Click here to switch to the map view.

The map label for this gene is lysX [H]

Identifier: 156938190

GI number: 156938190

Start: 1264496

End: 1265353

Strand: Direct

Name: lysX [H]

Synonym: Igni_1403

Alternate gene names: 156938190

Gene position: 1264496-1265353 (Clockwise)

Preceding gene: 156938189

Following gene: 156938194

Centisome position: 97.45

GC content: 51.75

Gene sequence:

>858_bases
GTGAGGTGGTTCCACCTCGTTGTGAAGGTCTATTATGATTTGCCCCGTGTCGAAGAGAAGCTGATAATAAAGTCGTTAGA
GTCCCACGGACTTGAGACGGTTCCCGTCAACGTAACCAAGGAGCCGTTGCCCCTACAAGGCATAGAAGAACACGTACCAA
TAGTACGAGCCGTAAGCATGTTCAGATCTACTTACACCTCGGCTGTCCTCGAAGCAAACGGCATGAAACCCATAAACAGT
GCTTACACAATCATGTTCTCCGGAGACAAGGTTCTAACGTACTCAGCCCTGGCATCTCACGGGATACCCATACCCAAGAC
CGTGATAGCCCTCAACGGGGACTCTACCGAAAAGGCTTACGCCAGCGTGGGGTTCCCACTCGTGGACAAGCCCCCTATAG
GGAGCTGGGGGAGGCTGGTGAGCTTAGTAAGGGACTGGCACGAAGCCAAGATCGTAATAGAACATAGATCTATGATGAAC
AGCCCCCAAATGAAGGTACACATAGTTCAAGAATACGTCAAAATGCCGGAGAACAGAGACATAAGGTGTTTCGTAATTGG
GGGTAACTGCTTGGGCTGTATCTACCGCGTACCCAGCGAGGGCGAGTGGAGGTCCAACGTAGCCCTCGGAGGGAAGGTGA
GGGCCTTGAAGGACTCGACGGAGCCGTGCGAGCTGGCGGTAAAGGCCGCGGAGGCTCTGAAGGGAGAAGTGGTATCGATA
GATATCTTTGAAGGGAAGGAAGGATACTTAGTGAACGAGGTGAACGGCGTCCCGGAGTTTAAAGGGTTTATGAAGGCTAC
GGGTATTAATGTGGGAGACGAAATAGCGAAGTACGTCTACGAGAGGGTTAAGAGATGA

Upstream 100 bases:

>100_bases
GGCGCCACCCTGGAGGTCAAGAAGAGTGGGGACAAGGTGGAACTGAAGGTAGCCGAAGAAGTGGGAGAGGACTGGGGCCA
GTAGCGAGCAGGGGGTGGAG

Downstream 100 bases:

>100_bases
GTTTATCAAGCGGCCACCGCGCGGGGGCAAGTTTTTGTACGTGAAACAAACTCTAGGAACCCAGCTCCTCGAAACCCTTC
CTCAACCTTCTCCACGTCTC

Product: lysine biosynthesis enzyme LysX

Products: NA

Alternate protein names: AAA--lysW ligase lysX [H]

Number of amino acids: Translated: 285; Mature: 285

Protein sequence:

>285_residues
MRWFHLVVKVYYDLPRVEEKLIIKSLESHGLETVPVNVTKEPLPLQGIEEHVPIVRAVSMFRSTYTSAVLEANGMKPINS
AYTIMFSGDKVLTYSALASHGIPIPKTVIALNGDSTEKAYASVGFPLVDKPPIGSWGRLVSLVRDWHEAKIVIEHRSMMN
SPQMKVHIVQEYVKMPENRDIRCFVIGGNCLGCIYRVPSEGEWRSNVALGGKVRALKDSTEPCELAVKAAEALKGEVVSI
DIFEGKEGYLVNEVNGVPEFKGFMKATGINVGDEIAKYVYERVKR

Sequences:

>Translated_285_residues
MRWFHLVVKVYYDLPRVEEKLIIKSLESHGLETVPVNVTKEPLPLQGIEEHVPIVRAVSMFRSTYTSAVLEANGMKPINS
AYTIMFSGDKVLTYSALASHGIPIPKTVIALNGDSTEKAYASVGFPLVDKPPIGSWGRLVSLVRDWHEAKIVIEHRSMMN
SPQMKVHIVQEYVKMPENRDIRCFVIGGNCLGCIYRVPSEGEWRSNVALGGKVRALKDSTEPCELAVKAAEALKGEVVSI
DIFEGKEGYLVNEVNGVPEFKGFMKATGINVGDEIAKYVYERVKR
>Mature_285_residues
MRWFHLVVKVYYDLPRVEEKLIIKSLESHGLETVPVNVTKEPLPLQGIEEHVPIVRAVSMFRSTYTSAVLEANGMKPINS
AYTIMFSGDKVLTYSALASHGIPIPKTVIALNGDSTEKAYASVGFPLVDKPPIGSWGRLVSLVRDWHEAKIVIEHRSMMN
SPQMKVHIVQEYVKMPENRDIRCFVIGGNCLGCIYRVPSEGEWRSNVALGGKVRALKDSTEPCELAVKAAEALKGEVVSI
DIFEGKEGYLVNEVNGVPEFKGFMKATGINVGDEIAKYVYERVKR

Specific function: Catalyzes the ATP-dependent binding between the amino group of alpha-aminoadipate (AAA) and the gamma-carboxyl group of 'Glu-54' of lysW [H]

COG id: COG0189

COG function: function code HJ; Glutathione synthase/Ribosomal protein S6 modification enzyme (glutaminyl transferase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI164663810, Length=214, Percent_Identity=29.4392523364486, Blast_Score=77, Evalue=3e-14,
Organism=Homo sapiens, GI134254462, Length=213, Percent_Identity=30.5164319248826, Blast_Score=75, Evalue=8e-14,
Organism=Escherichia coli, GI1787076, Length=230, Percent_Identity=33.0434782608696, Blast_Score=110, Evalue=9e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013651
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR011870
- InterPro:   IPR016185
- InterPro:   IPR004666 [H]

Pfam domain/function: PF08443 RimK [H]

EC number: NA

Molecular weight: Translated: 31721; Mature: 31721

Theoretical pI: Translated: 7.98; Mature: 7.98

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRWFHLVVKVYYDLPRVEEKLIIKSLESHGLETVPVNVTKEPLPLQGIEEHVPIVRAVSM
CHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHH
FRSTYTSAVLEANGMKPINSAYTIMFSGDKVLTYSALASHGIPIPKTVIALNGDSTEKAY
HHHHHHHHHHHCCCCCCCCCEEEEEEECCEEEEEHHHHHCCCCCCCEEEEECCCCCHHHH
ASVGFPLVDKPPIGSWGRLVSLVRDWHEAKIVIEHRSMMNSPQMKVHIVQEYVKMPENRD
HHCCCCCCCCCCCCCHHHHHHHHHHHHHHEEEEEHHHHCCCCCHHHHHHHHHHHCCCCCC
IRCFVIGGNCLGCIYRVPSEGEWRSNVALGGKVRALKDSTEPCELAVKAAEALKGEVVSI
EEEEEECCCEEEEEEECCCCCCCCCCEEECCEEEEECCCCCHHHHHHHHHHHHCCCEEEE
DIFEGKEGYLVNEVNGVPEFKGFMKATGINVGDEIAKYVYERVKR
EEEECCCCEEEECCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MRWFHLVVKVYYDLPRVEEKLIIKSLESHGLETVPVNVTKEPLPLQGIEEHVPIVRAVSM
CHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHH
FRSTYTSAVLEANGMKPINSAYTIMFSGDKVLTYSALASHGIPIPKTVIALNGDSTEKAY
HHHHHHHHHHHCCCCCCCCCEEEEEEECCEEEEEHHHHHCCCCCCCEEEEECCCCCHHHH
ASVGFPLVDKPPIGSWGRLVSLVRDWHEAKIVIEHRSMMNSPQMKVHIVQEYVKMPENRD
HHCCCCCCCCCCCCCHHHHHHHHHHHHHHEEEEEHHHHCCCCCHHHHHHHHHHHCCCCCC
IRCFVIGGNCLGCIYRVPSEGEWRSNVALGGKVRALKDSTEPCELAVKAAEALKGEVVSI
EEEEEECCCEEEEEEECCCCCCCCCCEEECCEEEEECCCCCHHHHHHHHHHHHCCCEEEE
DIFEGKEGYLVNEVNGVPEFKGFMKATGINVGDEIAKYVYERVKR
EEEECCCCEEEECCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Glu; Ribosomal protein S6 [C]

Specific reaction: Catalyzes the addition of glutamic acids to the carboxyl end of ribosomal protein S6 [C]

General reaction: Ligases; Forming Carbon-Nitrogen Bonds; Acid-D-Amino-Acid Ligases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12963379 [H]