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The map label for this gene is rsmA [H]

Identifier: 156937848

GI number: 156937848

Start: 944000

End: 944731

Strand: Reverse

Name: rsmA [H]

Synonym: Igni_1059

Alternate gene names: 156937848

Gene position: 944731-944000 (Counterclockwise)

Preceding gene: 156937849

Following gene: 156937847

Centisome position: 72.81

GC content: 57.92

Gene sequence:

>732_bases
TTGCTGAAGTGGACAAAGGCCAAGCTCGCCGAGCTCGGTATAAGGCCCAGCAAGAAGATGGGGCAGAACTTCACGGTAAA
CCCCAAAATAATAGAGTTCTTCTTGAGCGAGGTCCCCTCGGGGGAAGTTGTAATAGAAATAGGGGCCGGCCTCGGGGCCC
TCACGGCCCCGCTCTCCAAAGTTTCTAAGAAGGTAATTGCAATAGAGAAGGATCTCAGGTTGTGTAACTATTTGAAATCC
TTAAACTTGGAGAACGTGGAGGTCGTATGCGGCGACGCGTTAGAGCTGGAGCTCGACGCTCCGGTCGTAGTGGGCTCCCT
CCCTTATAGCATAAGCGGGCCCCTCCTCGCGAAGCTCTTCACAGAAGGTCGGTGGAACAAGGGGGTGTTCCTCCTTCAGA
AGGAGGTCGCGGAGAGGCTGGTCGCCGAGCCCGGGACGAAGGAGTACGGGAGGCTAACCGTGTTGGCCTCCTTGTGCTGC
GAGGCCCGCTTGGGCCCGGTGTGGGGCCCGGAGTCCTTTTACCCCAAGCCGGAGGTCCTAAGCCGCCACGTAATATTAAT
TAAAAAGAGGTCCGTCCCGAAGGAGTTCTCAGAATTCTTGGCGTGCGTCTTCTCGCAGAGGAACAAGAAGGCCCGTAAGG
TCTTGCCCTCTTGCGGCGCCCGCTGGGAGGGGGAAGAGAGGGTGAGGGAGCTCCCTCCCGAGAGCTTGTGGGAGGCTTGG
GAGGAGCGTTAG

Upstream 100 bases:

>100_bases
AAAGGAGAGGAGAAGTACTACTTGTTCGTAGAGCCCCCGAAGCCGGGCTATACCTACTTGAACTACTTGGGGAGGCTGCG
AAGGGCGGCGAGGGGTTAGC

Downstream 100 bases:

>100_bases
CGCTTTATTCTCTTTTACGCCTCTGGACCGCGGATCAAGCCTTGCAAGAAGTGAGGAAGGTCTTGGTCGTCGGAGCCGGT
GTCATGGGCCACGGAATAGC

Product: dimethyladenosine transferase

Products: NA

Alternate protein names: 16S rRNA dimethyladenosine transferase; 16S rRNA dimethylase; S-adenosylmethionine-6-N',N'-adenosyl(rRNA) dimethyltransferase [H]

Number of amino acids: Translated: 243; Mature: 243

Protein sequence:

>243_residues
MLKWTKAKLAELGIRPSKKMGQNFTVNPKIIEFFLSEVPSGEVVIEIGAGLGALTAPLSKVSKKVIAIEKDLRLCNYLKS
LNLENVEVVCGDALELELDAPVVVGSLPYSISGPLLAKLFTEGRWNKGVFLLQKEVAERLVAEPGTKEYGRLTVLASLCC
EARLGPVWGPESFYPKPEVLSRHVILIKKRSVPKEFSEFLACVFSQRNKKARKVLPSCGARWEGEERVRELPPESLWEAW
EER

Sequences:

>Translated_243_residues
MLKWTKAKLAELGIRPSKKMGQNFTVNPKIIEFFLSEVPSGEVVIEIGAGLGALTAPLSKVSKKVIAIEKDLRLCNYLKS
LNLENVEVVCGDALELELDAPVVVGSLPYSISGPLLAKLFTEGRWNKGVFLLQKEVAERLVAEPGTKEYGRLTVLASLCC
EARLGPVWGPESFYPKPEVLSRHVILIKKRSVPKEFSEFLACVFSQRNKKARKVLPSCGARWEGEERVRELPPESLWEAW
EER
>Mature_243_residues
MLKWTKAKLAELGIRPSKKMGQNFTVNPKIIEFFLSEVPSGEVVIEIGAGLGALTAPLSKVSKKVIAIEKDLRLCNYLKS
LNLENVEVVCGDALELELDAPVVVGSLPYSISGPLLAKLFTEGRWNKGVFLLQKEVAERLVAEPGTKEYGRLTVLASLCC
EARLGPVWGPESFYPKPEVLSRHVILIKKRSVPKEFSEFLACVFSQRNKKARKVLPSCGARWEGEERVRELPPESLWEAW
EER

Specific function: Specifically dimethylates two adjacent adenosines in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits [H]

COG id: COG0030

COG function: function code J; Dimethyladenosine transferase (rRNA methylation)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657198, Length=231, Percent_Identity=29.4372294372294, Blast_Score=80, Evalue=1e-15,
Organism=Homo sapiens, GI156415992, Length=199, Percent_Identity=29.6482412060301, Blast_Score=74, Evalue=1e-13,
Organism=Escherichia coli, GI1786236, Length=219, Percent_Identity=33.7899543378995, Blast_Score=97, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI25146882, Length=204, Percent_Identity=33.3333333333333, Blast_Score=84, Evalue=6e-17,
Organism=Saccharomyces cerevisiae, GI6324989, Length=206, Percent_Identity=29.126213592233, Blast_Score=77, Evalue=4e-15,
Organism=Drosophila melanogaster, GI21358017, Length=178, Percent_Identity=35.9550561797753, Blast_Score=89, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001737
- InterPro:   IPR020598
- InterPro:   IPR011530 [H]

Pfam domain/function: PF00398 RrnaAD [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 27133; Mature: 27133

Theoretical pI: Translated: 8.80; Mature: 8.80

Prosite motif: PS01131 RRNA_A_DIMETH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKWTKAKLAELGIRPSKKMGQNFTVNPKIIEFFLSEVPSGEVVIEIGAGLGALTAPLSK
CCCCHHHHHHHHCCCCCHHHCCCCCCCHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHH
VSKKVIAIEKDLRLCNYLKSLNLENVEVVCGDALELELDAPVVVGSLPYSISGPLLAKLF
HHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCEEEEECCCEEECCCCCCCCCHHHHHHH
TEGRWNKGVFLLQKEVAERLVAEPGTKEYGRLTVLASLCCEARLGPVWGPESFYPKPEVL
HCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHH
SRHVILIKKRSVPKEFSEFLACVFSQRNKKARKVLPSCGARWEGEERVRELPPESLWEAW
CCEEEEEECCCCCHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCHHHHHHCCHHHHHHHH
EER
HCC
>Mature Secondary Structure
MLKWTKAKLAELGIRPSKKMGQNFTVNPKIIEFFLSEVPSGEVVIEIGAGLGALTAPLSK
CCCCHHHHHHHHCCCCCHHHCCCCCCCHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHH
VSKKVIAIEKDLRLCNYLKSLNLENVEVVCGDALELELDAPVVVGSLPYSISGPLLAKLF
HHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCEEEEECCCEEECCCCCCCCCHHHHHHH
TEGRWNKGVFLLQKEVAERLVAEPGTKEYGRLTVLASLCCEARLGPVWGPESFYPKPEVL
HCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHH
SRHVILIKKRSVPKEFSEFLACVFSQRNKKARKVLPSCGARWEGEERVRELPPESLWEAW
CCEEEEEECCCCCHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCHHHHHHCCHHHHHHHH
EER
HCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Dimethylation [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10382966 [H]