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The map label for this gene is pqqE [H]

Identifier: 156937423

GI number: 156937423

Start: 566519

End: 567688

Strand: Reverse

Name: pqqE [H]

Synonym: Igni_0630

Alternate gene names: 156937423

Gene position: 567688-566519 (Counterclockwise)

Preceding gene: 156937424

Following gene: 156937422

Centisome position: 43.75

GC content: 52.99

Gene sequence:

>1170_bases
TTGATACCGGTAACTACTATGGTCGTCGGGAGGGGAACGGTCTCTACGAAGATAAAGGGCGAGTACCACCGGAAGAAGCC
CTCCCGGTTCACAGACTACTTCCGCCCCCTCGTGTTCTGGAACATCACTTACCAGTGTAACTTACGTTGCCTTCACTGCT
ACATAAGGGCGAAGGCCCAACAAGACCCCAGCGAGCTGACTACGGAAGAGGCCAAGCGCTTGAGCGAAGAAATGGTTGAA
ATGAGAATACCTTTGGTTATCCTCTCCGGCGGCGAACCCTTGGTTAGGAAGGACTTTTGGGAGATCATCGAACCGATGGC
TAAGGCACAATTTCCGAAACTATCCTTAAGCACCAATGGAACTCTCATAACGAAGGACGTAGCCAGAAGGTTGAAGGATT
ACGGCTTCTCATACGTCGGCATTTCCATAGACTCGATAGTCCCCGCTTACCACGACAAGTTTAGGGGAGTCCCCGGGGCT
TTCAAGGCTACCTTAGAGGGGATAAAGAACAGCGTAGAAGCCGGTCTGGACGTGGGAATAAGGACCACCATAACTCGGTA
CAACGTAAAGGAAGTTCCCGACATAGTCAAGTGGAGCGCCGAGAACGGGATAAAGAGGGTGTCGTTTTACTTACTGGACA
CGATCGGGAGGGGCGAAGAGATAAAGGACTGGCTCCCAACTAAGGAGCAGCTCAAGTGGATGGCTGACGTGGTTATAGAC
CTCGCGCGCGAGTACGCGGACCAGCTTGAAATATTAATAGTTAGGGGGAACTTCATGGGAATTTACATAGCCAGGAAACT
GGCCAAGGACGAGGAGGAGTTCAAGGAGTACCTCAAGATGCTTCAAGCCCAAGGCGACTGCGGCAGGAAGAGCGTCAGCA
TCTATCCCAACGGCGACGTCAAGCCGTGTCAGTTCATAGATTGGGTCACCTTAGGCAACGTCAGGGAGAAGAGCTTGAAG
GAAATACTGAAGAGGGACAACCCCCAACTCGAACCCTTCTTAAACGTAGGGCAGTACCTCAAGGGCAAGTGCGGGCGCTG
TCCTTACAAGGACATCTGCGGCGGCGGCAGCAGAGGTAGGGCTTACGCGATCTTCGGCGACCCGTGGGCCGAGGACCCCT
TGTGCTTCATAGAGCCCGAAGCTCTTGCAAAAGAGTTTTTGGCCGCTTGA

Upstream 100 bases:

>100_bases
CCACCGTTCTTTTGCCGATAATTTGGGCCGGCGTGTTGGCCAAGGTACAAAAGGCGTAGATTTAAACGACAGTGCGCGAG
GCGGACTTGGGTTTTATGAC

Downstream 100 bases:

>100_bases
AGCACGCCGAGAAGGGCAAATCAGATCATGAAGAAGGCTCACAAGCTGACAATAGCGATGGCAACCACGGTCGTAATATA
CTTGCTCTTGCTAGCCTTGA

Product: radical SAM domain-containing protein

Products: NA

Alternate protein names: Pyrroloquinoline quinone biosynthesis protein E [H]

Number of amino acids: Translated: 389; Mature: 389

Protein sequence:

>389_residues
MIPVTTMVVGRGTVSTKIKGEYHRKKPSRFTDYFRPLVFWNITYQCNLRCLHCYIRAKAQQDPSELTTEEAKRLSEEMVE
MRIPLVILSGGEPLVRKDFWEIIEPMAKAQFPKLSLSTNGTLITKDVARRLKDYGFSYVGISIDSIVPAYHDKFRGVPGA
FKATLEGIKNSVEAGLDVGIRTTITRYNVKEVPDIVKWSAENGIKRVSFYLLDTIGRGEEIKDWLPTKEQLKWMADVVID
LAREYADQLEILIVRGNFMGIYIARKLAKDEEEFKEYLKMLQAQGDCGRKSVSIYPNGDVKPCQFIDWVTLGNVREKSLK
EILKRDNPQLEPFLNVGQYLKGKCGRCPYKDICGGGSRGRAYAIFGDPWAEDPLCFIEPEALAKEFLAA

Sequences:

>Translated_389_residues
MIPVTTMVVGRGTVSTKIKGEYHRKKPSRFTDYFRPLVFWNITYQCNLRCLHCYIRAKAQQDPSELTTEEAKRLSEEMVE
MRIPLVILSGGEPLVRKDFWEIIEPMAKAQFPKLSLSTNGTLITKDVARRLKDYGFSYVGISIDSIVPAYHDKFRGVPGA
FKATLEGIKNSVEAGLDVGIRTTITRYNVKEVPDIVKWSAENGIKRVSFYLLDTIGRGEEIKDWLPTKEQLKWMADVVID
LAREYADQLEILIVRGNFMGIYIARKLAKDEEEFKEYLKMLQAQGDCGRKSVSIYPNGDVKPCQFIDWVTLGNVREKSLK
EILKRDNPQLEPFLNVGQYLKGKCGRCPYKDICGGGSRGRAYAIFGDPWAEDPLCFIEPEALAKEFLAA
>Mature_389_residues
MIPVTTMVVGRGTVSTKIKGEYHRKKPSRFTDYFRPLVFWNITYQCNLRCLHCYIRAKAQQDPSELTTEEAKRLSEEMVE
MRIPLVILSGGEPLVRKDFWEIIEPMAKAQFPKLSLSTNGTLITKDVARRLKDYGFSYVGISIDSIVPAYHDKFRGVPGA
FKATLEGIKNSVEAGLDVGIRTTITRYNVKEVPDIVKWSAENGIKRVSFYLLDTIGRGEEIKDWLPTKEQLKWMADVVID
LAREYADQLEILIVRGNFMGIYIARKLAKDEEEFKEYLKMLQAQGDCGRKSVSIYPNGDVKPCQFIDWVTLGNVREKSLK
EILKRDNPQLEPFLNVGQYLKGKCGRCPYKDICGGGSRGRAYAIFGDPWAEDPLCFIEPEALAKEFLAA

Specific function: Together With Moac, Is Involved In The Conversion Of A Guanosine Derivative (Gxp) Into Molybdopterin Precursor Z. [C]

COG id: COG0535

COG function: function code R; Predicted Fe-S oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. PqqE family [H]

Homologues:

Organism=Homo sapiens, GI22027536, Length=140, Percent_Identity=31.4285714285714, Blast_Score=68, Evalue=2e-11,
Organism=Homo sapiens, GI116734668, Length=140, Percent_Identity=31.4285714285714, Blast_Score=68, Evalue=2e-11,
Organism=Escherichia coli, GI1786999, Length=206, Percent_Identity=27.1844660194175, Blast_Score=74, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR000385
- InterPro:   IPR017200
- InterPro:   IPR011843
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 44248; Mature: 44248

Theoretical pI: Translated: 8.57; Mature: 8.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIPVTTMVVGRGTVSTKIKGEYHRKKPSRFTDYFRPLVFWNITYQCNLRCLHCYIRAKAQ
CCCEEEEEEECCCEEEEECCHHHHCCCHHHHHHHHHHEEEEEEEEECCEEEEEEHHHHCC
QDPSELTTEEAKRLSEEMVEMRIPLVILSGGEPLVRKDFWEIIEPMAKAQFPKLSLSTNG
CCHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCHHHHHHHHHHHHHHHCCCCCEEECCCC
TLITKDVARRLKDYGFSYVGISIDSIVPAYHDKFRGVPGAFKATLEGIKNSVEAGLDVGI
EEEEHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCH
RTTITRYNVKEVPDIVKWSAENGIKRVSFYLLDTIGRGEEIKDWLPTKEQLKWMADVVID
HHEEEECCHHHCCHHHHCCCCCCHHHHHHHHHHHCCCCCHHHHHCCCHHHHHHHHHHHHH
LAREYADQLEILIVRGNFMGIYIARKLAKDEEEFKEYLKMLQAQGDCGRKSVSIYPNGDV
HHHHHHCCEEEEEEECCEEEHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCEEEECCCCCC
KPCQFIDWVTLGNVREKSLKEILKRDNPQLEPFLNVGQYLKGKCGRCPYKDICGGGSRGR
CCCCEEEEHHCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCHHHCCCCCCCC
AYAIFGDPWAEDPLCFIEPEALAKEFLAA
EEEEECCCCCCCCEEEECHHHHHHHHHCC
>Mature Secondary Structure
MIPVTTMVVGRGTVSTKIKGEYHRKKPSRFTDYFRPLVFWNITYQCNLRCLHCYIRAKAQ
CCCEEEEEEECCCEEEEECCHHHHCCCHHHHHHHHHHEEEEEEEEECCEEEEEEHHHHCC
QDPSELTTEEAKRLSEEMVEMRIPLVILSGGEPLVRKDFWEIIEPMAKAQFPKLSLSTNG
CCHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCHHHHHHHHHHHHHHHCCCCCEEECCCC
TLITKDVARRLKDYGFSYVGISIDSIVPAYHDKFRGVPGAFKATLEGIKNSVEAGLDVGI
EEEEHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCH
RTTITRYNVKEVPDIVKWSAENGIKRVSFYLLDTIGRGEEIKDWLPTKEQLKWMADVVID
HHEEEECCHHHCCHHHHCCCCCCHHHHHHHHHHHCCCCCHHHHHCCCHHHHHHHHHHHHH
LAREYADQLEILIVRGNFMGIYIARKLAKDEEEFKEYLKMLQAQGDCGRKSVSIYPNGDV
HHHHHHCCEEEEEEECCEEEHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCEEEECCCCCC
KPCQFIDWVTLGNVREKSLKEILKRDNPQLEPFLNVGQYLKGKCGRCPYKDICGGGSRGR
CCCCEEEEHHCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCHHHCCCCCCCC
AYAIFGDPWAEDPLCFIEPEALAKEFLAA
EEEEECCCCCCCCEEEECHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12597275 [H]