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The map label for this gene is nth [H]

Identifier: 156937657

GI number: 156937657

Start: 765361

End: 765999

Strand: Reverse

Name: nth [H]

Synonym: Igni_0864

Alternate gene names: 156937657

Gene position: 765999-765361 (Counterclockwise)

Preceding gene: 156937658

Following gene: 156937650

Centisome position: 59.03

GC content: 58.53

Gene sequence:

>639_bases
TTGAGGAGGCTGAGGGAAGTCTTCGGCGACTTGGACGACTACGAGAACGAGTTCATCGCGTACTACGTGTACAAGAGGTA
CAAAGACCCCTTCGCCGTGCTCGTGGCGACCGTACTGTCCCAGAACACCACAGAGAAGAACGCCTTCGCGGCTTGGAGGA
ACCTAGAGGAGGCGTTGGGGAGGGTCACTCCCGAGGCGGTCCTCTCGCTCGGAACGGAGAGGTTGAAGGAGCTCATAAGG
CCCGCAGGGCTCCAAGAGCAGAAGGCCTCCGCCATCGTGGAGGCTGCCCGCAAGTGGGAAGAGGTAAAGAAGGCCATAGA
AAAGGGAGACAAAGGCGTCCTAACTAGGATAAAGGGCATAGGCGAGAAAACGGCCGATGTCGTGTTGATGAGCTTCGGAC
ACGAGGAGTTCCCCGTAGACACGCACGTGAAGAGGGTCGCTAAGAGGCTGGGGCTGGTCGACGGAAACGCTTACAAGGAG
GTCTCGTCCCGGCTCAAGGAGCTCTTCAAGGGTAGGACGAGGGAGGCGCACATGTACCTAATACTGCTCGGGAGGAAGTA
CTGTAAGGCTAAAAAGCCTCTGTGCTCGGAGTGCCCGCTCTCCGACCTCTGCCCTAAGCGGGGGGTTTCGGCACGATGA

Upstream 100 bases:

>100_bases
TCGAACACCTGTCCGACATGTTCGCGGCCCTAGAGAAGATGAGGAGGGAAGAGAACTTGCTGGGCATCCTACAGAAGTTC
GTGAGCTCGCTGAGGAGACG

Downstream 100 bases:

>100_bases
CCGCCTTAATCGGGCCCTCCGGGCTAGGCATGTAGAGGACTTTCGACTTGTATATTTCGGTCCTTCTTATCGGGTAGATT
TTCCTAGCTCTGTTTGAGAT

Product: HhH-GPD family protein

Products: NA

Alternate protein names: DNA-(apurinic or apyrimidinic site) lyase [H]

Number of amino acids: Translated: 212; Mature: 212

Protein sequence:

>212_residues
MRRLREVFGDLDDYENEFIAYYVYKRYKDPFAVLVATVLSQNTTEKNAFAAWRNLEEALGRVTPEAVLSLGTERLKELIR
PAGLQEQKASAIVEAARKWEEVKKAIEKGDKGVLTRIKGIGEKTADVVLMSFGHEEFPVDTHVKRVAKRLGLVDGNAYKE
VSSRLKELFKGRTREAHMYLILLGRKYCKAKKPLCSECPLSDLCPKRGVSAR

Sequences:

>Translated_212_residues
MRRLREVFGDLDDYENEFIAYYVYKRYKDPFAVLVATVLSQNTTEKNAFAAWRNLEEALGRVTPEAVLSLGTERLKELIR
PAGLQEQKASAIVEAARKWEEVKKAIEKGDKGVLTRIKGIGEKTADVVLMSFGHEEFPVDTHVKRVAKRLGLVDGNAYKE
VSSRLKELFKGRTREAHMYLILLGRKYCKAKKPLCSECPLSDLCPKRGVSAR
>Mature_212_residues
MRRLREVFGDLDDYENEFIAYYVYKRYKDPFAVLVATVLSQNTTEKNAFAAWRNLEEALGRVTPEAVLSLGTERLKELIR
PAGLQEQKASAIVEAARKWEEVKKAIEKGDKGVLTRIKGIGEKTADVVLMSFGHEEFPVDTHVKRVAKRLGLVDGNAYKE
VSSRLKELFKGRTREAHMYLILLGRKYCKAKKPLCSECPLSDLCPKRGVSAR

Specific function: Has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. Incises damaged DNA at cytosines, thymines and guanines. Acts on a damaged strand, 5' from the damaged site [H]

COG id: COG0177

COG function: function code L; Predicted EndoIII-related endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Escherichia coli, GI1787920, Length=181, Percent_Identity=34.2541436464088, Blast_Score=100, Evalue=8e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR005759
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR023170 [H]

Pfam domain/function: PF10576 EndIII_4Fe-2S; PF00633 HHH; PF00730 HhH-GPD [H]

EC number: =4.2.99.18 [H]

Molecular weight: Translated: 24050; Mature: 24050

Theoretical pI: Translated: 9.86; Mature: 9.86

Prosite motif: PS00764 ENDONUCLEASE_III_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRLREVFGDLDDYENEFIAYYVYKRYKDPFAVLVATVLSQNTTEKNAFAAWRNLEEALG
CCHHHHHHCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
RVTPEAVLSLGTERLKELIRPAGLQEQKASAIVEAARKWEEVKKAIEKGDKGVLTRIKGI
CCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHC
GEKTADVVLMSFGHEEFPVDTHVKRVAKRLGLVDGNAYKEVSSRLKELFKGRTREAHMYL
CHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCHHHHHH
ILLGRKYCKAKKPLCSECPLSDLCPKRGVSAR
HHHHHHHHHHCCCCCCCCCCHHCCCCCCCCCC
>Mature Secondary Structure
MRRLREVFGDLDDYENEFIAYYVYKRYKDPFAVLVATVLSQNTTEKNAFAAWRNLEEALG
CCHHHHHHCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
RVTPEAVLSLGTERLKELIRPAGLQEQKASAIVEAARKWEEVKKAIEKGDKGVLTRIKGI
CCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHC
GEKTADVVLMSFGHEEFPVDTHVKRVAKRLGLVDGNAYKEVSSRLKELFKGRTREAHMYL
CHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCHHHHHH
ILLGRKYCKAKKPLCSECPLSDLCPKRGVSAR
HHHHHHHHHHCCCCCCCCCCHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA