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The map label for this gene is moaA [H]

Identifier: 156937491

GI number: 156937491

Start: 630530

End: 631588

Strand: Reverse

Name: moaA [H]

Synonym: Igni_0698

Alternate gene names: 156937491

Gene position: 631588-630530 (Counterclockwise)

Preceding gene: 156937492

Following gene: 156937490

Centisome position: 48.68

GC content: 51.27

Gene sequence:

>1059_bases
ATGATCCGCGACGCCTACGGCAGAAGCCTGGCGACCTTAAGGGTGTCCGTTACGGAGAGGTGTAACTTCAATTGCATATT
CTGCCACTCCGAAGGCGCGGGTAGAGGCTCGTTTGACGAGCTCTCCGTCAACGATTACGACATGATAGCGGAAGCCACCT
CGAGGCTCGGATTAAAGTACGTCAAGTTCACCGGGGGCGAGCCGTTGCTCCGAAGCGACCTAGAAGAAATAATTCATTCA
TTTAAAGAACACGGGTTCGAGGAGATATCCATCACTACCAACGGCTTCTTGCTGCCGGAGAGGACAGAGGGCCTCAAGGA
GGCCGGCGTGAGTTGGATAAACGTTAGTTTACACAGCCTCAAGAGGCAGCGATTTAGAAGGATAACGGGCGTCGACGCCC
TTAACCGCGTGTTGAACGGGATAGAAAAGGCTTTAGAAAACGGCATAGAGGTCAGGGTAAATGTGGTCGTTTTGAGGGGG
ATAAACGAGGACGAGGTGGAAGAGATAGTGAAGTACGCCATAGGAAAGGGAGCCTCCGTTCACGTGATCGAGTTGCACCC
AGTAGGCAACGGGGCCCACATATTCCGCGAGCGCCACTCAAGAGAGCCCATAGAGCGGTTGAAGAAGTGGCTCGAGGAGG
TTGCTGATAAAAAGGAAGTCAGAGAACTACACAATAGGCCTAGGTATTACTTGGGCAAGAACTTCGTCGAGCTGATAGAG
CCCGTCGGCAACCCTTACTTCTGCGCGGGGTGCACGCGACTGAGGTTGAGCGCGGACGGTAAGTTCTACCCTTGTATAAA
CGTTTACCAAACGCATTTTGACGCTTCCAGAGTGTTACGATCCGGCGAGTCTAGGGAAATTAAAATAATGAAAATAATAG
AGGGCATTCTAGAACTCAACGATAAGAGGAGACCATACTACATGTGGAACTTAGACTTCGAGGCTAAAGGTTTGATGGTT
AAAAAGGGGATCCAAGTAGGCAGGATCGGGCTGCCGAAGAGGGCTAAACTTCAATTCCGCTCGGCGAGGGTAAGAGAGGG
AAGACCTCTTGAAGCGTAA

Upstream 100 bases:

>100_bases
GTTAAATCAGTACTGTACCTCTTGGTCGCTGCCGCCTTCGAGGTAACGGCAATACGTTTCTAACGCTCTAGTCGCTTTAG
AGATCCAGAGATGTCGGGCT

Downstream 100 bases:

>100_bases
TTACGTGGTCGCCCGGTACGAGTACAAGGGCCACAAGTTCGAAATCTTGGTGGACCCCGATAAAGCTCTAGAACTCAAGT
CCGGTAAGCAAATACCGATA

Product: radical SAM domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 352; Mature: 352

Protein sequence:

>352_residues
MIRDAYGRSLATLRVSVTERCNFNCIFCHSEGAGRGSFDELSVNDYDMIAEATSRLGLKYVKFTGGEPLLRSDLEEIIHS
FKEHGFEEISITTNGFLLPERTEGLKEAGVSWINVSLHSLKRQRFRRITGVDALNRVLNGIEKALENGIEVRVNVVVLRG
INEDEVEEIVKYAIGKGASVHVIELHPVGNGAHIFRERHSREPIERLKKWLEEVADKKEVRELHNRPRYYLGKNFVELIE
PVGNPYFCAGCTRLRLSADGKFYPCINVYQTHFDASRVLRSGESREIKIMKIIEGILELNDKRRPYYMWNLDFEAKGLMV
KKGIQVGRIGLPKRAKLQFRSARVREGRPLEA

Sequences:

>Translated_352_residues
MIRDAYGRSLATLRVSVTERCNFNCIFCHSEGAGRGSFDELSVNDYDMIAEATSRLGLKYVKFTGGEPLLRSDLEEIIHS
FKEHGFEEISITTNGFLLPERTEGLKEAGVSWINVSLHSLKRQRFRRITGVDALNRVLNGIEKALENGIEVRVNVVVLRG
INEDEVEEIVKYAIGKGASVHVIELHPVGNGAHIFRERHSREPIERLKKWLEEVADKKEVRELHNRPRYYLGKNFVELIE
PVGNPYFCAGCTRLRLSADGKFYPCINVYQTHFDASRVLRSGESREIKIMKIIEGILELNDKRRPYYMWNLDFEAKGLMV
KKGIQVGRIGLPKRAKLQFRSARVREGRPLEA
>Mature_352_residues
MIRDAYGRSLATLRVSVTERCNFNCIFCHSEGAGRGSFDELSVNDYDMIAEATSRLGLKYVKFTGGEPLLRSDLEEIIHS
FKEHGFEEISITTNGFLLPERTEGLKEAGVSWINVSLHSLKRQRFRRITGVDALNRVLNGIEKALENGIEVRVNVVVLRG
INEDEVEEIVKYAIGKGASVHVIELHPVGNGAHIFRERHSREPIERLKKWLEEVADKKEVRELHNRPRYYLGKNFVELIE
PVGNPYFCAGCTRLRLSADGKFYPCINVYQTHFDASRVLRSGESREIKIMKIIEGILELNDKRRPYYMWNLDFEAKGLMV
KKGIQVGRIGLPKRAKLQFRSARVREGRPLEA

Specific function: Together with moaC, is involved in the conversion of a guanosine derivative (5'-GTP) into molybdopterin precursor Z [H]

COG id: COG2896

COG function: function code H; Molybdenum cofactor biosynthesis enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. MoaA family [H]

Homologues:

Organism=Homo sapiens, GI116734668, Length=306, Percent_Identity=31.0457516339869, Blast_Score=129, Evalue=4e-30,
Organism=Homo sapiens, GI22027536, Length=307, Percent_Identity=30.9446254071661, Blast_Score=129, Evalue=4e-30,
Organism=Escherichia coli, GI1786999, Length=275, Percent_Identity=33.0909090909091, Blast_Score=155, Evalue=3e-39,
Organism=Caenorhabditis elegans, GI17561210, Length=276, Percent_Identity=31.5217391304348, Blast_Score=132, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI32566329, Length=274, Percent_Identity=31.3868613138686, Blast_Score=122, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI32566331, Length=274, Percent_Identity=31.3868613138686, Blast_Score=121, Evalue=6e-28,
Organism=Drosophila melanogaster, GI28574488, Length=277, Percent_Identity=32.8519855595668, Blast_Score=130, Evalue=1e-30,
Organism=Drosophila melanogaster, GI28574490, Length=277, Percent_Identity=32.8519855595668, Blast_Score=130, Evalue=2e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR013485
- InterPro:   IPR000385
- InterPro:   IPR010505
- InterPro:   IPR007197 [H]

Pfam domain/function: PF06463 Mob_synth_C; PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 40272; Mature: 40272

Theoretical pI: Translated: 9.27; Mature: 9.27

Prosite motif: PS01305 MOAA_NIFB_PQQE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRDAYGRSLATLRVSVTERCNFNCIFCHSEGAGRGSFDELSVNDYDMIAEATSRLGLKY
CCCCCCCCCEEEEEEEECCCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHCCEEE
VKFTGGEPLLRSDLEEIIHSFKEHGFEEISITTNGFLLPERTEGLKEAGVSWINVSLHSL
EEECCCCHHHHHHHHHHHHHHHHCCCEEEEEEECCEECCHHHCCHHHCCCEEEEHHHHHH
KRQRFRRITGVDALNRVLNGIEKALENGIEVRVNVVVLRGINEDEVEEIVKYAIGKGASV
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEECCCHHHHHHHHHHHHCCCCEE
HVIELHPVGNGAHIFRERHSREPIERLKKWLEEVADKKEVRELHNRPRYYLGKNFVELIE
EEEEEEECCCCHHHHHHHHCCCHHHHHHHHHHHHHCHHHHHHHHCCCCCHHHHHHHHHHH
PVGNPYFCAGCTRLRLSADGKFYPCINVYQTHFDASRVLRSGESREIKIMKIIEGILELN
HCCCCEEEECCEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHCC
DKRRPYYMWNLDFEAKGLMVKKGIQVGRIGLPKRAKLQFRSARVREGRPLEA
CCCCCEEEEEECCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MIRDAYGRSLATLRVSVTERCNFNCIFCHSEGAGRGSFDELSVNDYDMIAEATSRLGLKY
CCCCCCCCCEEEEEEEECCCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHCCEEE
VKFTGGEPLLRSDLEEIIHSFKEHGFEEISITTNGFLLPERTEGLKEAGVSWINVSLHSL
EEECCCCHHHHHHHHHHHHHHHHCCCEEEEEEECCEECCHHHCCHHHCCCEEEEHHHHHH
KRQRFRRITGVDALNRVLNGIEKALENGIEVRVNVVVLRGINEDEVEEIVKYAIGKGASV
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEECCCHHHHHHHHHHHHCCCCEE
HVIELHPVGNGAHIFRERHSREPIERLKKWLEEVADKKEVRELHNRPRYYLGKNFVELIE
EEEEEEECCCCHHHHHHHHCCCHHHHHHHHHHHHHCHHHHHHHHCCCCCHHHHHHHHHHH
PVGNPYFCAGCTRLRLSADGKFYPCINVYQTHFDASRVLRSGESREIKIMKIIEGILELN
HCCCCEEEECCEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHCC
DKRRPYYMWNLDFEAKGLMVKKGIQVGRIGLPKRAKLQFRSARVREGRPLEA
CCCCCEEEEEECCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10382966 [H]