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The map label for this gene is nadE [H]

Identifier: 156938178

GI number: 156938178

Start: 1252125

End: 1252952

Strand: Reverse

Name: nadE [H]

Synonym: Igni_1391

Alternate gene names: 156938178

Gene position: 1252952-1252125 (Counterclockwise)

Preceding gene: 156938179

Following gene: 156938176

Centisome position: 96.56

GC content: 55.8

Gene sequence:

>828_bases
ATGAGAATGAAACTGCCGGAGCCGGACTGGGACTCTGTTAGGAAGAGGATAGTAGGGTGGCTGAAGGATTTCTTCCAGCG
AACCGGGAGGGTGGCCGTAATAGGGCTTAGCGGCGGGGTTGATTCCGCGGTTACTGCGGCGTTACTGAGCGAGGCTTTAG
GCCCCGAGAGGGTGCGAGCGCTTATCTTGCCCTCCGCCTCTACACCAGAGAAAGACGTGGAAGACGCCAAGCGCGTCGCC
CGGCTCCTCGGCATCGAGGACGTGAGAGTGATTGACGTAGAGCCGGCCCTCCGAGCTTTCGACGACCTACTAAAGGTCGA
CGACAGGGTCGTGAGAGGCAACACCCTAGCAAGGATAAGGATGACCATACTCTACGCCGTAGCTCACAAGGACGGCATAG
TCGTAGGCACCGGGGACAAGAGCGAGTTCTTGTTGGGCTACTTCACCAAGTGCGGCGACGGCTGTGCGGACGTCTTCCCC
ATAGGGGACCTTTACAAGACCTGGGTAAGGAAGCTCGCGTTGCACATGGGCTTGCCGGAGGACGTGGCATTGAAGCCTTC
GAGCCCCCGCCTCTGGCCCGGCCATTTGGCTGAGGAGGAGTTGGGCTTTAGTTACGAGGTAGCCGACGTGGTCCTCTACA
ATCTCGTTGAGAACGGTATGAGGCCCCAAGAAATAGTGGAGAGGTTAGGAGTAGACAAAGAAATCGTGGACAGAATAATC
CAAAGGATAAAGTTAAATAAGCATAAACTCATAACTCCTCCAATCGTCAAAGTAACGAGAAGGACAGCCTTCGAGTTCGT
CGAGGAGGCGGATGCGCTGCTAACTTAA

Upstream 100 bases:

>100_bases
GCAGATGCAAGTAGAAGACCTCGAAAAACTCTTACAAGAGTATAGGGCCTCCCGTTGGAAGTGAGCTTTATATTAAGGAC
CAAGTTACAAAGGCTCGGTT

Downstream 100 bases:

>100_bases
TAAGGGGAAGCCGCTGAGCTCACAACGCCGGGGTGCCCGAGCGGCCCAAGGGGGTGGGCTCGAGACCTCCAGCGCCCGAA
GCGACCGAGAGACCCACTGC

Product: NH(3)-dependent NAD(+) synthetase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 275; Mature: 275

Protein sequence:

>275_residues
MRMKLPEPDWDSVRKRIVGWLKDFFQRTGRVAVIGLSGGVDSAVTAALLSEALGPERVRALILPSASTPEKDVEDAKRVA
RLLGIEDVRVIDVEPALRAFDDLLKVDDRVVRGNTLARIRMTILYAVAHKDGIVVGTGDKSEFLLGYFTKCGDGCADVFP
IGDLYKTWVRKLALHMGLPEDVALKPSSPRLWPGHLAEEELGFSYEVADVVLYNLVENGMRPQEIVERLGVDKEIVDRII
QRIKLNKHKLITPPIVKVTRRTAFEFVEEADALLT

Sequences:

>Translated_275_residues
MRMKLPEPDWDSVRKRIVGWLKDFFQRTGRVAVIGLSGGVDSAVTAALLSEALGPERVRALILPSASTPEKDVEDAKRVA
RLLGIEDVRVIDVEPALRAFDDLLKVDDRVVRGNTLARIRMTILYAVAHKDGIVVGTGDKSEFLLGYFTKCGDGCADVFP
IGDLYKTWVRKLALHMGLPEDVALKPSSPRLWPGHLAEEELGFSYEVADVVLYNLVENGMRPQEIVERLGVDKEIVDRII
QRIKLNKHKLITPPIVKVTRRTAFEFVEEADALLT
>Mature_275_residues
MRMKLPEPDWDSVRKRIVGWLKDFFQRTGRVAVIGLSGGVDSAVTAALLSEALGPERVRALILPSASTPEKDVEDAKRVA
RLLGIEDVRVIDVEPALRAFDDLLKVDDRVVRGNTLARIRMTILYAVAHKDGIVVGTGDKSEFLLGYFTKCGDGCADVFP
IGDLYKTWVRKLALHMGLPEDVALKPSSPRLWPGHLAEEELGFSYEVADVVLYNLVENGMRPQEIVERLGVDKEIVDRII
QRIKLNKHKLITPPIVKVTRRTAFEFVEEADALLT

Specific function: This NAD Synthase Uses Nh(3) In Preference To Glutamine. [C]

COG id: COG0171

COG function: function code H; NAD synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1788036, Length=239, Percent_Identity=27.1966527196653, Blast_Score=72, Evalue=5e-14,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR022926
- InterPro:   IPR014729 [H]

Pfam domain/function: PF02540 NAD_synthase [H]

EC number: =6.3.1.5 [H]

Molecular weight: Translated: 30694; Mature: 30694

Theoretical pI: Translated: 6.54; Mature: 6.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRMKLPEPDWDSVRKRIVGWLKDFFQRTGRVAVIGLSGGVDSAVTAALLSEALGPERVRA
CCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHCCHHHHHE
LILPSASTPEKDVEDAKRVARLLGIEDVRVIDVEPALRAFDDLLKVDDRVVRGNTLARIR
EEECCCCCCHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHCCHHHCCCHHHHHH
MTILYAVAHKDGIVVGTGDKSEFLLGYFTKCGDGCADVFPIGDLYKTWVRKLALHMGLPE
HHHHHHHHCCCCEEEECCCCCHHHHHHHHHCCCCHHHHCCCHHHHHHHHHHHHHHCCCCC
DVALKPSSPRLWPGHLAEEELGFSYEVADVVLYNLVENGMRPQEIVERLGVDKEIVDRII
CCEECCCCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHH
QRIKLNKHKLITPPIVKVTRRTAFEFVEEADALLT
HHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRMKLPEPDWDSVRKRIVGWLKDFFQRTGRVAVIGLSGGVDSAVTAALLSEALGPERVRA
CCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHCCHHHHHE
LILPSASTPEKDVEDAKRVARLLGIEDVRVIDVEPALRAFDDLLKVDDRVVRGNTLARIR
EEECCCCCCHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHCCHHHCCCHHHHHH
MTILYAVAHKDGIVVGTGDKSEFLLGYFTKCGDGCADVFPIGDLYKTWVRKLALHMGLPE
HHHHHHHHCCCCEEEECCCCCHHHHHHHHHCCCCHHHHCCCHHHHHHHHHHHHHHCCCCC
DVALKPSSPRLWPGHLAEEELGFSYEVADVVLYNLVENGMRPQEIVERLGVDKEIVDRII
CCEECCCCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHH
QRIKLNKHKLITPPIVKVTRRTAFEFVEEADALLT
HHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA