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The map label for this gene is top6B [H]

Identifier: 156937878

GI number: 156937878

Start: 971369

End: 973030

Strand: Reverse

Name: top6B [H]

Synonym: Igni_1090

Alternate gene names: 156937878

Gene position: 973030-971369 (Counterclockwise)

Preceding gene: 156937879

Following gene: 156937876

Centisome position: 74.99

GC content: 56.44

Gene sequence:

>1662_bases
ATGCCCAAGCAGCGCGTCCAGAGCGAGAGGGGCGAGGAGCGCTTCAAGGCAATCGGGCCGGCGGAGTTCTTCTTCCGCAA
CATAGAGCTCACCGGGTTCGACAACTACACGAGGGCGATATTCCAAACCGTTAAGGAAATGTTGGACAACTCCTTAGACG
CCACAGAAACTCACGGAATACTGCCCAGAATAACTATAGTTATCGAGAGGCTGAATGAGGCAAAGGCTTCGAAGGAAGAG
GAAGAAAAGAAGGAGATCAAGGGCTCCAAGGACGGAATTTACAGAATAACGGTGGACGACAACGGGATCGGGGTGCCGCA
AGACAAGATACCTTACGCCTTCGGCAGGATGCTGTACAGCTCCAAGTACGTGGAGAGGCAGACCAGAGGAACCTACGGCC
TAGGTGTGAAGGCCGCGGTCCTCTACGCGCAGAAGACCACCGGCGAGCCCGCCACGGTAATTTCCAGCCCTATAAAGGAC
AAAATGACCTACAGGTTCAAGGTGTCCACTAACATAAGCACCAACGAGCCGGACGTGAAGGAAAGGGGCTCGTGGACAAA
GTCCGGCGACTGGCACGGGACCAGGGTGAGCGTAATCATAGAGGGGAACTGGAGGAGGGCGAGGAGTAAGGTCGTAGAGT
ACGTCCGGAGGACTGCCATCGTGGCGCCCTACGCGGACATAACCCTAATCACGCCCGACGGGACGTTAATTAGATACCCC
AGAAGGACCAAGGAAATGCCCAAGCCCCCCAAGGAGGTGAAGCCCCACCCGCTGGGCATAGATATAGAAACTATGAAGAG
GATAGCGCAGCTGACCACTGCGAAGACCTTGCTCCAGATGTTGACCACCGAGTTCCAAGCGGTGGGTGCGAAGACCGCTA
AGGCGATATTGAGCATGGCCGGCTTGGAGCCGGACCTAGAGCCGAAGAAGCTTACGGAGGAACAGATCAAAGCCTTGGTC
AACGCGATGAAAGAGTACGGGACGACCAAGAGGTACAAGGCCCCCAGCAAGGAAGCCCTCTCGCCTATCGGGGAGAAGCT
CATAAAGATAGGCCTCAAGAGCATGTTGAACCCGGAGTTCGTAACTGCGGTGAGCAGGAGGCCCTCCTCCTACTCCGGCC
ACCCGTTCGTGGTGGAGGTGGGCATAGCTTACGGAGGCGAAATACCCCCCTCCGACGCCCCGGTCTTGCTTAGGTACGCC
AACAAGACCCCCCTCCTCTACAATGAGAGGAACGACGTGAGCTGGAAGGTGGTGAGCGAGCTCAACTGGAAGAACTACGA
GGTCTCCTTCCCCGCCCCCTTAGTGGTCTTGGTCCACGTGGCGAGTACCAAGGTCCCGTTTAAGGACGTGAGCAAGGACA
CCATAGCGGACGTGCCGGAGATAGAGGCCGAGATAAGGCTCGCCCTCCAAGAGGCGGCGAGGAGGCTGAAGAGGTTCATA
ATTAAGAAGAGGAAGGAAGAGGAGCTCGCGGAGAAGGTAATAACCTTCCTCAAGTACGTGCCGGAGATAGCGAGGAGCTT
GAAGGTCTTGAGCGTGGACCCAGAGACCAGGAAGCCCACGGTGAGCGAGGAGGAGATAGAGGAGAAGCTAAAGAAGTTGA
TAGCGAAAAAGCTGGGGGTTCCGGAGGACAAGCTCCCGGAGGTGGTGATAGGAATAGAGTGA

Upstream 100 bases:

>100_bases
GGAGAGGTTCAAGAAGTACTTGAAGCTGGCCTTGGAGCTCTGAGCCCTACGTCCTTTTTAAACTAATAAGGAAGCTCCCG
AGACTACAAAGGGCTTAGAA

Downstream 100 bases:

>100_bases
GCGGCTCGGGAGGGGTCCGGCAGCTCTTCACCGATCCGAAAGACCGGACCCTCTTCATCGCCCCCCGCCGACTCCGGCAA
TCCTACAGCTGAGCTCCGGC

Product: DNA topoisomerase VI subunit B

Products: NA

Alternate protein names: Type II DNA topoisomerase VI subunit B; TopoVI-B [H]

Number of amino acids: Translated: 553; Mature: 552

Protein sequence:

>553_residues
MPKQRVQSERGEERFKAIGPAEFFFRNIELTGFDNYTRAIFQTVKEMLDNSLDATETHGILPRITIVIERLNEAKASKEE
EEKKEIKGSKDGIYRITVDDNGIGVPQDKIPYAFGRMLYSSKYVERQTRGTYGLGVKAAVLYAQKTTGEPATVISSPIKD
KMTYRFKVSTNISTNEPDVKERGSWTKSGDWHGTRVSVIIEGNWRRARSKVVEYVRRTAIVAPYADITLITPDGTLIRYP
RRTKEMPKPPKEVKPHPLGIDIETMKRIAQLTTAKTLLQMLTTEFQAVGAKTAKAILSMAGLEPDLEPKKLTEEQIKALV
NAMKEYGTTKRYKAPSKEALSPIGEKLIKIGLKSMLNPEFVTAVSRRPSSYSGHPFVVEVGIAYGGEIPPSDAPVLLRYA
NKTPLLYNERNDVSWKVVSELNWKNYEVSFPAPLVVLVHVASTKVPFKDVSKDTIADVPEIEAEIRLALQEAARRLKRFI
IKKRKEEELAEKVITFLKYVPEIARSLKVLSVDPETRKPTVSEEEIEEKLKKLIAKKLGVPEDKLPEVVIGIE

Sequences:

>Translated_553_residues
MPKQRVQSERGEERFKAIGPAEFFFRNIELTGFDNYTRAIFQTVKEMLDNSLDATETHGILPRITIVIERLNEAKASKEE
EEKKEIKGSKDGIYRITVDDNGIGVPQDKIPYAFGRMLYSSKYVERQTRGTYGLGVKAAVLYAQKTTGEPATVISSPIKD
KMTYRFKVSTNISTNEPDVKERGSWTKSGDWHGTRVSVIIEGNWRRARSKVVEYVRRTAIVAPYADITLITPDGTLIRYP
RRTKEMPKPPKEVKPHPLGIDIETMKRIAQLTTAKTLLQMLTTEFQAVGAKTAKAILSMAGLEPDLEPKKLTEEQIKALV
NAMKEYGTTKRYKAPSKEALSPIGEKLIKIGLKSMLNPEFVTAVSRRPSSYSGHPFVVEVGIAYGGEIPPSDAPVLLRYA
NKTPLLYNERNDVSWKVVSELNWKNYEVSFPAPLVVLVHVASTKVPFKDVSKDTIADVPEIEAEIRLALQEAARRLKRFI
IKKRKEEELAEKVITFLKYVPEIARSLKVLSVDPETRKPTVSEEEIEEKLKKLIAKKLGVPEDKLPEVVIGIE
>Mature_552_residues
PKQRVQSERGEERFKAIGPAEFFFRNIELTGFDNYTRAIFQTVKEMLDNSLDATETHGILPRITIVIERLNEAKASKEEE
EKKEIKGSKDGIYRITVDDNGIGVPQDKIPYAFGRMLYSSKYVERQTRGTYGLGVKAAVLYAQKTTGEPATVISSPIKDK
MTYRFKVSTNISTNEPDVKERGSWTKSGDWHGTRVSVIIEGNWRRARSKVVEYVRRTAIVAPYADITLITPDGTLIRYPR
RTKEMPKPPKEVKPHPLGIDIETMKRIAQLTTAKTLLQMLTTEFQAVGAKTAKAILSMAGLEPDLEPKKLTEEQIKALVN
AMKEYGTTKRYKAPSKEALSPIGEKLIKIGLKSMLNPEFVTAVSRRPSSYSGHPFVVEVGIAYGGEIPPSDAPVLLRYAN
KTPLLYNERNDVSWKVVSELNWKNYEVSFPAPLVVLVHVASTKVPFKDVSKDTIADVPEIEAEIRLALQEAARRLKRFII
KKRKEEELAEKVITFLKYVPEIARSLKVLSVDPETRKPTVSEEEIEEKLKKLIAKKLGVPEDKLPEVVIGIE

Specific function: Relaxes both positive and negative superturns and exhibits a strong decatenase activity. The B subunit binds ATP [H]

COG id: COG1389

COG function: function code L; DNA topoisomerase VI, subunit B

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TOP6B family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR010979
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR005734
- InterPro:   IPR015320
- ProDom:   PD013790 [H]

Pfam domain/function: PF02518 HATPase_c; PF09239 Topo-VIb_trans [H]

EC number: =5.99.1.3 [H]

Molecular weight: Translated: 62327; Mature: 62196

Theoretical pI: Translated: 9.95; Mature: 9.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKQRVQSERGEERFKAIGPAEFFFRNIELTGFDNYTRAIFQTVKEMLDNSLDATETHGI
CCCHHHHHHHHHHHHHHCCCHHHHHEECEEECCCHHHHHHHHHHHHHHCCCCCCHHHCCC
LPRITIVIERLNEAKASKEEEEKKEIKGSKDGIYRITVDDNGIGVPQDKIPYAFGRMLYS
HHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCEEEEEECCCCCCCCCHHCCHHHHHHHHH
SKYVERQTRGTYGLGVKAAVLYAQKTTGEPATVISSPIKDKMTYRFKVSTNISTNEPDVK
HHHHHHHHCCCCCCCHHHEEEEEECCCCCCHHHHHCCHHCCEEEEEEEECCCCCCCCCHH
ERGSWTKSGDWHGTRVSVIIEGNWRRARSKVVEYVRRTAIVAPYADITLITPDGTLIRYP
HCCCCCCCCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHEEECCCCEEEECCCCCEEECC
RRTKEMPKPPKEVKPHPLGIDIETMKRIAQLTTAKTLLQMLTTEFQAVGAKTAKAILSMA
CHHCCCCCCCHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GLEPDLEPKKLTEEQIKALVNAMKEYGTTKRYKAPSKEALSPIGEKLIKIGLKSMLNPEF
CCCCCCCHHHCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHH
VTAVSRRPSSYSGHPFVVEVGIAYGGEIPPSDAPVLLRYANKTPLLYNERNDVSWKVVSE
HHHHHCCCCCCCCCCEEEEEEECCCCCCCCCCCCEEEEECCCCCEEEECCCCCCEEEEEC
LNWKNYEVSFPAPLVVLVHVASTKVPFKDVSKDTIADVPEIEAEIRLALQEAARRLKRFI
CCCCCEEEECCCHHHHHEEHHCCCCCHHHCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHH
IKKRKEEELAEKVITFLKYVPEIARSLKVLSVDPETRKPTVSEEEIEEKLKKLIAKKLGV
HHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCC
PEDKLPEVVIGIE
CCCCCCCEEEECC
>Mature Secondary Structure 
PKQRVQSERGEERFKAIGPAEFFFRNIELTGFDNYTRAIFQTVKEMLDNSLDATETHGI
CCHHHHHHHHHHHHHHCCCHHHHHEECEEECCCHHHHHHHHHHHHHHCCCCCCHHHCCC
LPRITIVIERLNEAKASKEEEEKKEIKGSKDGIYRITVDDNGIGVPQDKIPYAFGRMLYS
HHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCEEEEEECCCCCCCCCHHCCHHHHHHHHH
SKYVERQTRGTYGLGVKAAVLYAQKTTGEPATVISSPIKDKMTYRFKVSTNISTNEPDVK
HHHHHHHHCCCCCCCHHHEEEEEECCCCCCHHHHHCCHHCCEEEEEEEECCCCCCCCCHH
ERGSWTKSGDWHGTRVSVIIEGNWRRARSKVVEYVRRTAIVAPYADITLITPDGTLIRYP
HCCCCCCCCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHEEECCCCEEEECCCCCEEECC
RRTKEMPKPPKEVKPHPLGIDIETMKRIAQLTTAKTLLQMLTTEFQAVGAKTAKAILSMA
CHHCCCCCCCHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GLEPDLEPKKLTEEQIKALVNAMKEYGTTKRYKAPSKEALSPIGEKLIKIGLKSMLNPEF
CCCCCCCHHHCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHH
VTAVSRRPSSYSGHPFVVEVGIAYGGEIPPSDAPVLLRYANKTPLLYNERNDVSWKVVSE
HHHHHCCCCCCCCCCEEEEEEECCCCCCCCCCCCEEEEECCCCCEEEECCCCCCEEEEEC
LNWKNYEVSFPAPLVVLVHVASTKVPFKDVSKDTIADVPEIEAEIRLALQEAARRLKRFI
CCCCCEEEECCCHHHHHEEHHCCCCCHHHCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHH
IKKRKEEELAEKVITFLKYVPEIARSLKVLSVDPETRKPTVSEEEIEEKLKKLIAKKLGV
HHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCC
PEDKLPEVVIGIE
CCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10382966 [H]