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The map label for this gene is yfgF [H]

Identifier: 156976207

GI number: 156976207

Start: 233758

End: 235962

Strand: Reverse

Name: yfgF [H]

Synonym: VIBHAR_04978

Alternate gene names: 156976207

Gene position: 235962-233758 (Counterclockwise)

Preceding gene: 156976208

Following gene: 156976198

Centisome position: 10.71

GC content: 45.49

Gene sequence:

>2205_bases
ATGAATAATGCTGCATCGGATCGCCGTATTAAAGTTAAAGCGATGCTGTTTCTGACGCTTGGTGTCACCTTCTCTGTGTT
TGCCGGATTTGCCCTTTCGCAGATGTTTGTCATAGAAAAAGATGCATTCCAGCTTATCTCGGTGGTGAGTTCGGTTATGG
TGGTGAGTTTTTTGCGCTATCAATGGCGAGCCTTGCCTGCAATTAACTTGAGCTTGTTCGCCTACTATGCCTTTACCGGT
CGAAGCCCTGAGCATGCCTTGTTGTATTCGCTCACCGTTCCGATCTTACCTTTTGCGTTTTCAAGCCTATTTCACTATCT
AAACAATCGCTCGCTACATCAACCAGCCGCACAGCGGATCAGCCTGTTTTTTGTCATTATGGGCTGTTTATTTCCTTTCG
CGAATACCATCAAAATCATGTTGGTGAATACCTTCGTTGATGGCCAGTTTTTAAGTCCTGATTTTCTATTTTATGCCACG
TTAGGCAATTACTTAACTCAACTGTTGGTAACACCTATTTTATACGTGCTGGTGAGCCTGTTGACGGATACCGATACCCA
GTCTTACCTCGCTTTGGATAAACGTTTAAGAATCTCTAATAGCCGCTGTCGAGGCTATTTAGCGTGGCTCGCGAGTTGCA
TCGCGATTTTCATTGCTGCCGCGCTTTCTTCCACCTCTTTCATGCTCAATTCGCTCAGCTTCTTCGCCGTTTTGCTTGTC
GTCGTTGGCTTGGCAAAACACGGCTTGATTCGGCCACTCTTTGTCGGCTCGCCATTTATCTTGTTGATAATCAATGACGG
TATCAGCCACTACAACCAAGCATTAATTGGAGACAAACAGTTCTACGGCTTATTGATCATCATTACTGTGCTTACCACCT
TGGCGTATTTACTGGGCGGCTACTCGGTAAAACTGTTTGAGATGACACAAAAGCAGATCCGCTCCGAGCGTATCGATCCA
TACTCTGGACTATCGAACATTGCTCAGCTGAAAGAAGACATCAGTCACCAATCTCGTTCTTTGCTCATCTACCTTGACTT
AACACCCACATTATCGATGTTGACCGATTTGGGCCATGAGGGAAAATCTCAGCTTATCATGCAACTCAGTGAGTTTTTGT
ACTCACGGAACAACAGCATAAATCGTTGTTATCGGCCACCTTTCTCAACGGGTCTACTGAGCTTTACCCATTACTCAGAT
ACCGCAGAAGAGGACCTTAACGAGGTCGCGGACTACTTAGAAAGCTTCCAATTTTATTGGCGAGGTACATCGGTTGCACT
AGTCTCCCCAACCATTTTCTGCACGCTCGTCAGCCAAGATCAGGACATTGAGAAAGTGGTGTCGATTCTGTGCGATCAAC
CGCCTCAAAGTGATAGCAGTATCCATTGGATTAAGATCCAATCTTTCGAAATGGACCGCGTGGACAAACTGAACCACATT
CAACAAGTGTTTAAGCGCGAACAATTTGAGTTGTTCTGCCAACCATACATGAAGCTTTCTGAATCTGAGCCGTCGAAACA
CAGCTTTGAAGTACTCCTTCGCCTGAAAGAGCAAGATGGGCAAGTTCTACCACCTTCGGCATTTTTCCCATTGATTAATC
AATTTGGTTTAGAGCTCAAGCTCGATCACTGGGTCATTCTCAATACCTTCCGAATGCTGAGTGACAAGGTGAAGAATTGG
AATAACATTGGTCACTGCGCCATCAACTTGACCGCCAAAACACTCAGTGCCGAGAACCTTGCAGTGCGCATCATTGAAAG
TGCGCAAAGCTTTTCGATTCCGCTGAACAAGATTTGCTTTGAGATCACCGAATCATCCGCACTCATCAATGAGCAACAAG
CCATTGCAAACTTGAGCCACTTACGAGATCACGGCTGTAAGATTGCGATAGACGACTTTGGAACAGGCTACGCTAGCTTC
GCGTACTTGCGCAGATTGCCATTGGATATTCTTAAAATTGAAGGGGCGTTTGTTCGTGAGATCACGCAGAGTGAGACGGA
CCGTTTGATCGTCAGCTCCATTGTAACGGTCGCAAAAGAAATGCATTTAGAGACGGTGGCAGAATTTGTCGAGAGCACTA
CACACATAGCGATATTAAGAAACTTGGGCATCGACTTTGCGCAAGGTTATGGCGTCGCGAAACCTATGCCTTTGAGCAAA
TGTTTAGATGAGCTATTTGAAGTTGAACACCTCAATCAGAGCTGA

Upstream 100 bases:

>100_bases
ACTCTTTAGAATAAAAACCCTATTTATCGAAATTTTCCTATTCATTCCATGCCGATAATCCTGTTATTATCGGCTACCCA
ACCACTTTCAGATCAAACTC

Downstream 100 bases:

>100_bases
GCGCACTATAAGACTTTGCGCTTTAATCGAGAGTTGATGATATCTTCAATCAGCTTTTGTTCTTTCTTGGCTTTTTCGTA
GTTTTGTTTAGCACGCCAAC

Product: diguanylate cyclase/phosphodiesterase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 734; Mature: 734

Protein sequence:

>734_residues
MNNAASDRRIKVKAMLFLTLGVTFSVFAGFALSQMFVIEKDAFQLISVVSSVMVVSFLRYQWRALPAINLSLFAYYAFTG
RSPEHALLYSLTVPILPFAFSSLFHYLNNRSLHQPAAQRISLFFVIMGCLFPFANTIKIMLVNTFVDGQFLSPDFLFYAT
LGNYLTQLLVTPILYVLVSLLTDTDTQSYLALDKRLRISNSRCRGYLAWLASCIAIFIAAALSSTSFMLNSLSFFAVLLV
VVGLAKHGLIRPLFVGSPFILLIINDGISHYNQALIGDKQFYGLLIIITVLTTLAYLLGGYSVKLFEMTQKQIRSERIDP
YSGLSNIAQLKEDISHQSRSLLIYLDLTPTLSMLTDLGHEGKSQLIMQLSEFLYSRNNSINRCYRPPFSTGLLSFTHYSD
TAEEDLNEVADYLESFQFYWRGTSVALVSPTIFCTLVSQDQDIEKVVSILCDQPPQSDSSIHWIKIQSFEMDRVDKLNHI
QQVFKREQFELFCQPYMKLSESEPSKHSFEVLLRLKEQDGQVLPPSAFFPLINQFGLELKLDHWVILNTFRMLSDKVKNW
NNIGHCAINLTAKTLSAENLAVRIIESAQSFSIPLNKICFEITESSALINEQQAIANLSHLRDHGCKIAIDDFGTGYASF
AYLRRLPLDILKIEGAFVREITQSETDRLIVSSIVTVAKEMHLETVAEFVESTTHIAILRNLGIDFAQGYGVAKPMPLSK
CLDELFEVEHLNQS

Sequences:

>Translated_734_residues
MNNAASDRRIKVKAMLFLTLGVTFSVFAGFALSQMFVIEKDAFQLISVVSSVMVVSFLRYQWRALPAINLSLFAYYAFTG
RSPEHALLYSLTVPILPFAFSSLFHYLNNRSLHQPAAQRISLFFVIMGCLFPFANTIKIMLVNTFVDGQFLSPDFLFYAT
LGNYLTQLLVTPILYVLVSLLTDTDTQSYLALDKRLRISNSRCRGYLAWLASCIAIFIAAALSSTSFMLNSLSFFAVLLV
VVGLAKHGLIRPLFVGSPFILLIINDGISHYNQALIGDKQFYGLLIIITVLTTLAYLLGGYSVKLFEMTQKQIRSERIDP
YSGLSNIAQLKEDISHQSRSLLIYLDLTPTLSMLTDLGHEGKSQLIMQLSEFLYSRNNSINRCYRPPFSTGLLSFTHYSD
TAEEDLNEVADYLESFQFYWRGTSVALVSPTIFCTLVSQDQDIEKVVSILCDQPPQSDSSIHWIKIQSFEMDRVDKLNHI
QQVFKREQFELFCQPYMKLSESEPSKHSFEVLLRLKEQDGQVLPPSAFFPLINQFGLELKLDHWVILNTFRMLSDKVKNW
NNIGHCAINLTAKTLSAENLAVRIIESAQSFSIPLNKICFEITESSALINEQQAIANLSHLRDHGCKIAIDDFGTGYASF
AYLRRLPLDILKIEGAFVREITQSETDRLIVSSIVTVAKEMHLETVAEFVESTTHIAILRNLGIDFAQGYGVAKPMPLSK
CLDELFEVEHLNQS
>Mature_734_residues
MNNAASDRRIKVKAMLFLTLGVTFSVFAGFALSQMFVIEKDAFQLISVVSSVMVVSFLRYQWRALPAINLSLFAYYAFTG
RSPEHALLYSLTVPILPFAFSSLFHYLNNRSLHQPAAQRISLFFVIMGCLFPFANTIKIMLVNTFVDGQFLSPDFLFYAT
LGNYLTQLLVTPILYVLVSLLTDTDTQSYLALDKRLRISNSRCRGYLAWLASCIAIFIAAALSSTSFMLNSLSFFAVLLV
VVGLAKHGLIRPLFVGSPFILLIINDGISHYNQALIGDKQFYGLLIIITVLTTLAYLLGGYSVKLFEMTQKQIRSERIDP
YSGLSNIAQLKEDISHQSRSLLIYLDLTPTLSMLTDLGHEGKSQLIMQLSEFLYSRNNSINRCYRPPFSTGLLSFTHYSD
TAEEDLNEVADYLESFQFYWRGTSVALVSPTIFCTLVSQDQDIEKVVSILCDQPPQSDSSIHWIKIQSFEMDRVDKLNHI
QQVFKREQFELFCQPYMKLSESEPSKHSFEVLLRLKEQDGQVLPPSAFFPLINQFGLELKLDHWVILNTFRMLSDKVKNW
NNIGHCAINLTAKTLSAENLAVRIIESAQSFSIPLNKICFEITESSALINEQQAIANLSHLRDHGCKIAIDDFGTGYASF
AYLRRLPLDILKIEGAFVREITQSETDRLIVSSIVTVAKEMHLETVAEFVESTTHIAILRNLGIDFAQGYGVAKPMPLSK
CLDELFEVEHLNQS

Specific function: Truncated proteins consisting of the GGDEF/EAL domains (residues 319-747) or of the EAL domain alone (481-747) have c-di- GMP phosphodiesterase activity. They do not have diguanylate cyclase activity. Cyclic-di-GMP is a second messenger which controls cel

COG id: COG2200

COG function: function code T; FOG: EAL domain

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 EAL domain [H]

Homologues:

Organism=Escherichia coli, GI1788849, Length=433, Percent_Identity=30.0230946882217, Blast_Score=190, Evalue=3e-49,
Organism=Escherichia coli, GI87082096, Length=625, Percent_Identity=27.2, Blast_Score=170, Evalue=2e-43,
Organism=Escherichia coli, GI1790496, Length=238, Percent_Identity=31.9327731092437, Blast_Score=131, Evalue=1e-31,
Organism=Escherichia coli, GI1787541, Length=242, Percent_Identity=30.1652892561983, Blast_Score=125, Evalue=7e-30,
Organism=Escherichia coli, GI226510982, Length=268, Percent_Identity=29.1044776119403, Blast_Score=124, Evalue=1e-29,
Organism=Escherichia coli, GI87081743, Length=208, Percent_Identity=30.7692307692308, Blast_Score=107, Evalue=3e-24,
Organism=Escherichia coli, GI87081980, Length=235, Percent_Identity=30.2127659574468, Blast_Score=107, Evalue=3e-24,
Organism=Escherichia coli, GI1788502, Length=251, Percent_Identity=29.4820717131474, Blast_Score=106, Evalue=4e-24,
Organism=Escherichia coli, GI87081921, Length=238, Percent_Identity=26.0504201680672, Blast_Score=102, Evalue=1e-22,
Organism=Escherichia coli, GI1786507, Length=215, Percent_Identity=29.7674418604651, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI87081845, Length=225, Percent_Identity=28.4444444444444, Blast_Score=98, Evalue=2e-21,
Organism=Escherichia coli, GI1787055, Length=214, Percent_Identity=28.9719626168224, Blast_Score=72, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000160
- InterPro:   IPR001633
- InterPro:   IPR007895 [H]

Pfam domain/function: PF00563 EAL; PF05231 MASE1 [H]

EC number: =3.1.4.52 [H]

Molecular weight: Translated: 82900; Mature: 82900

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS50883 EAL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNAASDRRIKVKAMLFLTLGVTFSVFAGFALSQMFVIEKDAFQLISVVSSVMVVSFLRY
CCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QWRALPAINLSLFAYYAFTGRSPEHALLYSLTVPILPFAFSSLFHYLNNRSLHQPAAQRI
HHHCCCCCHHHHEEEEEECCCCCCCEEEHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHH
SLFFVIMGCLFPFANTIKIMLVNTFVDGQFLSPDFLFYATLGNYLTQLLVTPILYVLVSL
HHHHHHHHHHHHHHCCEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LTDTDTQSYLALDKRLRISNSRCRGYLAWLASCIAIFIAAALSSTSFMLNSLSFFAVLLV
HHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VVGLAKHGLIRPLFVGSPFILLIINDGISHYNQALIGDKQFYGLLIIITVLTTLAYLLGG
HHHHHHCCCCCEEECCCCEEEEEEECCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCC
YSVKLFEMTQKQIRSERIDPYSGLSNIAQLKEDISHQSRSLLIYLDLTPTLSMLTDLGHE
CCEEHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHCCCC
GKSQLIMQLSEFLYSRNNSINRCYRPPFSTGLLSFTHYSDTAEEDLNEVADYLESFQFYW
CHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHH
RGTSVALVSPTIFCTLVSQDQDIEKVVSILCDQPPQSDSSIHWIKIQSFEMDRVDKLNHI
CCCEEEEECCEEEEEEECCCCCHHHHHHHHCCCCCCCCCCEEEEEEECCCCHHHHHHHHH
QQVFKREQFELFCQPYMKLSESEPSKHSFEVLLRLKEQDGQVLPPSAFFPLINQFGLELK
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCEEE
LDHWVILNTFRMLSDKVKNWNNIGHCAINLTAKTLSAENLAVRIIESAQSFSIPLNKICF
ECCCHHHHHHHHHHHHHHCCCCCCEEEEEEEEHHCCCCHHHHHHHHHHHHCCCCHHHHHH
EITESSALINEQQAIANLSHLRDHGCKIAIDDFGTGYASFAYLRRLPLDILKIEGAFVRE
HHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHCCHHHEEECCHHHHH
ITQSETDRLIVSSIVTVAKEMHLETVAEFVESTTHIAILRNLGIDFAQGYGVAKPMPLSK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCHHH
CLDELFEVEHLNQS
HHHHHHHHHHCCCC
>Mature Secondary Structure
MNNAASDRRIKVKAMLFLTLGVTFSVFAGFALSQMFVIEKDAFQLISVVSSVMVVSFLRY
CCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QWRALPAINLSLFAYYAFTGRSPEHALLYSLTVPILPFAFSSLFHYLNNRSLHQPAAQRI
HHHCCCCCHHHHEEEEEECCCCCCCEEEHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHH
SLFFVIMGCLFPFANTIKIMLVNTFVDGQFLSPDFLFYATLGNYLTQLLVTPILYVLVSL
HHHHHHHHHHHHHHCCEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LTDTDTQSYLALDKRLRISNSRCRGYLAWLASCIAIFIAAALSSTSFMLNSLSFFAVLLV
HHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VVGLAKHGLIRPLFVGSPFILLIINDGISHYNQALIGDKQFYGLLIIITVLTTLAYLLGG
HHHHHHCCCCCEEECCCCEEEEEEECCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCC
YSVKLFEMTQKQIRSERIDPYSGLSNIAQLKEDISHQSRSLLIYLDLTPTLSMLTDLGHE
CCEEHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHCCCC
GKSQLIMQLSEFLYSRNNSINRCYRPPFSTGLLSFTHYSDTAEEDLNEVADYLESFQFYW
CHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHH
RGTSVALVSPTIFCTLVSQDQDIEKVVSILCDQPPQSDSSIHWIKIQSFEMDRVDKLNHI
CCCEEEEECCEEEEEEECCCCCHHHHHHHHCCCCCCCCCCEEEEEEECCCCHHHHHHHHH
QQVFKREQFELFCQPYMKLSESEPSKHSFEVLLRLKEQDGQVLPPSAFFPLINQFGLELK
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCEEE
LDHWVILNTFRMLSDKVKNWNNIGHCAINLTAKTLSAENLAVRIIESAQSFSIPLNKICF
ECCCHHHHHHHHHHHHHHCCCCCCEEEEEEEEHHCCCCHHHHHHHHHHHHCCCCHHHHHH
EITESSALINEQQAIANLSHLRDHGCKIAIDDFGTGYASFAYLRRLPLDILKIEGAFVRE
HHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHCCHHHEEECCHHHHH
ITQSETDRLIVSSIVTVAKEMHLETVAEFVESTTHIAILRNLGIDFAQGYGVAKPMPLSK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCHHH
CLDELFEVEHLNQS
HHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]