDTVTLEKASWDVPET
MPFGSDIVVPIRANEKIEWEVK

Sequences:

>Translated_342_residues
MTTLTITRPDDWHVHLRDGEVLKDTVRDISRYNGRALIMPNTVPPVTNTEMALAYRDRILAEQHGEQFEPLMALYLTDNT
TPDEIRKAKATGKIVAAKLYPAGATTNSDSGVTDAKNIYHVFEAMEEVGMLLLVHGEVTHNHVDIFDREKEFLDTVLAPI
VNDFPNLKIVLEHITTSDAANFVNNASDNVAATITAHHLLFNRNHMLVGGIKPHFYCLPILKRNTHQQALIEAATSGSKK
FFLGTDSAPHAKGAKESACGCAGSYTAHAALELYAEVFENEGKLENLEAFASLNGPDFYGIARNTDTVTLEKASWDVPET
MPFGSDIVVPIRANEKIEWEVK
>Mature_341_residues
TTLTITRPDDWHVHLRDGEVLKDTVRDISRYNGRALIMPNTVPPVTNTEMALAYRDRILAEQHGEQFEPLMALYLTDNTT
PDEIRKAKATGKIVAAKLYPAGATTNSDSGVTDAKNIYHVFEAMEEVGMLLLVHGEVTHNHVDIFDREKEFLDTVLAPIV
NDFPNLKIVLEHITTSDAANFVNNASDNVAATITAHHLLFNRNHMLVGGIKPHFYCLPILKRNTHQQALIEAATSGSKKF
FLGTDSAPHAKGAKESACGCAGSYTAHAALELYAEVFENEGKLENLEAFASLNGPDFYGIARNTDTVTLEKASWDVPETM
PFGSDIVVPIRANEKIEWEVK

Specific function: Pyrimidine biosynthesis; third step. [C]

COG id: COG0418

COG function: function code F; Dihydroorotase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DHOase family. Type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1787301, Length=340, Percent_Identity=54.4117647058823, Blast_Score=381, Evalue=1e-107,
Organism=Saccharomyces cerevisiae, GI6323452, Length=357, Percent_Identity=35.8543417366947, Blast_Score=196, Evalue=6e-51,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): PYRC_VIBHB (A7N655)

Other databases:

- EMBL:   CP000790
- RefSeq:   YP_001447360.1
- ProteinModelPortal:   A7N655
- SMR:   A7N655
- STRING:   A7N655
- GeneID:   5557190
- GenomeReviews:   CP000790_GR
- KEGG:   vha:VIBHAR_05227
- NMPDR:   fig|338187.4.peg.3055
- eggNOG:   COG0418
- HOGENOM:   HBG628648
- OMA:   KESACGC
- ProtClustDB:   PRK05451
- HAMAP:   MF_00219
- InterPro:   IPR006680
- InterPro:   IPR004721
- InterPro:   IPR002195
- PIRSF:   PIRSF001237
- TIGRFAMs:   TIGR00856

Pfam domain/function: PF01979 Amidohydro_1

EC number: =3.5.2.3

Molecular weight: Translated: 37798; Mature: 37667

Theoretical pI: Translated: 5.37; Mature: 5.37

Prosite motif: PS00482 DIHYDROOROTASE_1; PS00483 DIHYDROOROTASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTLTITRPDDWHVHLRDGEVLKDTVRDISRYNGRALIMPNTVPPVTNTEMALAYRDRIL
CCEEEEECCCCCEEEEECCHHHHHHHHHHHHCCCEEEEECCCCCCCCCCHHHHHHHHHHH
AEQHGEQFEPLMALYLTDNTTPDEIRKAKATGKIVAAKLYPAGATTNSDSGVTDAKNIYH
HHHCCCCCCCEEEEEEECCCCHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCHHHHHHH
VFEAMEEVGMLLLVHGEVTHNHVDIFDREKEFLDTVLAPIVNDFPNLKIVLEHITTSDAA
HHHHHHHCCEEEEEECCCCCCEECEECCHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHH
NFVNNASDNVAATITAHHLLFNRNHMLVGGIKPHFYCLPILKRNTHQQALIEAATSGSKK
HHHCCCCCCEEEEEEEEEEEEECCEEEEECCCCCEEEEEHHHCCCHHHHHHHHHCCCCCE
FFLGTDSAPHAKGAKESACGCAGSYTAHAALELYAEVFENEGKLENLEAFASLNGPDFYG
EEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCEEE
IARNTDTVTLEKASWDVPETMPFGSDIVVPIRANEKIEWEVK
EECCCCEEEEECCCCCCCCCCCCCCCEEEEEECCCEEEEEEC
>Mature Secondary Structure 
TTLTITRPDDWHVHLRDGEVLKDTVRDISRYNGRALIMPNTVPPVTNTEMALAYRDRIL
CEEEEECCCCCEEEEECCHHHHHHHHHHHHCCCEEEEECCCCCCCCCCHHHHHHHHHHH
AEQHGEQFEPLMALYLTDNTTPDEIRKAKATGKIVAAKLYPAGATTNSDSGVTDAKNIYH
HHHCCCCCCCEEEEEEECCCCHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCHHHHHHH
VFEAMEEVGMLLLVHGEVTHNHVDIFDREKEFLDTVLAPIVNDFPNLKIVLEHITTSDAA
HHHHHHHCCEEEEEECCCCCCEECEECCHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHH
NFVNNASDNVAATITAHHLLFNRNHMLVGGIKPHFYCLPILKRNTHQQALIEAATSGSKK
HHHCCCCCCEEEEEEEEEEEEECCEEEEECCCCCEEEEEHHHCCCHHHHHHHHHCCCCCE
FFLGTDSAPHAKGAKESACGCAGSYTAHAALELYAEVFENEGKLENLEAFASLNGPDFYG
EEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCEEE
IARNTDTVTLEKASWDVPETMPFGSDIVVPIRANEKIEWEVK
EECCCCEEEEECCCCCCCCCCCCCCCEEEEEECCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA