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The map label for this gene is malE [H]

Identifier: 156976441

GI number: 156976441

Start: 470116

End: 471366

Strand: Direct

Name: malE [H]

Synonym: VIBHAR_05214

Alternate gene names: 156976441

Gene position: 470116-471366 (Clockwise)

Preceding gene: 156976439

Following gene: 156976442

Centisome position: 21.33

GC content: 46.6

Gene sequence:

>1251_bases
ATGATATGTGTCGAAATACGGCTGAACCTCAATGGACTAGCCCTACATCAAAAATATAAAAAGGATACGAACATGAAAAA
ATTAAGCGCTGTAGCACTGGGTACCCTTGTTGCTTTAGGTTCTTTTGGTGCTAACGCTGCCATCGAAGAAGGACAATTGA
CCATCTGGATCAACGGTGACAAAGGCTACAACGGTCTTGCAGAGGTTGGTAAGAAGTTTGAAGAAGACACTGGCATTAAA
GTAACAGTGGCTCATCCAGACGGCCTGCAAGACAAGTTCCCTCAAACTGCAGCGACAGGCGATGGTCCTGATATCGTATT
CTGGGCTCACGACCGTTTCGGTGGCTACGCAGAATCTGGTCTACTAGCTGAAATTAAACCATCTAAAGAAATCCAAGACG
GCATCGTTGACTTCGCATGGGATGCAGTACGTTACGATGGTAAACTGATCGGTTACCCAGTAGCGGTTGAGTCTCTATCT
CTAATCTACAACAAAGATCTTGTTCCAAACCCACCTAAGACATGGGAAGAAGTAGCAGCGCTAGACGCGAAACTTAAGAA
AGACGGCAAGTCTGCAATCATGTGGAACCTAAAAGAACCGTACTTCACATGGCCGTTAATGGCAGCTGACGGCGGCTACG
CATTCAAATACACATCTGAAGGCTACGACATCAAAGATGCAGGTATTGCGAAAGAAGGCGTGAAAGACGCAATGAGCTTC
GTGAAAGGCCTAGTAGACAAGGATGTCATCTCTGCGGATATGGATTACTCAGTATCTGAGTCTGCGTTCAACCAAGGCAC
TGCAGCAATGACTATTAACGGTCCATGGTCTTGGGGCAACATTGAGAAATCTGGCATCAACTATGGCGTAACAACGCTGC
CTAAGTTCCAAGGCCAGTCTTCTAAGCCATTCGTTGGTGTACTAACTGCTGGTATCAGCACTGCGTCGCCAAACAAAGAT
CTCGCGGTAGAGTTCATCGAGAACTACCTACTAACGAACGATGGTCTACGTATGGTTAACAACGACAAGCCACTAGGTGC
AGTAGCGCTTAACTCGTTCCAAAAAGAGCTAGATTCAGACACGCGTATCGCGGCGACAATGGACAACGCGATGAACGGCG
AAATCATGCCAAACATCCCACAAATGAACGCATTCTGGGGTGCAGCTAAGAACGCCATCATCAACGTGGTTGACGGTCGT
CAAACTGTAGATGCTGCTCTAGCAGATGCAGAAAAACAAATGACTAAATAA

Upstream 100 bases:

>100_bases
TGATTTGAGTCTCTAACCCTTGGCGATTCAGATCACGAAAATACGTCATAATTGCAAGGGCGTAGGGAGTAGGAGGATGA
GACACAATCGATAATTTCAG

Downstream 100 bases:

>100_bases
TTCAGTAATACCCTTTTTAAGGAGGGGGCAACTCCTCCTTACTTTTCTATATTTATCCTATATTCGCTAGCAGGTTCCCT
ATGCAGTCAGTTCAAGGTAC

Product: maltose ABC transporter periplasmic protein

Products: ADP; phosphate; maltose [Cytoplasm] [C]

Alternate protein names: MMBP; Maltodextrin-binding protein [H]

Number of amino acids: Translated: 416; Mature: 416

Protein sequence:

>416_residues
MICVEIRLNLNGLALHQKYKKDTNMKKLSAVALGTLVALGSFGANAAIEEGQLTIWINGDKGYNGLAEVGKKFEEDTGIK
VTVAHPDGLQDKFPQTAATGDGPDIVFWAHDRFGGYAESGLLAEIKPSKEIQDGIVDFAWDAVRYDGKLIGYPVAVESLS
LIYNKDLVPNPPKTWEEVAALDAKLKKDGKSAIMWNLKEPYFTWPLMAADGGYAFKYTSEGYDIKDAGIAKEGVKDAMSF
VKGLVDKDVISADMDYSVSESAFNQGTAAMTINGPWSWGNIEKSGINYGVTTLPKFQGQSSKPFVGVLTAGISTASPNKD
LAVEFIENYLLTNDGLRMVNNDKPLGAVALNSFQKELDSDTRIAATMDNAMNGEIMPNIPQMNAFWGAAKNAIINVVDGR
QTVDAALADAEKQMTK

Sequences:

>Translated_416_residues
MICVEIRLNLNGLALHQKYKKDTNMKKLSAVALGTLVALGSFGANAAIEEGQLTIWINGDKGYNGLAEVGKKFEEDTGIK
VTVAHPDGLQDKFPQTAATGDGPDIVFWAHDRFGGYAESGLLAEIKPSKEIQDGIVDFAWDAVRYDGKLIGYPVAVESLS
LIYNKDLVPNPPKTWEEVAALDAKLKKDGKSAIMWNLKEPYFTWPLMAADGGYAFKYTSEGYDIKDAGIAKEGVKDAMSF
VKGLVDKDVISADMDYSVSESAFNQGTAAMTINGPWSWGNIEKSGINYGVTTLPKFQGQSSKPFVGVLTAGISTASPNKD
LAVEFIENYLLTNDGLRMVNNDKPLGAVALNSFQKELDSDTRIAATMDNAMNGEIMPNIPQMNAFWGAAKNAIINVVDGR
QTVDAALADAEKQMTK
>Mature_416_residues
MICVEIRLNLNGLALHQKYKKDTNMKKLSAVALGTLVALGSFGANAAIEEGQLTIWINGDKGYNGLAEVGKKFEEDTGIK
VTVAHPDGLQDKFPQTAATGDGPDIVFWAHDRFGGYAESGLLAEIKPSKEIQDGIVDFAWDAVRYDGKLIGYPVAVESLS
LIYNKDLVPNPPKTWEEVAALDAKLKKDGKSAIMWNLKEPYFTWPLMAADGGYAFKYTSEGYDIKDAGIAKEGVKDAMSF
VKGLVDKDVISADMDYSVSESAFNQGTAAMTINGPWSWGNIEKSGINYGVTTLPKFQGQSSKPFVGVLTAGISTASPNKD
LAVEFIENYLLTNDGLRMVNNDKPLGAVALNSFQKELDSDTRIAATMDNAMNGEIMPNIPQMNAFWGAAKNAIINVVDGR
QTVDAALADAEKQMTK

Specific function: Involved in the high-affinity maltose membrane transport system malEFGK. Initial receptor for the active transport of and chemotaxis toward maltooligosaccharides [H]

COG id: COG2182

COG function: function code G; Maltose-binding periplasmic proteins/domains

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 1 family [H]

Homologues:

Organism=Escherichia coli, GI1790466, Length=397, Percent_Identity=67.2544080604534, Blast_Score=558, Evalue=1e-160,

Paralogues:

None

Copy number: 660 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 720 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 240 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006060
- InterPro:   IPR006059
- InterPro:   IPR006061 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 44988; Mature: 44988

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: PS01037 SBP_BACTERIAL_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MICVEIRLNLNGLALHQKYKKDTNMKKLSAVALGTLVALGSFGANAAIEEGQLTIWINGD
CEEEEEEECCCEEEEHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCEEECCEEEEEEECC
KGYNGLAEVGKKFEEDTGIKVTVAHPDGLQDKFPQTAATGDGPDIVFWAHDRFGGYAESG
CCCCHHHHHHHHHHCCCCCEEEEECCCCCHHHCCCCCCCCCCCEEEEEECCCCCCCCCCC
LLAEIKPSKEIQDGIVDFAWDAVRYDGKLIGYPVAVESLSLIYNKDLVPNPPKTWEEVAA
EEEECCCCHHHHHHHHHHHHHHHEECCEEEECCHHHHHHHHHCCCCCCCCCCHHHHHHHH
LDAKLKKDGKSAIMWNLKEPYFTWPLMAADGGYAFKYTSEGYDIKDAGIAKEGVKDAMSF
HHHHHHCCCCCEEEEECCCCCEEEEEEECCCCEEEEECCCCCCCCCCCCHHHHHHHHHHH
VKGLVDKDVISADMDYSVSESAFNQGTAAMTINGPWSWGNIEKSGINYGVTTLPKFQGQS
HHHHHHHHHHHCCCCCCCCHHHHCCCCEEEEECCCCCCCCEECCCCCCCCCCCCCCCCCC
SKPFVGVLTAGISTASPNKDLAVEFIENYLLTNDGLRMVNNDKPLGAVALNSFQKELDSD
CCCEEEEEECCCCCCCCCCHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHHCCC
TRIAATMDNAMNGEIMPNIPQMNAFWGAAKNAIINVVDGRQTVDAALADAEKQMTK
CEEEEECCCCCCCCCCCCCCCCHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MICVEIRLNLNGLALHQKYKKDTNMKKLSAVALGTLVALGSFGANAAIEEGQLTIWINGD
CEEEEEEECCCEEEEHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCEEECCEEEEEEECC
KGYNGLAEVGKKFEEDTGIKVTVAHPDGLQDKFPQTAATGDGPDIVFWAHDRFGGYAESG
CCCCHHHHHHHHHHCCCCCEEEEECCCCCHHHCCCCCCCCCCCEEEEEECCCCCCCCCCC
LLAEIKPSKEIQDGIVDFAWDAVRYDGKLIGYPVAVESLSLIYNKDLVPNPPKTWEEVAA
EEEECCCCHHHHHHHHHHHHHHHEECCEEEECCHHHHHHHHHCCCCCCCCCCHHHHHHHH
LDAKLKKDGKSAIMWNLKEPYFTWPLMAADGGYAFKYTSEGYDIKDAGIAKEGVKDAMSF
HHHHHHCCCCCEEEEECCCCCEEEEEEECCCCEEEEECCCCCCCCCCCCHHHHHHHHHHH
VKGLVDKDVISADMDYSVSESAFNQGTAAMTINGPWSWGNIEKSGINYGVTTLPKFQGQS
HHHHHHHHHHHCCCCCCCCHHHHCCCCEEEEECCCCCCCCEECCCCCCCCCCCCCCCCCC
SKPFVGVLTAGISTASPNKDLAVEFIENYLLTNDGLRMVNNDKPLGAVALNSFQKELDSD
CCCEEEEEECCCCCCCCCCHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHHCCC
TRIAATMDNAMNGEIMPNIPQMNAFWGAAKNAIINVVDGRQTVDAALADAEKQMTK
CEEEEECCCCCCCCCCCCCCCCHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; maltose [Periplasm]; H2O [C]

Specific reaction: ATP + maltose [Periplasm] + H2O = ADP + phosphate + maltose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]