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The map label for this gene is fen

Identifier: 156937484

GI number: 156937484

Start: 624002

End: 625054

Strand: Reverse

Name: fen

Synonym: Igni_0691

Alternate gene names: 156937484

Gene position: 625054-624002 (Counterclockwise)

Preceding gene: 156937485

Following gene: 156937483

Centisome position: 48.17

GC content: 54.8

Gene sequence:

>1053_bases
TTGGGCGTTACAGCCCTAAGGGAACTCATCCCCAGCAAGTGCAAGAAGACCTTAGAGCTAAAGTCCTTGTCGAACAAGAG
CGTGGCCCTCGACGCTTACAACACCTTGTATCAGTTCTTAGCTGCCATAAGGGGCGAAGACGGCAGGCCCCTCATGGACT
CCAAAGGGCGCGTGACCAGCCACCTCTCCGGACTTTTCTACAGAACAATCAACATGTTGGAAAACGGAATAAAGGTAGCC
TACGTCTTCGACGGCGCCCCTCCCAAGCTCAAGACGCGCGAGATAGAGAGGAGGCAGAAACTCAAGCAAGAGGCCGAGAA
GAAGTACGAGGAGGCAGTTAGGAGGGGGGACGTCGAGGAAGCTAGGAAGTACGCCCAGATGAGCGCAAAGCTGACGAAGG
AGATGGTAGAAGAAGCTAAGAGGTTGCTCGAGGCTATGGGGGTCCCGTGGGTACAAGCCCCCAGCGAGGGAGAGGCCCAA
GCGGCCTACATGGCCGCTAAGGGAGACGTCTGGGCGTCTGCTAGTCAAGATTACGACTCCTTGCTCTTCGGCTCTCCTCG
CTTGGTTAGGAACTTGGCCGTGAGTGGGCGCAGGAAGCTCCCTAACAAGAACGTGTACGTCGAAGTGAAGCCCGAGGAAA
TAACTTTGAAGTGTGTGCTGGAGGAGTTGGGCATAACCCGAGAGCAGCTCGTTGCAATAGCCGTCTTGATAGGGACCGAC
TACACGCCCGGGGTGAAGGGCGTCGGGCCGAAGACCGCCTTAAGGTACGTGAAGAGCTATGGTGACTTAGAGAGGGTGCT
TACTGCCCTAGGCGTCGATGACAAGGAGTTGTACTTGGAGGCGTATAATTTCTTCTTGAACCCCCAGGTGACCGACGACT
ACGAGCTCGTGTGGAGGAGGCCCGACCCCCAAAAGATAATTGAAATCCTAGTGTACGAACACGACTTCAACGAGGAGCGC
GTGAGAAAGGCGATAGAGCGCTTAATGAAGGCCTGGAAGGAAAAGCTCAGCACTAAGCAGAGCACGTTGGACATGTTCTT
TAAAAAGCGTTGA

Upstream 100 bases:

>100_bases
AGACTATGAGTAATGGAGAAGACGTGAGCCGACTGAGCGTAGCCCTTATCCAGAACGAGCGATAAACCCTCTCGTCGTCG
AAATCGCTTAGGGAAGTGTC

Downstream 100 bases:

>100_bases
GGCTTAGAGCCCTCGGGAGTACCGCGGGCTTATCAACCCATCTCCCCCCTAAGGCTAGGCCGGGGCTAAAGTAGTGAAGA
AACCCCGAAGGGGACCGACG

Product: flap endonuclease-1

Products: NA

Alternate protein names: FEN-1; Flap structure-specific endonuclease 1

Number of amino acids: Translated: 350; Mature: 349

Protein sequence:

>350_residues
MGVTALRELIPSKCKKTLELKSLSNKSVALDAYNTLYQFLAAIRGEDGRPLMDSKGRVTSHLSGLFYRTINMLENGIKVA
YVFDGAPPKLKTREIERRQKLKQEAEKKYEEAVRRGDVEEARKYAQMSAKLTKEMVEEAKRLLEAMGVPWVQAPSEGEAQ
AAYMAAKGDVWASASQDYDSLLFGSPRLVRNLAVSGRRKLPNKNVYVEVKPEEITLKCVLEELGITREQLVAIAVLIGTD
YTPGVKGVGPKTALRYVKSYGDLERVLTALGVDDKELYLEAYNFFLNPQVTDDYELVWRRPDPQKIIEILVYEHDFNEER
VRKAIERLMKAWKEKLSTKQSTLDMFFKKR

Sequences:

>Translated_350_residues
MGVTALRELIPSKCKKTLELKSLSNKSVALDAYNTLYQFLAAIRGEDGRPLMDSKGRVTSHLSGLFYRTINMLENGIKVA
YVFDGAPPKLKTREIERRQKLKQEAEKKYEEAVRRGDVEEARKYAQMSAKLTKEMVEEAKRLLEAMGVPWVQAPSEGEAQ
AAYMAAKGDVWASASQDYDSLLFGSPRLVRNLAVSGRRKLPNKNVYVEVKPEEITLKCVLEELGITREQLVAIAVLIGTD
YTPGVKGVGPKTALRYVKSYGDLERVLTALGVDDKELYLEAYNFFLNPQVTDDYELVWRRPDPQKIIEILVYEHDFNEER
VRKAIERLMKAWKEKLSTKQSTLDMFFKKR
>Mature_349_residues
GVTALRELIPSKCKKTLELKSLSNKSVALDAYNTLYQFLAAIRGEDGRPLMDSKGRVTSHLSGLFYRTINMLENGIKVAY
VFDGAPPKLKTREIERRQKLKQEAEKKYEEAVRRGDVEEARKYAQMSAKLTKEMVEEAKRLLEAMGVPWVQAPSEGEAQA
AYMAAKGDVWASASQDYDSLLFGSPRLVRNLAVSGRRKLPNKNVYVEVKPEEITLKCVLEELGITREQLVAIAVLIGTDY
TPGVKGVGPKTALRYVKSYGDLERVLTALGVDDKELYLEAYNFFLNPQVTDDYELVWRRPDPQKIIEILVYEHDFNEERV
RKAIERLMKAWKEKLSTKQSTLDMFFKKR

Specific function: Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymeras

COG id: COG0258

COG function: function code L; 5'-3' exonuclease (including N-terminal domain of PolI)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the XPG/RAD2 endonuclease family. FEN1 subfamily

Homologues:

Organism=Homo sapiens, GI4758356, Length=359, Percent_Identity=39.2757660167131, Blast_Score=245, Evalue=4e-65,
Organism=Homo sapiens, GI194018535, Length=259, Percent_Identity=29.3436293436293, Blast_Score=116, Evalue=3e-26,
Organism=Homo sapiens, GI194018531, Length=259, Percent_Identity=29.3436293436293, Blast_Score=116, Evalue=3e-26,
Organism=Homo sapiens, GI284055276, Length=335, Percent_Identity=25.9701492537313, Blast_Score=83, Evalue=5e-16,
Organism=Homo sapiens, GI284172361, Length=335, Percent_Identity=25.9701492537313, Blast_Score=82, Evalue=6e-16,
Organism=Homo sapiens, GI284055278, Length=335, Percent_Identity=25.9701492537313, Blast_Score=82, Evalue=6e-16,
Organism=Homo sapiens, GI51988900, Length=256, Percent_Identity=26.5625, Blast_Score=76, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI17510005, Length=360, Percent_Identity=38.8888888888889, Blast_Score=253, Evalue=2e-67,
Organism=Saccharomyces cerevisiae, GI6322736, Length=364, Percent_Identity=39.5604395604396, Blast_Score=248, Evalue=1e-66,
Organism=Saccharomyces cerevisiae, GI6321697, Length=195, Percent_Identity=30.7692307692308, Blast_Score=86, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6320469, Length=283, Percent_Identity=26.1484098939929, Blast_Score=83, Evalue=7e-17,
Organism=Saccharomyces cerevisiae, GI6324607, Length=229, Percent_Identity=26.6375545851528, Blast_Score=80, Evalue=5e-16,
Organism=Drosophila melanogaster, GI17647423, Length=358, Percent_Identity=39.9441340782123, Blast_Score=248, Evalue=6e-66,
Organism=Drosophila melanogaster, GI24658219, Length=249, Percent_Identity=24.0963855421687, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI17137168, Length=263, Percent_Identity=25.4752851711027, Blast_Score=76, Evalue=3e-14,
Organism=Drosophila melanogaster, GI78706850, Length=144, Percent_Identity=30.5555555555556, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI78706852, Length=144, Percent_Identity=30.5555555555556, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI78706854, Length=144, Percent_Identity=30.5555555555556, Blast_Score=74, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FEN_IGNH4 (A8AAC1)

Other databases:

- EMBL:   CP000816
- RefSeq:   YP_001435280.1
- ProteinModelPortal:   A8AAC1
- SMR:   A8AAC1
- STRING:   A8AAC1
- GeneID:   5562892
- GenomeReviews:   CP000816_GR
- KEGG:   iho:Igni_0691
- eggNOG:   arNOG04505
- HOGENOM:   HBG316651
- OMA:   DYDSLLF
- ProtClustDB:   PRK03980
- BioCyc:   IHOS453591:IGNI_0691-MONOMER
- HAMAP:   MF_00614
- InterPro:   IPR020045
- InterPro:   IPR019973
- InterPro:   IPR008918
- InterPro:   IPR006086
- InterPro:   IPR019974
- InterPro:   IPR006085
- InterPro:   IPR006084
- PANTHER:   PTHR11081
- PRINTS:   PR00853
- SMART:   SM00279
- SMART:   SM00484
- SMART:   SM00485
- TIGRFAMs:   TIGR03674

Pfam domain/function: PF00867 XPG_I; PF00752 XPG_N; SSF47807 5_3_exo_C

EC number: 3.-.-.-

Molecular weight: Translated: 39911; Mature: 39779

Theoretical pI: Translated: 9.54; Mature: 9.54

Prosite motif: PS00841 XPG_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGVTALRELIPSKCKKTLELKSLSNKSVALDAYNTLYQFLAAIRGEDGRPLMDSKGRVTS
CCHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHH
HLSGLFYRTINMLENGIKVAYVFDGAPPKLKTREIERRQKLKQEAEKKYEEAVRRGDVEE
HHHHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
ARKYAQMSAKLTKEMVEEAKRLLEAMGVPWVQAPSEGEAQAAYMAAKGDVWASASQDYDS
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCHHHHHEEECCCCCCCCCCCHHH
LLFGSPRLVRNLAVSGRRKLPNKNVYVEVKPEEITLKCVLEELGITREQLVAIAVLIGTD
HHCCCHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHCCCHHHHHHHHHHHCCC
YTPGVKGVGPKTALRYVKSYGDLERVLTALGVDDKELYLEAYNFFLNPQVTDDYELVWRR
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCHHEEECC
PDPQKIIEILVYEHDFNEERVRKAIERLMKAWKEKLSTKQSTLDMFFKKR
CCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
GVTALRELIPSKCKKTLELKSLSNKSVALDAYNTLYQFLAAIRGEDGRPLMDSKGRVTS
CHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHH
HLSGLFYRTINMLENGIKVAYVFDGAPPKLKTREIERRQKLKQEAEKKYEEAVRRGDVEE
HHHHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
ARKYAQMSAKLTKEMVEEAKRLLEAMGVPWVQAPSEGEAQAAYMAAKGDVWASASQDYDS
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCHHHHHEEECCCCCCCCCCCHHH
LLFGSPRLVRNLAVSGRRKLPNKNVYVEVKPEEITLKCVLEELGITREQLVAIAVLIGTD
HHCCCHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHCCCHHHHHHHHHHHCCC
YTPGVKGVGPKTALRYVKSYGDLERVLTALGVDDKELYLEAYNFFLNPQVTDDYELVWRR
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCHHEEECC
PDPQKIIEILVYEHDFNEERVRKAIERLMKAWKEKLSTKQSTLDMFFKKR
CCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA