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The map label for this gene is cofH [H]

Identifier: 156937661

GI number: 156937661

Start: 767559

End: 768653

Strand: Reverse

Name: cofH [H]

Synonym: Igni_0868

Alternate gene names: 156937661

Gene position: 768653-767559 (Counterclockwise)

Preceding gene: 156937662

Following gene: 156937660

Centisome position: 59.24

GC content: 56.44

Gene sequence:

>1095_bases
GTGAGGACGTGGGAGAGGAGCGACCTGGCACCTATCATCGAGAAGGCCCTAGAGGGGGAAAAGCTCAGCGTGAAGGAAGT
CGAAGAACTCTTGAGCAGCTGGGACTTGTGGGCCTTAGGGGAGGCAGCGAACGAGCTGAAGAAGCGGATGTACGGGAACG
TGTGTACGTACATAAAGAACATGATACTCAACTACACCAACGAGTGCGTGGTTGAGTGCAAGTTCTGCGCGTTCTACAGG
AGGGTTGGAGAGGGCTACTTGCTCTCCCCAGAAGCTGCCGCCTTCGCGGTTGAGAGGCTGGCGAGGGAGAAGGGCATAAG
GCAAGTCCTAATCCAAGGCGGGGTCAACCCGAAGGTAGATATAGAGTATTTCGAGACCATGTTTAGGGAAATACGCAAGC
GCACCCCTCAAGTAGCCATACACGCCTTGAGCCCCGTGGAGGTGGACTTCCTCAGTAAGAAGCATAAGATCAGTGTGAAA
GAAGTCCTCTCCAGATTGAAGGAAGCGGGCATGGAAAGCATGCCCGGAGGGGGAGCTGAGCTTTTGGTCGAGAGGGTGAG
GAAAATACTCAGCCCTAAGAAGATTTCCGTGGAAAGGTGGCTGGAAATAGTAGAAACTGCCCACAATCTGGGCATACCCA
CGTCCGTAACCATGATGTTTGGCCACGTAGAGACGGTGAGCGAGAGGGCGCTTCACTTGATCAAGCTGAGGGAGCTCCAA
GAGAGGGCCCCGGGGAGCTTGGCCTTCATAGCTTGGAACTTCGAACCCGGGAACACGGAGCTGGCGAGGGAACGTAAAAT
AAAGTACCCCTACGGGGGAGCGGAGCTGTTAAGGGTAATAGCGGTCGCGCGCTTGACCTTCGGGGACCTCATACCTCACG
TCCAAGCGGGCTGGCTGACCGTAGGCAAGGACGTGGCCCAGTTAGCCCTTAAGTGGGGGGCAGACGACTGGGGAGGGACC
TTGTACGAGGAGAGCGTGATACCCGCAACGGGCCTCAAGGTTAAGTTCCCGGACGAGGACGACGTGAGGAGGTGGATAGA
GGGGGCCGGGTGTATAGCCAAGGAGAGGGACAACCTATACCGGGTGGTAGAGTGA

Upstream 100 bases:

>100_bases
GCCGCGCCCAAGAGGCTGGAAGGCTTGGTGGGACGGCTGAGCGAGGAGTTGAAGAAAAGGATTAACCTCCCCCGGGGCGC
GCCTTAAGGGTAAACACTTA

Downstream 100 bases:

>100_bases
TACTCGTAGGCTTGGTCTCGGGAGGTATGGACTCCGCCACCGCCTTCGCTGACGCAATAAACAAGTTCAACCCGGACGTG
AAGGTTGCCCTCACCGTGGT

Product: radical SAM domain-containing protein

Products: NA

Alternate protein names: 7,8-didemethyl-8-hydroxy-5-deazariboflavin synthase subunit 2 [H]

Number of amino acids: Translated: 364; Mature: 364

Protein sequence:

>364_residues
MRTWERSDLAPIIEKALEGEKLSVKEVEELLSSWDLWALGEAANELKKRMYGNVCTYIKNMILNYTNECVVECKFCAFYR
RVGEGYLLSPEAAAFAVERLAREKGIRQVLIQGGVNPKVDIEYFETMFREIRKRTPQVAIHALSPVEVDFLSKKHKISVK
EVLSRLKEAGMESMPGGGAELLVERVRKILSPKKISVERWLEIVETAHNLGIPTSVTMMFGHVETVSERALHLIKLRELQ
ERAPGSLAFIAWNFEPGNTELARERKIKYPYGGAELLRVIAVARLTFGDLIPHVQAGWLTVGKDVAQLALKWGADDWGGT
LYEESVIPATGLKVKFPDEDDVRRWIEGAGCIAKERDNLYRVVE

Sequences:

>Translated_364_residues
MRTWERSDLAPIIEKALEGEKLSVKEVEELLSSWDLWALGEAANELKKRMYGNVCTYIKNMILNYTNECVVECKFCAFYR
RVGEGYLLSPEAAAFAVERLAREKGIRQVLIQGGVNPKVDIEYFETMFREIRKRTPQVAIHALSPVEVDFLSKKHKISVK
EVLSRLKEAGMESMPGGGAELLVERVRKILSPKKISVERWLEIVETAHNLGIPTSVTMMFGHVETVSERALHLIKLRELQ
ERAPGSLAFIAWNFEPGNTELARERKIKYPYGGAELLRVIAVARLTFGDLIPHVQAGWLTVGKDVAQLALKWGADDWGGT
LYEESVIPATGLKVKFPDEDDVRRWIEGAGCIAKERDNLYRVVE
>Mature_364_residues
MRTWERSDLAPIIEKALEGEKLSVKEVEELLSSWDLWALGEAANELKKRMYGNVCTYIKNMILNYTNECVVECKFCAFYR
RVGEGYLLSPEAAAFAVERLAREKGIRQVLIQGGVNPKVDIEYFETMFREIRKRTPQVAIHALSPVEVDFLSKKHKISVK
EVLSRLKEAGMESMPGGGAELLVERVRKILSPKKISVERWLEIVETAHNLGIPTSVTMMFGHVETVSERALHLIKLRELQ
ERAPGSLAFIAWNFEPGNTELARERKIKYPYGGAELLRVIAVARLTFGDLIPHVQAGWLTVGKDVAQLALKWGADDWGGT
LYEESVIPATGLKVKFPDEDDVRRWIEGAGCIAKERDNLYRVVE

Specific function: Catalyzes the radical-mediated transfer of the hydroxybenzyl group from 4-hydroxyphenylpyruvate (HPP) to 5-amino- 6-ribitylamino-2,4(1H,3H)-pyrimidinedione to form 7,8-didemethyl- 8-hydroxy-5-deazariboflavin (FO) [H]

COG id: COG1060

COG function: function code HR; Thiamine biosynthesis enzyme ThiH and related uncharacterized enzymes

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. CofH family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR019940
- InterPro:   IPR006638
- InterPro:   IPR020050
- InterPro:   IPR005244
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 41185; Mature: 41185

Theoretical pI: Translated: 7.10; Mature: 7.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTWERSDLAPIIEKALEGEKLSVKEVEELLSSWDLWALGEAANELKKRMYGNVCTYIKN
CCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
MILNYTNECVVECKFCAFYRRVGEGYLLSPEAAAFAVERLAREKGIRQVLIQGGVNPKVD
HHHHCCHHHHHHHHHHHHHHHHCCCCEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCH
IEYFETMFREIRKRTPQVAIHALSPVEVDFLSKKHKISVKEVLSRLKEAGMESMPGGGAE
HHHHHHHHHHHHHCCCCEEEEECCCCHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCCHH
LLVERVRKILSPKKISVERWLEIVETAHNLGIPTSVTMMFGHVETVSERALHLIKLRELQ
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
ERAPGSLAFIAWNFEPGNTELARERKIKYPYGGAELLRVIAVARLTFGDLIPHVQAGWLT
HHCCCCEEEEEEECCCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHH
VGKDVAQLALKWGADDWGGTLYEESVIPATGLKVKFPDEDDVRRWIEGAGCIAKERDNLY
HHHHHHHHHHHCCCCCCCCCHHHCCCCCCCCCEEECCCHHHHHHHHHHCCCCHHHHCCHH
RVVE
HHCC
>Mature Secondary Structure
MRTWERSDLAPIIEKALEGEKLSVKEVEELLSSWDLWALGEAANELKKRMYGNVCTYIKN
CCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
MILNYTNECVVECKFCAFYRRVGEGYLLSPEAAAFAVERLAREKGIRQVLIQGGVNPKVD
HHHHCCHHHHHHHHHHHHHHHHCCCCEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCH
IEYFETMFREIRKRTPQVAIHALSPVEVDFLSKKHKISVKEVLSRLKEAGMESMPGGGAE
HHHHHHHHHHHHHCCCCEEEEECCCCHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCCHH
LLVERVRKILSPKKISVERWLEIVETAHNLGIPTSVTMMFGHVETVSERALHLIKLRELQ
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
ERAPGSLAFIAWNFEPGNTELARERKIKYPYGGAELLRVIAVARLTFGDLIPHVQAGWLT
HHCCCCEEEEEEECCCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHH
VGKDVAQLALKWGADDWGGTLYEESVIPATGLKVKFPDEDDVRRWIEGAGCIAKERDNLY
HHHHHHHHHHHCCCCCCCCCHHHCCCCCCCCCEEECCCHHHHHHHHHHCCCCHHHHCCHH
RVVE
HHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA