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The map label for this gene is leuA

Identifier: 156937774

GI number: 156937774

Start: 875944

End: 877143

Strand: Reverse

Name: leuA

Synonym: Igni_0983

Alternate gene names: 156937774

Gene position: 877143-875944 (Counterclockwise)

Preceding gene: 156937777

Following gene: 156937767

Centisome position: 67.6

GC content: 58.5

Gene sequence:

>1200_bases
ATGCTTATTGTTGAGGGCGTAAGGATAGCGGAAGAGCTGGAAAACCTCCCCAAAGAGGTACGCATTTTTGATACCACCTT
ACGTGACGGCGAGCAGACCCCCGGAATAAGCTTCACCAAGGAACAGAAGCTGATGATCGCGAGACAGCTCGCGAAGCTAG
GAGTAGCGAGCATAGAGGCCGGCTTCCCGGCAGTGTCCCAAGGCGAGTTCGAGGCGGTTAAGGCGATTGCCCGAGAGGGA
CTGGGCCCGGAGATAGTGGCCCTAGCTAGGGCGAACAAGAGGGACATAGATAAGGCTCTGGACGCCGACGTGGACGCGAT
CCACGTGTTCATAGCAGCTTCGGACATCCACTTGAAGTACAAGCTTAGGATGACCAGAGAGGAAGCCCTCAGGAGGGCCG
TAGAGGCGGTGGAATACGCGAAGAGCCACGGGGTGACGGTGGAGTTCAGCCCCGAGGACGGCACCAGGGCAGACCTCAAC
TACCTCTACACTATGGTTGAAGCCGTAGTGGACGCGGGGGCGGACAGGGTGGACATACCCGATACCGTAGGAGTCATGAC
GCCCACCAGGATGAAGTACCTAATAAAGTTCATACTGCCGGCCGCCAAGGGGAGGATAGTCAGCGTCCACTGCCACAACG
ACTTCGGCTTGGCCGTAGCGAACAGCATAGCCGGCATAGAGGCCGGCGCCAGGCAAGCCCACGTGACCGTGAACGGGATA
GGGGAGAGGGCCGGCAACGCCGCGCTCGAGGAAGTGGTGGCTGCCCTAGAGTTCTTACTGAACGTGAGGACCGGCGTCAA
CACTAAGCTGCTCTACGAGACCAGCCTGCTGGTGTCCAAGATAACCGGCATACCGATACCTCCTAACAAGCCCATCGTCG
GCGAGAACGTCTTCAGTCACGAGTCCGGCATACACGTGCACGGGGTGATCAACAACCCGTTCACCTACGAGCCCATACAG
CCGGAAATGGTCGGCCAGAGGAGGAGGATCGTGCTCGGCAAGCACTCCGGCAGGCACGGCGTTGAATACGCCCTCAAGAC
CCTAGGCTACCCCACGGACCCTGACGTGGTCCTAAGGGTGTTAGACGAGGTTAAGAGGCTCGGCGACATGGGCATTAGGG
TTACCGAGGACAAGCTGAGGGAGATCGTAGAAAAGGTCTTAGAGGAGAGGGAGAGGGCTAAGGAAGCTGCAACCTCTTAA

Upstream 100 bases:

>100_bases
TTCGGCAACTCCTATCTCCCTTGATGGAGCAGAGCGTTGGGTTATGAGTGGAAAGCGCGCGGGTAGTTTTATAACGTCCT
CGGGAGACCCGCGTTAGGAG

Downstream 100 bases:

>100_bases
CGACCTCTTCTGCGAACTCGAAGGTGCCGTAGTTCCCGCCCAAGTCCGGCGTCTTTACTCCCTCTGACAAGGCCTTCTCT
ATCGCCCTCTGAAGCGCAGC

Product: isopropylmalate/citramalate/homocitrate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase; Alpha-isopropylmalate synthase

Number of amino acids: Translated: 399; Mature: 399

Protein sequence:

>399_residues
MLIVEGVRIAEELENLPKEVRIFDTTLRDGEQTPGISFTKEQKLMIARQLAKLGVASIEAGFPAVSQGEFEAVKAIAREG
LGPEIVALARANKRDIDKALDADVDAIHVFIAASDIHLKYKLRMTREEALRRAVEAVEYAKSHGVTVEFSPEDGTRADLN
YLYTMVEAVVDAGADRVDIPDTVGVMTPTRMKYLIKFILPAAKGRIVSVHCHNDFGLAVANSIAGIEAGARQAHVTVNGI
GERAGNAALEEVVAALEFLLNVRTGVNTKLLYETSLLVSKITGIPIPPNKPIVGENVFSHESGIHVHGVINNPFTYEPIQ
PEMVGQRRRIVLGKHSGRHGVEYALKTLGYPTDPDVVLRVLDEVKRLGDMGIRVTEDKLREIVEKVLEERERAKEAATS

Sequences:

>Translated_399_residues
MLIVEGVRIAEELENLPKEVRIFDTTLRDGEQTPGISFTKEQKLMIARQLAKLGVASIEAGFPAVSQGEFEAVKAIAREG
LGPEIVALARANKRDIDKALDADVDAIHVFIAASDIHLKYKLRMTREEALRRAVEAVEYAKSHGVTVEFSPEDGTRADLN
YLYTMVEAVVDAGADRVDIPDTVGVMTPTRMKYLIKFILPAAKGRIVSVHCHNDFGLAVANSIAGIEAGARQAHVTVNGI
GERAGNAALEEVVAALEFLLNVRTGVNTKLLYETSLLVSKITGIPIPPNKPIVGENVFSHESGIHVHGVINNPFTYEPIQ
PEMVGQRRRIVLGKHSGRHGVEYALKTLGYPTDPDVVLRVLDEVKRLGDMGIRVTEDKLREIVEKVLEERERAKEAATS
>Mature_399_residues
MLIVEGVRIAEELENLPKEVRIFDTTLRDGEQTPGISFTKEQKLMIARQLAKLGVASIEAGFPAVSQGEFEAVKAIAREG
LGPEIVALARANKRDIDKALDADVDAIHVFIAASDIHLKYKLRMTREEALRRAVEAVEYAKSHGVTVEFSPEDGTRADLN
YLYTMVEAVVDAGADRVDIPDTVGVMTPTRMKYLIKFILPAAKGRIVSVHCHNDFGLAVANSIAGIEAGARQAHVTVNGI
GERAGNAALEEVVAALEFLLNVRTGVNTKLLYETSLLVSKITGIPIPPNKPIVGENVFSHESGIHVHGVINNPFTYEPIQ
PEMVGQRRRIVLGKHSGRHGVEYALKTLGYPTDPDVVLRVLDEVKRLGDMGIRVTEDKLREIVEKVLEERERAKEAATS

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate)

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily

Homologues:

Organism=Homo sapiens, GI62198232, Length=294, Percent_Identity=23.469387755102, Blast_Score=75, Evalue=7e-14,
Organism=Homo sapiens, GI109150422, Length=297, Percent_Identity=24.2424242424242, Blast_Score=70, Evalue=5e-12,
Organism=Homo sapiens, GI109150427, Length=297, Percent_Identity=24.2424242424242, Blast_Score=69, Evalue=5e-12,
Organism=Escherichia coli, GI1786261, Length=365, Percent_Identity=42.4657534246575, Blast_Score=276, Evalue=2e-75,
Organism=Saccharomyces cerevisiae, GI6320071, Length=367, Percent_Identity=33.2425068119891, Blast_Score=183, Evalue=3e-47,
Organism=Saccharomyces cerevisiae, GI6320019, Length=356, Percent_Identity=32.8651685393258, Blast_Score=179, Evalue=6e-46,
Organism=Saccharomyces cerevisiae, GI6324682, Length=378, Percent_Identity=27.7777777777778, Blast_Score=117, Evalue=3e-27,
Organism=Saccharomyces cerevisiae, GI6324225, Length=377, Percent_Identity=27.8514588859416, Blast_Score=107, Evalue=4e-24,
Organism=Drosophila melanogaster, GI24582381, Length=279, Percent_Identity=27.9569892473118, Blast_Score=91, Evalue=1e-18,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LEU1_IGNH4 (A8AB61)

Other databases:

- EMBL:   CP000816
- RefSeq:   YP_001435570.1
- ProteinModelPortal:   A8AB61
- SMR:   A8AB61
- STRING:   A8AB61
- GeneID:   5563127
- GenomeReviews:   CP000816_GR
- KEGG:   iho:Igni_0983
- eggNOG:   arNOG04336
- HOGENOM:   HBG701974
- OMA:   ENSFAHE
- BioCyc:   IHOS453591:IGNI_0983-MONOMER
- HAMAP:   MF_01025_A
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR011830
- InterPro:   IPR000891
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR02090

Pfam domain/function: PF00682 HMGL-like

EC number: =2.3.3.13

Molecular weight: Translated: 43688; Mature: 43688

Theoretical pI: Translated: 6.16; Mature: 6.16

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1; PS00816 AIPM_HOMOCIT_SYNTH_2; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLIVEGVRIAEELENLPKEVRIFDTTLRDGEQTPGISFTKEQKLMIARQLAKLGVASIEA
CEEECCHHHHHHHHCCCHHEEEEEHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCHHHHHC
GFPAVSQGEFEAVKAIAREGLGPEIVALARANKRDIDKALDADVDAIHVFIAASDIHLKY
CCCCCCCCHHHHHHHHHHCCCCHHEEEECCCCHHHHHHHHCCCCCEEEEEEEEECEEEEE
KLRMTREEALRRAVEAVEYAKSHGVTVEFSPEDGTRADLNYLYTMVEAVVDAGADRVDIP
EEECCHHHHHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCC
DTVGVMTPTRMKYLIKFILPAAKGRIVSVHCHNDFGLAVANSIAGIEAGARQAHVTVNGI
CCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHCCCCCCEEEEEEECC
GERAGNAALEEVVAALEFLLNVRTGVNTKLLYETSLLVSKITGIPIPPNKPIVGENVFSH
CCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHCCCCCCCCCCCCCCCCCCC
ESGIHVHGVINNPFTYEPIQPEMVGQRRRIVLGKHSGRHGVEYALKTLGYPTDPDVVLRV
CCCCEEEEEECCCCCCCCCCHHHHCCCCEEEEECCCCCHHHHHHHHHCCCCCCHHHHHHH
LDEVKRLGDMGIRVTEDKLREIVEKVLEERERAKEAATS
HHHHHHHHHCCCEECHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLIVEGVRIAEELENLPKEVRIFDTTLRDGEQTPGISFTKEQKLMIARQLAKLGVASIEA
CEEECCHHHHHHHHCCCHHEEEEEHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCHHHHHC
GFPAVSQGEFEAVKAIAREGLGPEIVALARANKRDIDKALDADVDAIHVFIAASDIHLKY
CCCCCCCCHHHHHHHHHHCCCCHHEEEECCCCHHHHHHHHCCCCCEEEEEEEEECEEEEE
KLRMTREEALRRAVEAVEYAKSHGVTVEFSPEDGTRADLNYLYTMVEAVVDAGADRVDIP
EEECCHHHHHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCC
DTVGVMTPTRMKYLIKFILPAAKGRIVSVHCHNDFGLAVANSIAGIEAGARQAHVTVNGI
CCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHCCCCCCEEEEEEECC
GERAGNAALEEVVAALEFLLNVRTGVNTKLLYETSLLVSKITGIPIPPNKPIVGENVFSH
CCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHCCCCCCCCCCCCCCCCCCC
ESGIHVHGVINNPFTYEPIQPEMVGQRRRIVLGKHSGRHGVEYALKTLGYPTDPDVVLRV
CCCCEEEEEECCCCCCCCCCHHHHCCCCEEEEECCCCCHHHHHHHHHCCCCCCHHHHHHH
LDEVKRLGDMGIRVTEDKLREIVEKVLEERERAKEAATS
HHHHHHHHHCCCEECHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA