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The map label for this gene is gltX

Identifier: 156937898

GI number: 156937898

Start: 990540

End: 992228

Strand: Reverse

Name: gltX

Synonym: Igni_1110

Alternate gene names: 156937898

Gene position: 992228-990540 (Counterclockwise)

Preceding gene: 156937899

Following gene: 156937897

Centisome position: 76.47

GC content: 59.86

Gene sequence:

>1689_bases
GTGGACGTCAGGGAGCTGGCCCTCAAGCACGCCTTGAGGAACGCCTACCTCCACGGCGGCAAGGCCCAGCTCAAGCCGGT
GGTCACTGCGGTCCTAGCGGAGAGCCCGGAGCTCCGCCCTAAGGTTAAGGAGATAATACCAATAATCAAGGAAGTTGTAG
AAGAAGTTAACAGGATGAGCTTGGAGGAGCAAGAGAGGATCTTGAAGGAGAGGTTCCCAGAAGCGTTGGAGGAGAGGAAG
AAGGAGACTAGGAAGGGCTTGCCCCCTCTCCCCAACGCGGAGAGGGGCAAGGTCAAGACCCGCTTCGCCCCCAACCCCGA
CTTCTACATGACCTTGGGGAACGCGAGGCCCGCAATAATAAGTTACGAATACGCCAAGGCCTACGAGGGGAGGTTCGTCC
TTCGCTTCGAGGACACGGACCCGAGGACCAAGAGGCCCTTGCCGGAGGCCTACGAGGCCATAAAGGAGGACTTGAGCTGG
TTGGGGATAGGGTGGGACGAGGAGTACTACCAGTCCCAGAGGATGGAGGTTTACTACGGGCTCTTGAGGGAGCTCGTGAG
GAGGGGAGGGGCTTACGTCTGCACTTGCCCCCCGGAGGAGTGGAGGAAGCTCAGGGACGAGGGCAAGCCTTGCCCCCACA
GAGACTTGCCTCCGGAGGAGCAAGAGGAGCTGCTCGACCAAGTGCTAGAGGGCAAGTTCGGCGAGGGCGAAGCGGTGGTA
AGGGTCAAGACCGACTTGAGGCACCCGGACCCCTCGGTTAGGGACTGGGTGGCGTTTAGGATAATAGACACCTCCAAACA
CCCCCACCCGCTAACGGGCGACAAGTACGTCTTGTGGCCCACCTACAACTTCGCCGCGGGGGTGGACGATTACCTAATGG
GCATAACTCACGTGCTCAGGGCCAGGGAGCACAGGCAGAACACGGTGAAGCAAGAGTTCTTGTACAAACACTTGGGATGG
ACCATGCCTACGGTAATACACTTCGGGAGGCTCAAGCTCGAGGGCTTCGTGCTCTCCAAGAGCAAGATGAGGGAGGCCGG
CTTCAAGGGCGACGACCCCAGGGCAGCGACCATAAGGGGGCTGAGGAGGAGGGGCTTCGCCCCGGAGGCGATAAGGGAAG
TCATGCTGAGCGTCGGGATAAAGAGCAGCGACGCGAGCATAAGCTTCGCAAATTTAGCAGCCGAGAACAAGAAGGTGATA
GACAAGAAGGCCTACAGGGTGATGGCAGTGGAGGACCCCGCGCCGGCCAAGCTGCTGGCCCCAGAGCCCTTGGAGGCGGA
GCTCCCGTGGCACCCGACGGAGGGCTTGGGGTCTAGGAAGTACTCGGTGGAGCCCGGGGAGGTGGTCTACTTGGAGAGGG
CCGACTTGAGGAGGGCGAAGAGGAGAGGCGGGCTGAGGCTCATGGAGTTGGCCAACTTCAAGTACAAGGGCTTTGACGAG
GACGAGGGCGTCTACTTACTCGAGTTCGAGAGCAAGGAACTGGACAAGGAGAAGGGCTACGACATAGTCCAGTGGGTCAA
GGACCCCAAGGGGGCTATAGTCTGGAGGCCCGGGGAGAGGAAGTCCTTTGCCTTGGTGGAGCCAGAAGCCCTCAAGTTGG
AGGGCTGGGTACAGCTGATTAGGAAGGGCTACGCCAAGGTGGACGGGGTGGAGGAGGACACCTTGAACTTGATATACCTC
CACGAGTGA

Upstream 100 bases:

>100_bases
CTGCGCTAGTAATCGGGGGAAGGACCTCGTCGACTCCCAACAAGTATAGGGGCTTAGGGAGTTTTAGGACCTCCCCGGGA
CCCTTCAGAGGGAAATTAGA

Downstream 100 bases:

>100_bases
AGGGTGTTCGGTTTTGAAGGCCCTCGTGGTGGGGGGCTTCGGGGGGCACGCGGGCCAAGCGTTCGCGATAGCTTACTGGC
TCAAGGAGCTCGGCTCCCCT

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase; GluRS

Number of amino acids: Translated: 562; Mature: 562

Protein sequence:

>562_residues
MDVRELALKHALRNAYLHGGKAQLKPVVTAVLAESPELRPKVKEIIPIIKEVVEEVNRMSLEEQERILKERFPEALEERK
KETRKGLPPLPNAERGKVKTRFAPNPDFYMTLGNARPAIISYEYAKAYEGRFVLRFEDTDPRTKRPLPEAYEAIKEDLSW
LGIGWDEEYYQSQRMEVYYGLLRELVRRGGAYVCTCPPEEWRKLRDEGKPCPHRDLPPEEQEELLDQVLEGKFGEGEAVV
RVKTDLRHPDPSVRDWVAFRIIDTSKHPHPLTGDKYVLWPTYNFAAGVDDYLMGITHVLRAREHRQNTVKQEFLYKHLGW
TMPTVIHFGRLKLEGFVLSKSKMREAGFKGDDPRAATIRGLRRRGFAPEAIREVMLSVGIKSSDASISFANLAAENKKVI
DKKAYRVMAVEDPAPAKLLAPEPLEAELPWHPTEGLGSRKYSVEPGEVVYLERADLRRAKRRGGLRLMELANFKYKGFDE
DEGVYLLEFESKELDKEKGYDIVQWVKDPKGAIVWRPGERKSFALVEPEALKLEGWVQLIRKGYAKVDGVEEDTLNLIYL
HE

Sequences:

>Translated_562_residues
MDVRELALKHALRNAYLHGGKAQLKPVVTAVLAESPELRPKVKEIIPIIKEVVEEVNRMSLEEQERILKERFPEALEERK
KETRKGLPPLPNAERGKVKTRFAPNPDFYMTLGNARPAIISYEYAKAYEGRFVLRFEDTDPRTKRPLPEAYEAIKEDLSW
LGIGWDEEYYQSQRMEVYYGLLRELVRRGGAYVCTCPPEEWRKLRDEGKPCPHRDLPPEEQEELLDQVLEGKFGEGEAVV
RVKTDLRHPDPSVRDWVAFRIIDTSKHPHPLTGDKYVLWPTYNFAAGVDDYLMGITHVLRAREHRQNTVKQEFLYKHLGW
TMPTVIHFGRLKLEGFVLSKSKMREAGFKGDDPRAATIRGLRRRGFAPEAIREVMLSVGIKSSDASISFANLAAENKKVI
DKKAYRVMAVEDPAPAKLLAPEPLEAELPWHPTEGLGSRKYSVEPGEVVYLERADLRRAKRRGGLRLMELANFKYKGFDE
DEGVYLLEFESKELDKEKGYDIVQWVKDPKGAIVWRPGERKSFALVEPEALKLEGWVQLIRKGYAKVDGVEEDTLNLIYL
HE
>Mature_562_residues
MDVRELALKHALRNAYLHGGKAQLKPVVTAVLAESPELRPKVKEIIPIIKEVVEEVNRMSLEEQERILKERFPEALEERK
KETRKGLPPLPNAERGKVKTRFAPNPDFYMTLGNARPAIISYEYAKAYEGRFVLRFEDTDPRTKRPLPEAYEAIKEDLSW
LGIGWDEEYYQSQRMEVYYGLLRELVRRGGAYVCTCPPEEWRKLRDEGKPCPHRDLPPEEQEELLDQVLEGKFGEGEAVV
RVKTDLRHPDPSVRDWVAFRIIDTSKHPHPLTGDKYVLWPTYNFAAGVDDYLMGITHVLRAREHRQNTVKQEFLYKHLGW
TMPTVIHFGRLKLEGFVLSKSKMREAGFKGDDPRAATIRGLRRRGFAPEAIREVMLSVGIKSSDASISFANLAAENKKVI
DKKAYRVMAVEDPAPAKLLAPEPLEAELPWHPTEGLGSRKYSVEPGEVVYLERADLRRAKRRGGLRLMELANFKYKGFDE
DEGVYLLEFESKELDKEKGYDIVQWVKDPKGAIVWRPGERKSFALVEPEALKLEGWVQLIRKGYAKVDGVEEDTLNLIYL
HE

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu)

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI62241042, Length=439, Percent_Identity=29.3849658314351, Blast_Score=177, Evalue=2e-44,
Organism=Homo sapiens, GI4826960, Length=381, Percent_Identity=33.3333333333333, Blast_Score=174, Evalue=3e-43,
Organism=Homo sapiens, GI134288884, Length=298, Percent_Identity=31.5436241610738, Blast_Score=132, Evalue=8e-31,
Organism=Escherichia coli, GI1786895, Length=509, Percent_Identity=30.8447937131631, Blast_Score=192, Evalue=6e-50,
Organism=Escherichia coli, GI1788743, Length=369, Percent_Identity=28.9972899728997, Blast_Score=134, Evalue=2e-32,
Organism=Escherichia coli, GI226510925, Length=282, Percent_Identity=30.1418439716312, Blast_Score=94, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17511007, Length=437, Percent_Identity=29.7482837528604, Blast_Score=176, Evalue=3e-44,
Organism=Caenorhabditis elegans, GI17543332, Length=375, Percent_Identity=30.9333333333333, Blast_Score=155, Evalue=5e-38,
Organism=Caenorhabditis elegans, GI71993394, Length=310, Percent_Identity=30.3225806451613, Blast_Score=102, Evalue=4e-22,
Organism=Saccharomyces cerevisiae, GI6324742, Length=379, Percent_Identity=33.7730870712401, Blast_Score=202, Evalue=9e-53,
Organism=Saccharomyces cerevisiae, GI6321192, Length=516, Percent_Identity=29.4573643410853, Blast_Score=180, Evalue=5e-46,
Organism=Saccharomyces cerevisiae, GI6324540, Length=297, Percent_Identity=35.016835016835, Blast_Score=124, Evalue=3e-29,
Organism=Drosophila melanogaster, GI24649466, Length=470, Percent_Identity=28.7234042553192, Blast_Score=170, Evalue=2e-42,
Organism=Drosophila melanogaster, GI17864488, Length=438, Percent_Identity=30.1369863013699, Blast_Score=165, Evalue=1e-40,
Organism=Drosophila melanogaster, GI24665321, Length=304, Percent_Identity=35.5263157894737, Blast_Score=142, Evalue=6e-34,

Paralogues:

None

Copy number: 520 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1200 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYE_IGNH4 (A8ABI5)

Other databases:

- EMBL:   CP000816
- RefSeq:   YP_001435694.1
- STRING:   A8ABI5
- GeneID:   5562106
- GenomeReviews:   CP000816_GR
- KEGG:   iho:Igni_1110
- eggNOG:   arNOG04636
- HOGENOM:   HBG334108
- OMA:   MRFAPNP
- BioCyc:   IHOS453591:IGNI_1110-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00022_A
- InterPro:   IPR004526
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR020059
- InterPro:   IPR022888
- InterPro:   IPR011035
- InterPro:   IPR014729
- Gene3D:   G3DSA:1.10.1160.10
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR10119
- PRINTS:   PR00987
- TIGRFAMs:   TIGR00463

Pfam domain/function: PF00749 tRNA-synt_1c; PF03950 tRNA-synt_1c_C; SSF50715 Ribosomal_L25rel

EC number: =6.1.1.17

Molecular weight: Translated: 64686; Mature: 64686

Theoretical pI: Translated: 7.73; Mature: 7.73

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDVRELALKHALRNAYLHGGKAQLKPVVTAVLAESPELRPKVKEIIPIIKEVVEEVNRMS
CCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCC
LEEQERILKERFPEALEERKKETRKGLPPLPNAERGKVKTRFAPNPDFYMTLGNARPAII
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCEEEEECCCCCEEE
SYEYAKAYEGRFVLRFEDTDPRTKRPLPEAYEAIKEDLSWLGIGWDEEYYQSQRMEVYYG
EEHHHHHHCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
LLRELVRRGGAYVCTCPPEEWRKLRDEGKPCPHRDLPPEEQEELLDQVLEGKFGEGEAVV
HHHHHHHCCCCEEEECCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCEEE
RVKTDLRHPDPSVRDWVAFRIIDTSKHPHPLTGDKYVLWPTYNFAAGVDDYLMGITHVLR
EEEECCCCCCCCHHHEEEEEEEECCCCCCCCCCCCEEEEECCCHHCCHHHHHHHHHHHHH
AREHRQNTVKQEFLYKHLGWTMPTVIHFGRLKLEGFVLSKSKMREAGFKGDDPRAATIRG
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHEEEEEEEECHHHHHHCCCCCCCCHHHHHHH
LRRRGFAPEAIREVMLSVGIKSSDASISFANLAAENKKVIDKKAYRVMAVEDPAPAKLLA
HHHCCCCHHHHHHHHHHCCCCCCCCCEEHHHHHHCCHHHHHHHCEEEEEECCCCCCCCCC
PEPLEAELPWHPTEGLGSRKYSVEPGEVVYLERADLRRAKRRGGLRLMELANFKYKGFDE
CCCCCCCCCCCCCCCCCCCEECCCCCCEEEEEHHHHHHHHHHCCEEEEECCCCCCCCCCC
DEGVYLLEFESKELDKEKGYDIVQWVKDPKGAIVWRPGERKSFALVEPEALKLEGWVQLI
CCCEEEEEECCHHCCHHCCCHHHHHHCCCCCEEEECCCCCCCEEEECCHHHHHHHHHHHH
RKGYAKVDGVEEDTLNLIYLHE
HHHHHHCCCCCCCCEEEEEEEC
>Mature Secondary Structure
MDVRELALKHALRNAYLHGGKAQLKPVVTAVLAESPELRPKVKEIIPIIKEVVEEVNRMS
CCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCC
LEEQERILKERFPEALEERKKETRKGLPPLPNAERGKVKTRFAPNPDFYMTLGNARPAII
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCEEEEECCCCCEEE
SYEYAKAYEGRFVLRFEDTDPRTKRPLPEAYEAIKEDLSWLGIGWDEEYYQSQRMEVYYG
EEHHHHHHCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
LLRELVRRGGAYVCTCPPEEWRKLRDEGKPCPHRDLPPEEQEELLDQVLEGKFGEGEAVV
HHHHHHHCCCCEEEECCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCEEE
RVKTDLRHPDPSVRDWVAFRIIDTSKHPHPLTGDKYVLWPTYNFAAGVDDYLMGITHVLR
EEEECCCCCCCCHHHEEEEEEEECCCCCCCCCCCCEEEEECCCHHCCHHHHHHHHHHHHH
AREHRQNTVKQEFLYKHLGWTMPTVIHFGRLKLEGFVLSKSKMREAGFKGDDPRAATIRG
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHEEEEEEEECHHHHHHCCCCCCCCHHHHHHH
LRRRGFAPEAIREVMLSVGIKSSDASISFANLAAENKKVIDKKAYRVMAVEDPAPAKLLA
HHHCCCCHHHHHHHHHHCCCCCCCCCEEHHHHHHCCHHHHHHHCEEEEEECCCCCCCCCC
PEPLEAELPWHPTEGLGSRKYSVEPGEVVYLERADLRRAKRRGGLRLMELANFKYKGFDE
CCCCCCCCCCCCCCCCCCCEECCCCCCEEEEEHHHHHHHHHHCCEEEEECCCCCCCCCCC
DEGVYLLEFESKELDKEKGYDIVQWVKDPKGAIVWRPGERKSFALVEPEALKLEGWVQLI
CCCEEEEEECCHHCCHHCCCHHHHHHCCCCCEEEECCCCCCCEEEECCHHHHHHHHHHHH
RKGYAKVDGVEEDTLNLIYLHE
HHHHHHCCCCCCCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA