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The map label for this gene is metG

Identifier: 156937943

GI number: 156937943

Start: 1031812

End: 1033497

Strand: Reverse

Name: metG

Synonym: Igni_1155

Alternate gene names: 156937943

Gene position: 1033497-1031812 (Counterclockwise)

Preceding gene: 156937945

Following gene: 156937942

Centisome position: 79.65

GC content: 58.36

Gene sequence:

>1686_bases
GTGGCCAAGTGGATAGTCGGGAGCGCGTGGCCCTACGTCAACACCGTGCCTCACTTAGGTAACTTGATCGGGTCCGTACT
GTCCGCCGACGTGTTCGCTAGGTTCTTGAGGCTTATGGGAGAGGACGTAGTGTTCGTCTCCGGCAGCGACGAGCACGGGA
CCCCCATAGAGGTGGAGGCCCGGAAGAGGGGGGTGGAGCCCAAGGAGCTGACCGACAAGGTGCACGAGTACGTAAAGAAG
CTGTTCGAGAAGTACTTGATATCCTTCGACAATTACACGCGCACCCACAACCCGGTGCACATGGAGTTCGTGAGGGAAAC
GTTTATGAAGATATACGAGAACGGCTACATATTCACGCAAGAGATGGTCATGCCCTACTGCCCCAAGGACAAGATGTTCT
TGCCCGACAGGTTTACGGTGGGCACTTGCCCCTACTGCGGGGCCCCGGACGCCAGGGGGGACCAGTGCGAGAGGTGTGGA
AAGCTCTTGGACCCCCCGGACTTGGTCAACCCCCGCTGCGCCTTCTGCGGCTCCAGGCCGGTCTGGAGGAAGACCTTGCA
CTGGTTCTTCGACTTGCCTAAGGCCGCGGAGGGGTTGGTGGAGTGGCTGGAAAGGAGCGAGCTGCCCAACAACGTTAAGA
AGTTCACCTTGAACTGGGTGAAGGAGGGCCTCACGCCGAGGTCTGTCACTAGGGACAACAAGTGGGGCATACCCGCCCCG
TTCCCGGGGGCGGAGGGCAAGACCATCTACGTTTGGTTCGAAGCGGTCTTGGGTTACCTCTCCGCAGTTAAGGAGTTGGA
CGTCAAGAACGGGACGAACTTGTTCGAGGAGTTCTGGAAGGACATCAACTCCAGGCCGGTATACTTCATAGGGAAGGACA
ACATACCCTTCCACTCCATAATACTCCCGGCCCTCTTAAAGGCGACCGGCGAGGAGTACCCGCTCCCCTACAACATATCC
GCGACGGAGTACCTCATGTACGAGGGCCAGAAGTTCAGCAAGAGGAGGAGGGTGGGCGTTTGGATAGACGAGGCGCTGGA
GGTGGTGCCCAACCCGGACTACTGGAGGTTCGCGTTGATTAGAATGAGGCCCGAGGAGAGGGACACAAACTTCACTTGGA
GGGAGTTCTACAGGATAGTGAACAGCGAGCTGAACGACGACATAGGCAACTTCGCCCACAGGGTGTTAACTTTCGTGGAG
AGGCGCTTCGGAGGCTCGGTCCGCGGGAGGGTGGACGAGGAGGTCAAGAAGAGCATAGCCGAGTTGCACGAGAAGTACGT
GAAGGCCATGTATAAGGTGAAGTTGAAGGAAGCGTCCGGCTTAGTCTTGGAAATGGCCAGGTTAGGTAACAAGTACTTGA
ACGAGAAGGAGCCTTGGAGGCTCTTGAAGGAGGGGAAGGAGGAAGAGGCGAGGGACGTCATGTATACGTGCTTGTTCATA
CTTAGGGAGGTCGCCCTTCACTTGGCGCCCTTCGCGCCCTCGGCGGCGGAGGAGCTGTGGAAGATGATAGGCGAGCCCGG
GAGCGTGCACGAGAGGGGCAAGCTCCTCACCAGCGGTTCCGAGCCCGCGGGGGAGGTCAAAGAGCCCAAGCCGCTGTTCC
AGAAGCTGCCCAAGGACTTCCTCGAGAGGGTGGACGAGCTGTTGGAGGAGGCTCGGAGAAAAGTTGAAAAGCTCCGCCCC
ATCTGA

Upstream 100 bases:

>100_bases
AGGGACCTATTGCGGTATCATCCGCCCCCCAAGCTTTAAACCCTCGCCTACACAGCTCCCGTTTTATGCTCGCGCGGCCC
AACGCCCCCGGGGGCCGACG

Downstream 100 bases:

>100_bases
CTAACTGGTAAGGAAACAGTTAATAATGGCGTGCTCTTAGTTAGCGTGAGGAACTCCCATGCCAGAGACCCGTGCCCTGC
ACGAGGGCAACAAGAACTCT

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS

Number of amino acids: Translated: 561; Mature: 560

Protein sequence:

>561_residues
MAKWIVGSAWPYVNTVPHLGNLIGSVLSADVFARFLRLMGEDVVFVSGSDEHGTPIEVEARKRGVEPKELTDKVHEYVKK
LFEKYLISFDNYTRTHNPVHMEFVRETFMKIYENGYIFTQEMVMPYCPKDKMFLPDRFTVGTCPYCGAPDARGDQCERCG
KLLDPPDLVNPRCAFCGSRPVWRKTLHWFFDLPKAAEGLVEWLERSELPNNVKKFTLNWVKEGLTPRSVTRDNKWGIPAP
FPGAEGKTIYVWFEAVLGYLSAVKELDVKNGTNLFEEFWKDINSRPVYFIGKDNIPFHSIILPALLKATGEEYPLPYNIS
ATEYLMYEGQKFSKRRRVGVWIDEALEVVPNPDYWRFALIRMRPEERDTNFTWREFYRIVNSELNDDIGNFAHRVLTFVE
RRFGGSVRGRVDEEVKKSIAELHEKYVKAMYKVKLKEASGLVLEMARLGNKYLNEKEPWRLLKEGKEEEARDVMYTCLFI
LREVALHLAPFAPSAAEELWKMIGEPGSVHERGKLLTSGSEPAGEVKEPKPLFQKLPKDFLERVDELLEEARRKVEKLRP
I

Sequences:

>Translated_561_residues
MAKWIVGSAWPYVNTVPHLGNLIGSVLSADVFARFLRLMGEDVVFVSGSDEHGTPIEVEARKRGVEPKELTDKVHEYVKK
LFEKYLISFDNYTRTHNPVHMEFVRETFMKIYENGYIFTQEMVMPYCPKDKMFLPDRFTVGTCPYCGAPDARGDQCERCG
KLLDPPDLVNPRCAFCGSRPVWRKTLHWFFDLPKAAEGLVEWLERSELPNNVKKFTLNWVKEGLTPRSVTRDNKWGIPAP
FPGAEGKTIYVWFEAVLGYLSAVKELDVKNGTNLFEEFWKDINSRPVYFIGKDNIPFHSIILPALLKATGEEYPLPYNIS
ATEYLMYEGQKFSKRRRVGVWIDEALEVVPNPDYWRFALIRMRPEERDTNFTWREFYRIVNSELNDDIGNFAHRVLTFVE
RRFGGSVRGRVDEEVKKSIAELHEKYVKAMYKVKLKEASGLVLEMARLGNKYLNEKEPWRLLKEGKEEEARDVMYTCLFI
LREVALHLAPFAPSAAEELWKMIGEPGSVHERGKLLTSGSEPAGEVKEPKPLFQKLPKDFLERVDELLEEARRKVEKLRP
I
>Mature_560_residues
AKWIVGSAWPYVNTVPHLGNLIGSVLSADVFARFLRLMGEDVVFVSGSDEHGTPIEVEARKRGVEPKELTDKVHEYVKKL
FEKYLISFDNYTRTHNPVHMEFVRETFMKIYENGYIFTQEMVMPYCPKDKMFLPDRFTVGTCPYCGAPDARGDQCERCGK
LLDPPDLVNPRCAFCGSRPVWRKTLHWFFDLPKAAEGLVEWLERSELPNNVKKFTLNWVKEGLTPRSVTRDNKWGIPAPF
PGAEGKTIYVWFEAVLGYLSAVKELDVKNGTNLFEEFWKDINSRPVYFIGKDNIPFHSIILPALLKATGEEYPLPYNISA
TEYLMYEGQKFSKRRRVGVWIDEALEVVPNPDYWRFALIRMRPEERDTNFTWREFYRIVNSELNDDIGNFAHRVLTFVER
RFGGSVRGRVDEEVKKSIAELHEKYVKAMYKVKLKEASGLVLEMARLGNKYLNEKEPWRLLKEGKEEEARDVMYTCLFIL
REVALHLAPFAPSAAEELWKMIGEPGSVHERGKLLTSGSEPAGEVKEPKPLFQKLPKDFLERVDELLEEARRKVEKLRPI

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 1 subfamily

Homologues:

Organism=Homo sapiens, GI14043022, Length=556, Percent_Identity=37.7697841726619, Blast_Score=385, Evalue=1e-107,
Organism=Homo sapiens, GI24308436, Length=535, Percent_Identity=26.7289719626168, Blast_Score=155, Evalue=8e-38,
Organism=Escherichia coli, GI1788432, Length=503, Percent_Identity=31.4115308151093, Blast_Score=276, Evalue=2e-75,
Organism=Caenorhabditis elegans, GI17540794, Length=547, Percent_Identity=38.0255941499086, Blast_Score=390, Evalue=1e-108,
Organism=Caenorhabditis elegans, GI212645096, Length=517, Percent_Identity=26.4990328820116, Blast_Score=136, Evalue=3e-32,
Organism=Caenorhabditis elegans, GI71992781, Length=418, Percent_Identity=24.4019138755981, Blast_Score=83, Evalue=4e-16,
Organism=Saccharomyces cerevisiae, GI6321704, Length=514, Percent_Identity=37.3540856031128, Blast_Score=370, Evalue=1e-103,
Organism=Saccharomyces cerevisiae, GI6321610, Length=349, Percent_Identity=27.2206303724928, Blast_Score=113, Evalue=7e-26,
Organism=Drosophila melanogaster, GI24655311, Length=554, Percent_Identity=38.9891696750903, Blast_Score=410, Evalue=1e-114,
Organism=Drosophila melanogaster, GI21358483, Length=581, Percent_Identity=24.4406196213425, Blast_Score=137, Evalue=2e-32,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): SYM_IGNH4 (A8ABN0)

Other databases:

- EMBL:   CP000816
- RefSeq:   YP_001435739.1
- STRING:   A8ABN0
- GeneID:   5563016
- GenomeReviews:   CP000816_GR
- KEGG:   iho:Igni_1155
- eggNOG:   arNOG04371
- HOGENOM:   HBG721667
- OMA:   WGIWIDE
- ProtClustDB:   PRK12268
- BioCyc:   IHOS453591:IGNI_1155-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00098
- InterPro:   IPR015413
- InterPro:   IPR014758
- InterPro:   IPR014729
- InterPro:   IPR009080
- InterPro:   IPR013155
- Gene3D:   G3DSA:3.40.50.620
- PRINTS:   PR01041
- TIGRFAMs:   TIGR00398

Pfam domain/function: PF08264 Anticodon_1; PF09334 tRNA-synt_1g; SSF47323 tRNAsyn_1a_bind

EC number: =6.1.1.10

Molecular weight: Translated: 65012; Mature: 64880

Theoretical pI: Translated: 7.30; Mature: 7.30

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 334-334

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKWIVGSAWPYVNTVPHLGNLIGSVLSADVFARFLRLMGEDVVFVSGSDEHGTPIEVEA
CCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEHH
RKRGVEPKELTDKVHEYVKKLFEKYLISFDNYTRTHNPVHMEFVRETFMKIYENGYIFTQ
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEC
EMVMPYCPKDKMFLPDRFTVGTCPYCGAPDARGDQCERCGKLLDPPDLVNPRCAFCGSRP
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCHHHCCCCH
VWRKTLHWFFDLPKAAEGLVEWLERSELPNNVKKFTLNWVKEGLTPRSVTRDNKWGIPAP
HHHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
FPGAEGKTIYVWFEAVLGYLSAVKELDVKNGTNLFEEFWKDINSRPVYFIGKDNIPFHSI
CCCCCCCEEEEEHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCEEEEECCCCCHHHH
ILPALLKATGEEYPLPYNISATEYLMYEGQKFSKRRRVGVWIDEALEVVPNPDYWRFALI
HHHHHHHHCCCCCCCCCCCCHHHHHHHCCHHHHHHCCCCHHHHHHHHHCCCCCHHEEEEE
RMRPEERDTNFTWREFYRIVNSELNDDIGNFAHRVLTFVERRFGGSVRGRVDEEVKKSIA
EECCCCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHH
ELHEKYVKAMYKVKLKEASGLVLEMARLGNKYLNEKEPWRLLKEGKEEEARDVMYTCLFI
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCHHHHHHCCCHHHHHHHHHHHHHH
LREVALHLAPFAPSAAEELWKMIGEPGSVHERGKLLTSGSEPAGEVKEPKPLFQKLPKDF
HHHHHHHHCCCCCHHHHHHHHHHCCCCCCHHCCCEECCCCCCCCCCCCCCHHHHHCCHHH
LERVDELLEEARRKVEKLRPI
HHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AKWIVGSAWPYVNTVPHLGNLIGSVLSADVFARFLRLMGEDVVFVSGSDEHGTPIEVEA
CCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEHH
RKRGVEPKELTDKVHEYVKKLFEKYLISFDNYTRTHNPVHMEFVRETFMKIYENGYIFTQ
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEC
EMVMPYCPKDKMFLPDRFTVGTCPYCGAPDARGDQCERCGKLLDPPDLVNPRCAFCGSRP
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCHHHCCCCH
VWRKTLHWFFDLPKAAEGLVEWLERSELPNNVKKFTLNWVKEGLTPRSVTRDNKWGIPAP
HHHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
FPGAEGKTIYVWFEAVLGYLSAVKELDVKNGTNLFEEFWKDINSRPVYFIGKDNIPFHSI
CCCCCCCEEEEEHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCEEEEECCCCCHHHH
ILPALLKATGEEYPLPYNISATEYLMYEGQKFSKRRRVGVWIDEALEVVPNPDYWRFALI
HHHHHHHHCCCCCCCCCCCCHHHHHHHCCHHHHHHCCCCHHHHHHHHHCCCCCHHEEEEE
RMRPEERDTNFTWREFYRIVNSELNDDIGNFAHRVLTFVERRFGGSVRGRVDEEVKKSIA
EECCCCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHH
ELHEKYVKAMYKVKLKEASGLVLEMARLGNKYLNEKEPWRLLKEGKEEEARDVMYTCLFI
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCHHHHHHCCCHHHHHHHHHHHHHH
LREVALHLAPFAPSAAEELWKMIGEPGSVHERGKLLTSGSEPAGEVKEPKPLFQKLPKDF
HHHHHHHHCCCCCHHHHHHHHHHCCCCCCHHCCCEECCCCCCCCCCCCCCHHHHHCCHHH
LERVDELLEEARRKVEKLRPI
HHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA