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The map label for this gene is hisF

Identifier: 156937566

GI number: 156937566

Start: 689798

End: 690565

Strand: Direct

Name: hisF

Synonym: Igni_0773

Alternate gene names: 156937566

Gene position: 689798-690565 (Clockwise)

Preceding gene: 156937565

Following gene: 156937568

Centisome position: 53.16

GC content: 56.9

Gene sequence:

>768_bases
TTGCCGCTAACTAAGAGAATAATCCCTTGTTTGGACGTAAAGGACGGTAGGGTCGTGAAGGGCGTGAGCTTCCAGAACTT
GAGGGACGCCGGGGACCCGGTAGAGCTGGCCGCGTACTATCAAGAACAAGGCGCCGACGAGATAGTCTTCTTGGACATAT
CCGCCACCCCCGAAGGGAGGGAAACGATGATTGAGGTAGTAAGGAGGACTGCGGAAAACCTCAGCATTCCCCTGACCGTC
GGCGGAGGGGTCCGGAGCTTGGAGCATATAGAAAAACTGCTCAAGGCCGGCGCGGACAAAGTAAGCATAAACACCGCCGC
CGTGAAGAACCCCTTGCTAATAACTGCGGCCGCGGAGGAGTTCGGCTCCCAAGCAGTGGTCGTCGCGATAGACGCTAAAA
GAAAGGGAAACGGGTGGGAGGTTTACGTCAGCGCGGGCAAGGTGCCTACGGGGCTGGACGCAGTCGAGTGGGCGAAGAAG
GCGGAGGAGTTAGGGGCCGGTGAGATACTCCTTACCTCAATAGACTACGACGGGACCCAGAACGGGTACGACTTAGAACT
GACGAGGGCGGTAAGCGAGGCAGTCAAGATACCGGTAATAGCTTCTGGAGGCGCCGGCGAGCTCGAACACTTCTACGCAG
TTCTAACTGAAGGGAAGGCTGACGCGGCACTGGCGGCGAGCGTTTTCCACTACGGTAAGTTCACCGTCGGAGATGTCAAA
AAATACCTAATTCAGCGCGGAGTGCCGGTAAGGCCTTGTTGTTGGTAG

Upstream 100 bases:

>100_bases
TGGTGATACTGGCTGTATTGGCGTTAATATTTAGGAAGGTGGGCGAGTAAACTCGTTTTTAGCGTTCTACCTCCCGTCCC
CGTCCTCGGAGGATCTCGCT

Downstream 100 bases:

>100_bases
GCTGCGTAGAGCTCCTTCATAGTCTTAGTTACTAGGAAGTACTCAAACGCGTACGACGCGCTGATAGGGAGGGACATCAG
CATTTTGTGAACGAAGCCAA

Product: imidazole glycerol phosphate synthase subunit hisF

Products: NA

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF; ImGP synthase subunit hisF; IGPS subunit hisF

Number of amino acids: Translated: 255; Mature: 254

Protein sequence:

>255_residues
MPLTKRIIPCLDVKDGRVVKGVSFQNLRDAGDPVELAAYYQEQGADEIVFLDISATPEGRETMIEVVRRTAENLSIPLTV
GGGVRSLEHIEKLLKAGADKVSINTAAVKNPLLITAAAEEFGSQAVVVAIDAKRKGNGWEVYVSAGKVPTGLDAVEWAKK
AEELGAGEILLTSIDYDGTQNGYDLELTRAVSEAVKIPVIASGGAGELEHFYAVLTEGKADAALAASVFHYGKFTVGDVK
KYLIQRGVPVRPCCW

Sequences:

>Translated_255_residues
MPLTKRIIPCLDVKDGRVVKGVSFQNLRDAGDPVELAAYYQEQGADEIVFLDISATPEGRETMIEVVRRTAENLSIPLTV
GGGVRSLEHIEKLLKAGADKVSINTAAVKNPLLITAAAEEFGSQAVVVAIDAKRKGNGWEVYVSAGKVPTGLDAVEWAKK
AEELGAGEILLTSIDYDGTQNGYDLELTRAVSEAVKIPVIASGGAGELEHFYAVLTEGKADAALAASVFHYGKFTVGDVK
KYLIQRGVPVRPCCW
>Mature_254_residues
PLTKRIIPCLDVKDGRVVKGVSFQNLRDAGDPVELAAYYQEQGADEIVFLDISATPEGRETMIEVVRRTAENLSIPLTVG
GGVRSLEHIEKLLKAGADKVSINTAAVKNPLLITAAAEEFGSQAVVVAIDAKRKGNGWEVYVSAGKVPTGLDAVEWAKKA
EELGAGEILLTSIDYDGTQNGYDLELTRAVSEAVKIPVIASGGAGELEHFYAVLTEGKADAALAASVFHYGKFTVGDVKK
YLIQRGVPVRPCCW

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family

Homologues:

Organism=Escherichia coli, GI1788336, Length=258, Percent_Identity=46.8992248062016, Blast_Score=226, Evalue=1e-60,
Organism=Escherichia coli, GI87082028, Length=238, Percent_Identity=30.672268907563, Blast_Score=93, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6319725, Length=315, Percent_Identity=38.4126984126984, Blast_Score=181, Evalue=7e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS6_IGNH4 (A8AAK3)

Other databases:

- EMBL:   CP000816
- RefSeq:   YP_001435362.1
- ProteinModelPortal:   A8AAK3
- SMR:   A8AAK3
- STRING:   A8AAK3
- GeneID:   5562110
- GenomeReviews:   CP000816_GR
- KEGG:   iho:Igni_0773
- eggNOG:   arNOG04606
- HOGENOM:   HBG541613
- OMA:   RVVKGTN
- BioCyc:   IHOS453591:IGNI_0773-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01013
- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00735

Pfam domain/function: PF00977 His_biosynth; SSF51366 RibP_bind_barrel

EC number: 4.1.3.-

Molecular weight: Translated: 27257; Mature: 27126

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: NA

Important sites: ACT_SITE 12-12 ACT_SITE 131-131

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLTKRIIPCLDVKDGRVVKGVSFQNLRDAGDPVELAAYYQEQGADEIVFLDISATPEGR
CCCCHHCCCEECCCCCCEEECCCHHHHHCCCCHHHHHHHHHHCCCCEEEEEEEECCCCHH
ETMIEVVRRTAENLSIPLTVGGGVRSLEHIEKLLKAGADKVSINTAAVKNPLLITAAAEE
HHHHHHHHHHHHCCEEEEEECCCHHHHHHHHHHHHCCCCEEEEEHHHHCCCEEEEEEHHH
FGSQAVVVAIDAKRKGNGWEVYVSAGKVPTGLDAVEWAKKAEELGAGEILLTSIDYDGTQ
HCCCEEEEEEECCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCCEEEEEEECCCCCC
NGYDLELTRAVSEAVKIPVIASGGAGELEHFYAVLTEGKADAALAASVFHYGKFTVGDVK
CCCCCHHHHHHHHHHCCEEEECCCCCHHHHHHHHHCCCCCHHHHHHHHHHCCCCCHHHHH
KYLIQRGVPVRPCCW
HHHHHCCCCCCCCCC
>Mature Secondary Structure 
PLTKRIIPCLDVKDGRVVKGVSFQNLRDAGDPVELAAYYQEQGADEIVFLDISATPEGR
CCCHHCCCEECCCCCCEEECCCHHHHHCCCCHHHHHHHHHHCCCCEEEEEEEECCCCHH
ETMIEVVRRTAENLSIPLTVGGGVRSLEHIEKLLKAGADKVSINTAAVKNPLLITAAAEE
HHHHHHHHHHHHCCEEEEEECCCHHHHHHHHHHHHCCCCEEEEEHHHHCCCEEEEEEHHH
FGSQAVVVAIDAKRKGNGWEVYVSAGKVPTGLDAVEWAKKAEELGAGEILLTSIDYDGTQ
HCCCEEEEEEECCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCCEEEEEEECCCCCC
NGYDLELTRAVSEAVKIPVIASGGAGELEHFYAVLTEGKADAALAASVFHYGKFTVGDVK
CCCCCHHHHHHHHHHCCEEEECCCCCHHHHHHHHHCCCCCHHHHHHHHHHCCCCCHHHHH
KYLIQRGVPVRPCCW
HHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA