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The map label for this gene is pcm

Identifier: 156937670

GI number: 156937670

Start: 778559

End: 779194

Strand: Reverse

Name: pcm

Synonym: Igni_0877

Alternate gene names: 156937670

Gene position: 779194-778559 (Counterclockwise)

Preceding gene: 156937676

Following gene: 156937669

Centisome position: 60.05

GC content: 57.39

Gene sequence:

>636_bases
TTGGAGGAGAAGAAGAGGGAATTGATAAGGAACTTGATCGCTGAAGGTTACATAAAGAGGAAGGAAGTAGCCGAAGCGAT
GATGAAGGTGCCCAGGGAGCTGTTCGTTCCGGAGGAGCTCCGACACATGGCCTACGAGGACACCCCCCTTCCGATAGGGG
CGGGACAGACGATAAGTGCGCCCCACATGGTCGCTTACATGGTCGAGGCTGCCGAGCTGCGGAGGGGCGACAAGGTCCTA
GAGGTGGGCACGGGTTCTGGCTACCACGCGGCCGTAATGGCAGAGCTGGTGGGGCCGGAGGGCCACGTTTATACTATAGA
GAGGATCCCCGAGCTCGCCGAGCGCGCGAGGGAGAGGCTAAAGGCGTTGGGATACAACAACGTCACCGTGCTGGTGGGCG
ACGGAAGCAAGGGCTACCCGCCCGCCGCCCCTTACGATAAGATAATAGTAACTGCCGCGGCGAAGAGGGTTCCAGAGGCC
CTCTTGAAGCAGCTCAAGGTGGGGGGAATAATGGTGATCCCAGTGGAGGAGGAGCCGGGATATCAAGTACTCTACAAGAT
AATCAAGACTCCGGAGGGATATGTAATAAAGAAGCTGCTGCCTGTAGCCTTCGTACCTTTAATCGAGGAGGAGTAG

Upstream 100 bases:

>100_bases
GGGGTGCGGAGGGCAAGGATATGCTCAAACTGTGACACCTACCCTCGGACGCTCCACATCTCTTAAGTAACTACTTCGAA
AGAGTTCCTCGGATGAGGCC

Downstream 100 bases:

>100_bases
TCCCCGGAGAGGACGCGGGGGTTGTACGCGTCGCTGAAGCTGAGAGAGAAAGTAGAGAGGGCGATCGCGTACCTCTCGGC
GAAGAATCCCGGGCTCGGCG

Product: protein-L-isoaspartate O-methyltransferase

Products: NA

Alternate protein names: L-isoaspartyl protein carboxyl methyltransferase; Protein L-isoaspartyl methyltransferase; Protein-beta-aspartate methyltransferase; PIMT

Number of amino acids: Translated: 211; Mature: 211

Protein sequence:

>211_residues
MEEKKRELIRNLIAEGYIKRKEVAEAMMKVPRELFVPEELRHMAYEDTPLPIGAGQTISAPHMVAYMVEAAELRRGDKVL
EVGTGSGYHAAVMAELVGPEGHVYTIERIPELAERARERLKALGYNNVTVLVGDGSKGYPPAAPYDKIIVTAAAKRVPEA
LLKQLKVGGIMVIPVEEEPGYQVLYKIIKTPEGYVIKKLLPVAFVPLIEEE

Sequences:

>Translated_211_residues
MEEKKRELIRNLIAEGYIKRKEVAEAMMKVPRELFVPEELRHMAYEDTPLPIGAGQTISAPHMVAYMVEAAELRRGDKVL
EVGTGSGYHAAVMAELVGPEGHVYTIERIPELAERARERLKALGYNNVTVLVGDGSKGYPPAAPYDKIIVTAAAKRVPEA
LLKQLKVGGIMVIPVEEEPGYQVLYKIIKTPEGYVIKKLLPVAFVPLIEEE
>Mature_211_residues
MEEKKRELIRNLIAEGYIKRKEVAEAMMKVPRELFVPEELRHMAYEDTPLPIGAGQTISAPHMVAYMVEAAELRRGDKVL
EVGTGSGYHAAVMAELVGPEGHVYTIERIPELAERARERLKALGYNNVTVLVGDGSKGYPPAAPYDKIIVTAAAKRVPEA
LLKQLKVGGIMVIPVEEEPGYQVLYKIIKTPEGYVIKKLLPVAFVPLIEEE

Specific function: Catalyzes the methyl esterification of L-isoaspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins

COG id: COG2518

COG function: function code O; Protein-L-isoaspartate carboxylmethyltransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. L- isoaspartyl/D-aspartyl protein methyltransferase family

Homologues:

Organism=Homo sapiens, GI226530908, Length=213, Percent_Identity=40.3755868544601, Blast_Score=130, Evalue=6e-31,
Organism=Homo sapiens, GI157388995, Length=219, Percent_Identity=30.1369863013699, Blast_Score=97, Evalue=1e-20,
Organism=Homo sapiens, GI190360566, Length=219, Percent_Identity=29.2237442922374, Blast_Score=88, Evalue=5e-18,
Organism=Homo sapiens, GI157388997, Length=191, Percent_Identity=28.2722513089005, Blast_Score=84, Evalue=8e-17,
Organism=Escherichia coli, GI1789100, Length=204, Percent_Identity=46.078431372549, Blast_Score=166, Evalue=1e-42,
Organism=Caenorhabditis elegans, GI71983477, Length=215, Percent_Identity=39.5348837209302, Blast_Score=125, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI193207222, Length=211, Percent_Identity=38.3886255924171, Blast_Score=113, Evalue=6e-26,
Organism=Drosophila melanogaster, GI17981723, Length=219, Percent_Identity=36.986301369863, Blast_Score=126, Evalue=1e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PIMT_IGNH4 (A8AAV7)

Other databases:

- EMBL:   CP000816
- RefSeq:   YP_001435466.1
- ProteinModelPortal:   A8AAV7
- SMR:   A8AAV7
- STRING:   A8AAV7
- GeneID:   5562192
- GenomeReviews:   CP000816_GR
- KEGG:   iho:Igni_0877
- eggNOG:   arNOG08853
- HOGENOM:   HBG699907
- OMA:   GYHAAVV
- BioCyc:   IHOS453591:IGNI_0877-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00090
- InterPro:   IPR000682
- PANTHER:   PTHR11579
- TIGRFAMs:   TIGR00080

Pfam domain/function: PF01135 PCMT

EC number: =2.1.1.77

Molecular weight: Translated: 23389; Mature: 23389

Theoretical pI: Translated: 6.16; Mature: 6.16

Prosite motif: PS01279 PCMT

Important sites: ACT_SITE 59-59

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEEKKRELIRNLIAEGYIKRKEVAEAMMKVPRELFVPEELRHMAYEDTPLPIGAGQTISA
CCHHHHHHHHHHHHHCCHHHHHHHHHHHHCHHHCCCCHHHHHHHCCCCCCCCCCCCCCCC
PHMVAYMVEAAELRRGDKVLEVGTGSGYHAAVMAELVGPEGHVYTIERIPELAERARERL
HHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHH
KALGYNNVTVLVGDGSKGYPPAAPYDKIIVTAAAKRVPEALLKQLKVGGIMVIPVEEEPG
HHCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCC
YQVLYKIIKTPEGYVIKKLLPVAFVPLIEEE
HHHHHHHHHCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MEEKKRELIRNLIAEGYIKRKEVAEAMMKVPRELFVPEELRHMAYEDTPLPIGAGQTISA
CCHHHHHHHHHHHHHCCHHHHHHHHHHHHCHHHCCCCHHHHHHHCCCCCCCCCCCCCCCC
PHMVAYMVEAAELRRGDKVLEVGTGSGYHAAVMAELVGPEGHVYTIERIPELAERARERL
HHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHH
KALGYNNVTVLVGDGSKGYPPAAPYDKIIVTAAAKRVPEALLKQLKVGGIMVIPVEEEPG
HHCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCC
YQVLYKIIKTPEGYVIKKLLPVAFVPLIEEE
HHHHHHHHHCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA