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The map label for this gene is purF [H]

Identifier: 156937693

GI number: 156937693

Start: 801971

End: 803269

Strand: Reverse

Name: purF [H]

Synonym: Igni_0902

Alternate gene names: 156937693

Gene position: 803269-801971 (Counterclockwise)

Preceding gene: 156937694

Following gene: 156937691

Centisome position: 61.91

GC content: 61.2

Gene sequence:

>1299_bases
TTGTGTGGCGTGGCCGCGGCCCCGTGGGCCGACGAGGTCTACGTGCTCCTAGAGGGCCTTCAGCACAGAGGCCAAGAGAG
CGCCGGCGTCGCTTGGGTCGAGGACGGGGAGATCAAGTTCTCGGGAGGGATGGGGCTAGTAAAAGACGCGATAAAGGAGG
CCCCGCACAAGGGGCCCGCGATAGGCCACGTGAGGTACTCCACGTCCGGCGGCTACGCCAGGGTTCAGCCTGTCGTAACG
AAGAAATTGGCCCTTGCCTTCAATGGTAATATAATAAACTTCAAGTTCCTCGAGCCCAGCTCCCGGTGGGACGCGGAAGC
CCTAATCAAATCCATCATTAACGAGAAGACTAAGGGGTTGGACCTCTTCGAGGCGGCCAGGAGGGTGCTAGAGAGGGCGA
AGGGGAGCTACAGCTTGGTCGCCCTCGCCGCCGACGGGAGGGTGTTGGTCGCGAGGGACCCTTGGGGCTTCAGGCCGCTG
GCCTACCGCTGGCCCCATGTGGCCTCCGAGACCGCGGCGCTGGAGGACATCGGCATGACGTGGGAGGAATTGGAGCCCAA
CAAGCTGGTCATGCTCGAGGAGGGGGTCCCCACCAAGGAAGCCAAAATAAGTTACGACAGGAGGAAGGCCTACTGCGCCT
TCGAGTACGTGTACTTCCAGAGGCCGGAGAGCTACTTCAACGGGGTTAACGTCCACGTGTCTAGGGTTAGGATGGGAATG
ATACTAGCCGAGGAGAAGCCCGCGGACGCGGACGTGGTCTTGCCGGTCCCGGACTCGGGGAGGAGCGCGGCGATAGGCTA
CTCCAGGCGCTCGGGAATCCCCTTGGACGAGGGCTTGGTGAAGAATAGGTACTTGGGGAGGAGCTTCATAATGCCCCCAG
GCCTAAGGGAGGTAATAGCCATGAAGAAGTACGGGGTAGTTAAGGAGGTGGTGGAGGGGAAGAGGGTAGTGGTGGTGGAC
GACAGCATAGTGAGGGGGACCACCATGAAGAGGATAGTGAAACTCTTAAAGGAGAAGGGGGCCGAGGAGGTGCACGTGAG
GATAGCCTCTCCCCCCGTGAGGGCCCCTTGTTACATGGGGATAGACTTCCCCTCTAGAGAGGAGCTGGTCGCGGCGGAGA
GGGGCGAGGAGGAGCTGAGCGAGCTCTGGGGCGCGGACAGCGTGGGCTACTTGAGCGTGGAAGGGCTGAGGAGGGCCATA
GGCCTCGAGGAGCTCTGCGTGGCCTGCTTCACCGACGTGTACCCCTTCCCCATCTCCGAAGAAGAGGTGAGGAGCATGCT
CAAAGCTCACAAGGCTTGA

Upstream 100 bases:

>100_bases
AGGTGGACCTCTACGCCCTCAAGGGCGGGAGGCTCGAGAGGAGCTCTTACAGTTTAAGGATATAATTCGTGCGGGCGGCC
CTCAGTAGGGTATAAATGTA

Downstream 100 bases:

>100_bases
AAGGAGACGAACTTCTCCGCCGCCACTTCGGTCATCTTACCCTTGACCCATATCACCCTCGCTATGGCCCCTGTGACGCC
CTTGCTCGCCTCGACCTTCA

Product: amidophosphoribosyltransferase

Products: NA

Alternate protein names: ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase [H]

Number of amino acids: Translated: 432; Mature: 432

Protein sequence:

>432_residues
MCGVAAAPWADEVYVLLEGLQHRGQESAGVAWVEDGEIKFSGGMGLVKDAIKEAPHKGPAIGHVRYSTSGGYARVQPVVT
KKLALAFNGNIINFKFLEPSSRWDAEALIKSIINEKTKGLDLFEAARRVLERAKGSYSLVALAADGRVLVARDPWGFRPL
AYRWPHVASETAALEDIGMTWEELEPNKLVMLEEGVPTKEAKISYDRRKAYCAFEYVYFQRPESYFNGVNVHVSRVRMGM
ILAEEKPADADVVLPVPDSGRSAAIGYSRRSGIPLDEGLVKNRYLGRSFIMPPGLREVIAMKKYGVVKEVVEGKRVVVVD
DSIVRGTTMKRIVKLLKEKGAEEVHVRIASPPVRAPCYMGIDFPSREELVAAERGEEELSELWGADSVGYLSVEGLRRAI
GLEELCVACFTDVYPFPISEEEVRSMLKAHKA

Sequences:

>Translated_432_residues
MCGVAAAPWADEVYVLLEGLQHRGQESAGVAWVEDGEIKFSGGMGLVKDAIKEAPHKGPAIGHVRYSTSGGYARVQPVVT
KKLALAFNGNIINFKFLEPSSRWDAEALIKSIINEKTKGLDLFEAARRVLERAKGSYSLVALAADGRVLVARDPWGFRPL
AYRWPHVASETAALEDIGMTWEELEPNKLVMLEEGVPTKEAKISYDRRKAYCAFEYVYFQRPESYFNGVNVHVSRVRMGM
ILAEEKPADADVVLPVPDSGRSAAIGYSRRSGIPLDEGLVKNRYLGRSFIMPPGLREVIAMKKYGVVKEVVEGKRVVVVD
DSIVRGTTMKRIVKLLKEKGAEEVHVRIASPPVRAPCYMGIDFPSREELVAAERGEEELSELWGADSVGYLSVEGLRRAI
GLEELCVACFTDVYPFPISEEEVRSMLKAHKA
>Mature_432_residues
MCGVAAAPWADEVYVLLEGLQHRGQESAGVAWVEDGEIKFSGGMGLVKDAIKEAPHKGPAIGHVRYSTSGGYARVQPVVT
KKLALAFNGNIINFKFLEPSSRWDAEALIKSIINEKTKGLDLFEAARRVLERAKGSYSLVALAADGRVLVARDPWGFRPL
AYRWPHVASETAALEDIGMTWEELEPNKLVMLEEGVPTKEAKISYDRRKAYCAFEYVYFQRPESYFNGVNVHVSRVRMGM
ILAEEKPADADVVLPVPDSGRSAAIGYSRRSGIPLDEGLVKNRYLGRSFIMPPGLREVIAMKKYGVVKEVVEGKRVVVVD
DSIVRGTTMKRIVKLLKEKGAEEVHVRIASPPVRAPCYMGIDFPSREELVAAERGEEELSELWGADSVGYLSVEGLRRAI
GLEELCVACFTDVYPFPISEEEVRSMLKAHKA

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0034

COG function: function code F; Glutamine phosphoribosylpyrophosphate amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI29570798, Length=503, Percent_Identity=35.7852882703777, Blast_Score=255, Evalue=4e-68,
Organism=Escherichia coli, GI1788651, Length=467, Percent_Identity=31.6916488222698, Blast_Score=204, Evalue=1e-53,
Organism=Caenorhabditis elegans, GI17554892, Length=480, Percent_Identity=37.2916666666667, Blast_Score=253, Evalue=2e-67,
Organism=Saccharomyces cerevisiae, GI6323958, Length=450, Percent_Identity=33.7777777777778, Blast_Score=211, Evalue=3e-55,
Organism=Drosophila melanogaster, GI24659598, Length=489, Percent_Identity=39.0593047034765, Blast_Score=278, Evalue=5e-75,
Organism=Drosophila melanogaster, GI24659604, Length=464, Percent_Identity=39.8706896551724, Blast_Score=277, Evalue=1e-74,
Organism=Drosophila melanogaster, GI28573187, Length=492, Percent_Identity=38.4146341463415, Blast_Score=268, Evalue=6e-72,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00310 GATase_2; PF00156 Pribosyltran [H]

EC number: =2.4.2.14 [H]

Molecular weight: Translated: 47816; Mature: 47816

Theoretical pI: Translated: 6.88; Mature: 6.88

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGVAAAPWADEVYVLLEGLQHRGQESAGVAWVEDGEIKFSGGMGLVKDAIKEAPHKGPA
CCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEECCEEEECCCCHHHHHHHHHCCCCCCC
IGHVRYSTSGGYARVQPVVTKKLALAFNGNIINFKFLEPSSRWDAEALIKSIINEKTKGL
EEEEEEECCCCCEEECHHHHHEEEEEECCCEEEEEEECCCCCCCHHHHHHHHHHHHCCCC
DLFEAARRVLERAKGSYSLVALAADGRVLVARDPWGFRPLAYRWPHVASETAALEDIGMT
HHHHHHHHHHHHCCCCEEEEEEECCCEEEEEECCCCCCCHHCCCCCCCCHHHHHHHHCCC
WEELEPNKLVMLEEGVPTKEAKISYDRRKAYCAFEYVYFQRPESYFNGVNVHVSRVRMGM
HHHCCCCEEEEEECCCCCCHHHCCHHHHHHEEEEEEEEEECCHHHHCCCEEEEEHEEEEE
ILAEEKPADADVVLPVPDSGRSAAIGYSRRSGIPLDEGLVKNRYLGRSFIMPPGLREVIA
EEECCCCCCCEEEEECCCCCCCEEECCCCCCCCCCCCCHHHHHCCCCCCCCCCCHHHHHH
MKKYGVVKEVVEGKRVVVVDDSIVRGTTMKRIVKLLKEKGAEEVHVRIASPPVRAPCYMG
HHHHHHHHHHHCCCEEEEECCCHHCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEEC
IDFPSREELVAAERGEEELSELWGADSVGYLSVEGLRRAIGLEELCVACFTDVYPFPISE
CCCCCHHHHHHHHCCHHHHHHHHCCCCCCEEEHHHHHHHHCHHHHHHHHHHHCCCCCCCH
EEVRSMLKAHKA
HHHHHHHHHHCC
>Mature Secondary Structure
MCGVAAAPWADEVYVLLEGLQHRGQESAGVAWVEDGEIKFSGGMGLVKDAIKEAPHKGPA
CCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEECCEEEECCCCHHHHHHHHHCCCCCCC
IGHVRYSTSGGYARVQPVVTKKLALAFNGNIINFKFLEPSSRWDAEALIKSIINEKTKGL
EEEEEEECCCCCEEECHHHHHEEEEEECCCEEEEEEECCCCCCCHHHHHHHHHHHHCCCC
DLFEAARRVLERAKGSYSLVALAADGRVLVARDPWGFRPLAYRWPHVASETAALEDIGMT
HHHHHHHHHHHHCCCCEEEEEEECCCEEEEEECCCCCCCHHCCCCCCCCHHHHHHHHCCC
WEELEPNKLVMLEEGVPTKEAKISYDRRKAYCAFEYVYFQRPESYFNGVNVHVSRVRMGM
HHHCCCCEEEEEECCCCCCHHHCCHHHHHHEEEEEEEEEECCHHHHCCCEEEEEHEEEEE
ILAEEKPADADVVLPVPDSGRSAAIGYSRRSGIPLDEGLVKNRYLGRSFIMPPGLREVIA
EEECCCCCCCEEEEECCCCCCCEEECCCCCCCCCCCCCHHHHHCCCCCCCCCCCHHHHHH
MKKYGVVKEVVEGKRVVVVDDSIVRGTTMKRIVKLLKEKGAEEVHVRIASPPVRAPCYMG
HHHHHHHHHHHCCCEEEEECCCHHCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEEC
IDFPSREELVAAERGEEELSELWGADSVGYLSVEGLRRAIGLEELCVACFTDVYPFPISE
CCCCCHHHHHHHHCCHHHHHHHHCCCCCCEEEHHHHHHHHCHHHHHHHHHHHCCCCCCCH
EEVRSMLKAHKA
HHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9679194 [H]