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The map label for this gene is purT

Identifier: 156938203

GI number: 156938203

Start: 1273947

End: 1275155

Strand: Direct

Name: purT

Synonym: Igni_1416

Alternate gene names: 156938203

Gene position: 1273947-1275155 (Clockwise)

Preceding gene: 156938202

Following gene: 156938205

Centisome position: 98.18

GC content: 57.49

Gene sequence:

>1209_bases
TTGAGAGAAGTTTTGGGTACTGCCCTCACGTCGTCTTCCCAGAAAGCCCTATTGCTCGGTTCGGGAGAGCTCGGCAAAGA
GGTAGTTATAGAGCTTCAGAGGCTGGGCGTGGAAGTAGTTGCAGTAGACCGTTACGACCGCGCCCCAGCTATGCACGTAG
CCCATCGGAGGTACGTAATAGACATGCTAGACTACGACCAAGTGGTCGACGTGGTTCGCAGGGAGCGCCCAGACGTCATA
ATACCCGAAGTGGAGGCGATTAACACGGACGCCCTCGTGGACTTGGAAAAGGAAGGTTACTTCGTAGTCCCTAACGCGAG
GGCGGTAAAGATAACCATGAACCGGATTGAGCTGAGGAGGCTAGCGGCAGAAAAGGTAGGGGTTCCCACCACTCGTTACG
CGTTCGCCCACAACGAAGACGAGGCCGCAGAAGCTTGCGAGAAAGTCGGCTACCCGTGCTTGATAAAGCCCGAGATGAGC
TCCTCCGGCCACGGACACACCTTAGCCTCTTCGCCGGAGGAGGCCCGGGAGGGATTTAAGAGGGCCTTGAAGGAGGCCCG
GGGCAAGAGCGAGAGGGCCATAGTAGAAGAGTTCGTTGAAATAGATAGGGAACTCACCGCCCTAACGTACAGGCACGACA
CGGGTAATGGAATAGAAACAGTCCCCCTTCCCCCAGTAGAACACAAGAGGCCCAAGGGCATCTACTACTATTACGAGTCT
TGGCACCCCGCTACCGTCAACGACGAAGTGGTGAAGAAGGCTAAGGACATAGCAGTAAAGGTGGTAAACGAGTTAGGGGG
CTTAGGCATCTTCGGGGTTGAGATATTAGTTACCAAGGACGGGAGGGTACTGTTCAGCGAGGTCTCTCCCCGCCCTCACG
ACACCGGCCTGGTCACCTTAGCTAGCATGGAGCTGAGTGAGTTCGCAATTCACGCCCGCGCGGTCCTCGGCCTCCCCGTC
CCTGAGCCCAAGTTACTAACTCCCGCGGCCTCTCGAGTGGTGCTGGCAGAGGAGGCCTTGGAGCCCCCCTGCTTGAAGGG
GGTCGGCGAAGCGCTTAAGGTGAAGGGCGTCCAGCTGAGGTGGTTCGCCAAGCCGCGCAGTTACAAGGAGAGGAGGATGG
GAGTGTTACTAGCGACCGGCTCCACGGTGGAGGAGGCGTTGGAGAGGGTTAGGAGGGCCGCCGGGTTCCTAAAAGTGGTT
AAGTGTTAA

Upstream 100 bases:

>100_bases
CCAATGAGGAAGCCCGAAAAGTCCGAGGAGGGTGAGGTGAGTGCGGGGCTGGGCTGAGGGAAAATATTATCCTCACGGCC
GCTTTCCTTCATGAGGAACC

Downstream 100 bases:

>100_bases
GGTCCTCCTTTTGGGTTATGAAGTTCACCCTTCGCTCGACTTCTTATGGAACCTCGCGTATATCATTGCGTGGTCTTTGT
CGAAGGGGTCTAAGTGCACT

Product: phosphoribosylglycinamide formyltransferase 2

Products: 5'-Phosphoribosyl-N-Formylglycinamide; Pyrophosphate; acetylphosphate; ADP [C]

Alternate protein names: GART 2; 5'-phosphoribosylglycinamide transformylase 2; Formate-dependent GAR transformylase; GAR transformylase 2

Number of amino acids: Translated: 402; Mature: 402

Protein sequence:

>402_residues
MREVLGTALTSSSQKALLLGSGELGKEVVIELQRLGVEVVAVDRYDRAPAMHVAHRRYVIDMLDYDQVVDVVRRERPDVI
IPEVEAINTDALVDLEKEGYFVVPNARAVKITMNRIELRRLAAEKVGVPTTRYAFAHNEDEAAEACEKVGYPCLIKPEMS
SSGHGHTLASSPEEAREGFKRALKEARGKSERAIVEEFVEIDRELTALTYRHDTGNGIETVPLPPVEHKRPKGIYYYYES
WHPATVNDEVVKKAKDIAVKVVNELGGLGIFGVEILVTKDGRVLFSEVSPRPHDTGLVTLASMELSEFAIHARAVLGLPV
PEPKLLTPAASRVVLAEEALEPPCLKGVGEALKVKGVQLRWFAKPRSYKERRMGVLLATGSTVEEALERVRRAAGFLKVV
KC

Sequences:

>Translated_402_residues
MREVLGTALTSSSQKALLLGSGELGKEVVIELQRLGVEVVAVDRYDRAPAMHVAHRRYVIDMLDYDQVVDVVRRERPDVI
IPEVEAINTDALVDLEKEGYFVVPNARAVKITMNRIELRRLAAEKVGVPTTRYAFAHNEDEAAEACEKVGYPCLIKPEMS
SSGHGHTLASSPEEAREGFKRALKEARGKSERAIVEEFVEIDRELTALTYRHDTGNGIETVPLPPVEHKRPKGIYYYYES
WHPATVNDEVVKKAKDIAVKVVNELGGLGIFGVEILVTKDGRVLFSEVSPRPHDTGLVTLASMELSEFAIHARAVLGLPV
PEPKLLTPAASRVVLAEEALEPPCLKGVGEALKVKGVQLRWFAKPRSYKERRMGVLLATGSTVEEALERVRRAAGFLKVV
KC
>Mature_402_residues
MREVLGTALTSSSQKALLLGSGELGKEVVIELQRLGVEVVAVDRYDRAPAMHVAHRRYVIDMLDYDQVVDVVRRERPDVI
IPEVEAINTDALVDLEKEGYFVVPNARAVKITMNRIELRRLAAEKVGVPTTRYAFAHNEDEAAEACEKVGYPCLIKPEMS
SSGHGHTLASSPEEAREGFKRALKEARGKSERAIVEEFVEIDRELTALTYRHDTGNGIETVPLPPVEHKRPKGIYYYYES
WHPATVNDEVVKKAKDIAVKVVNELGGLGIFGVEILVTKDGRVLFSEVSPRPHDTGLVTLASMELSEFAIHARAVLGLPV
PEPKLLTPAASRVVLAEEALEPPCLKGVGEALKVKGVQLRWFAKPRSYKERRMGVLLATGSTVEEALERVRRAAGFLKVV
KC

Specific function: Catalyzes two reactions:the first one is the production of beta-formyl glycinamide ribonucleotide (GAR) from formate, ATP and beta GAR; the second, a side reaction, is the production of acetyl phosphate and ADP from acetate and ATP

COG id: COG0027

COG function: function code F; Formate-dependent phosphoribosylglycinamide formyltransferase (GAR transformylase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Escherichia coli, GI1788155, Length=396, Percent_Identity=50.5050505050505, Blast_Score=357, Evalue=1e-100,
Organism=Escherichia coli, GI1786216, Length=243, Percent_Identity=25.5144032921811, Blast_Score=65, Evalue=6e-12,
Organism=Saccharomyces cerevisiae, GI6324702, Length=388, Percent_Identity=23.9690721649485, Blast_Score=96, Evalue=9e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PURT_IGNH4 (A8ACE0)

Other databases:

- EMBL:   CP000816
- RefSeq:   YP_001435999.1
- ProteinModelPortal:   A8ACE0
- SMR:   A8ACE0
- STRING:   A8ACE0
- GeneID:   5561783
- GenomeReviews:   CP000816_GR
- KEGG:   iho:Igni_1416
- eggNOG:   arNOG04188
- HOGENOM:   HBG516369
- OMA:   TMNRKAI
- ProtClustDB:   PRK09288
- BioCyc:   IHOS453591:IGNI_1416-MONOMER
- HAMAP:   MF_01643
- InterPro:   IPR011761
- InterPro:   IPR003135
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR005862
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.40.50.20
- PANTHER:   PTHR23047:SF2

Pfam domain/function: PF02222 ATP-grasp; SSF52440 PreATP-grasp-like

EC number: 2.1.2.- [C]

Molecular weight: Translated: 44458; Mature: 44458

Theoretical pI: Translated: 6.86; Mature: 6.86

Prosite motif: PS50975 ATP_GRASP

Important sites: BINDING 83-83 BINDING 115-115 BINDING 156-156 BINDING 204-204 BINDING 274-274 BINDING 287-287 BINDING 294-294 BINDING 364-364

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MREVLGTALTSSSQKALLLGSGELGKEVVIELQRLGVEVVAVDRYDRAPAMHVAHRRYVI
CCHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHCCEEEEEECCCCCCCHHHHHHHHHHH
DMLDYDQVVDVVRRERPDVIIPEVEAINTDALVDLEKEGYFVVPNARAVKITMNRIELRR
HHCCHHHHHHHHHHCCCCEECCCHHCCCCCCEEEECCCCEEECCCCEEEEEEHHHHHHHH
LAAEKVGVPTTRYAFAHNEDEAAEACEKVGYPCLIKPEMSSSGHGHTLASSPEEAREGFK
HHHHHCCCCCEEEEEECCCHHHHHHHHHCCCCEEECCCCCCCCCCCCCCCCHHHHHHHHH
RALKEARGKSERAIVEEFVEIDRELTALTYRHDTGNGIETVPLPPVEHKRPKGIYYYYES
HHHHHHCCCCHHHHHHHHHHHHHHHEEEEEEECCCCCEEECCCCCCCCCCCCCEEEEECC
WHPATVNDEVVKKAKDIAVKVVNELGGLGIFGVEILVTKDGRVLFSEVSPRPHDTGLVTL
CCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEECCCCEEEEECCCCCCCCCEEEE
ASMELSEFAIHARAVLGLPVPEPKLLTPAASRVVLAEEALEPPCLKGVGEALKVKGVQLR
EEHHHHHHHHHHHHEECCCCCCCCCCCCCHHHEEEHHHHCCCHHHHHCCHHHEECCEEEE
WFAKPRSYKERRMGVLLATGSTVEEALERVRRAAGFLKVVKC
EEECCCCHHHHHCEEEEECCCHHHHHHHHHHHHHHHHEEECC
>Mature Secondary Structure
MREVLGTALTSSSQKALLLGSGELGKEVVIELQRLGVEVVAVDRYDRAPAMHVAHRRYVI
CCHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHCCEEEEEECCCCCCCHHHHHHHHHHH
DMLDYDQVVDVVRRERPDVIIPEVEAINTDALVDLEKEGYFVVPNARAVKITMNRIELRR
HHCCHHHHHHHHHHCCCCEECCCHHCCCCCCEEEECCCCEEECCCCEEEEEEHHHHHHHH
LAAEKVGVPTTRYAFAHNEDEAAEACEKVGYPCLIKPEMSSSGHGHTLASSPEEAREGFK
HHHHHCCCCCEEEEEECCCHHHHHHHHHCCCCEEECCCCCCCCCCCCCCCCHHHHHHHHH
RALKEARGKSERAIVEEFVEIDRELTALTYRHDTGNGIETVPLPPVEHKRPKGIYYYYES
HHHHHHCCCCHHHHHHHHHHHHHHHEEEEEEECCCCCEEECCCCCCCCCCCCCEEEEECC
WHPATVNDEVVKKAKDIAVKVVNELGGLGIFGVEILVTKDGRVLFSEVSPRPHDTGLVTL
CCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEECCCCEEEEECCCCCCCCCEEEE
ASMELSEFAIHARAVLGLPVPEPKLLTPAASRVVLAEEALEPPCLKGVGEALKVKGVQLR
EEHHHHHHHHHHHHEECCCCCCCCCCCCCHHHEEEHHHHCCCHHHHHCCHHHEECCEEEE
WFAKPRSYKERRMGVLLATGSTVEEALERVRRAAGFLKVVKC
EEECCCCHHHHHCEEEEECCCHHHHHHHHHHHHHHHHEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Formate; 5'-Phospho-Ribosylglycinamide; acetate; ATP [C]

Specific reaction: Formate + ATP + 5'-Phospho-Ribosylglycinamide = 5'-Phosphoribosyl-N-Formylglycinamide + ADP + Pyrophosphate. acetate + ATP = acetylphosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA