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The map label for this gene is fumA [H]

Identifier: 156937471

GI number: 156937471

Start: 610990

End: 612468

Strand: Direct

Name: fumA [H]

Synonym: Igni_0678

Alternate gene names: 156937471

Gene position: 610990-612468 (Clockwise)

Preceding gene: 156937460

Following gene: 156937472

Centisome position: 47.09

GC content: 58.49

Gene sequence:

>1479_bases
TTGCTTAGGGATGAGGTCGTAAACGCTGTGGTAGAGGCCATAAGGCTCGCCGAAACCGCGCTGCCCAAGTGGGTTTATGA
AAAGCTGCTGGACGCTTACGAGCGCTCAGAGGGGTTGGGGAAGGCCCAGCTGGCGGCCATTTTGAACAACATAGAGTTGG
CCGTCAAGGAGAAGAAGCCCATGTGTCAAGATACGGGCTTGATGACCTTTGATGTTATAGTTGGTGACGAGTTCCCAATA
AAGCCTTACGAAATTGAGGAGCTTCTGAAGGAAGCCGTGAGGAGGGCCACGCGAGAGGTCCCCATAAGGCCCAACAGCGT
GGACCCGCTAAGCGGCCACAACCCCGGCGACAACGTCGGGAAGGGCGTGCCAGTCACAAATATTAAGGTAGTCCCGGGCA
ACACGCTCCTAATAAAAGTAAGGCCTAAGGGAGGGGGGAGCGAGTACCCCTCCAATTTGTGCATGATACCGCCTTCCAAG
GGGTTGGAAGGCGTTAGGGAGTGTGTGCTCAAGGCCGTCTTCGAGGCCGGGGGCAAGCCTTGTCCTCCGGGCATAGTCGG
CGTCGCCTTCGGAGGGACCGTGGAAGAGACCGCTAAGCTCGCAAAAGAGGCCCTCTACAGGCCGGAGGCCCACGAGGAGG
AGAGGGTTGCGAGGATCGAAAAAGAGCTGCTGGACGAAGTGAACTCCTTGGGCTTGGGTCCAATGGGCTTGGGCGGGGGG
CCCACGGCGCTCGCGGTGAGGGCGGACTACGCTTACCGGCACCCGGCCTCGTTCCCGGTTGCGGTTAGGTTCAACTGCTG
GGCCGCGCGCGAGGCCGTAGTTGAGGTTAACGCCGACGGGAGCTGGAAATTGGTAAGCGACAACGTAACTCCGGAGGACT
TGAAGACCCCGCCGTTGGAGGCGGAGGACGGCGTTGAGATAAAGTTACCCGTTGAAGAGGAAGAAGTGAGGAAGTTGAAG
GCCGGAGACGTGGTTTACTTGACGGGCACCGTGGTTACTGCGAGGGACGAGGCTCACAAGAAGATAATTGAGGAGGGGGC
CCCGCTCGACCTAAAAGGTCTAGCCATATACCATTGTGGACCCGTCGTGACGAAGGAGGAGGGCCGGTGGAAGGTGGTGG
CGGCCGGCCCCACTACCAGCGCGAGGATGAACGCCCTCCAAGCTCAAGTGCTGGAAAAGACCGGGGCCAGGCTAGTAATA
GGTAAGGGCGGTATGAAAGAAGACCTCTTGGACTTCTTTAAGAGGTTCGGGGCGGCCTACTTGGCCTTCCCTGGCGGCGC
CGCGCTCTTGGCCGCCAAGGCGATAAAGGCTGTCAGGGGGGTATACTGGTTAGAGGAGCTCGGGATACCAGAAGCCATGT
GGGTTTTCGAGGTAGAGAAGTTCGGGCCCTTAGTGGTGGCCATGGACTCCCACGGAAGGAGCTTATACAAGGAAGTCTCT
GAGAGAGCCAAGACTAAACTAGAGGAGCTTCTGAAGTGA

Upstream 100 bases:

>100_bases
ACTACCTTGAACTCTAGCTTAGCCACCTCAAACCTCACCGGGCGCGCGACGTTTGGCAAGTTATTAACGATCTCTCGCGC
CCCCTCGAGGTGTACACAGC

Downstream 100 bases:

>100_bases
CCGCGCCGCACGCCGAAAATTCAAAATTTAAGCAGTCCTTCTCGTCTCTTCAAGGGAGGTCTCACTTTGGCAGTACCGTC
GGTTAAGGGCAAGGAGTACG

Product: fumarase

Products: NA

Alternate protein names: Fumarase [H]

Number of amino acids: Translated: 492; Mature: 492

Protein sequence:

>492_residues
MLRDEVVNAVVEAIRLAETALPKWVYEKLLDAYERSEGLGKAQLAAILNNIELAVKEKKPMCQDTGLMTFDVIVGDEFPI
KPYEIEELLKEAVRRATREVPIRPNSVDPLSGHNPGDNVGKGVPVTNIKVVPGNTLLIKVRPKGGGSEYPSNLCMIPPSK
GLEGVRECVLKAVFEAGGKPCPPGIVGVAFGGTVEETAKLAKEALYRPEAHEEERVARIEKELLDEVNSLGLGPMGLGGG
PTALAVRADYAYRHPASFPVAVRFNCWAAREAVVEVNADGSWKLVSDNVTPEDLKTPPLEAEDGVEIKLPVEEEEVRKLK
AGDVVYLTGTVVTARDEAHKKIIEEGAPLDLKGLAIYHCGPVVTKEEGRWKVVAAGPTTSARMNALQAQVLEKTGARLVI
GKGGMKEDLLDFFKRFGAAYLAFPGGAALLAAKAIKAVRGVYWLEELGIPEAMWVFEVEKFGPLVVAMDSHGRSLYKEVS
ERAKTKLEELLK

Sequences:

>Translated_492_residues
MLRDEVVNAVVEAIRLAETALPKWVYEKLLDAYERSEGLGKAQLAAILNNIELAVKEKKPMCQDTGLMTFDVIVGDEFPI
KPYEIEELLKEAVRRATREVPIRPNSVDPLSGHNPGDNVGKGVPVTNIKVVPGNTLLIKVRPKGGGSEYPSNLCMIPPSK
GLEGVRECVLKAVFEAGGKPCPPGIVGVAFGGTVEETAKLAKEALYRPEAHEEERVARIEKELLDEVNSLGLGPMGLGGG
PTALAVRADYAYRHPASFPVAVRFNCWAAREAVVEVNADGSWKLVSDNVTPEDLKTPPLEAEDGVEIKLPVEEEEVRKLK
AGDVVYLTGTVVTARDEAHKKIIEEGAPLDLKGLAIYHCGPVVTKEEGRWKVVAAGPTTSARMNALQAQVLEKTGARLVI
GKGGMKEDLLDFFKRFGAAYLAFPGGAALLAAKAIKAVRGVYWLEELGIPEAMWVFEVEKFGPLVVAMDSHGRSLYKEVS
ERAKTKLEELLK
>Mature_492_residues
MLRDEVVNAVVEAIRLAETALPKWVYEKLLDAYERSEGLGKAQLAAILNNIELAVKEKKPMCQDTGLMTFDVIVGDEFPI
KPYEIEELLKEAVRRATREVPIRPNSVDPLSGHNPGDNVGKGVPVTNIKVVPGNTLLIKVRPKGGGSEYPSNLCMIPPSK
GLEGVRECVLKAVFEAGGKPCPPGIVGVAFGGTVEETAKLAKEALYRPEAHEEERVARIEKELLDEVNSLGLGPMGLGGG
PTALAVRADYAYRHPASFPVAVRFNCWAAREAVVEVNADGSWKLVSDNVTPEDLKTPPLEAEDGVEIKLPVEEEEVRKLK
AGDVVYLTGTVVTARDEAHKKIIEEGAPLDLKGLAIYHCGPVVTKEEGRWKVVAAGPTTSARMNALQAQVLEKTGARLVI
GKGGMKEDLLDFFKRFGAAYLAFPGGAALLAAKAIKAVRGVYWLEELGIPEAMWVFEVEKFGPLVVAMDSHGRSLYKEVS
ERAKTKLEELLK

Specific function: Unknown

COG id: COG1951

COG function: function code C; Tartrate dehydratase alpha subunit/Fumarate hydratase class I, N-terminal domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I fumarase family [H]

Homologues:

Organism=Escherichia coli, GI1789443, Length=187, Percent_Identity=42.7807486631016, Blast_Score=168, Evalue=8e-43,
Organism=Escherichia coli, GI1789442, Length=292, Percent_Identity=33.5616438356164, Blast_Score=156, Evalue=3e-39,
Organism=Escherichia coli, GI1787897, Length=468, Percent_Identity=29.9145299145299, Blast_Score=142, Evalue=5e-35,
Organism=Escherichia coli, GI1790564, Length=470, Percent_Identity=28.936170212766, Blast_Score=135, Evalue=8e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004646
- InterPro:   IPR004647
- InterPro:   IPR011167 [H]

Pfam domain/function: PF05681 Fumerase; PF05683 Fumerase_C [H]

EC number: =4.2.1.2 [H]

Molecular weight: Translated: 53356; Mature: 53356

Theoretical pI: Translated: 5.37; Mature: 5.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRDEVVNAVVEAIRLAETALPKWVYEKLLDAYERSEGLGKAQLAAILNNIELAVKEKKP
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHEEEECCCC
MCQDTGLMTFDVIVGDEFPIKPYEIEELLKEAVRRATREVPIRPNSVDPLSGHNPGDNVG
CCHHCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
KGVPVTNIKVVPGNTLLIKVRPKGGGSEYPSNLCMIPPSKGLEGVRECVLKAVFEAGGKP
CCCCCCEEEEECCCEEEEEEECCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHCCCCC
CPPGIVGVAFGGTVEETAKLAKEALYRPEAHEEERVARIEKELLDEVNSLGLGPMGLGGG
CCCCEEEEECCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
PTALAVRADYAYRHPASFPVAVRFNCWAAREAVVEVNADGSWKLVSDNVTPEDLKTPPLE
CCEEEEEECCCCCCCCCCCEEEEEECEECCEEEEEECCCCCEEEEECCCCHHHCCCCCCC
AEDGVEIKLPVEEEEVRKLKAGDVVYLTGTVVTARDEAHKKIIEEGAPLDLKGLAIYHCG
CCCCCEEEECCCHHHHHHHCCCCEEEEEEEEEECCHHHHHHHHHCCCCCCCCCEEEEECC
PVVTKEEGRWKVVAAGPTTSARMNALQAQVLEKTGARLVIGKGGMKEDLLDFFKRFGAAY
CEEECCCCCEEEEEECCCCHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHCCEE
LAFPGGAALLAAKAIKAVRGVYWLEELGIPEAMWVFEVEKFGPLVVAMDSHGRSLYKEVS
EECCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEHHCCCCEEEEECCCCHHHHHHHH
ERAKTKLEELLK
HHHHHHHHHHHC
>Mature Secondary Structure
MLRDEVVNAVVEAIRLAETALPKWVYEKLLDAYERSEGLGKAQLAAILNNIELAVKEKKP
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHEEEECCCC
MCQDTGLMTFDVIVGDEFPIKPYEIEELLKEAVRRATREVPIRPNSVDPLSGHNPGDNVG
CCHHCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
KGVPVTNIKVVPGNTLLIKVRPKGGGSEYPSNLCMIPPSKGLEGVRECVLKAVFEAGGKP
CCCCCCEEEEECCCEEEEEEECCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHCCCCC
CPPGIVGVAFGGTVEETAKLAKEALYRPEAHEEERVARIEKELLDEVNSLGLGPMGLGGG
CCCCEEEEECCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
PTALAVRADYAYRHPASFPVAVRFNCWAAREAVVEVNADGSWKLVSDNVTPEDLKTPPLE
CCEEEEEECCCCCCCCCCCEEEEEECEECCEEEEEECCCCCEEEEECCCCHHHCCCCCCC
AEDGVEIKLPVEEEEVRKLKAGDVVYLTGTVVTARDEAHKKIIEEGAPLDLKGLAIYHCG
CCCCCEEEECCCHHHHHHHCCCCEEEEEEEEEECCHHHHHHHHHCCCCCCCCCEEEEECC
PVVTKEEGRWKVVAAGPTTSARMNALQAQVLEKTGARLVIGKGGMKEDLLDFFKRFGAAY
CEEECCCCCEEEEEECCCCHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHCCEE
LAFPGGAALLAAKAIKAVRGVYWLEELGIPEAMWVFEVEKFGPLVVAMDSHGRSLYKEVS
EECCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEHHCCCCEEEEECCCCHHHHHHHH
ERAKTKLEELLK
HHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8473853 [H]