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The map label for this gene is thiI [H]

Identifier: 156937695

GI number: 156937695

Start: 803810

End: 804601

Strand: Reverse

Name: thiI [H]

Synonym: Igni_0904

Alternate gene names: 156937695

Gene position: 804601-803810 (Counterclockwise)

Preceding gene: 156937696

Following gene: 156937694

Centisome position: 62.01

GC content: 64.02

Gene sequence:

>792_bases
ATGTCTCCCGATACCGTCCTTCTGATAGCCTATTCCGAAATAGCCCTCAAGGGAAAGAAGAGAGGTTTTATGGAGAGGGC
GCTGGCCTCTTCGATAAGGCGCTGGCACGAGAGGAGGGGGCTGGAGGTAAGGCTCCAGCACGAGGGCGGGAGGTTAGTGG
TGGAGGGGGCGTTCAGCGCGGAAGTCGCTAGGGTGCCCGGGGTCCACCACGTGGCCCGCGCGTTCGCCCTCGAGAAGGCG
CCTCCGGAGGAGCTAGTGGAGGCCATAGCCGAAGTGGTGAAGGCGGAGCCTCCGGTGGCGGTGAGGGTCAAGAGGGGGGA
CAAGAGGTACCCATTGACCAGCGTGGAGCTCGCCGCGAAGGTGGGCGAAGCCTTGGGCCTTCCGGTGGACTTGAAGAGCC
CTAGGACGGCGATCTTCGTAGAAATAAGGAAGAGGGTTTACGTCTACACCTCGAGGGACGTAAGGGAAGGGGTAGGGGGG
CTTCCCTACGGCGTGGAGGGGGAGGTCGGAGTGAGCTACCGGGACCCCCTCAAGGGCCTCTTGGCCTCCGCGCTGTTGGC
CCGAAGGGGGGCTAGGGTCTTCCCCCACGAGCCCTACCCGGGGTTGGAGGCGCTCGCGGAGGCCCTCCCCGAGCCCCTCA
TTATTAAGCGCTCTCCCCCGCCCGTCGCCGGAAGGGACCTCTTCCCGGGGGAGCCTTGTGTCCCTCAGAGGACCTTGTCC
AAGGTTAAGCTCATGTTAGGGGGTGAGGGCGTTGAAGGTATTGATCGCTTCGGATGCCCACGTGCCGGATAG

Upstream 100 bases:

>100_bases
TGGCCAAACTCATCGCAGAGGACAAGGCTACCCCGGAAGACCTGAGCTACCTAATGTGTAAGACTTGTAGGACGCTCATG
CGCAAGTGGCAGAAGGCGAC

Downstream 100 bases:

>100_bases
GGAGAGGGAAATACCAAAGGAGATAAGGGAGTTCCTGGCCTCCGAGGCCCCCTTCGACCTAGTCTTGTACGCTGGGGACT
TGACAGGGCCGGAGGTGCTG

Product: THUMP domain-containing protein

Products: NA

Alternate protein names: Sulfur carrier protein ThiS sulfurtransferase; Thiamine biosynthesis protein thiI; tRNA 4-thiouridine synthase [H]

Number of amino acids: Translated: 263; Mature: 262

Protein sequence:

>263_residues
MSPDTVLLIAYSEIALKGKKRGFMERALASSIRRWHERRGLEVRLQHEGGRLVVEGAFSAEVARVPGVHHVARAFALEKA
PPEELVEAIAEVVKAEPPVAVRVKRGDKRYPLTSVELAAKVGEALGLPVDLKSPRTAIFVEIRKRVYVYTSRDVREGVGG
LPYGVEGEVGVSYRDPLKGLLASALLARRGARVFPHEPYPGLEALAEALPEPLIIKRSPPPVAGRDLFPGEPCVPQRTLS
KVKLMLGGEGVEGIDRFGCPRAG

Sequences:

>Translated_263_residues
MSPDTVLLIAYSEIALKGKKRGFMERALASSIRRWHERRGLEVRLQHEGGRLVVEGAFSAEVARVPGVHHVARAFALEKA
PPEELVEAIAEVVKAEPPVAVRVKRGDKRYPLTSVELAAKVGEALGLPVDLKSPRTAIFVEIRKRVYVYTSRDVREGVGG
LPYGVEGEVGVSYRDPLKGLLASALLARRGARVFPHEPYPGLEALAEALPEPLIIKRSPPPVAGRDLFPGEPCVPQRTLS
KVKLMLGGEGVEGIDRFGCPRAG
>Mature_262_residues
SPDTVLLIAYSEIALKGKKRGFMERALASSIRRWHERRGLEVRLQHEGGRLVVEGAFSAEVARVPGVHHVARAFALEKAP
PEELVEAIAEVVKAEPPVAVRVKRGDKRYPLTSVELAAKVGEALGLPVDLKSPRTAIFVEIRKRVYVYTSRDVREGVGGL
PYGVEGEVGVSYRDPLKGLLASALLARRGARVFPHEPYPGLEALAEALPEPLIIKRSPPPVAGRDLFPGEPCVPQRTLSK
VKLMLGGEGVEGIDRFGCPRAG

Specific function: Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor. Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. Thi

COG id: COG0301

COG function: function code H; Thiamine biosynthesis ATP pyrophosphatase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 THUMP domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014729
- InterPro:   IPR003720
- InterPro:   IPR020536
- InterPro:   IPR004114 [H]

Pfam domain/function: PF02568 ThiI; PF02926 THUMP [H]

EC number: =2.8.1.4 [H]

Molecular weight: Translated: 28569; Mature: 28437

Theoretical pI: Translated: 10.00; Mature: 10.00

Prosite motif: PS51165 THUMP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPDTVLLIAYSEIALKGKKRGFMERALASSIRRWHERRGLEVRLQHEGGRLVVEGAFSA
CCCCEEEEEEEHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCEEEEEECCCH
EVARVPGVHHVARAFALEKAPPEELVEAIAEVVKAEPPVAVRVKRGDKRYPLTSVELAAK
HHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHH
VGEALGLPVDLKSPRTAIFVEIRKRVYVYTSRDVREGVGGLPYGVEGEVGVSYRDPLKGL
HHHHHCCCEECCCCCCEEEEEEECEEEEEECCHHHHCCCCCCCCCCCCCCCCHHHHHHHH
LASALLARRGARVFPHEPYPGLEALAEALPEPLIIKRSPPPVAGRDLFPGEPCVPQRTLS
HHHHHHHHCCCCCCCCCCCCCHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCCCHHHHH
KVKLMLGGEGVEGIDRFGCPRAG
HHHHHCCCCCCCHHHHCCCCCCC
>Mature Secondary Structure 
SPDTVLLIAYSEIALKGKKRGFMERALASSIRRWHERRGLEVRLQHEGGRLVVEGAFSA
CCCEEEEEEEHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCEEEEEECCCH
EVARVPGVHHVARAFALEKAPPEELVEAIAEVVKAEPPVAVRVKRGDKRYPLTSVELAAK
HHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHH
VGEALGLPVDLKSPRTAIFVEIRKRVYVYTSRDVREGVGGLPYGVEGEVGVSYRDPLKGL
HHHHHCCCEECCCCCCEEEEEEECEEEEEECCHHHHCCCCCCCCCCCCCCCCHHHHHHHH
LASALLARRGARVFPHEPYPGLEALAEALPEPLIIKRSPPPVAGRDLFPGEPCVPQRTLS
HHHHHHHHCCCCCCCCCCCCCHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCCCHHHHH
KVKLMLGGEGVEGIDRFGCPRAG
HHHHHCCCCCCCHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9389475 [H]