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The map label for this gene is leuS [H]

Identifier: 156937729

GI number: 156937729

Start: 830236

End: 832773

Strand: Direct

Name: leuS [H]

Synonym: Igni_0938

Alternate gene names: 156937729

Gene position: 830236-832773 (Clockwise)

Preceding gene: 156937726

Following gene: 156937730

Centisome position: 63.99

GC content: 57.21

Gene sequence:

>2538_bases
TTGCAACAGAAGTTCTTCGTTACCGCAGCTTTCCCCTACGCCCTCCCGCTCGACGTGGACGAGCTCGTAACTAGGGTGCG
CTCCTACCTAGTAGCCGACGTGTACGCGAGGTACCACGAGGCTAGGGGGAGGAGGGTCCTATTTCCCATGGCTTTCCACT
ACAGCGGCACGCCCCTCGTTTCCTTCTACGAGAGGGTGAAGTCCCGCTCCCCGGAGGCGCTGAAGTTATTAAAGGAACTG
GGCGTCCCCGAGGACGTAAAGAGCCCTAAGGAACTTGGGGACTTCATGTCCAGAAAGCTGAAGGAGCTCTTCGATTCGTT
GAGGTTGAGGATAGATTGGGAGCACTCGTTCACCACGGAGGATCCCGGATTTAAGTCCTTTGTAAAGTGGATATACTTAA
AACTGAAGGAAAAGGGCTTAGTGGTCAAGGGCACTTACCCGGTGCCGTGGGACCCGGTAGAAGAGGTCCCGGTGAGCTCC
CACGACACTGAAGGCTTCAAGCAGATAAAGGTGGGATCCTTCTTCTTGTTAATGTTCGAGTTGAGCCCGGGCTTGTACTT
GCCGGCGGCCGCCCGGCCGGAGACCTCATTCGGTATAACTAACATATGGATCAACCCCAAGGCAGAGTACAAGATAGTGG
AGGCCGGCGGCAAGAGGATGATTATGTCGTCCAAGGCAGCTTTTAAGATTAGGTTCCAGTTGGACGAGGTGTTTGAAGTA
GGCAAGGTAGATCCTGAGGAGATCCTCGGCAAGAGGGCCGTCAACCCCATAACTGGGGAGAGCGTGCCCGTGCTGCCCTC
AAGCTCGGTGGACCCGGAGAGGGGAAGCGGCGTCGTGGCCTCGAAGCCTGCGCACGACGTGAACGACTACCGCGAGGTGC
AGAAGCTCTTGAGCAAACCCTTCGTGCTCGAGAGCTTCGACGTTGACCCGAAGGAGCTAGAGCCGAAGGCCGTGATAGAG
CTGCCCGGGTGTGAGGTCCCAGCGGCTTGCTTCGAGGACAACAGGGAGCTCGTGCTCGCGGAAAGGAGCAAGGGCAAGCT
CAAACCCGAAGCGGTCTTAAAGGCGTTGAAGGGCTCGGAGGACCAGTTCCTCAAGGGCTTGCTCTTGACCGTGTTGACCG
AGCGACCGGTCGCGGAGGTCTCGGAAGTAATAAAGAGAGCTGCAACCGTCGGGGGAGTTGCTTTAGAGTTTTACGACGTA
TTGAACGGACCGATAAGGTCTAGGTTCGGTAACGAAGTAGTGGTGAAGGTCCTCAAGGACCAGTGGTTCTTGAACTACGA
CGACGAGAAGTGGAAGAAGAAGGCGTTAGAGGTTCTTAGCCTGATGGAGGTGGTTCCCAAAGAAGTCTCGAGGTCCTTAG
TAACGGTCATAGAAAGCCTCAAGAAGAGGGCGTTCGCTCTGGCACGGGGCTTGGGCACCCCGTTGCCGTGGGACGAGAAC
TTAGTAATAGACAGCCTCAGCGACTCCACCTTGTATTACTTGTTCTACCTATTTGCAGACGTCCTCAAGGACAAAGAAGT
GAAACCCGAGGCGTGGGACTACTTGATACTGGGGGAGGGCAAGGCGGAAGACTTGAAGTACTTGAGGAAGAGGTTCTTGG
AGTGGATGCCCCTAGACATGAGGGTTGTCCACGAGGAGCTCGTGAGCAACCACGTCGCCTACATGCTGTTCCACCACGCG
GCCATCTTCGGAACCTCCAACGTTCCGAGGAAGTTGTTGGTAACTGGAGAGGTGGAGGGCGTCAGCGTAAACCCGTTTAA
GGAGGACCCGCAAGCCTTGAGGTTGTACTTGCTGACCGCGGCGAAGCCCACCTCGCCTCTGAAGTTCTCGGAAAAGGACC
TCCGCGAGGTTCAAAAGAGGATAGAGGAGATACTCAAGGAGTGGCCCTCCGGGGACAGGGAGGCCGGGGAGCTCGAGGAG
TGGCTGGAGTCGGCCTTGGCGCTGAGGGTTCAAAGGGCCGAGGCGGCGCTGGAGCAAGGGGACGTTAGGGAGGCGGCCCT
ACAAGCCCTGGGCTTCTTGAAGGACTTGAGGAGGTACTACCAGAGGTTGGAGGACAAGGGCTTGCCGCCCTCCAAGGAAG
TGAGGAGGCTGCTGAGGGAGTGGGGCTCGCTGTTGTACCCGTTCGCGCCCTCGCTGGCGGAGGCCCTAGGAGGGGTCCCC
CGGTGGCCTTCCTTGGAGCGGAACTTGGAGGTCGAGGCTAAAGAAGCATACTTCGATATGTTACTAAATGAAATTAAAAA
GGTGAAGTCGGACAAAGTCCTAGTAGAGGTGGCCCCTAGGGAGGCGACTGAGGCGCTCAGGGAAGTGGTGGAGAGCGTGG
AGGAGGGGGTCTGGGAAGAGCTGGAGGACGTCCCCCGGCAGCTGTTGGAGAGGGCCTTGAAGTTGAGCGAGGAGTGGAGG
GAGTTGTTGTACTACATAAAGGACGAATACGAGATGGCGAGCTCCCTAAAGGAGGAGTTGGAGAGGAGGACCGGCAAGGA
GGTCCACGTAAAGGTGGGAGACGCACAGCCCTTGTCCCCCAAGGTGAGGGGCCTTTGA

Upstream 100 bases:

>100_bases
TCCGGGACCTCTCCATTACGTACCTATAGAGCTCTTCAAACCCCGTCATCTTGTGATTACCCATTAATTCCCCATATAAA
CTTCTCTACCGGGTGTACGG

Downstream 100 bases:

>100_bases
GTCGCGAGGCGCTCGCGAAGAGGATGGCTAGGGCCTTCAAGGCCCTCGGCTTCTTCATATTTGAGGCTGACGAGTTGCCG
GACGTCAAGGAGGTCCTCGA

Product: leucyl-tRNA synthetase

Products: NA

Alternate protein names: Leucine--tRNA ligase; LeuRS [H]

Number of amino acids: Translated: 845; Mature: 845

Protein sequence:

>845_residues
MQQKFFVTAAFPYALPLDVDELVTRVRSYLVADVYARYHEARGRRVLFPMAFHYSGTPLVSFYERVKSRSPEALKLLKEL
GVPEDVKSPKELGDFMSRKLKELFDSLRLRIDWEHSFTTEDPGFKSFVKWIYLKLKEKGLVVKGTYPVPWDPVEEVPVSS
HDTEGFKQIKVGSFFLLMFELSPGLYLPAAARPETSFGITNIWINPKAEYKIVEAGGKRMIMSSKAAFKIRFQLDEVFEV
GKVDPEEILGKRAVNPITGESVPVLPSSSVDPERGSGVVASKPAHDVNDYREVQKLLSKPFVLESFDVDPKELEPKAVIE
LPGCEVPAACFEDNRELVLAERSKGKLKPEAVLKALKGSEDQFLKGLLLTVLTERPVAEVSEVIKRAATVGGVALEFYDV
LNGPIRSRFGNEVVVKVLKDQWFLNYDDEKWKKKALEVLSLMEVVPKEVSRSLVTVIESLKKRAFALARGLGTPLPWDEN
LVIDSLSDSTLYYLFYLFADVLKDKEVKPEAWDYLILGEGKAEDLKYLRKRFLEWMPLDMRVVHEELVSNHVAYMLFHHA
AIFGTSNVPRKLLVTGEVEGVSVNPFKEDPQALRLYLLTAAKPTSPLKFSEKDLREVQKRIEEILKEWPSGDREAGELEE
WLESALALRVQRAEAALEQGDVREAALQALGFLKDLRRYYQRLEDKGLPPSKEVRRLLREWGSLLYPFAPSLAEALGGVP
RWPSLERNLEVEAKEAYFDMLLNEIKKVKSDKVLVEVAPREATEALREVVESVEEGVWEELEDVPRQLLERALKLSEEWR
ELLYYIKDEYEMASSLKEELERRTGKEVHVKVGDAQPLSPKVRGL

Sequences:

>Translated_845_residues
MQQKFFVTAAFPYALPLDVDELVTRVRSYLVADVYARYHEARGRRVLFPMAFHYSGTPLVSFYERVKSRSPEALKLLKEL
GVPEDVKSPKELGDFMSRKLKELFDSLRLRIDWEHSFTTEDPGFKSFVKWIYLKLKEKGLVVKGTYPVPWDPVEEVPVSS
HDTEGFKQIKVGSFFLLMFELSPGLYLPAAARPETSFGITNIWINPKAEYKIVEAGGKRMIMSSKAAFKIRFQLDEVFEV
GKVDPEEILGKRAVNPITGESVPVLPSSSVDPERGSGVVASKPAHDVNDYREVQKLLSKPFVLESFDVDPKELEPKAVIE
LPGCEVPAACFEDNRELVLAERSKGKLKPEAVLKALKGSEDQFLKGLLLTVLTERPVAEVSEVIKRAATVGGVALEFYDV
LNGPIRSRFGNEVVVKVLKDQWFLNYDDEKWKKKALEVLSLMEVVPKEVSRSLVTVIESLKKRAFALARGLGTPLPWDEN
LVIDSLSDSTLYYLFYLFADVLKDKEVKPEAWDYLILGEGKAEDLKYLRKRFLEWMPLDMRVVHEELVSNHVAYMLFHHA
AIFGTSNVPRKLLVTGEVEGVSVNPFKEDPQALRLYLLTAAKPTSPLKFSEKDLREVQKRIEEILKEWPSGDREAGELEE
WLESALALRVQRAEAALEQGDVREAALQALGFLKDLRRYYQRLEDKGLPPSKEVRRLLREWGSLLYPFAPSLAEALGGVP
RWPSLERNLEVEAKEAYFDMLLNEIKKVKSDKVLVEVAPREATEALREVVESVEEGVWEELEDVPRQLLERALKLSEEWR
ELLYYIKDEYEMASSLKEELERRTGKEVHVKVGDAQPLSPKVRGL
>Mature_845_residues
MQQKFFVTAAFPYALPLDVDELVTRVRSYLVADVYARYHEARGRRVLFPMAFHYSGTPLVSFYERVKSRSPEALKLLKEL
GVPEDVKSPKELGDFMSRKLKELFDSLRLRIDWEHSFTTEDPGFKSFVKWIYLKLKEKGLVVKGTYPVPWDPVEEVPVSS
HDTEGFKQIKVGSFFLLMFELSPGLYLPAAARPETSFGITNIWINPKAEYKIVEAGGKRMIMSSKAAFKIRFQLDEVFEV
GKVDPEEILGKRAVNPITGESVPVLPSSSVDPERGSGVVASKPAHDVNDYREVQKLLSKPFVLESFDVDPKELEPKAVIE
LPGCEVPAACFEDNRELVLAERSKGKLKPEAVLKALKGSEDQFLKGLLLTVLTERPVAEVSEVIKRAATVGGVALEFYDV
LNGPIRSRFGNEVVVKVLKDQWFLNYDDEKWKKKALEVLSLMEVVPKEVSRSLVTVIESLKKRAFALARGLGTPLPWDEN
LVIDSLSDSTLYYLFYLFADVLKDKEVKPEAWDYLILGEGKAEDLKYLRKRFLEWMPLDMRVVHEELVSNHVAYMLFHHA
AIFGTSNVPRKLLVTGEVEGVSVNPFKEDPQALRLYLLTAAKPTSPLKFSEKDLREVQKRIEEILKEWPSGDREAGELEE
WLESALALRVQRAEAALEQGDVREAALQALGFLKDLRRYYQRLEDKGLPPSKEVRRLLREWGSLLYPFAPSLAEALGGVP
RWPSLERNLEVEAKEAYFDMLLNEIKKVKSDKVLVEVAPREATEALREVVESVEEGVWEELEDVPRQLLERALKLSEEWR
ELLYYIKDEYEMASSLKEELERRTGKEVHVKVGDAQPLSPKVRGL

Specific function: Unknown

COG id: COG0495

COG function: function code J; Leucyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI108773810, Length=505, Percent_Identity=30.0990099009901, Blast_Score=199, Evalue=1e-50,
Organism=Escherichia coli, GI1790708, Length=297, Percent_Identity=24.9158249158249, Blast_Score=84, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI17554638, Length=527, Percent_Identity=29.6015180265655, Blast_Score=201, Evalue=2e-51,
Organism=Saccharomyces cerevisiae, GI6325097, Length=855, Percent_Identity=23.3918128654971, Blast_Score=182, Evalue=2e-46,
Organism=Drosophila melanogaster, GI28574733, Length=708, Percent_Identity=25.2824858757062, Blast_Score=195, Evalue=9e-50,

Paralogues:

None

Copy number: 120 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015413
- InterPro:   IPR002300
- InterPro:   IPR020791
- InterPro:   IPR004493
- InterPro:   IPR014729
- InterPro:   IPR009080
- InterPro:   IPR013155
- InterPro:   IPR009008 [H]

Pfam domain/function: PF08264 Anticodon_1; PF00133 tRNA-synt_1; PF09334 tRNA-synt_1g [H]

EC number: =6.1.1.4 [H]

Molecular weight: Translated: 96259; Mature: 96259

Theoretical pI: Translated: 5.35; Mature: 5.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQQKFFVTAAFPYALPLDVDELVTRVRSYLVADVYARYHEARGRRVLFPMAFHYSGTPLV
CCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCHHH
SFYERVKSRSPEALKLLKELGVPEDVKSPKELGDFMSRKLKELFDSLRLRIDWEHSFTTE
HHHHHHHCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCC
DPGFKSFVKWIYLKLKEKGLVVKGTYPVPWDPVEEVPVSSHDTEGFKQIKVGSFFLLMFE
CCCHHHHHHHHHHHHHCCCEEEECCCCCCCCCHHHCCCCCCCCCCHHHEEHHHHEEEEEE
LSPGLYLPAAARPETSFGITNIWINPKAEYKIVEAGGKRMIMSSKAAFKIRFQLDEVFEV
CCCCCCCCCCCCCCCCCCEEEEEECCCCCEEEEECCCCEEEEECCCEEEEEEEHHHHHHH
GKVDPEEILGKRAVNPITGESVPVLPSSSVDPERGSGVVASKPAHDVNDYREVQKLLSKP
CCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCC
FVLESFDVDPKELEPKAVIELPGCEVPAACFEDNRELVLAERSKGKLKPEAVLKALKGSE
CEEECCCCCHHHCCCCEEEECCCCCCCHHHHCCCCEEEEEECCCCCCCHHHHHHHHCCCH
DQFLKGLLLTVLTERPVAEVSEVIKRAATVGGVALEFYDVLNGPIRSRFGNEVVVKVLKD
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCHHHHHHHHHHCCHHHHHCCHHHHHHHHHH
QWFLNYDDEKWKKKALEVLSLMEVVPKEVSRSLVTVIESLKKRAFALARGLGTPLPWDEN
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
LVIDSLSDSTLYYLFYLFADVLKDKEVKPEAWDYLILGEGKAEDLKYLRKRFLEWMPLDM
EEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHCCCHH
RVVHEELVSNHVAYMLFHHAAIFGTSNVPRKLLVTGEVEGVSVNPFKEDPQALRLYLLTA
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCHHHHEEHHEEC
AKPTSPLKFSEKDLREVQKRIEEILKEWPSGDREAGELEEWLESALALRVQRAEAALEQG
CCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
DVREAALQALGFLKDLRRYYQRLEDKGLPPSKEVRRLLREWGSLLYPFAPSLAEALGGVP
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHCCCC
RWPSLERNLEVEAKEAYFDMLLNEIKKVKSDKVLVEVAPREATEALREVVESVEEGVWEE
CCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHH
LEDVPRQLLERALKLSEEWRELLYYIKDEYEMASSLKEELERRTGKEVHVKVGDAQPLSP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCC
KVRGL
CCCCC
>Mature Secondary Structure
MQQKFFVTAAFPYALPLDVDELVTRVRSYLVADVYARYHEARGRRVLFPMAFHYSGTPLV
CCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCHHH
SFYERVKSRSPEALKLLKELGVPEDVKSPKELGDFMSRKLKELFDSLRLRIDWEHSFTTE
HHHHHHHCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCC
DPGFKSFVKWIYLKLKEKGLVVKGTYPVPWDPVEEVPVSSHDTEGFKQIKVGSFFLLMFE
CCCHHHHHHHHHHHHHCCCEEEECCCCCCCCCHHHCCCCCCCCCCHHHEEHHHHEEEEEE
LSPGLYLPAAARPETSFGITNIWINPKAEYKIVEAGGKRMIMSSKAAFKIRFQLDEVFEV
CCCCCCCCCCCCCCCCCCEEEEEECCCCCEEEEECCCCEEEEECCCEEEEEEEHHHHHHH
GKVDPEEILGKRAVNPITGESVPVLPSSSVDPERGSGVVASKPAHDVNDYREVQKLLSKP
CCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCC
FVLESFDVDPKELEPKAVIELPGCEVPAACFEDNRELVLAERSKGKLKPEAVLKALKGSE
CEEECCCCCHHHCCCCEEEECCCCCCCHHHHCCCCEEEEEECCCCCCCHHHHHHHHCCCH
DQFLKGLLLTVLTERPVAEVSEVIKRAATVGGVALEFYDVLNGPIRSRFGNEVVVKVLKD
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCHHHHHHHHHHCCHHHHHCCHHHHHHHHHH
QWFLNYDDEKWKKKALEVLSLMEVVPKEVSRSLVTVIESLKKRAFALARGLGTPLPWDEN
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
LVIDSLSDSTLYYLFYLFADVLKDKEVKPEAWDYLILGEGKAEDLKYLRKRFLEWMPLDM
EEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHCCCHH
RVVHEELVSNHVAYMLFHHAAIFGTSNVPRKLLVTGEVEGVSVNPFKEDPQALRLYLLTA
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCHHHHEEHHEEC
AKPTSPLKFSEKDLREVQKRIEEILKEWPSGDREAGELEEWLESALALRVQRAEAALEQG
CCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
DVREAALQALGFLKDLRRYYQRLEDKGLPPSKEVRRLLREWGSLLYPFAPSLAEALGGVP
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHCCCC
RWPSLERNLEVEAKEAYFDMLLNEIKKVKSDKVLVEVAPREATEALREVVESVEEGVWEE
CCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHH
LEDVPRQLLERALKLSEEWRELLYYIKDEYEMASSLKEELERRTGKEVHVKVGDAQPLSP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCC
KVRGL
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA