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The map label for this gene is tuf

Identifier: 156937938

GI number: 156937938

Start: 1026397

End: 1027725

Strand: Reverse

Name: tuf

Synonym: Igni_1150

Alternate gene names: 156937938

Gene position: 1027725-1026397 (Counterclockwise)

Preceding gene: 156937939

Following gene: 156937931

Centisome position: 79.21

GC content: 58.92

Gene sequence:

>1329_bases
ATGAGCAAGAAAGAGAAGCCCCACATGAACTTGATAGTGATCGGCCACGTAGACCACGGCAAGAGCACCCTAGTGGGCCA
CCTGTTGTACGAGCTGGGCTTCGTCGACGAGAAGACGCTGAAGATGTTGGAGGAGGAAGCGAAGAAGAGGGGCAAGGAGA
GCTTCAAGTACGCTTGGCTGCTGGACAAGCTCAAGGAGGAGCGTGAGAGGGGCGTTACCATCGACCTGACCTTCATGAAG
TTCGAGACTCCGAAGTACTACTTCACCATCATCGACGCCCCCGGCCACAGGGACTTCATTAAGAACATGATCACCGGCGC
CAGCCAGGCCGACGCGGCCATCTTGGTGGTCAGCGCCAGGCCGGGCGAGTTCGAGGCCGGCATGAGCGCGGAGGGCCAGA
CTAGGGAACACATACTCCTAGCCAAGACCATGGGTATCGACCAGATAATAGTGGCCGTCAACAAGATGGACGCCACCGAG
CCGCCTTGGAGCGAGAAGAGGTACAAGCAGATAGTGGAGACCCTGAAGAAGTTCATGAAGGGCCTCGGCTTCAAGGTGGA
CGAGATACCGTTCGTGCCCGTTAGCGCTTGGACCGGCGACAACATCATCAAGAGGAGCGAGAACATGCCGTGGTACAAGG
GCCCCACCTTGGTGGAGGCCTTGGACAACCTCAAGCCGCCAAGCGTGGAGAAGTGGGCCAAGCTCCCGCTTAGGATACCC
ATCCAAGACGTCTACAGCATCACCGGCGTGGGCACCGTCCCGGTAGGGAGGGTGGAGACCGGCGTACTCAAGGTAGGCGA
CAAGGTAGTGTTCATGCCGCCCGGAGTTGGAGGAGAAGTAAGGAGCATAGAGATGCACCACGAGAAGATCGAGCAAGCCA
TGCCCGGCGACAACATAGGCTTCAACGTAAGGGGCGTGAGCAAGAACGACATAAAGAGGGGAGACGTGGCCGGCCACCCG
GAGAACCCGCCCACCGTTGCGGACCAGTTCACCGCCAGGGTGTTCGTCATCTGGCACCCCAGCGCCATAGCGGTGGGCTA
CACCCCGGTGATCCACGCCCACACCGCCAGCGTGGCTAGCAGGATTATCGAGATCAAGCAGAAGATCGACCCGAGGACCG
GTAAGGTGATCGAGGAGAACCCGAGCTTCCTCAAGCCGGGCGACGCCGCTGTGGTGGTGTTCAAGCCGCTCAAGCCGATG
GTGATAGAGAAGTTCCAAGAGTTCCAGCCGCTGGGCAGGTTCGCCATGAGGGACATGGGCAAGACCGTCGGCATAGGCAT
AGTGACCGACGTCAAGCCCGCCAAAGTAGAGATAAAGATGAAGAAGTAA

Upstream 100 bases:

>100_bases
CGAACCAGCTCACCCTCACCTCATCGGCCTTCGAGTGCTACCATGGTACCAATCTTTTTAAGTCGACACCGACTAGCCCT
CGGACGGAGGGATCAGTAGT

Downstream 100 bases:

>100_bases
GCCCTTCAGTTTTTGTCCCCTTCTCTTATTAATTCCTTCACTATCACGTGTATACACGGCAAGGTCAAGAGGACCCCCGT
GGGTAACAAGAAGGCCCTCT

Product: elongation factor 1-alpha

Products: GDP; phosphate

Alternate protein names: EF-1-alpha; Elongation factor Tu; EF-Tu

Number of amino acids: Translated: 442; Mature: 441

Protein sequence:

>442_residues
MSKKEKPHMNLIVIGHVDHGKSTLVGHLLYELGFVDEKTLKMLEEEAKKRGKESFKYAWLLDKLKEERERGVTIDLTFMK
FETPKYYFTIIDAPGHRDFIKNMITGASQADAAILVVSARPGEFEAGMSAEGQTREHILLAKTMGIDQIIVAVNKMDATE
PPWSEKRYKQIVETLKKFMKGLGFKVDEIPFVPVSAWTGDNIIKRSENMPWYKGPTLVEALDNLKPPSVEKWAKLPLRIP
IQDVYSITGVGTVPVGRVETGVLKVGDKVVFMPPGVGGEVRSIEMHHEKIEQAMPGDNIGFNVRGVSKNDIKRGDVAGHP
ENPPTVADQFTARVFVIWHPSAIAVGYTPVIHAHTASVASRIIEIKQKIDPRTGKVIEENPSFLKPGDAAVVVFKPLKPM
VIEKFQEFQPLGRFAMRDMGKTVGIGIVTDVKPAKVEIKMKK

Sequences:

>Translated_442_residues
MSKKEKPHMNLIVIGHVDHGKSTLVGHLLYELGFVDEKTLKMLEEEAKKRGKESFKYAWLLDKLKEERERGVTIDLTFMK
FETPKYYFTIIDAPGHRDFIKNMITGASQADAAILVVSARPGEFEAGMSAEGQTREHILLAKTMGIDQIIVAVNKMDATE
PPWSEKRYKQIVETLKKFMKGLGFKVDEIPFVPVSAWTGDNIIKRSENMPWYKGPTLVEALDNLKPPSVEKWAKLPLRIP
IQDVYSITGVGTVPVGRVETGVLKVGDKVVFMPPGVGGEVRSIEMHHEKIEQAMPGDNIGFNVRGVSKNDIKRGDVAGHP
ENPPTVADQFTARVFVIWHPSAIAVGYTPVIHAHTASVASRIIEIKQKIDPRTGKVIEENPSFLKPGDAAVVVFKPLKPM
VIEKFQEFQPLGRFAMRDMGKTVGIGIVTDVKPAKVEIKMKK
>Mature_441_residues
SKKEKPHMNLIVIGHVDHGKSTLVGHLLYELGFVDEKTLKMLEEEAKKRGKESFKYAWLLDKLKEERERGVTIDLTFMKF
ETPKYYFTIIDAPGHRDFIKNMITGASQADAAILVVSARPGEFEAGMSAEGQTREHILLAKTMGIDQIIVAVNKMDATEP
PWSEKRYKQIVETLKKFMKGLGFKVDEIPFVPVSAWTGDNIIKRSENMPWYKGPTLVEALDNLKPPSVEKWAKLPLRIPI
QDVYSITGVGTVPVGRVETGVLKVGDKVVFMPPGVGGEVRSIEMHHEKIEQAMPGDNIGFNVRGVSKNDIKRGDVAGHPE
NPPTVADQFTARVFVIWHPSAIAVGYTPVIHAHTASVASRIIEIKQKIDPRTGKVIEENPSFLKPGDAAVVVFKPLKPMV
IEKFQEFQPLGRFAMRDMGKTVGIGIVTDVKPAKVEIKMKK

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis

COG id: COG5256

COG function: function code J; Translation elongation factor EF-1alpha (GTPase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily

Homologues:

Organism=Homo sapiens, GI4503471, Length=441, Percent_Identity=56.9160997732426, Blast_Score=530, Evalue=1e-151,
Organism=Homo sapiens, GI4503475, Length=442, Percent_Identity=55.8823529411765, Blast_Score=525, Evalue=1e-149,
Organism=Homo sapiens, GI223555963, Length=432, Percent_Identity=41.6666666666667, Blast_Score=359, Evalue=2e-99,
Organism=Homo sapiens, GI5729864, Length=432, Percent_Identity=41.6666666666667, Blast_Score=358, Evalue=4e-99,
Organism=Homo sapiens, GI194018520, Length=431, Percent_Identity=39.4431554524362, Blast_Score=324, Evalue=1e-88,
Organism=Homo sapiens, GI194097354, Length=431, Percent_Identity=39.4431554524362, Blast_Score=324, Evalue=1e-88,
Organism=Homo sapiens, GI194018522, Length=431, Percent_Identity=39.4431554524362, Blast_Score=323, Evalue=1e-88,
Organism=Homo sapiens, GI46094014, Length=431, Percent_Identity=38.9791183294664, Blast_Score=319, Evalue=3e-87,
Organism=Homo sapiens, GI34147630, Length=435, Percent_Identity=31.4942528735632, Blast_Score=193, Evalue=3e-49,
Organism=Homo sapiens, GI169162126, Length=140, Percent_Identity=40.7142857142857, Blast_Score=108, Evalue=8e-24,
Organism=Homo sapiens, GI169162824, Length=140, Percent_Identity=40.7142857142857, Blast_Score=108, Evalue=8e-24,
Organism=Homo sapiens, GI239752724, Length=140, Percent_Identity=40.7142857142857, Blast_Score=108, Evalue=9e-24,
Organism=Homo sapiens, GI82546879, Length=446, Percent_Identity=24.6636771300448, Blast_Score=101, Evalue=1e-21,
Organism=Homo sapiens, GI54607086, Length=346, Percent_Identity=26.0115606936416, Blast_Score=92, Evalue=8e-19,
Organism=Homo sapiens, GI45593140, Length=463, Percent_Identity=23.9740820734341, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI157426893, Length=267, Percent_Identity=27.3408239700375, Blast_Score=75, Evalue=1e-13,
Organism=Escherichia coli, GI1789108, Length=439, Percent_Identity=33.0296127562642, Blast_Score=199, Evalue=3e-52,
Organism=Escherichia coli, GI1789737, Length=435, Percent_Identity=31.264367816092, Blast_Score=180, Evalue=1e-46,
Organism=Escherichia coli, GI1790412, Length=435, Percent_Identity=31.264367816092, Blast_Score=180, Evalue=1e-46,
Organism=Escherichia coli, GI2367247, Length=389, Percent_Identity=25.706940874036, Blast_Score=102, Evalue=6e-23,
Organism=Escherichia coli, GI48994988, Length=265, Percent_Identity=28.6792452830189, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1788922, Length=313, Percent_Identity=24.9201277955272, Blast_Score=74, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17552884, Length=442, Percent_Identity=54.7511312217195, Blast_Score=513, Evalue=1e-146,
Organism=Caenorhabditis elegans, GI17569207, Length=442, Percent_Identity=54.7511312217195, Blast_Score=513, Evalue=1e-146,
Organism=Caenorhabditis elegans, GI32566303, Length=442, Percent_Identity=50, Blast_Score=449, Evalue=1e-126,
Organism=Caenorhabditis elegans, GI32566629, Length=430, Percent_Identity=40.2325581395349, Blast_Score=315, Evalue=2e-86,
Organism=Caenorhabditis elegans, GI115532067, Length=434, Percent_Identity=39.8617511520737, Blast_Score=297, Evalue=7e-81,
Organism=Caenorhabditis elegans, GI115532065, Length=434, Percent_Identity=39.8617511520737, Blast_Score=296, Evalue=2e-80,
Organism=Caenorhabditis elegans, GI32566301, Length=168, Percent_Identity=61.9047619047619, Blast_Score=219, Evalue=3e-57,
Organism=Caenorhabditis elegans, GI17556456, Length=443, Percent_Identity=33.4085778781038, Blast_Score=187, Evalue=8e-48,
Organism=Caenorhabditis elegans, GI25141371, Length=362, Percent_Identity=32.3204419889503, Blast_Score=161, Evalue=6e-40,
Organism=Caenorhabditis elegans, GI32566299, Length=62, Percent_Identity=61.2903225806452, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17565438, Length=450, Percent_Identity=21.5555555555556, Blast_Score=85, Evalue=7e-17,
Organism=Caenorhabditis elegans, GI17506081, Length=305, Percent_Identity=26.2295081967213, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17509919, Length=349, Percent_Identity=22.9226361031519, Blast_Score=71, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17557151, Length=260, Percent_Identity=28.0769230769231, Blast_Score=71, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6325337, Length=441, Percent_Identity=55.5555555555556, Blast_Score=509, Evalue=1e-145,
Organism=Saccharomyces cerevisiae, GI6319594, Length=441, Percent_Identity=55.5555555555556, Blast_Score=509, Evalue=1e-145,
Organism=Saccharomyces cerevisiae, GI6320377, Length=430, Percent_Identity=38.1395348837209, Blast_Score=306, Evalue=4e-84,
Organism=Saccharomyces cerevisiae, GI6322937, Length=357, Percent_Identity=36.1344537815126, Blast_Score=238, Evalue=2e-63,
Organism=Saccharomyces cerevisiae, GI6324761, Length=437, Percent_Identity=32.9519450800915, Blast_Score=202, Evalue=8e-53,
Organism=Saccharomyces cerevisiae, GI6323320, Length=282, Percent_Identity=28.3687943262411, Blast_Score=71, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6324650, Length=247, Percent_Identity=25.5060728744939, Blast_Score=71, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24652838, Length=441, Percent_Identity=56.9160997732426, Blast_Score=526, Evalue=1e-149,
Organism=Drosophila melanogaster, GI17137572, Length=441, Percent_Identity=56.9160997732426, Blast_Score=526, Evalue=1e-149,
Organism=Drosophila melanogaster, GI45553807, Length=441, Percent_Identity=56.4625850340136, Blast_Score=517, Evalue=1e-147,
Organism=Drosophila melanogaster, GI45553816, Length=441, Percent_Identity=56.4625850340136, Blast_Score=517, Evalue=1e-147,
Organism=Drosophila melanogaster, GI24651721, Length=441, Percent_Identity=56.4625850340136, Blast_Score=517, Evalue=1e-147,
Organism=Drosophila melanogaster, GI17864154, Length=441, Percent_Identity=56.4625850340136, Blast_Score=517, Evalue=1e-147,
Organism=Drosophila melanogaster, GI45550900, Length=431, Percent_Identity=38.7470997679814, Blast_Score=332, Evalue=3e-91,
Organism=Drosophila melanogaster, GI17137380, Length=433, Percent_Identity=35.796766743649, Blast_Score=297, Evalue=1e-80,
Organism=Drosophila melanogaster, GI281363316, Length=434, Percent_Identity=33.8709677419355, Blast_Score=213, Evalue=3e-55,
Organism=Drosophila melanogaster, GI17864358, Length=434, Percent_Identity=33.8709677419355, Blast_Score=213, Evalue=3e-55,
Organism=Drosophila melanogaster, GI19921738, Length=436, Percent_Identity=27.2935779816514, Blast_Score=160, Evalue=1e-39,
Organism=Drosophila melanogaster, GI19922690, Length=316, Percent_Identity=25.9493670886076, Blast_Score=92, Evalue=6e-19,
Organism=Drosophila melanogaster, GI19922512, Length=444, Percent_Identity=23.6486486486486, Blast_Score=89, Evalue=4e-18,
Organism=Drosophila melanogaster, GI24654852, Length=444, Percent_Identity=23.6486486486486, Blast_Score=89, Evalue=4e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): EF1A_IGNH4 (A8ABM5)

Other databases:

- EMBL:   CP000816
- RefSeq:   YP_001435734.1
- ProteinModelPortal:   A8ABM5
- SMR:   A8ABM5
- STRING:   A8ABM5
- GeneID:   5561918
- GenomeReviews:   CP000816_GR
- KEGG:   iho:Igni_1150
- eggNOG:   arNOG04484
- HOGENOM:   HBG307581
- OMA:   AFIGHVD
- ProtClustDB:   PRK12317
- BioCyc:   IHOS453591:IGNI_1150-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00118_A
- InterPro:   IPR000795
- InterPro:   IPR009001
- InterPro:   IPR004539
- InterPro:   IPR004161
- InterPro:   IPR004160
- InterPro:   IPR009000
- PRINTS:   PR00315
- TIGRFAMs:   TIGR00483

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3; SSF50465 Elong_init_C; SSF50447 Translat_factor

EC number: 3.6.5.3

Molecular weight: Translated: 49265; Mature: 49134

Theoretical pI: Translated: 9.81; Mature: 9.81

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKKEKPHMNLIVIGHVDHGKSTLVGHLLYELGFVDEKTLKMLEEEAKKRGKESFKYAWL
CCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHH
LDKLKEERERGVTIDLTFMKFETPKYYFTIIDAPGHRDFIKNMITGASQADAAILVVSAR
HHHHHHHHHCCCEEEEEEEEEECCEEEEEEEECCCCHHHHHHHHCCCCCCCEEEEEEECC
PGEFEAGMSAEGQTREHILLAKTMGIDQIIVAVNKMDATEPPWSEKRYKQIVETLKKFMK
CCCCCCCCCCCCCCHHEEEEEECCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHH
GLGFKVDEIPFVPVSAWTGDNIIKRSENMPWYKGPTLVEALDNLKPPSVEKWAKLPLRIP
HCCCCCCCCCEEEEECCCCCCHHHHCCCCCCCCCCHHHHHHHCCCCCCHHHHHCCCEECC
IQDVYSITGVGTVPVGRVETGVLKVGDKVVFMPPGVGGEVRSIEMHHEKIEQAMPGDNIG
HHHHHHHCCCCCCCCCCCCCCEEEECCEEEEECCCCCCCEEEEHHHHHHHHHHCCCCCCC
FNVRGVSKNDIKRGDVAGHPENPPTVADQFTARVFVIWHPSAIAVGYTPVIHAHTASVAS
EEEECCCCCCCCCCCCCCCCCCCCCHHHCCCEEEEEEECCCEEEECCCCEEEHHHHHHHH
RIIEIKQKIDPRTGKVIEENPSFLKPGDAAVVVFKPLKPMVIEKFQEFQPLGRFAMRDMG
HHHHHHHHCCCCCCCEEECCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHCC
KTVGIGIVTDVKPAKVEIKMKK
CCCCEEEEECCCCCEEEEEECC
>Mature Secondary Structure 
SKKEKPHMNLIVIGHVDHGKSTLVGHLLYELGFVDEKTLKMLEEEAKKRGKESFKYAWL
CCCCCCCEEEEEEEECCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHH
LDKLKEERERGVTIDLTFMKFETPKYYFTIIDAPGHRDFIKNMITGASQADAAILVVSAR
HHHHHHHHHCCCEEEEEEEEEECCEEEEEEEECCCCHHHHHHHHCCCCCCCEEEEEEECC
PGEFEAGMSAEGQTREHILLAKTMGIDQIIVAVNKMDATEPPWSEKRYKQIVETLKKFMK
CCCCCCCCCCCCCCHHEEEEEECCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHH
GLGFKVDEIPFVPVSAWTGDNIIKRSENMPWYKGPTLVEALDNLKPPSVEKWAKLPLRIP
HCCCCCCCCCEEEEECCCCCCHHHHCCCCCCCCCCHHHHHHHCCCCCCHHHHHCCCEECC
IQDVYSITGVGTVPVGRVETGVLKVGDKVVFMPPGVGGEVRSIEMHHEKIEQAMPGDNIG
HHHHHHHCCCCCCCCCCCCCCEEEECCEEEEECCCCCCCEEEEHHHHHHHHHHCCCCCCC
FNVRGVSKNDIKRGDVAGHPENPPTVADQFTARVFVIWHPSAIAVGYTPVIHAHTASVAS
EEEECCCCCCCCCCCCCCCCCCCCCHHHCCCEEEEEEECCCEEEECCCCEEEHHHHHHHH
RIIEIKQKIDPRTGKVIEENPSFLKPGDAAVVVFKPLKPMVIEKFQEFQPLGRFAMRDMG
HHHHHHHHCCCCCCCEEECCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHCC
KTVGIGIVTDVKPAKVEIKMKK
CCCCEEEEECCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA