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The map label for this gene is dphB [H]

Identifier: 156937450

GI number: 156937450

Start: 592533

End: 593255

Strand: Direct

Name: dphB [H]

Synonym: Igni_0657

Alternate gene names: 156937450

Gene position: 592533-593255 (Clockwise)

Preceding gene: 156937449

Following gene: 156937451

Centisome position: 45.67

GC content: 56.98

Gene sequence:

>723_bases
GTGAAGTTCGTGCTGGCCGGGGCCGGCTTGAGGTGCGAACACTTAAACGAAAGGGTCAAGAGGGCCATATTAGAAGCGGA
AGAGGTCTGGGTAGACACCTACACCAGCCCCTTCCCCGGGGGGCTGCTCAACTGCGTAAAGGGGGTAAGGGAGGACGCCT
TAGAGGCCGACAGAAGCGTCTTAGAGGGTCCCTTTAGGTTCTCGAAGAGCGTAACTTTGGTAGTTCCCGGCGACCCCATG
GCCGCGACTGCCCACTCCGCCCTGTTGCTGGAAGCGAGGAGGAGGGGGTACTCCGTTCAGATAATCTCGAACGTCTCGGC
GCTGCAAGCGGCGAGGAGCAAGAGCGGCCTCTCCCAGTACCGCTTCGGCAAGGTAGTCACTTTGATGTATCCTAAGGAGG
GAATCTCCTTCACCGAGTCGGTATATAACGTAATAAAGGATAACGACTCAATGAACTTACATACCATAGTCTTGTTGGAG
ACAGGCTACGGGAGGGACATGACCGTTCCGGAGGCGGCGAGGCTCCTCTGCGAGTACGAAGAGCTCTGCGACCGAAGGGC
GATAGCCATGGCCCGCTTGTGCTGGGACGACGAAACGATAAAGGTAGCCAAGTTAAGGGAACTCGCCGAGTCCGACTTGG
GCGGGCCCCCGCACTTGCTCGTCTTCCCCTCACCCAAGCTCCATCCTATTGAAGAAGAATTTATCGAAGCGGTCCTCAAA
TGA

Upstream 100 bases:

>100_bases
CCATGTTCGTTGCGGCAATGATAGGCGTCGCGGTGGCCGCCGTGGTAGGCGTGGGGGTGGGGCTGTACTTGTGTAAGAGG
TCCTTGAAGGTGGTGAAGGA

Downstream 100 bases:

>100_bases
TACTGACGTGTTCCCCGCACCCCCTTTTTAGCTACACCCCGATACTACGCAGACACCGGGTGAGGGCGTGAGGCTGGTAA
CGGTCAAGATGCCGGAAGCT

Product: diphthine synthase

Products: NA

Alternate protein names: Diphthamide biosynthesis methyltransferase [H]

Number of amino acids: Translated: 240; Mature: 240

Protein sequence:

>240_residues
MKFVLAGAGLRCEHLNERVKRAILEAEEVWVDTYTSPFPGGLLNCVKGVREDALEADRSVLEGPFRFSKSVTLVVPGDPM
AATAHSALLLEARRRGYSVQIISNVSALQAARSKSGLSQYRFGKVVTLMYPKEGISFTESVYNVIKDNDSMNLHTIVLLE
TGYGRDMTVPEAARLLCEYEELCDRRAIAMARLCWDDETIKVAKLRELAESDLGGPPHLLVFPSPKLHPIEEEFIEAVLK

Sequences:

>Translated_240_residues
MKFVLAGAGLRCEHLNERVKRAILEAEEVWVDTYTSPFPGGLLNCVKGVREDALEADRSVLEGPFRFSKSVTLVVPGDPM
AATAHSALLLEARRRGYSVQIISNVSALQAARSKSGLSQYRFGKVVTLMYPKEGISFTESVYNVIKDNDSMNLHTIVLLE
TGYGRDMTVPEAARLLCEYEELCDRRAIAMARLCWDDETIKVAKLRELAESDLGGPPHLLVFPSPKLHPIEEEFIEAVLK
>Mature_240_residues
MKFVLAGAGLRCEHLNERVKRAILEAEEVWVDTYTSPFPGGLLNCVKGVREDALEADRSVLEGPFRFSKSVTLVVPGDPM
AATAHSALLLEARRRGYSVQIISNVSALQAARSKSGLSQYRFGKVVTLMYPKEGISFTESVYNVIKDNDSMNLHTIVLLE
TGYGRDMTVPEAARLLCEYEELCDRRAIAMARLCWDDETIKVAKLRELAESDLGGPPHLLVFPSPKLHPIEEEFIEAVLK

Specific function: Required for the methylation step in diphthamide biosynthesis [H]

COG id: COG1798

COG function: function code J; Diphthamide biosynthesis methyltransferase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the diphthine synthase family [H]

Homologues:

Organism=Homo sapiens, GI117190324, Length=196, Percent_Identity=29.5918367346939, Blast_Score=82, Evalue=3e-16,
Organism=Homo sapiens, GI117190326, Length=197, Percent_Identity=29.4416243654822, Blast_Score=82, Evalue=4e-16,
Organism=Homo sapiens, GI117190262, Length=197, Percent_Identity=29.4416243654822, Blast_Score=82, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17531369, Length=277, Percent_Identity=29.6028880866426, Blast_Score=94, Evalue=6e-20,
Organism=Saccharomyces cerevisiae, GI6323201, Length=184, Percent_Identity=30.4347826086957, Blast_Score=77, Evalue=2e-15,
Organism=Drosophila melanogaster, GI28572120, Length=269, Percent_Identity=28.996282527881, Blast_Score=94, Evalue=6e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR004551 [H]

Pfam domain/function: PF00590 TP_methylase [H]

EC number: =2.1.1.98 [H]

Molecular weight: Translated: 26681; Mature: 26681

Theoretical pI: Translated: 5.72; Mature: 5.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFVLAGAGLRCEHLNERVKRAILEAEEVWVDTYTSPFPGGLLNCVKGVREDALEADRSV
CEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
LEGPFRFSKSVTLVVPGDPMAATAHSALLLEARRRGYSVQIISNVSALQAARSKSGLSQY
HCCCHHCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHCCHHHH
RFGKVVTLMYPKEGISFTESVYNVIKDNDSMNLHTIVLLETGYGRDMTVPEAARLLCEYE
HCCCEEEEEECCCCCHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCHHHHHHHHHHH
ELCDRRAIAMARLCWDDETIKVAKLRELAESDLGGPPHLLVFPSPKLHPIEEEFIEAVLK
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHC
>Mature Secondary Structure
MKFVLAGAGLRCEHLNERVKRAILEAEEVWVDTYTSPFPGGLLNCVKGVREDALEADRSV
CEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
LEGPFRFSKSVTLVVPGDPMAATAHSALLLEARRRGYSVQIISNVSALQAARSKSGLSQY
HCCCHHCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHCCHHHH
RFGKVVTLMYPKEGISFTESVYNVIKDNDSMNLHTIVLLETGYGRDMTVPEAARLLCEYE
HCCCEEEEEECCCCCHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCHHHHHHHHHHH
ELCDRRAIAMARLCWDDETIKVAKLRELAESDLGGPPHLLVFPSPKLHPIEEEFIEAVLK
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11572479 [H]