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The map label for this gene is glxR [C]

Identifier: 156976418

GI number: 156976418

Start: 446850

End: 447728

Strand: Direct

Name: glxR [C]

Synonym: VIBHAR_05191

Alternate gene names: 156976418

Gene position: 446850-447728 (Clockwise)

Preceding gene: 156976416

Following gene: 156976435

Centisome position: 20.27

GC content: 47.78

Gene sequence:

>879_bases
ATGGAAAAACTAAGAGTAGCATTTATTGGTCTGGGTGTGATGGGATACCCAATGGCTGGTTACCTAAGTAAAGCGGGCTT
TGAAACCAAAGTATACAACCGCACAAAAGCGAAAGCCGACAAATGGGCTGAAGAATACCAAGGTGTGGCTTGTGAAACAC
CACGTGAAGCGGCAGAAGGCTGTGACATCGTTTTTACCTGTGTGGGGAATGATGATGACGTTCGTAGCGTTGTTTACGGT
GAAGAAGGCATGCTGGTTGGCTTAAAAGCGGATGCAGTGTTAGTGGATCACACGACGACTTCTGCAGACCTAGCGGTTGA
GCTGGCTGAAGCGTGTGTTAAGTACGGCAAGCACTTCATTGATGCGCCAGTTTCTGGCGGCCAAGCGGGTGCAGAAAATG
GTGTACTAACCATCATGTGTGGTGGTGAACAAAGCATTTTTGACCGTGTGTCTCCAGCGATGGATGTGTACGCGAAGCAG
ATGACGCTACTGGGTGAAAACGGTCAGGGCCAACGTTGTAAGATGGTAAACCAAATCTGTATTGGTGGTGTTTTACAAGG
TCTGAGTGAAGCTTTGCTACTGGCTCAAAAATCAGGCTTGGACATTGCTCAAGTTGTAGAAACGTTGAAGCATGGCGCTG
CAGGTTCATGGCAGATGGAAAACCGCGCAAATACGATGGCGGAAGATAAGTTCGATTTTGGCTTCGCTATCGATTGGATG
CGCAAAGATTTGGGTTTCTGTCTGCAAGAAGCAGAGCGAGTGGGTCTAGAGCTTCCATTGACGAAGAAAGTGGATGAGCA
ATACGCAGACCTTCAACGAGATGGTTTAGGTCGCATGGATACATCAGTACTGATCAAAGCGGTCGCTAAAAATCAGTAG

Upstream 100 bases:

>100_bases
GTAATGCTCTGAAATCTTTAGTAACATGTTTCAAATATTAACTGATTCAAATAGTAATAGTTTCAAAACGTCACTTATTT
CGAATTCAAGGAGGCTTGCA

Downstream 100 bases:

>100_bases
TTGATGAATCAGTAATAGATAAACAGTTCTTTGGTATCAAAGAGCTTAAAGAAATCTAAGTTAATCATAATGAATCTCCT
TGAGCATGGTTTGTGACTAG

Product: 3-hydroxyisobutyrate dehydrogenase

Products: 2-methyl-3-oxopropanoate; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 292; Mature: 292

Protein sequence:

>292_residues
MEKLRVAFIGLGVMGYPMAGYLSKAGFETKVYNRTKAKADKWAEEYQGVACETPREAAEGCDIVFTCVGNDDDVRSVVYG
EEGMLVGLKADAVLVDHTTTSADLAVELAEACVKYGKHFIDAPVSGGQAGAENGVLTIMCGGEQSIFDRVSPAMDVYAKQ
MTLLGENGQGQRCKMVNQICIGGVLQGLSEALLLAQKSGLDIAQVVETLKHGAAGSWQMENRANTMAEDKFDFGFAIDWM
RKDLGFCLQEAERVGLELPLTKKVDEQYADLQRDGLGRMDTSVLIKAVAKNQ

Sequences:

>Translated_292_residues
MEKLRVAFIGLGVMGYPMAGYLSKAGFETKVYNRTKAKADKWAEEYQGVACETPREAAEGCDIVFTCVGNDDDVRSVVYG
EEGMLVGLKADAVLVDHTTTSADLAVELAEACVKYGKHFIDAPVSGGQAGAENGVLTIMCGGEQSIFDRVSPAMDVYAKQ
MTLLGENGQGQRCKMVNQICIGGVLQGLSEALLLAQKSGLDIAQVVETLKHGAAGSWQMENRANTMAEDKFDFGFAIDWM
RKDLGFCLQEAERVGLELPLTKKVDEQYADLQRDGLGRMDTSVLIKAVAKNQ
>Mature_292_residues
MEKLRVAFIGLGVMGYPMAGYLSKAGFETKVYNRTKAKADKWAEEYQGVACETPREAAEGCDIVFTCVGNDDDVRSVVYG
EEGMLVGLKADAVLVDHTTTSADLAVELAEACVKYGKHFIDAPVSGGQAGAENGVLTIMCGGEQSIFDRVSPAMDVYAKQ
MTLLGENGQGQRCKMVNQICIGGVLQGLSEALLLAQKSGLDIAQVVETLKHGAAGSWQMENRANTMAEDKFDFGFAIDWM
RKDLGFCLQEAERVGLELPLTKKVDEQYADLQRDGLGRMDTSVLIKAVAKNQ

Specific function: Glyoxylate catabolism. [C]

COG id: COG2084

COG function: function code I; 3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-hydroxyisobutyrate dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI40556376, Length=264, Percent_Identity=28.7878787878788, Blast_Score=134, Evalue=9e-32,
Organism=Homo sapiens, GI23308751, Length=292, Percent_Identity=26.7123287671233, Blast_Score=103, Evalue=2e-22,
Organism=Escherichia coli, GI1786719, Length=285, Percent_Identity=36.140350877193, Blast_Score=180, Evalue=1e-46,
Organism=Escherichia coli, GI145693186, Length=282, Percent_Identity=37.2340425531915, Blast_Score=177, Evalue=8e-46,
Organism=Escherichia coli, GI1789092, Length=299, Percent_Identity=30.1003344481605, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI1790315, Length=285, Percent_Identity=29.4736842105263, Blast_Score=117, Evalue=6e-28,
Organism=Caenorhabditis elegans, GI17557316, Length=278, Percent_Identity=26.6187050359712, Blast_Score=93, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24655230, Length=287, Percent_Identity=31.7073170731707, Blast_Score=126, Evalue=1e-29,
Organism=Drosophila melanogaster, GI19922568, Length=287, Percent_Identity=31.7073170731707, Blast_Score=126, Evalue=1e-29,
Organism=Drosophila melanogaster, GI28574115, Length=280, Percent_Identity=26.7857142857143, Blast_Score=116, Evalue=1e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002204
- InterPro:   IPR015815
- InterPro:   IPR008927
- InterPro:   IPR006115
- InterPro:   IPR013328
- InterPro:   IPR016040
- InterPro:   IPR006183 [H]

Pfam domain/function: PF03446 NAD_binding_2 [H]

EC number: 1.1.1.31

Molecular weight: Translated: 31570; Mature: 31570

Theoretical pI: Translated: 4.58; Mature: 4.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
6.8 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
6.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKLRVAFIGLGVMGYPMAGYLSKAGFETKVYNRTKAKADKWAEEYQGVACETPREAAEG
CCCEEEEEEECCHHHCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCC
CDIVFTCVGNDDDVRSVVYGEEGMLVGLKADAVLVDHTTTSADLAVELAEACVKYGKHFI
CCEEEEEECCCCCHHHEEECCCCEEEEEECCEEEEECCCCHHHHHHHHHHHHHHHHHHEE
DAPVSGGQAGAENGVLTIMCGGEQSIFDRVSPAMDVYAKQMTLLGENGQGQRCKMVNQIC
CCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
IGGVLQGLSEALLLAQKSGLDIAQVVETLKHGAAGSWQMENRANTMAEDKFDFGFAIDWM
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCHHHHHH
RKDLGFCLQEAERVGLELPLTKKVDEQYADLQRDGLGRMDTSVLIKAVAKNQ
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MEKLRVAFIGLGVMGYPMAGYLSKAGFETKVYNRTKAKADKWAEEYQGVACETPREAAEG
CCCEEEEEEECCHHHCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCC
CDIVFTCVGNDDDVRSVVYGEEGMLVGLKADAVLVDHTTTSADLAVELAEACVKYGKHFI
CCEEEEEECCCCCHHHEEECCCCEEEEEECCEEEEECCCCHHHHHHHHHHHHHHHHHHEE
DAPVSGGQAGAENGVLTIMCGGEQSIFDRVSPAMDVYAKQMTLLGENGQGQRCKMVNQIC
CCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
IGGVLQGLSEALLLAQKSGLDIAQVVETLKHGAAGSWQMENRANTMAEDKFDFGFAIDWM
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCHHHHHH
RKDLGFCLQEAERVGLELPLTKKVDEQYADLQRDGLGRMDTSVLIKAVAKNQ
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-hydroxy-2-methylpropanoate; NAD+

Specific reaction: 3-hydroxy-2-methylpropanoate + NAD+ = 2-methyl-3-oxopropanoate + NADH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA